R P Joshi

Summary

Affiliation: Old Dominion University
Country: USA

Publications

  1. ncbi Nanosecond pulsed electric fields modulate cell function through intracellular signal transduction mechanisms
    Stephen J Beebe
    Center for Pediatric Research, Eastern Virginia Medical School, Children s Hospital for The King s Daughters, Norfolk, VA, USA
    Physiol Meas 25:1077-93. 2004
  2. doi Simulation studies of ultrashort, high-intensity electric pulse induced action potential block in whole-animal nerves
    Ravindra P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 0246, USA
    IEEE Trans Biomed Eng 55:1391-8. 2008
  3. doi Case for applying subnanosecond high-intensity, electrical pulses to biological cells
    Ravindra P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 0246, USA
    IEEE Trans Biomed Eng 58:2860-6. 2011
  4. ncbi Bioelectric effects of intense ultrashort pulses
    R P Joshi
    Department of Electrical and Computer Engineering, and Frank Reidy Research Center for Bioelectrics, Old Domonion University, Norfolk, VA 23529 0246, USA
    Crit Rev Biomed Eng 38:255-304. 2010
  5. doi Analysis of cell membrane permeabilization mechanics and pore shape due to ultrashort electrical pulsing
    Ravindra P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA
    Med Biol Eng Comput 48:837-44. 2010
  6. doi Model study of time-dependent muscle response to pulsed electrical stimulation
    Ravindra P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia, USA
    Bioelectromagnetics 31:361-70. 2010
  7. ncbi Microscopic calculations of local lipid membrane permittivities and diffusion coefficients for application to electroporation analyses
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 0246, USA
    Biochem Biophys Res Commun 348:643-8. 2006
  8. ncbi Self-consistent simulations of electroporation dynamics in biological cells subjected to ultrashort electrical pulses
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia 23529-0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 64:011913. 2001
  9. ncbi Simulations of nanopore formation and phosphatidylserine externalization in lipid membranes subjected to a high-intensity, ultrashort electric pulse
    Q Hu
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia 23529 0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 72:031902. 2005
  10. ncbi Simulations of intracellular calcium release dynamics in response to a high-intensity, ultrashort electric pulse
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 75:041920. 2007

