Chang-An Yu

Summary

Affiliation: Oklahoma State University
Country: USA

Publications

  1. ncbi request reprint Crystallization of proteins from soluble cytochrome P-450cam to membrane protein cytochrome bc(1) complex
    Chang An Yu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochem Biophys Res Commun 312:191-5. 2003
  2. ncbi request reprint Inter- and intra-molecular electron transfer in the cytochrome bc(1) complex
    Chang An Yu
    Department of Biochemistry and Molecular Biology, NRC 255, OAES, Oklahoma State University, Stillwater, OK74078, USA
    Biochim Biophys Acta 1555:65-70. 2002
  3. doi request reprint Domain conformational switch of the iron-sulfur protein in cytochrome bc1 complex is induced by the electron transfer from cytochrome bL to bH
    Chang An Yu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochim Biophys Acta 1777:1038-43. 2008
  4. pmc Formation of engineered intersubunit disulfide bond in cytochrome bc1 complex disrupts electron transfer activity in the complex
    He Wen Ma
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochim Biophys Acta 1777:317-26. 2008
  5. ncbi request reprint The iron-sulfur cluster of the Rieske iron-sulfur protein functions as a proton-exiting gate in the cytochrome bc(1) complex
    Buddha Gurung
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 280:24895-902. 2005
  6. ncbi request reprint Evidence for electron equilibrium between the two hemes bL in the dimeric cytochrome bc1 complex
    Xing Gong
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 280:9251-7. 2005
  7. ncbi request reprint The role of extra fragment at the C-terminal of cytochrome b (Residues 421-445) in the cytochrome bc1 complex from Rhodobacter sphaeroides
    Xiaoying Liu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 279:47363-71. 2004
  8. pmc Simultaneous reduction of iron-sulfur protein and cytochrome b(L) during ubiquinol oxidation in cytochrome bc(1) complex
    Jian Zhu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Proc Natl Acad Sci U S A 104:4864-9. 2007
  9. ncbi request reprint Identification of amino acid residues essential for reconstitutive activity of subunit IV of the cytochrome bc1 complex from Rhodobacter sphaeroides
    Shih Chia Tso
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochim Biophys Acta 1757:1561-7. 2006
  10. ncbi request reprint The disulfide bridge in the head domain of rhodobacter sphaeroides cytochrome c1 is needed to maintain its structural integrity
    Maria Elberry
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    Biochemistry 45:4991-7. 2006

