Research Topics
| LUIZ EDUARDO BERMUDEZSummaryAffiliation: Oregon State University Country: USA Publications
Research Grants
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Publications
Identification of (+)-erythro-mefloquine as an active enantiomer with greater efficacy than mefloquine against Mycobacterium avium infection in miceLuiz E Bermudez
Kuzell Institute for Arthritis and Infectious Diseases, San Francisco, California, USA
Antimicrob Agents Chemother 56:4202-6. 2012..Thus, (+)-erythro-mefloquine is the active enantiomer of mefloquine against M. avium and perhaps other mycobacteria...- Surface proteome of "Mycobacterium avium subsp. hominissuis" during the early stages of macrophage infectionMichael McNamara
Department of Biomedical Sciences, Department of Chemistry, Oregon State University, Corvallis, Oregon, USA
Infect Immun 80:1868-80. 2012..Additionally, surface-exposed proteins are excellent targets for the host adaptive immune system, and their identification can inform the development of novel treatments, diagnostic tools, and vaccines for mycobacterial disease... - Virulence-related Mycobacterium avium subsp hominissuis MAV_2928 gene is associated with vacuole remodeling in macrophagesSamradhni S Jha
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
BMC Microbiol 10:100. 2010..This mutant, in contrast to the wild-type bacterium, was shown both to have impaired the ability to replicate within macrophages and to have prevented phagosome/lysosome fusion... 
Role of type I cytokines in host defense against Mycobacterium avium infectionLia Danelishvilli
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331 8797, USA
Curr Pharm Des 9:61-5. 2003..avium activity in macrophages. M. avium-specific CD8+ T cells undergo apoptosis early in the infection. Therefore, the immune mechanisms of the host and bacterial strategies for survival are complex and fascinating...- Effects of macrolides and ketolides on mycobacterial infectionsLuiz E Bermudez
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
Curr Pharm Des 10:3221-8. 2004..Telithromycin has a carbamate group linked to an imidazolium and pyridium nucleus at C11-C12. In mice model, both telithromycin and ABT-733 were active in vivo against MAC... - Intracellular phenotype of Mycobacterium avium enters macrophages primarily by a macropinocytosis-like mechanism and survives in a compartment that differs from that with extracellular phenotypeLuiz E Bermudez
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
Cell Biol Int 28:411-9. 2004..These data suggest that uptake of extracellular fluid is part of M. avium IG phenotype uptake by macrophages, and that the IG phenotype inhabits a slightly different vacuole than that of EG... - Thiosemicarbazole (thiacetazone-like) compound with activity against Mycobacterium avium in miceLuiz E Bermudez
Kuzell Institute for Arthritis and Infectious Diseases, California Pacific Medical Center Research Institute, San Francisco, California 94115, USA
Antimicrob Agents Chemother 47:2685-7. 2003..A combination of SRI-286 and moxifloxacin was significantly more active than single drug regimens in liver and spleen... - EDP-420, a bicyclolide (bridged bicyclic macrolide), is active against Mycobacterium aviumLuiz E Bermudez
Department of Biomedical Sciences, College of Veterinary Medicine, 101 Magruder Hall, Oregon State University, Corvallis, OR 97331 4802, USA
Antimicrob Agents Chemother 51:1666-70. 2007..05). Further evaluation of EDP-420 in the treatment of MAC disease is warranted... - Peyer's patch-deficient mice demonstrate that Mycobacterium avium subsp. paratuberculosis translocates across the mucosal barrier via both M cells and enterocytes but has inefficient disseminationLuiz E Bermudez
Kuzell Institute, California Pacific Medical Center Research Institute, San Francisco, California 94115, USA
Infect Immun 78:3570-7. 2010..In summary, M. avium subsp. paratuberculosis interacts with the intestinal mucosa by crossing both Peyer's patches and non-Peyer's patch areas but does not translocate or disseminate efficiently... 
