Richard Heller

Summary

Affiliation: Old Dominion University
Country: USA

Publications

  1. pmc Electrically mediated delivery of plasmid DNA to the skin, using a multielectrode array
    Richard Heller
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
    Hum Gene Ther 21:357-62. 2010
  2. pmc Topical gene electrotransfer to the epidermis of hairless guinea pig by non-invasive multielectrode array
    Siqi Guo
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, United States of America
    PLoS ONE 8:e73423. 2013
  3. ncbi request reprint Delivery of Interleukin-15 to B16 Melanoma by Electroporation Leads to Tumor Regression and Long-term Survival
    B Marrero
    Department of Molecular Medicine, University of South Florida, Tampa, Florida 33613, USA
    Technol Cancer Res Treat 13:551-60. 2014
  4. pmc Electroporation-mediated gene transfer directly to the swine heart
    B Hargrave
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
    Gene Ther 20:151-7. 2013
  5. pmc Evaluation of delivery conditions for cutaneous plasmid electrotransfer using a multielectrode array
    B Ferraro
    Department of Molecular Medicine, University of South Florida, Tampa, FL, USA
    Gene Ther 18:496-500. 2011
  6. pmc Evaluation of a novel non-penetrating electrode for use in DNA vaccination
    Amy Donate
    College of Medicine, University of South Florida, Tampa, Florida, United States of America
    PLoS ONE 6:e19181. 2011
  7. doi request reprint Electroporation based gene therapy--from the bench to the bedside
    Richard Heller
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
    Conf Proc IEEE Eng Med Biol Soc 2011:736-8. 2011
  8. pmc Electro-gene transfer to skin using a noninvasive multielectrode array
    Siqi Guo
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA
    J Control Release 151:256-62. 2011
  9. ncbi request reprint Evaluation of toxicity following electrically mediated interleukin-12 gene delivery in a B16 mouse melanoma model
    Loree Heller
    Department of Medical Microbiology and Immunology, University of South Florida, Tampa 33612, USA
    Clin Cancer Res 12:3177-83. 2006
  10. ncbi request reprint Electrochemotherapy for the treatment of human sarcoma in athymic rats
    Mark J Jaroszeski
    Department of Chemical Engineering, College of Engineering, University of South Florida, Tampa, FL 33612, USA
    Technol Cancer Res Treat 1:393-9. 2002