Research Grants

Collaborators

Detail Information

Publications30

  1. ncbi request reprint Crystallization of proteins from soluble cytochrome P-450cam to membrane protein cytochrome bc(1) complex
    Chang An Yu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochem Biophys Res Commun 312:191-5. 2003
  2. ncbi request reprint Inter- and intra-molecular electron transfer in the cytochrome bc(1) complex
    Chang An Yu
    Department of Biochemistry and Molecular Biology, NRC 255, OAES, Oklahoma State University, Stillwater, OK74078, USA
    Biochim Biophys Acta 1555:65-70. 2002
    ....
  3. doi request reprint Domain conformational switch of the iron-sulfur protein in cytochrome bc1 complex is induced by the electron transfer from cytochrome bL to bH
    Chang An Yu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochim Biophys Acta 1777:1038-43. 2008
    ....
  4. pmc Formation of engineered intersubunit disulfide bond in cytochrome bc1 complex disrupts electron transfer activity in the complex
    He Wen Ma
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochim Biophys Acta 1777:317-26. 2008
    ....
  5. ncbi request reprint The iron-sulfur cluster of the Rieske iron-sulfur protein functions as a proton-exiting gate in the cytochrome bc(1) complex
    Buddha Gurung
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 280:24895-902. 2005
    ..It was speculated that in the normal catalytic cycle of the bc(1) complex, the [2Fe-2S] cluster may function as a proton-exiting gate...
  6. ncbi request reprint Evidence for electron equilibrium between the two hemes bL in the dimeric cytochrome bc1 complex
    Xing Gong
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 280:9251-7. 2005
    ..This supports the idea that the interruption of electron transfer between the two bL hemes enhances electron leakage to oxygen and thus decreases the ubiquinol-cytochrome c reductase activity...
  7. ncbi request reprint The role of extra fragment at the C-terminal of cytochrome b (Residues 421-445) in the cytochrome bc1 complex from Rhodobacter sphaeroides
    Xiaoying Liu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 279:47363-71. 2004
    ..These results indicate that the first 12 residues (residues 421-432) from the N-terminal end of the C-terminal extra fragment of cytochrome b are essential for maintaining structural integrity of the bc1 complex...
  8. pmc Simultaneous reduction of iron-sulfur protein and cytochrome b(L) during ubiquinol oxidation in cytochrome bc(1) complex
    Jian Zhu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Proc Natl Acad Sci U S A 104:4864-9. 2007
    ..Under the same conditions, by using stopped flow, the reduction rates of cytochromes b(H) and c(1) were 403 s(-1) (t(1/2) 1.7 ms) and 164 s(-1) (t(1/2) 4.2 ms), respectively...
  9. ncbi request reprint Identification of amino acid residues essential for reconstitutive activity of subunit IV of the cytochrome bc1 complex from Rhodobacter sphaeroides
    Shih Chia Tso
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochim Biophys Acta 1757:1561-7. 2006
    ..However, these interactions are not essential for the incorporation of subunit IV into the bc(1) complex...
  10. ncbi request reprint The disulfide bridge in the head domain of rhodobacter sphaeroides cytochrome c1 is needed to maintain its structural integrity
    Maria Elberry
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    Biochemistry 45:4991-7. 2006
    ..The disulfide bridge is essential in maintaining the structural integrity of cytochrome c(1) and thus the functionality of the cytochrome bc(1) complex...
  11. ncbi request reprint Generation, characterization and crystallization of a highly active and stable cytochrome bc1 complex mutant from Rhodobacter sphaeroides
    Maria Elberry
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, 74078, USA
    Biochim Biophys Acta 1757:835-40. 2006
    ..3 degrees C increase in the thermo-denaturation temperature. Crystals formed from this mutant complex, in the presence of stigmatellin, diffract X-rays up to 2.9 Angstroms resolution...
  12. pmc Interaction between cytochrome caa3 and F1F0-ATP synthase of alkaliphilic Bacillus pseudofirmus OF4 is demonstrated by saturation transfer electron paramagnetic resonance and differential scanning calorimetry assays
    Xiaoying Liu
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    Biochemistry 46:306-13. 2007
    ..pseudofirmus OF4 in a reconstituted system. They support the suggestion that such an interaction between these complexes may contribute to sequestered proton transfers during alkaliphile oxidative phosphorylation at high pH...
  13. ncbi request reprint The role of an extra fragment of cytochrome b (residues 309-326) in the cytochrome bc1 complex from Rhodobacter sphaeroides
    Xing Gong
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    Biochemistry 45:11122-9. 2006
    ..This change facilitates electron leakage from the Q(o) site for reaction with molecular oxygen to form superoxide anion, thus decreasing bc(1) activity...
  14. doi request reprint Domain movement of iron sulfur protein in cytochrome bc1 complex is facilitated by the electron transfer from cytochrome b(L) to b(H)
    Xiaowei Cen
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    FEBS Lett 582:523-6. 2008
    ..Pm inhibitor has a higher affinity and better efficacy with the cytochrome b(H) reduced complex and Pf binds better and has a higher efficacy with the ISP reduced complex...
  15. ncbi request reprint Effect of famoxadone on photoinduced electron transfer between the iron-sulfur center and cytochrome c1 in the cytochrome bc1 complex
    Kunhong Xiao
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 278:11419-26. 2003
    ..The effects of famoxadone binding on electron transfer were also studied in a series of Rhodobacter sphaeroides cyt bc(1) mutants involving residues at the interface between the Rieske protein and cyt c(1) and/or cyt b...
