G J Nuovo

Summary

Country: USA

Publications

  1. ncbi Rapid diagnosis of smallpox infection and differentiation from its mimics
    Gerard J Nuovo
    Department of Pathology, The Ohio State University Medical Center S305E Rhodes Hall, 450 W 10th Avenue Columbus, OH 43210, USA
    Diagn Mol Pathol 12:103-7. 2003
  2. ncbi Histologic, infectious, and molecular correlates of idiopathic spontaneous abortion and perinatal mortality
    Gerard J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus, OH 43210, USA
    Diagn Mol Pathol 14:152-8. 2005
  3. ncbi The surgical and cytopathology of viral infections: utility of immunohistochemistry, in situ hybridization, and in situ polymerase chain reaction amplification
    Gerard J Nuovo
    Department of Pathology, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Ann Diagn Pathol 10:117-31. 2006
  4. ncbi The utility of in situ--based methodologies including in situ polymerase chain reaction for the diagnosis and study of viral infections
    Gerard J Nuovo
    Department of Pathology Ohio State University Medical Center, Columbus, OH 43210, USA
    Hum Pathol 38:1123-36. 2007
  5. ncbi In situ detection of precursor and mature microRNAs in paraffin embedded, formalin fixed tissues and cell preparations
    Gerard J Nuovo
    Department of Pathology and the Comprehensive Cancer Center, Ohio State University Medical Center, 81 HLRI, 473 W 12th Avenue, Columbus, OH 43210, USA
    Methods 44:39-46. 2008
  6. pmc Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway
    Katie Twigger
    Division of Cancer Biology Chester Beatty Laboratories, The Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK
    BMC Cancer 12:368. 2012
  7. doi Reovirus-associated reduction of microRNA-let-7d is related to the increased apoptotic death of cancer cells in clinical samples
    Gerard J Nuovo
    The Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA
    Mod Pathol 25:1333-44. 2012
  8. doi In situ detection of microRNAs in paraffin embedded, formalin fixed tissues and the co-localization of their putative targets
    Gerard J Nuovo
    The Comprehensive Cancer Center, Ohio State University Medical Center, Columbus, OH, USA
    Methods 52:307-15. 2010
  9. pmc Benign metastasizing leiomyoma of the lung: clinicopathologic, immunohistochemical, and micro-RNA analyses
    Gerard J Nuovo
    Department of Pathology, The Ohio State University Medical Center, Room 081 Heart and Lung Research Institute, 473 W 12th Avenue, Columbus, OH 43210, USA
    Diagn Mol Pathol 17:145-50. 2008
  10. ncbi Histologic distribution of fatal rotaviral pneumonitis: an immunohistochemical and RT in situ PCR analysis
    Gerard J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus 43210, USA
    Diagn Mol Pathol 11:140-5. 2002