Collaborators

Detail Information

Publications34

  1. pmc Electrically mediated delivery of plasmid DNA to the skin, using a multielectrode array
    Richard Heller
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
    Hum Gene Ther 21:357-62. 2010
    ..This is important, as it will facilitate the translation of electroporation-mediated gene delivery to skin for clinical use with DNA vaccines or for therapies for cancer or protein deficiencies...
  2. pmc Topical gene electrotransfer to the epidermis of hairless guinea pig by non-invasive multielectrode array
    Siqi Guo
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, United States of America
    PLoS ONE 8:e73423. 2013
    ..These results suggest gene electrotransfer with the MEA can be a safe, efficient, non-invasive skin delivery method for skin disorders, vaccinations and potential systemic diseases where low levels of gene products are sufficient. ..
  3. ncbi request reprint Delivery of Interleukin-15 to B16 Melanoma by Electroporation Leads to Tumor Regression and Long-term Survival
    B Marrero
    Department of Molecular Medicine, University of South Florida, Tampa, Florida 33613, USA
    Technol Cancer Res Treat 13:551-60. 2014
    ....
  4. pmc Electroporation-mediated gene transfer directly to the swine heart
    B Hargrave
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
    Gene Ther 20:151-7. 2013
    ..Our results provide evidence that in vivo electroporation can be a safe and effective non-viral method for delivering genes to the heart, in vivo...
  5. pmc Evaluation of delivery conditions for cutaneous plasmid electrotransfer using a multielectrode array
    B Ferraro
    Department of Molecular Medicine, University of South Florida, Tampa, FL, USA
    Gene Ther 18:496-500. 2011
    ..Our results indicate that the design of the MEA is a viable option for cutaneous plasmid DNA delivery by in vivo EP to a large surface area...
  6. pmc Evaluation of a novel non-penetrating electrode for use in DNA vaccination
    Amy Donate
    College of Medicine, University of South Florida, Tampa, Florida, United States of America
    PLoS ONE 6:e19181. 2011
    ..While this electrode requires further study, our results suggest that the MEA has potential for use in electrically mediated intradermal DNA vaccination...
  7. doi request reprint Electroporation based gene therapy--from the bench to the bedside
    Richard Heller
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
    Conf Proc IEEE Eng Med Biol Soc 2011:736-8. 2011
    ..It is clear that this delivery approach has tremendous potential to facilitate the translation of gene transfer protocols from the bench to the bedside...
  8. pmc Electro-gene transfer to skin using a noninvasive multielectrode array
    Siqi Guo
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA
    J Control Release 151:256-62. 2011
    ..These results suggest that EP with MEA can be an efficient and non-invasive skin delivery method with less adverse side effects than other EP delivery systems and promising clinical applications...
  9. ncbi request reprint Evaluation of toxicity following electrically mediated interleukin-12 gene delivery in a B16 mouse melanoma model
    Loree Heller
    Department of Medical Microbiology and Immunology, University of South Florida, Tampa 33612, USA
    Clin Cancer Res 12:3177-83. 2006
    ..To translate this observation to the clinic, an evaluation of toxicity was done in the mouse model...
  10. ncbi request reprint Electrochemotherapy for the treatment of human sarcoma in athymic rats
    Mark J Jaroszeski
    Department of Chemical Engineering, College of Engineering, University of South Florida, Tampa, FL 33612, USA
    Technol Cancer Res Treat 1:393-9. 2002
    ..9% durable complete responses; ECT that employed bleomycin resulted in the highest antitumor effects. This indicates the feasibility of electrochemotherapy as a modality for limb preserving treatments for sarcoma of the extremities...
  11. ncbi request reprint IL-12 gene therapy using an electrically mediated nonviral approach reduces metastatic growth of melanoma
    M Lee Lucas
    Department of Medical Microbiology and Immunology, University of South Florida, Tampa, Florida 33612, USA
    DNA Cell Biol 22:755-63. 2003
    ..5% of mice in the no-treatment group. The results show that administration of a plasmid encoding IL-12 with electroporation has a therapeutic effect on primary tumors as well as distant tumors and metastases...
  12. ncbi request reprint Effect of electrically mediated intratumor and intramuscular delivery of a plasmid encoding IFN alpha on visible B16 mouse melanomas
    Loree C Heller
    University of South Florida, Center for Molecular Delivery, Department of Surgery MDC16, 12901 Bruce B Downs Blvd, Tampa, FL 33612, USA
    Technol Cancer Res Treat 1:205-9. 2002
    ..Intratumoral delivery of interferon alpha plasmid not only slows melanoma growth, but induces complete, long term, regression. This effect was not observed after intramuscular delivery...
  13. ncbi request reprint Complete regression of established subcutaneous B16 murine melanoma tumors after delivery of an HIV-1 Vpr-expressing plasmid by in vivo electroporation
    Andrea N McCray
    Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa, FL 33612, USA
    Mol Ther 14:647-55. 2006
    ....
  14. ncbi request reprint Electrically mediated plasmid DNA delivery to solid tumors in vivo
    Mark J Jaroszeski
    Department of Chemical Engineering, College of Engineering, University of South Florida, Tampa, FL, USA
    Methods Mol Biol 245:237-44. 2004
  15. ncbi request reprint Enhancement of anti-melanoma activity of a plasmid expressing HIV-1 Vpr delivered through in vivo electroporation
    Andrea N McCray
    Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, Florida, USA
    Cancer Biol Ther 6:1269-75. 2007
    ..In addition, it appeared that this enhanced anti-tumor effect was correlated with prolonged Vpr expression and the associated induction of intratumoral apoptosis...
  16. ncbi request reprint Electroporation gene therapy preclinical and clinical trials for melanoma
    Loree C Heller
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, 4211 Monarch Way, Suite 300, Norfolk, VA 23508, USA
    Curr Gene Ther 10:312-7. 2010
    ..This review discusses preclinically tested electroporation gene therapies for melanoma with clinical potential and the conversion of these therapies to clinical trials...
  17. doi request reprint Transfection by electroporation
    Huntington Potter
    Byrd Alzheimer s Institute, University of South Florida College of Medicine, Tampa, Florida, USA
    Curr Protoc Cell Biol . 2011
    ..Protocols are described for the use of electroporation in vivo to perform gene therapy for cancer therapy and DNA vaccination. Also described are modifications for preparation and transfection of plant protoplasts...
  18. pmc The use of an in vitro 3D melanoma model to predict in vivo plasmid transfection using electroporation
    BERNADETTE MARRERO
