Stephen J Beebe

Summary

Affiliation: Old Dominion University
Country: USA

Publications

  1. pmc An apoptosis targeted stimulus with nanosecond pulsed electric fields (nsPEFs) in E4 squamous cell carcinoma
    Wei Ren
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, 4211 Monarch Way, Norfolk, VA 23508, USA
    Apoptosis 16:382-93. 2011
  2. pmc Transient features in nanosecond pulsed electric fields differentially modulate mitochondria and viability
    Stephen J Beebe
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, United States of America
    PLoS ONE 7:e51349. 2012
  3. doi request reprint Nanosecond pulsed electric field ablation of hepatocellular carcinoma
    Stephen J Beebe
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
    Conf Proc IEEE Eng Med Biol Soc 2011:6861-5. 2011
  4. doi request reprint Non-ionizing radiation with nanosecond pulsed electric fields as a cancer treatment: in vitro studies
    Stephen J Beebe
    Eastern Virginia Medical School and Frank Reidy Research Center for Bioelectrics, Old Dominion University, USA
    Conf Proc IEEE Eng Med Biol Soc 2009:6509-12. 2009
  5. ncbi request reprint Long term survival of mice with hepatocellular carcinoma after pulse power ablation with nanosecond pulsed electric fields
    X Chen
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk Virginia, 4211 Monarch Way, Norfolk, Virginia 23508, USA
    Technol Cancer Res Treat 11:83-93. 2012
  6. ncbi request reprint Nanosecond pulsed electric fields modulate cell function through intracellular signal transduction mechanisms
    Stephen J Beebe
    Center for Pediatric Research, Eastern Virginia Medical School, Children s Hospital for The King s Daughters, Norfolk, VA, USA
    Physiol Meas 25:1077-93. 2004
  7. ncbi request reprint Nanosecond pulsed electric fields (nsPEF) induce direct electric field effects and biological effects on human colon carcinoma cells
    Emily H Hall
    Center for Pediatric Research, Eastern Virginia Medical School, Children s Hospital of the King s Daughters, Norfolk, Virginia 23510, USA
    DNA Cell Biol 24:283-91. 2005
  8. doi request reprint Nanosecond pulse electric field (nanopulse): a novel non-ligand agonist for platelet activation
    Jue Zhang
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA
    Arch Biochem Biophys 471:240-8. 2008
  9. ncbi request reprint Nanosecond electric pulses penetrate the nucleus and enhance speckle formation
    Nianyong Chen
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA
    Biochem Biophys Res Commun 364:220-5. 2007
  10. doi request reprint Nanosecond pulsed electric fields stimulate apoptosis without release of pro-apoptotic factors from mitochondria in B16f10 melanoma
    Wentia E Ford
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
    Arch Biochem Biophys 497:82-9. 2010