The efficacy of clarithromycin and the bicyclolide EDP-420 against Mycobacterium avium in a mouse model of pulmonary infectionLuiz E Bermudez
Kuzell Institute for Arthritis and Infectious Diseases, California Pacific Medical Center Research Institute, San Francisco, USA
J Infect Dis 197:1506-10. 2008..These results suggest that this animal model can be used to evaluate novel regimens against MAC disease and that compounds with high concentration in the lung might have a significant impact on the outcome of MAC lung disease...- Mefloquine, moxifloxacin, and ethambutol are a triple-drug alternative to macrolide-containing regimens for treatment of Mycobacterium avium diseaseLuiz E Bermudez
Kuzell Institute for Arthritis and Infectious Diseases, California Pacific Medical Center Research Institute, San Francisco, California, USA
J Infect Dis 187:1977-80. 2003..1-log reduction of CLR-R after 4 weeks, and 2.17-log reduction in MAC/mL blood. Treatment of MAC 101 CLR-S strain had comparable results... - CD4+ T cells but Not CD8+ or gammadelta+ lymphocytes are required for host protection against Mycobacterium avium infection and dissemination through the intestinal routeMary Petrofsky
Dept of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
Infect Immun 73:2621-7. 2005..The ingestion of small numbers of M. avium cells induces a protective immune response in the intestines against subsequent infection. However, the bacteria remain viable in intestinal lymph nodes and might disseminate later... - A subinhibitory concentration of clarithromycin inhibits Mycobacterium avium biofilm formationGeorge Carter
Kuzell Institute, California Pacific Medical Center Research Institute, San Francisco, California, USA
Antimicrob Agents Chemother 48:4907-10. 2004..Antibiotics with activity against the bacterium could inhibit biofilm formation. Clarithromycin inhibits biofilm formation but has no activity against established biofilm... - The fadD2 gene is required for efficient Mycobacterium avium invasion of mucosal epithelial cellsTapen Dam
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331 USA
J Infect Dis 193:1135-42. 2006..avium proteins. CONCLUSION: fadD2 is a regulator of M. avium invasion, and its effect is through Cdc42... - A Mycobacterium avium PPE gene is associated with the ability of the bacterium to grow in macrophages and virulence in miceYongjun Li
Kuzell Institute for Arthritis and Infectious Diseases, California Pacific Medical, Center Research Institute San Francisco, CA 94115, USA
Cell Microbiol 7:539-48. 2005..These results revealed a role for this PPE gene in the growth of M. avium in macrophages and in virulence in mice... - In vivo and in vitro growth of Mycobacterium marinum at homoeothermic temperaturesMichael L Kent
Department of Microbiology, Center for Fish Disease Research, Oregon State University, Corvallis, OR 97331 3804, USA
FEMS Microbiol Lett 257:69-75. 2006..Significant differences of growth in vitro and in vivo were observed. All fish strains caused both footpad and deep tissue infections, and two, which grew very poorly or not all at 37 degrees C, proliferated in mammalian macrophages... - Identification of virulence determinants of Mycobacterium avium that impact on the ability to resist host killing mechanismsYong Jun Li
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
J Med Microbiol 59:8-16. 2010..9). Mutants MAV_4292, MAV_0385 and MAV_4264 were susceptible to nitric oxide in vitro. The study of individual mutants can potentially lead to new knowledge about M. avium pathogenic mechanisms... - SRI-286, a thiosemicarbazole, in combination with mefloquine and moxifloxacin for treatment of murine Mycobacterium avium complex diseaseLuiz E Bermudez
Kuzell Institute, California Pacific Medical Center Research Institute, San Francisco, California, USA
Antimicrob Agents Chemother 48:3556-8. 2004..We infected C57 beige mice and treated them with mefloquine, SRI-286, and moxifloxacin. SRI-286 (80 mg/kg) was bactericidal in the liver. Mefloquine plus moxifloxacin or mefloquine plus SRI-286 were better than mefloquine alone... - Identification of Mycobacterium avium genes up-regulated in cultured macrophages and in miceLia Danelishvili
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
FEMS Microbiol Lett 239:41-9. 2004..Evaluation of most expressed genes in M. smegmatis confirmed their up-regulation in M. avium after 24 h infection of macrophages in vitro and mice... - Mycobacterium tuberculosis uptake by recipient host macrophages is influenced by environmental conditions in the granuloma of the infectious individual and is associated with impaired production of interleukin-12 and tumor necrosis factor alphaYong-jun Li
Kuzell Institute for Arthritis and Infectious Diseases, California Pacific Medical Center Research Institute, San Francisco, California 94115, USA