  16. ncbi request reprint The ubiquinone-binding site in NADH:ubiquinone oxidoreductase from Escherichia coli
    Xing Gong
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 278:25731-7. 2003
    ..Using the PHDhtm hydropathy plot, the labeled peptide is located in the transmembrane helix 4 toward the periplasmic side of the membrane...
  17. ncbi request reprint The extra fragment of the iron-sulfur protein (residues 96-107) of Rhodobacter sphaeroides cytochrome bc1 complex is required for protein stability
    Kunhong Xiao
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    Biochemistry 43:1488-95. 2004
    ..The order of essentiality of residues in this fragment is residues 104-107 > residues 100-103 > residues 96-99...
  18. ncbi request reprint Nonoxidizable ubiquinol derivatives that are suitable for the study of the ubiquinol oxidation site in the cytochrome bc1 complex
    Li Zhang
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
    Biochim Biophys Acta 1556:226-32. 2002
    ..The binding site of TMDMP does not overlap with the binding sites of stigmatellin, MOA-stilbene (MOAS), undecylhydroxydioxobenzothiazole (UHDBT) and myxothaizol...
  19. ncbi request reprint Identification of the ubiquinone-binding domain in the disulfide catalyst disulfide bond protein B
    Tong Xie
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 277:1649-52. 2002
    ..This sequence occurs in the second periplasmic domain of the inner membrane protein DsbB in a loop connecting transmembrane helices 3 and 4. We propose that the quinone-binding site is within or very near to this sequence...
  20. pmc Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 103:13045-50. 2006
    ..A mechanism for the high fidelity of the bifurcated electron transfer is proposed...
  21. ncbi request reprint Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4255, USA
    J Mol Biol 341:281-302. 2004
    ..Class P contains two subgroups, Pm and Pf, that are distinct by their ability to induce mobile or fixed conformation of iron-sulfur protein...
  22. ncbi request reprint Structural basis for the quinone reduction in the bc1 complex: a comparative analysis of crystal structures of mitochondrial cytochrome bc1 with bound substrate and inhibitors at the Qi site
    Xiugong Gao
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 42:9067-80. 2003
    ..A two-water-mediated ubiquinone protonation mechanism is proposed involving three Q(i) site residues His(201), Lys(227), and Asp(228)...
  23. pmc Effect of mutations in the cytochrome b ef loop on the electron-transfer reactions of the Rieske iron-sulfur protein in the cytochrome bc1 complex
    Sany Rajagukguk
    Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, USA
    Biochemistry 46:1791-8. 2007
    ..Studies of all of the mutants provide further evidence that the ef loop plays an important role in regulating the domain movement of the ISP to facilitate productive electron transfer and prevent short-circuit reactions...
  24. ncbi request reprint Design of a ruthenium-labeled cytochrome c derivative to study electron transfer with the cytochrome bc1 complex
    Gregory Engstrom
    Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, USA
    Biochemistry 42:2816-24. 2003
    ..The interaction of yeast Ru-39-Cc with yeast cytochrome bc1 is stronger than that of horse Ru-39-Cc with bovine cytochrome bc1, suggesting that nonpolar interactions are stronger in the yeast system...
  25. ncbi request reprint Photoinduced electron transfer between the Rieske iron-sulfur protein and cytochrome c(1) in the Rhodobacter sphaeroides cytochrome bc(1) complex. Effects of pH, temperature, and driving force
    Gregory Engstrom
    Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR 72701, USA
    J Biol Chem 277:31072-8. 2002
    ..It is concluded that the electron transfer reaction from 2Fe-2S to cyt c(1) is controlled by conformational gating...
  26. ncbi request reprint Inhibitor-complexed structures of the cytochrome bc1 from the photosynthetic bacterium Rhodobacter sphaeroides
    Lothar Esser
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:2846-57. 2008
    ..Functional implications for the bc(1) complex are derived from analyses of 10 independent molecules in various crystal forms and from comparisons with mitochondrial complexes...
  27. ncbi request reprint Structural basis for the mechanism of electron bifurcation at the quinol oxidation site of the cytochrome bc1 complex
    Di Xia
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, NIH, 37 Convent Dr, Building 37, Room 2122C, Bethesda, MD 20892, USA
    Photosynth Res 92:17-34. 2007
    ....
  28. pmc Identification of a ubiquinone-binding site that affects autophosphorylation of the sensor kinase RegB
    Lee R Swem
    Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
    J Biol Chem 281:6768-75. 2006
    ....
  29. pmc Disulfide bond formation involves a quinhydrone-type charge-transfer complex
    James Regeimbal
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 1048, USA
    Proc Natl Acad Sci U S A 100:13779-84. 2003
    ..We propose a simple model for the action of DsbB where a quinhydrone-like complex plays a crucial role as a reaction intermediate...
  30. pmc Functional genetic screening reveals the role of mitochondrial cytochrome b as a mediator of FAS-induced apoptosis
    Andrei P Komarov
    Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Proc Natl Acad Sci U S A 105:14453-8. 2008
    ..These results reveal a cytochrome c-independent branch of FAS-induced apoptosis involving cleavage and cytoplasmic release of mitochondrial Cyt b...

Research Grants2

  1. REACTION MECHANISM OF MYOCARDIAC UBIQUINONE-PROTEINS
    Chang An Yu; Fiscal Year: 2007
    ..For type specifications, see instructions on page 6.) RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..