Collaborators

Detail Information

Publications26

  1. pmc An apoptosis targeted stimulus with nanosecond pulsed electric fields (nsPEFs) in E4 squamous cell carcinoma
    Wei Ren
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, 4211 Monarch Way, Norfolk, VA 23508, USA
    Apoptosis 16:382-93. 2011
    ..Nanosecond second pulsed electric fields trigger heterogeneous cell death mechanisms in E4 SCC populations to delete them, with caspase-associated cell death as a predominant, but not an unaccompanied event...
  2. pmc Transient features in nanosecond pulsed electric fields differentially modulate mitochondria and viability
    Stephen J Beebe
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, United States of America
    PLoS ONE 7:e51349. 2012
    ....
  3. doi request reprint Nanosecond pulsed electric field ablation of hepatocellular carcinoma
    Stephen J Beebe
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
    Conf Proc IEEE Eng Med Biol Soc 2011:6861-5. 2011
    ..This study demonstrates that nsPEF ablation is not limited to effectively treating skin cancers and provides a rationale for treating orthotopic hepatocellular carcinoma in pre-clinical applications and ultimately in clinical trials...
  4. doi request reprint Non-ionizing radiation with nanosecond pulsed electric fields as a cancer treatment: in vitro studies
    Stephen J Beebe
    Eastern Virginia Medical School and Frank Reidy Research Center for Bioelectrics, Old Dominion University, USA
    Conf Proc IEEE Eng Med Biol Soc 2009:6509-12. 2009
    ..Further, these in vitro studies suggest a potential to induce cell death that bypasses cancer mechanisms that evade apoptosis...
  5. ncbi request reprint Long term survival of mice with hepatocellular carcinoma after pulse power ablation with nanosecond pulsed electric fields
    X Chen
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk Virginia, 4211 Monarch Way, Norfolk, Virginia 23508, USA
    Technol Cancer Res Treat 11:83-93. 2012
    ..Based on present treatments for specific HCC stages, it is anticipated that nsPEFs could be substituted for or used in combination with ablation therapies using heat, cold or chemicals...
  6. ncbi request reprint Nanosecond pulsed electric fields modulate cell function through intracellular signal transduction mechanisms
    Stephen J Beebe
    Center for Pediatric Research, Eastern Virginia Medical School, Children s Hospital for The King s Daughters, Norfolk, VA, USA
    Physiol Meas 25:1077-93. 2004
    ....
  7. ncbi request reprint Nanosecond pulsed electric fields (nsPEF) induce direct electric field effects and biological effects on human colon carcinoma cells
    Emily H Hall
    Center for Pediatric Research, Eastern Virginia Medical School, Children s Hospital of the King s Daughters, Norfolk, Virginia 23510, USA
    DNA Cell Biol 24:283-91. 2005
    ..NsPEF applications provide a means to explore intracellular structures and functions that can reveal mechanisms in health and disease...
  8. doi request reprint Nanosecond pulse electric field (nanopulse): a novel non-ligand agonist for platelet activation
    Jue Zhang
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA
    Arch Biochem Biophys 471:240-8. 2008
    ..We conclude that nsPEFs dose-dependently cause platelets to rapidly aggregate, like other platelet agonists, and this is most likely initiated by the nsPEFs increasing [Ca2+]i, however by a different mechanism...
  9. ncbi request reprint Nanosecond electric pulses penetrate the nucleus and enhance speckle formation
    Nianyong Chen
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA
    Biochem Biophys Res Commun 364:220-5. 2007
    ..The resulting nuclear speckle changes were also cell cycle dependent. These findings suggest that 10ns pulses directly influenced nuclear processes, such as the changes in the nuclear RNA-protein complexes...
  10. doi request reprint Nanosecond pulsed electric fields stimulate apoptosis without release of pro-apoptotic factors from mitochondria in B16f10 melanoma
    Wentia E Ford
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA
    Arch Biochem Biophys 497:82-9. 2010
    ..nsPEFs provide a new cancer therapy that can bypass cancer-associated deregulation of mitochondria-mediated apoptosis in B16f10 melanoma...
  11. pmc Nanosecond pulsed electric fields cause melanomas to self-destruct
    Richard Nuccitelli
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA
    Biochem Biophys Res Commun 343:351-60. 2006
    ..Each pulse deposits 0.2 J and 100 pulses increase the temperature of the treated region by only 3 degrees C, ten degrees lower than the minimum temperature for hyperthermia effects...
  12. ncbi request reprint Nanosecond pulsed electric fields have differential effects on cells in the S-phase
    Emily H Hall
    Center for Pediatric Research, Eastern Virginia Medical School, Children s Hospital of the King s Daughters, Norfolk, Virginia 23510, USA
    DNA Cell Biol 26:160-71. 2007
    ..Furthermore, nsPEF effects on the nucleus and cytoskeleton may provide synergistic therapeutic actions with other agents, such as ionizing radiation or chemotherapeutics that affect these same structures...
  13. ncbi request reprint 3,4-Methylenedioxymethamphetamine activates nuclear factor-kappaB, increases intracellular calcium, and modulates gene transcription in rat heart cells
    David A Tiangco
    Department of Biological Sciences, Old Dominion University, Norfolk, VA 23529, USA