Collaborators

Detail Information

Publications23

  1. ncbi Nanosecond pulsed electric fields modulate cell function through intracellular signal transduction mechanisms
    Stephen J Beebe
    Center for Pediatric Research, Eastern Virginia Medical School, Children s Hospital for The King s Daughters, Norfolk, VA, USA
    Physiol Meas 25:1077-93. 2004
    ....
  2. doi Simulation studies of ultrashort, high-intensity electric pulse induced action potential block in whole-animal nerves
    Ravindra P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 0246, USA
    IEEE Trans Biomed Eng 55:1391-8. 2008
    ..The predictions are compared to actual 600-ns experimental reports on rats and shown to be in very good agreement. Effective strength-duration plots for neuromuscular incapacitation are also generated...
  3. doi Case for applying subnanosecond high-intensity, electrical pulses to biological cells
    Ravindra P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 0246, USA
    IEEE Trans Biomed Eng 58:2860-6. 2011
    ..This temporal regime would be used, for example, in the electrotherapy of mixed cell ensembles having different dielectric response properties...
  4. ncbi Bioelectric effects of intense ultrashort pulses
    R P Joshi
    Department of Electrical and Computer Engineering, and Frank Reidy Research Center for Bioelectrics, Old Domonion University, Norfolk, VA 23529 0246, USA
    Crit Rev Biomed Eng 38:255-304. 2010
    ..Relevant experimental data are also presented, and some of the many potential bioengineering applications discussed...
  5. doi Analysis of cell membrane permeabilization mechanics and pore shape due to ultrashort electrical pulsing
    Ravindra P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA
    Med Biol Eng Comput 48:837-44. 2010
    ..Furthermore, we show that the pore shape and membrane conduction would be dynamic, and evolve toward a symmetric characteristic over time. This duration has been shown to be in the micro-second range...
  6. doi Model study of time-dependent muscle response to pulsed electrical stimulation
    Ravindra P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia, USA
    Bioelectromagnetics 31:361-70. 2010
    ..Other possibilities such as calcium release at nerve end endings also exist. It is also proposed that multipulsing strategies and changing the electric field direction by using multielectrode systems would be useful...
  7. ncbi Microscopic calculations of local lipid membrane permittivities and diffusion coefficients for application to electroporation analyses
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 0246, USA
    Biochem Biophys Res Commun 348:643-8. 2006
    ..Permittivities are also calculated and shown to be dramatically non-uniform in the vicinity of membranes under high external fields. A positive feedback mechanism leading to enhanced membrane fields is discussed...
  8. ncbi Self-consistent simulations of electroporation dynamics in biological cells subjected to ultrashort electrical pulses
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia 23529-0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 64:011913. 2001
    ..coli cells. The time constant for the longer process also matches experiments. The study suggests that controlled manipulation of the pore "open times" can be achieved through multiple, ultrashort pulses...
  9. ncbi Simulations of nanopore formation and phosphatidylserine externalization in lipid membranes subjected to a high-intensity, ultrashort electric pulse
    Q Hu
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia 23529 0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 72:031902. 2005
    ..Finally, the simulations confirm that PS externalization is a nanopore facilitated event, rather than the result of molecular translocation across the trans-membrane energy barrier...
  10. ncbi Simulations of intracellular calcium release dynamics in response to a high-intensity, ultrashort electric pulse
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 75:041920. 2007
    ..A quantitative explanation for the lack of such oscillatory behavior, based on the density dependent calcium fluxes, is also provided...
  11. ncbi Simulations of transient membrane behavior in cells subjected to a high-intensity ultrashort electric pulse
    Q Hu
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia 23529 0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 71:031914. 2005
    ..This could be of practical importance and have direct relevance to the recent observations of calcium release from the endoplasmic reticulum in cells subjected to such ultrashort, high-intensity pulses...
  12. ncbi Self-consistent analyses for potential conduction block in nerves by an ultrashort high-intensity electric pulse
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia 23529 0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 75:061906. 2007
    ..This is self-consistently coupled with a distributed circuit representation of the nerve dynamics. Our results indicate that poration at a single neural segment would be sufficient to produce an observable, yet reversible, effect...
  13. ncbi Improved energy model for membrane electroporation in biological cells subjected to electrical pulses
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia 23529 0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 65:041920. 2002
    ..This theory can be augmented to include pore-pore interactions to move beyond the independent pore picture...
  14. ncbi Energy-landscape-model analysis for irreversibility and its pulse-width dependence in cells subjected to a high-intensity ultrashort electric pulse
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia 23529 0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 69:051901. 2004
    ..The model captures the essence of the experimentally observed pulse-width dependence, and provides a possible physical picture that depends only on the electrical trigger. A number of interesting features are predicted...
  15. ncbi Mechanism for membrane electroporation irreversibility under high-intensity, ultrashort electrical pulse conditions
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia 23529 0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 66:052901. 2002
    ..g., mitochondria), without irreversible shock at the outer membranes, by a nanosecond, high-intensity electric pulse. This mechanism also explains the greater damage from multiple electric shocks...
  16. doi Synergistic effects of local temperature enhancements on cellular responses in the context of high-intensity, ultrashort electric pulses
    J Song
    Department of Electrical and Computer Engineering and Frank Reidy Center for Bio Electrics, Old Dominion University, Norfolk, VA 23529 0246, USA
    Med Biol Eng Comput 49:713-8. 2011
    ..Our study thus suggests that the use of repetitive high-intensity voltage pulses could open up possibilities for a host of synergistic bio-responses involving both thermal and electrically driven phenomena...
  17. ncbi Theoretical predictions of electromechanical deformation of cells subjected to high voltages for membrane electroporation
    R P Joshi
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, Virginia 23529 0246, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 65:021913. 2002
    ..Finally, it is shown that the bending moments are generally quite small and can be neglected for a simpler analysis...
  18. doi Cellular apoptosis by nanosecond, high-intensity electric pulses: model evaluation of the pulsing threshold and extrinsic pathway
    Jiahui Song
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 0246, USA
    Bioelectrochemistry 79:179-86. 2010
    ..Finally, our results suggest that multi-needle electrode systems with adjustable field orientations would likely enhance apoptosis in the context of pulsed voltage-induced inactivation of tumor cells...
  19. ncbi Leukemic cell intracellular responses to nanosecond electric fields
    Nianyong Chen
    Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA
    Biochem Biophys Res Commun 317:421-7. 2004
    ..The results suggest application of this ultrashort pulse technology to modulate nuclear structure and function for potential therapeutic benefit...
  20. ncbi Nanosecond electric pulses penetrate the nucleus and enhance speckle formation
    Nianyong Chen
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA
    Biochem Biophys Res Commun 364:220-5. 2007
    ..The resulting nuclear speckle changes were also cell cycle dependent. These findings suggest that 10ns pulses directly influenced nuclear processes, such as the changes in the nuclear RNA-protein complexes...
  21. ncbi Long-lasting plasma membrane permeabilization in mammalian cells by nanosecond pulsed electric field (nsPEF)
    Andrei G Pakhomov
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, USA
    Bioelectromagnetics 28:655-63. 2007
    ..The phenomenon of long-lived membrane permeabilization provides new insights on the nature of nsPEF-opened conductance pores and on molecular mechanisms that underlie nsPEF bioeffects...
  22. ncbi Membrane permeabilization and cell damage by ultrashort electric field shocks
    Andrei G Pakhomov
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA
    Arch Biochem Biophys 465:109-18. 2007
    ..We conclude that plasma membrane permeabilization is the principal cause of necrotic transformation in nsPEF-exposed cells and probably contributes to other known nsPEF bioeffects...
  23. ncbi Ultrashort electric pulse induced changes in cellular dielectric properties
    Allen L Garner
    Bioelectromagnetism Laboratory, Department of Nuclear Engineering and Radiological Sciences, University of Michigan, Ann Arbor, MI 48109, USA
    Biochem Biophys Res Commun 362:139-44. 2007
    ..This data could be used in models describing the phenomena at work...