Infect Immun 70:6223-30. 2002..The results suggest that M. tuberculosis in vivo may be adapted to gain access to the intracellular environment in a very efficient fashion and may do so by using different receptors from the complement and mannose receptors... - Characterization of biofilm formation by clinical isolates of Mycobacterium aviumGeorge Carter
Laboratory of Bacterial Pathogenesis, Kuzell Institute and Liposome Research Laboratory, California Pacific Medical Center Research Institute, San Francisco, CA, USA
J Med Microbiol 52:747-52. 2003..Identification of M. avium genes involved in biofilm formation and further studies of the effect of antimicrobials on the establishment of biofilm may identify approaches for inhibiting M. avium biofilm formation and colonization... - Elemental analysis of Mycobacterium avium-, Mycobacterium tuberculosis-, and Mycobacterium smegmatis-containing phagosomes indicates pathogen-induced microenvironments within the host cell's endosomal systemDirk Wagner
Kuzell Institute for Arthritis and Infectious Diseases, San Francisco, CA 94115, USA
J Immunol 174:1491-500. 2005..tuberculosis vacuoles exhibited retarded acquisition of iron compared with phagosomes with wild-type M. tuberculosis. This is a unique approach to define the environmental conditions within the pathogen-containing compartment... - The ability to form biofilm influences Mycobacterium avium invasion and translocation of bronchial epithelial cellsYoshitaka Yamazaki
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
Cell Microbiol 8:806-14. 2006..Mutants had impaired ability to cause infection in mice, as well. The ability to form biofilm appeared to be associated with the invasiveness of MAC A5... - Genomic approach to identifying the putative target of and mechanisms of resistance to mefloquine in mycobacteriaLia Danelishvili
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, Corvallis, OR 97331-8797, USA
Antimicrob Agents Chemother 49:3707-14. 2005..avium genes associated with M. smegmatis resistant to mefloquine were sequenced. Two groups of genes were identified: one affecting membrane transport and one gene that apparently is involved in regulation of cellular replication... - Mycobacterium avium infection of macrophages results in progressive suppression of interleukin-12 production in vitro and in vivoDirk Wagner
Laboratory of Bacterial Pathogenesis, Kuzell Institute for Arthritis and Infectious Diseases, California Pacific Medical Center Research Institute, San Francisco, California 94115, USA
J Leukoc Biol 71:80-8. 2002..Our data indicate that M. avium infection of macrophages suppresses IL-12 production by infected cells and that the suppression was not a result of the presence of IL-10 and TGF beta 1 in the culture supernatant... - A Mycobacterium avium subsp. paratuberculosis LuxR regulates cell envelope and virulenceMarta Alonso-Hearn
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon 97331, USA
Innate Immun 16:235-47. 2010..Here we show that LuxR regulates virulence determinants and is involved in mycobacteria adaptation to the host... - Mycobacterium avium genes MAV_5138 and MAV_3679 are transcriptional regulators that play a role in invasion of epithelial cells, in part by their regulation of CipA, a putative surface protein interacting with host cell signaling pathwaysMelanie J Harriff
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon 97331, USA
J Bacteriol 191:1132-42. 2009..avium complex have a novel mechanism for activating cytoskeletal rearrangement, prompting uptake by host epithelial cells, and that this mechanism is regulated in part by fadD2, MAV_5138, and MAV_3679... - Pathogenesis of nontuberculous mycobacteria infectionsJeffery McGarvey
Kuzell Institute, California Pacific Medical Center Research Institute, 2200 Webster Street, Suite 305, San Francisco, CA 94115, USA
Clin Chest Med 23:569-83. 2002..It turns out that M avium is a fascinating pathogen after all. The increasing knowledge about M avium pathogenesis may one day provide means for a more effective prophylaxis as well as for treatment of the infection... - Species of environmental mycobacteria differ in their abilities to grow in human, mouse, and carp macrophages and with regard to the presence of mycobacterial virulence genes, as observed by DNA microarray hybridizationMelanie J Harriff
Department of Biomedical Sciences, College of Veterinary Medicine, 105 Magruder, Oregon State University, Corvallis, OR 97331, USA
Appl Environ Microbiol 74:275-85. 2008..Further understanding of the role these genes play in host specificity and pathogenicity will be important to gain insight into the zoonotic potential of certain EM as well as their mechanisms of virulence... - Identification of Mycobacterium avium pathogenicity island important for macrophage and amoeba infectionLia Danelishvili
Department of Biomedical Sciences, College of Veterinary Medicine, and Microbiology, Oregon State University, Corvallis, OR 97331, USA