    Cardiovasc Toxicol 5:301-10. 2005
    ..Collectively, these results suggest that MDMA may be toxic to the heart through its ability to activate the myocardial NF-kappaB response, disrupt cytosolic calcium and mitochondrial homeostasis, and alter gene transcription...
  14. ncbi request reprint Diverse effects of nanosecond pulsed electric fields on cells and tissues
    Stephen J Beebe
    Eastern Virginia Medical School, Norfolk, Virginia 23510, USA
    DNA Cell Biol 22:785-96. 2003
    ..Potential applications for nsPEF include inducing apoptosis in cells and tumors, probing signal transduction mechanisms that determine cell fate, and enhancing gene expression...
  15. doi request reprint Cellular apoptosis by nanosecond, high-intensity electric pulses: model evaluation of the pulsing threshold and extrinsic pathway
    Jiahui Song
    Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529 0246, USA
    Bioelectrochemistry 79:179-86. 2010
    ..Finally, our results suggest that multi-needle electrode systems with adjustable field orientations would likely enhance apoptosis in the context of pulsed voltage-induced inactivation of tumor cells...
  16. ncbi request reprint Nanosecond pulsed electric fields induce apoptosis in p53-wildtype and p53-null HCT116 colon carcinoma cells
    Emily H Hall
    Center for Pediatric Research, Children s Hospital of the King s Daughters, Department of Physiological Sciences, Eastern Virginia Medical School, PO Box 1980, Norfolk, VA 23501 1980, USA
    Apoptosis 12:1721-31. 2007
    ....
  17. ncbi request reprint Nanosecond, high-intensity pulsed electric fields induce apoptosis in human cells
    Stephen J Beebe
    Center for Pediatric Research, 855 W Brambleton Ave, Norfolk, VA 23510, USA
    FASEB J 17:1493-5. 2003
    ..NsPEF technology provides a unique, high-power, energy-independent tool to recruit plasma membrane and/or intracellular signaling mechanisms that can delete aberrant cells by apoptosis...
  18. doi request reprint Nanosecond pulsed electric fields (nsPEFs) activate intrinsic caspase-dependent and caspase-independent cell death in Jurkat cells
    Wei Ren
    Frank Reidy Research Center for Bioelectric, Old Dominion University, Norfolk, VA 23508, United States
    Biochem Biophys Res Commun 421:808-12. 2012
    ..Furthermore, the results establish that nsPEFs can overcome natural and oncogenic mechanisms that promote cell survival through inhibition of apoptosis and other cell death mechanisms...
  19. ncbi request reprint Leukemic cell intracellular responses to nanosecond electric fields
    Nianyong Chen
    Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA
    Biochem Biophys Res Commun 317:421-7. 2004
    ..The results suggest application of this ultrashort pulse technology to modulate nuclear structure and function for potential therapeutic benefit...
  20. pmc A new pulsed electric field therapy for melanoma disrupts the tumor's blood supply and causes complete remission without recurrence
    Richard Nuccitelli
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA
    Int J Cancer 125:438-45. 2009
    ..This new physical tumor therapy is drug free, highly localized, uses low energy, has no significant side effects and results in very little scarring...
  21. doi request reprint Apoptosis initiation and angiogenesis inhibition: melanoma targets for nanosecond pulsed electric fields
    Xinhua Chen
    Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA
    Pigment Cell Melanoma Res 23:554-63. 2010
    ..The nsPEFs activate multiple melanoma therapeutic targets, which is consistent with successes of nsPEF applications for tumor treatment in vivo as a new cancer therapeutic modality...
  22. ncbi request reprint Stimulation of capacitative calcium entry in HL-60 cells by nanosecond pulsed electric fields
    Jody A White
    Biomedical Sciences Program, Old Dominion University and Eastern Virginia Medical School, Norfolk, Virginia, USA
    J Biol Chem 279:22964-72. 2004
    ..These findings suggest that nsPEFs, like ligand-mediated responses, release calcium from similar internal calcium pools and thus activate plasma membrane calcium influx channels or capacitative calcium entry...
  23. pmc The effects of intense submicrosecond electrical pulses on cells
    Jingdong Deng
    Physical Electronics Research Institute, Old Dominion University, Norfolk, Virginia 23529, USA
    Biophys J 84:2709-14. 2003
    ....
  24. ncbi request reprint Resveratrol induces apoptosis in LNCaP cells and requires hydroxyl groups to decrease viability in LNCaP and DU 145 cells
    Gary Z Morris
    Department of Chemistry and Biochemistry, Old Dominion University, Norfolk, Virginia, USA
    Prostate 52:319-29. 2002
    ..This study was conducted to determine the effects of resveratrol on prostate cancer cell viability through apoptosis induction and the significance of the three hydroxyl groups on resveratrol to the measured effect...
  25. ncbi request reprint Cocaine, not morphine, causes the generation of reactive oxygen species and activation of NF-kappaB in transiently cotransfected heart cells
    Barbara Y Hargrave
    Department of Biological Sciences, Old Dominion University, Norfolk, VA 23529, USA
    Cardiovasc Toxicol 3:141-51. 2003
    ..The results suggest that cocaine induces free radical production, which leads to the activation of NF-kappaB signal transduction and possible inflammatory responses...