    Department of Molecular Medicine, University of South Florida, Tampa, FL 33612, USA
    Biomaterials 33:3036-46. 2012
    ....
  19. pmc Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma
    Adil I Daud
    Cutaneous Oncology and Experimental Therapeutics Programs, H Lee Moffitt Cancer Center, University of South Florida, Tampa, FL, USA
    J Clin Oncol 26:5896-903. 2008
    ..In vivo electroporation, in preclinical models, significantly enhances gene transfer efficiency while retaining the safety advantages of plasmid DNA...
  20. ncbi request reprint Electroporation for targeted gene transfer
    Loree C Heller
    University of South Florida, Center of Molecular Delivery, Department of Medical Microbiology and Immunology, Center for Molecular Delivery, College of Medicine, Tampa, 33612 4799, USA
    Expert Opin Drug Deliv 2:255-68. 2005
    ..Although clinical trials using gene transfer with in vivo electroporation have not as yet been realised, the tremendous growth of this technology suggests that the first trials will soon be initiated...
  21. ncbi request reprint Effect of electrochemotherapy on muscle and skin
    Richard Heller
    Center for Molecular Delivery, University of South Florida, Tampa, FL 33612, USA
    Technol Cancer Res Treat 1:385-92. 2002
    ..The treated sites were examined histologically 3, 14 and 56 days later. Limb function was not affected by the treatment and skin and muscle necrosis was only seen at the higher doses...
  22. ncbi request reprint IL-12 plasmid delivery by in vivo electroporation for the successful treatment of established subcutaneous B16.F10 melanoma
    M Lee Lucas
    Department of Medical Microbiology and Immunology Institute for Biomolecular Science, University of South Florida, Tampa, Florida 33612, USA
    Mol Ther 5:668-75. 2002
    ..Neither i.m. nor i.t. treatment was successful against B16.F10 tumors in a nude mouse model, supporting a role for T cells in regression of this tumor model...
  23. ncbi request reprint Electric field enhanced plasmid delivery to liver hepatocellular carcinomas
    Richard Gilbert
    Center for Molecular Delivery, University of South Florida, USA
    Technol Cancer Res Treat 1:355-64. 2002
    ..Statistically significant gene expression levels were obtained that differed by an order of magnitude when two different electric field strength and duration conditions were employed...
  24. doi request reprint Transfection by electroporation
    Huntington Potter
    Byrd Alzheimer s Institute, University of South Florida College of Medicine, Tampa, Florida, USA
    Curr Protoc Mol Biol . 2010
    ..Protocols are described for the use of electroporation in vivo to perform gene therapy for cancer therapy and DNA vaccination. Also described are modifications for preparation and transfection of plant protoplasts...
  25. ncbi request reprint Electroporation as a method for the efficient in vivo delivery of therapeutic genes
    Kenneth E Ugen
    Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    DNA Cell Biol 22:753. 2003
  26. ncbi request reprint Functional analysis of the hepatic HMG-CoA reductase promoter by in vivo electroporation
    William R Lagor
    Department of Molecular Medicine, College of Medicine, University of South Florida, Tampa, FL 33612, USA
    Exp Biol Med (Maywood) 232:353-61. 2007
    ..We also conclude that in vivo electroporation is a convenient and powerful technique for the analysis of promoter elements in the livers of live animals...
  27. ncbi request reprint In vivo electroporation for gene therapy
    Loree C Heller
    Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, FL 33612, USA
    Hum Gene Ther 17:890-7. 2006
  28. doi request reprint Subnanosecond electric pulses cause membrane permeabilization and cell death
    Shu Xiao
    Frank Reidy Research Center for Bioelectrics, Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, USA
    IEEE Trans Biomed Eng 58:1239-45. 2011
    ..This means that the cell membrane behaves like a rectifying diode. This increase in membrane conductance is a nonthermal process, since the temperature rise due to the pulsing is negligible...
  29. pmc Generation of a tumor spheroid in a microgravity environment as a 3D model of melanoma
    BERNADETTE MARRERO
    Department of Molecular Medicine, University of South Florida, Tampa, FL 33612, USA
    In Vitro Cell Dev Biol Anim 45:523-34. 2009
    ..The goal of engineering a 3D tissue model is to collect new information about cancer development and develop new potential treatment regimens that can be translated to in vivo models while reducing the use of laboratory animals...
  30. pmc Intradermal delivery of plasmid VEGF(165) by electroporation promotes wound healing
    Bernadette Ferraro
    Department of Molecular Medicine, University of South Florida, Tampa, Florida, USA
    Mol Ther 17:651-7. 2009
    ..Our results demonstrate that pVEGFE+ is a potential nonviral noninvasive therapy to increase perfusion and healing of skin flaps and ischemic wounds...
  31. doi request reprint Transfection by electroporation
    Huntington Potter
    Byrd Alzheimer s Institute, University of South Florida College of Medicine, Tampa, Florida, USA
    Curr Protoc Neurosci . 2011
    ..Protocols are described for the use of electroporation in vivo to perform gene therapy for cancer therapy and DNA vaccination. Also described are modifications for preparation and transfection of plant protoplasts...
  32. ncbi request reprint Assessment of delivery parameters with the multi-electrode array for development of a DNA vaccine against Bacillus anthracis
    Amy Donate
    University of South Florida, College of Medicine, 12901 Bruce B Downs Blvd, Tampa, FL 33612, USA Old Dominion University, Center for Bioelectrics, 4211 Monarch Way, Suite 300, Norfolk, VA 23508, USA Electronic address
    Bioelectrochemistry 94:1-6. 2013
    ..These results reflect the importance of establishing appropriate electrical parameters and the potential for the MEA in non-invasive DNA vaccination against B. anthracis...
  33. ncbi request reprint Electric field mediated DNA motion model
    Joseph D Hickey
    Department of Chemical Engineering, University of South Florida, 4202 E Fowler Ave ENB 118, Tampa, FL 33620, USA
    Bioelectrochemistry 70:101-3. 2007
    ..The resultant error comparing the simulation with experimental distance was 7.76%. Through a 1-D optimization procedure, this error was reduced to 3.02% for a 52 step simulation...
  34. ncbi request reprint Constitutive Stat3 activity up-regulates VEGF expression and tumor angiogenesis
    Guilian Niu
    Immunology Program, H Lee Moffitt Cancer Center and Research Institute, Department of Oncology, University of South Florida College of Medicine, Tampa, Florida, FL 33612, USA
    Oncogene 21:2000-8. 2002
    ..These studies provide evidence that the VEGF gene is regulated directly by Stat3 protein, and indicate that Stat3 represents a common molecular target for blocking angiogenesis induced by multiple signaling pathways in human cancers...