Proc Natl Acad Sci U S A 104:11038-43. 2007..The defect in the ability of the mutants to invade both cells was highly similar, suggesting that M. avium might have evolved mechanisms that are used to enter amoebas and human macrophages... - The ability of Mycobacterium avium subsp. paratuberculosis to enter bovine epithelial cells is influenced by preexposure to a hyperosmolar environment and intracellular passage in bovine mammary epithelial cellsDilip Patel
Department of Food Science, Oregon State University, Corvallis, OR 97331, USA
Infect Immun 74:2849-55. 2006..001). Microarray analysis of intracellular M. avium subsp. paratuberculosis RNA indicates the increased transcription of genes which might be associated with an invasive phenotype... - Secreted Mycobacterium tuberculosis Rv3654c and Rv3655c proteins participate in the suppression of macrophage apoptosisLia Danelishvili
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, United States of America
PLoS ONE 5:e10474. 2010..The mechanisms by which M. tuberculosis H37Rv strain suppress apoptosis and escapes human macrophage killing was investigated... - Identification of Mycobacterium avium genes that affect invasion of the intestinal epitheliumElizabeth Miltner
Kuzell Institute for Arthritis and Infectious Diseases, San Francisco, California, USA
Infect Immun 73:4214-21. 2005.... - In vivo efficacy of phage therapy for Mycobacterium avium infection as delivered by a nonvirulent mycobacteriumLia Danelishvili
Department of Biomedical Sciences, College of Veterinary Medicine, Department of Microbiology, College of Science, Oregon State University, 105 Magruder Hall, Corvallis, OR 97331-8797, USA
Microb Drug Resist 12:1-6. 2006..These in vivo studies confirmed the in vitro findings that an avirulent mycobacterium can be used as a carrier to deliver antimycobacterial phage intracellularly... - Mycobacterium tuberculosis infection causes different levels of apoptosis and necrosis in human macrophages and alveolar epithelial cellsLia Danelishvili
Kuzell Institute for Arthritis and Infectious Diseases, California Pacific Medical Center Research Institute, San Francisco, CA 94115, USA
Cell Microbiol 5:649-60. 2003..Our results showed that the differential induction of apoptosis between macrophages and alveolar epithelial cells represents specific strategies of M. tuberculosis for survival in the host... - The efficiency of the translocation of Mycobacterium tuberculosis across a bilayer of epithelial and endothelial cells as a model of the alveolar wall is a consequence of transport within mononuclear phagocytes and invasion of alveolar epithelial cellsLuiz E Bermudez
Kuzell Institute for Arthritis and Infectious Diseases, California Pacific Medical Center Research Institute, University of California, San Francisco, California, USA
Infect Immun 70:140-6. 2002..We identified two potential mechanisms by which M. tuberculosis gains access to deeper tissues, by translocating across epithelial cells and by traveling into the blood vessels within monocytes... - Mimicry of the pathogenic mycobacterium vacuole in vitro elicits the bacterial intracellular phenotype, including early-onset macrophage deathJulie Early
Molecular and Cellular Biology Program, Oregon State University, Corvallis, Oregon 97331, USA
Infect Immun 79:2412-22. 2011.... - Mycobacterium avium genes associated with the ability to form a biofilmYoshitaka Yamazaki
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, Corvallis, OR 97331, USA
Appl Environ Microbiol 72:819-25. 2006..In conclusion, it appears that GPL biosynthesis, including the GDP-mannose biosynthesis pathway, is the most important pathway involved in the production of M. avium biofilm... - The Mycobacterium avium subsp. paratuberculosis MAP3464 gene encodes an oxidoreductase involved in invasion of bovine epithelial cells through the activation of host cell Cdc42Marta Alonso-Hearn
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331 4802, USA
Infect Immun 76:170-8. 2008..These findings reveal a novel signaling pathway activated during M. avium subsp. paratuberculosis entry that links the product of MAP3464 gene to activation of Cdc42 in the host cell... - Mycobacterium avium genes upregulated upon infection of Acanthamoeba castellanii demonstrate a common response to the intracellular environmentRachel Tenant
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA
Curr Microbiol 52:128-33. 2006..These results showed that the GFP promoter library in M. smegmatis is a valid system for studying gene upregulation in M. avium systems, and that many M. avium genes are commonly upregulated following macrophage and amoeba infection... - Genetic and phenotypic differences between Legionella pneumophila strainsMustapha M Samrakandi
Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Lincoln, Nebraska 68583, USA
J Clin Microbiol 40:1352-62. 2002..These studies emphasize the importance of further investigation into the genetic makeup of these strains, which is likely to lead to the identification of additional factors involved in Legionella pathogenesis... - A Mycobacterium marinum mel2 mutant is defective for growth in macrophages that produce reactive oxygen and reactive nitrogen speciesSelvakumar Subbian
Department of Microbial and Molecular Pathogenesis, Texas A and M University Health Sciences Center, 471 Reynolds Medical Building, College Station, TX 77843, USA
Infect Immun 75:127-34. 2007..These observations demonstrate that the M. marinum mel2 locus plays a role in resistance to ROS and RNS produced by activated macrophages... - Clinical, microbiological, and immunological characteristics in HIV-infected subjects at risk for disseminated Mycobacterium avium complex disease: an AACTG studyRob Roy MacGregor
Infectious Diseases Division, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
AIDS Res Hum Retroviruses 21:689-95. 2005..MAC-specific T cell responses apparently develop and persist during DMAC, but are dysfunctional or too infrequent to prevent persistence... - Killing of Mycobacterium avium and Mycobacterium tuberculosis by a mycobacteriophage delivered by a nonvirulent mycobacterium: a model for phage therapy of intracellular bacterial pathogensLawrence Broxmeyer
Med-America Research, Whitestone, New York, USA
J Infect Dis 186:1155-60. 2002..In addition, the M. smegmatis vacuole harboring TM4 fuses with the M. avium vacuole in macrophages. These results suggest a potentially novel concept to kill intracellular pathogenic bacteria and warrant future development... - Elemental analysis of the Mycobacterium avium phagosome in Balb/c mouse macrophagesDirk Wagner
Division of Infectious Diseases, Department of Internal Medicine, University of Freiburg, Freiburg, Germany
Biochem Biophys Res Commun 344:1346-51. 2006..We hypothesize that a potassium channel is abundant in the phagosome in macrophages that may be related to microbiocidal killing, similar to the requirement of potassium channels for microbiocidal function in neutrophils... - fecB, a gene potentially involved in iron transport in Mycobacterium avium, is not induced within macrophagesDirk Wagner
Division of Infectious Diseases, Department of Internal Medicine II, University of Freiburg, Hugstetter Strasse 55, D 79106 Freiburg, Germany
FEMS Microbiol Lett 247:185-91. 2005..In macrophages, M. avium fec B was not induced during the early phase of infection, suggesting that the iron concentration in the mycobacterial phagosome is not sufficiently low to stimulate the expression of fecB in M. avium... - Differential virulence of Mycobacterium avium strains isolated from HIV-infected patients with disseminated M. avium complex diseaseKiyofumi Ohkusu
Department of Pathology and Laboratory Medicine, Childrens Hospital Los Angeles and University of Southern California, Los Angeles, California 90027, USA
J Infect Dis 190:1347-54. 2004..There were significant differences in the virulence properties, and these differences may provide insight into the interplay between microbial pathogenesis and host defense... - Virulent mycobacteria and the many aspects of macrophage uptakeLia Danelishvili
Future Microbiol 2:461-4. 2007
Research Grants
- Genes Associated with Mycobacterium avium PathogenesisLuiz Bermudez; Fiscal Year: 2007..An initial screen of 3000 mutants resulted in the identification of a number of virulent determinants, such as PPE genes, polyketide synthases, MmpL proteins, ABC transporters, and several genes of unknown function. ..
- Post Translation Regulation in MycobacteriaLuiz Bermudez; Fiscal Year: 2007..This work has the potential to unveil mechanisms used by mycobacteria to regulate virulence genes in the environment of the host. In case we confirm our hypothesis, future work will address the role of Ion in M. tuberculosis ..
- MYCOBACTERIAL ENVIRONMENT WITHIN MACROPHAGESLuiz Bermudez; Fiscal Year: 2003..We believe that the proposed studies will generate important information about the phagosome environment and the mycobacterial response to it. ..
- GENES ASSOCIATED WITH M AVIUM PATHOGENESISLuiz Bermudez; Fiscal Year: 2002..The applicants believe that the studies outlined in this proposal will provide excellent tools for the identification of novel genes associated with mechanisms of pathogenesis of M. avium.m. ..
- Target for Mefloquine in MycobacteriaLuiz Bermudez; Fiscal Year: 2002..avium is exposed to mefloquine. This work, focused on an active anti-mycobacterial compound, has the potential to unveil new target(s) in both M. avium and M. tuberculosis. ..
- Genes Associated with Mycobacterium avium PathogenesisLUIZ EDUARDO BERMUDEZ; Fiscal Year: 2010..M. avium forms biofilm and lives within macrophages. Only a few antimicrobials have activity against it. The knowledge about pathogenic mechanisms of infection is needed to improve effective therapy. ..