Research Grants9

  1. ELECTROCHEMOTHERAPY FOR TREATING SOFT TISSUE SARCOMAS
    Richard Heller; Fiscal Year: 2002
    ..An important consideration in the treatment of soft tissue sarcoma is to be able to perform limb sparing procedures which maintain functionality. ECT combined with surgery could fill this role. ..
  2. Therapeutic Potential of IL-15 Plasmid Delivery to Tumors Using Electroporation
    Richard Heller; Fiscal Year: 2010
    ..This study will examine if this strategy will work by utilizing a plasmid coding for IL-15 delivered directly into tumor cells. ..
  3. ELECTROCHEMOTHERAPY FOR TREATING SOFT TISSUE SARCOMAS
    Richard Heller; Fiscal Year: 1999
    ..An important consideration in the treatment of soft tissue sarcoma is to be able to perform limb sparing procedures which maintain functionality. ECT combined with surgery could fill this role. ..
  4. ELECTROCHEMOTHERAPY FOR TREATING SOFT TISSUE SARCOMAS
    Richard Heller; Fiscal Year: 2000
    ..An important consideration in the treatment of soft tissue sarcoma is to be able to perform limb sparing procedures which maintain functionality. ECT combined with surgery could fill this role. ..
  5. ELECTROCHEMOTHERAPY FOR TREATING SOFT TISSUE SARCOMAS
    Richard Heller; Fiscal Year: 2001
    ..An important consideration in the treatment of soft tissue sarcoma is to be able to perform limb sparing procedures which maintain functionality. ECT combined with surgery could fill this role. ..
  6. Electroporation System for Cutaneous Gene Transfer
    Richard Heller; Fiscal Year: 2009
    ..This information will help establish an effective way to deliver naked DNA to the skin. The investigators have pioneered the use of electroporation for in vivo delivery so are well suited to successfully complete the study. ..
  7. Therapeutic Potential of IL-15 Plasmid Delivery to Tumors Using Electroporation
    Richard Heller; Fiscal Year: 2009
    ..This study will examine if this strategy will work by utilizing a plasmid coding for IL-15 delivered directly into tumor cells. ..