Collaborators

Detail Information

Publications103 found, 100 shown here

  1. ncbi Rapid diagnosis of smallpox infection and differentiation from its mimics
    Gerard J Nuovo
    Department of Pathology, The Ohio State University Medical Center S305E Rhodes Hall, 450 W 10th Avenue Columbus, OH 43210, USA
    Diagn Mol Pathol 12:103-7. 2003
    ..Formalin fixation, which is optimal for in situ hybridization, guarantees the inactivation of the smallpox virus...
  2. ncbi Histologic, infectious, and molecular correlates of idiopathic spontaneous abortion and perinatal mortality
    Gerard J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus, OH 43210, USA
    Diagn Mol Pathol 14:152-8. 2005
    ..It is concluded that in utero infection and the associated cytokine up-regulation are responsible for many cases of unexplained fetal and neonatal loss...
  3. ncbi The surgical and cytopathology of viral infections: utility of immunohistochemistry, in situ hybridization, and in situ polymerase chain reaction amplification
    Gerard J Nuovo
    Department of Pathology, The Ohio State University Medical Center, Columbus, OH 43210, USA
    Ann Diagn Pathol 10:117-31. 2006
    ..A combination of routine histopathology, clinical information, and immunohistochemistry/in situ-based nucleic acid detection methodologies will allow the surgical pathologist to correctly diagnose viral infections...
  4. ncbi The utility of in situ--based methodologies including in situ polymerase chain reaction for the diagnosis and study of viral infections
    Gerard J Nuovo
    Department of Pathology Ohio State University Medical Center, Columbus, OH 43210, USA
    Hum Pathol 38:1123-36. 2007
    ..By combining this information with, for example, cytokine localization, one can elucidate much about the pathophysiology of the viral infection that cannot be afforded by polymerase chain reaction-based methods alone...
  5. ncbi In situ detection of precursor and mature microRNAs in paraffin embedded, formalin fixed tissues and cell preparations
    Gerard J Nuovo
    Department of Pathology and the Comprehensive Cancer Center, Ohio State University Medical Center, 81 HLRI, 473 W 12th Avenue, Columbus, OH 43210, USA
    Methods 44:39-46. 2008
    ..Further, one can easily determine the specific subcellular compartmentalization of the precursor and mature forms which may provide insight into the modulation of these important regulatory molecules and their targets...
  6. pmc Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway
    Katie Twigger
    Division of Cancer Biology Chester Beatty Laboratories, The Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK
    BMC Cancer 12:368. 2012
    ..In particular, we analysed the role of EGFR/Ras signalling in determining virus-mediated cytotoxicity in SCCHN...
  7. doi Reovirus-associated reduction of microRNA-let-7d is related to the increased apoptotic death of cancer cells in clinical samples
    Gerard J Nuovo
    The Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA
    Mod Pathol 25:1333-44. 2012
    ..A rate-limiting factor of reovirus-induced cancer cell death is productive viral infection that operates via the marked reduction of microRNA-let-7d and concomitant elevated caspase-3 expression...
  8. doi In situ detection of microRNAs in paraffin embedded, formalin fixed tissues and the co-localization of their putative targets
    Gerard J Nuovo
    The Comprehensive Cancer Center, Ohio State University Medical Center, Columbus, OH, USA
    Methods 52:307-15. 2010
    ..Co-expression of a microRNA and its putative target in tissue sections offers physiologic corroboration of solution-based methods that a given microRNA may be regulating a specific protein...
  9. pmc Benign metastasizing leiomyoma of the lung: clinicopathologic, immunohistochemical, and micro-RNA analyses
    Gerard J Nuovo
    Department of Pathology, The Ohio State University Medical Center, Room 081 Heart and Lung Research Institute, 473 W 12th Avenue, Columbus, OH 43210, USA
    Diagn Mol Pathol 17:145-50. 2008
    ..In conclusion, benign metastasizing leiomyomas are indeed most likely benign lesions, and up-regulation of miR-221 expression is an accurate way to differentiate leiomyosarcoma from benign metastasizing leiomyoma...
  10. ncbi Histologic distribution of fatal rotaviral pneumonitis: an immunohistochemical and RT in situ PCR analysis
    Gerard J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus 43210, USA
    Diagn Mol Pathol 11:140-5. 2002
    ..It is concluded that rotaviral infection can lead to fatal pneumonitis and that the mechanism of this complication is centered on a diffuse septal endothelialitis with concomitant tissue damage...
  11. doi Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection
    Gerard J Nuovo
    The Comprehensive Cancer Center, Rio de Janeiro, Brazil
    Diagn Mol Pathol 19:135-43. 2010
    ..HPV L2-induced inactivation of miR-125b is associated with the classic cytologic changes of the koilocyte, and the exogenous application of mimetic miR-125b markedly inhibits HPV DNA synthesis...
  12. pmc A methodology for the combined in situ analyses of the precursor and mature forms of microRNAs and correlation with their putative targets
    Gerard J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus, Ohio, USA
    Nat Protoc 4:107-15. 2009
    ..Each miRNA in situ method takes 1 d. The final step of the protocol involves colabeling by immunohistochemistry for the putative target of the miRNA, which is done after the in situ hybridization step and takes a few hours...
  13. ncbi Co-labeling using in situ PCR: a review
    G J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus, Ohio 43210, USA
    J Histochem Cytochem 49:1329-39. 2001
    ..Radioactive 3H-labeled nucleotides permit an excellent distinction with colorimetric signals for co-localization, although two distinct chromogens can in many instances allow successful localization of two different targets...
  14. ncbi The utility of immunohistochemistry and in situ hybridization in placental pathology
    Gerard Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus 43210, USA
    Arch Pathol Lab Med 130:979-83. 2006
    ....
  15. pmc In situ detection of mature microRNAs by labeled extension on ultramer templates
    Gerard Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus, OH 43210, USA
    Biotechniques 46:115-26. 2009
    ....
  16. ncbi The histologic spectrum of epidermodysplasia verruciformis
    G J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus 43210, USA
    Am J Surg Pathol 24:1400-6. 2000
    ....
  17. ncbi The role of human papillomavirus in gynecological diseases
    G J Nuovo
    Dept of Pathology, Ohio State University Medical Center, Columbus 43210, USA
    Crit Rev Clin Lab Sci 37:183-215. 2000
    ..This review highlights the role of HPV in the evolution of disease in the lower genital tract and stresses the utility of HPV testing to the diagnostic pathologist...
  18. ncbi In situ localization of PCR-amplified DNA and cDNA
    G J Nuovo
    MGN Medical Consultants, Inc, Setauket, NY 11733, USA
    Mol Biotechnol 10:49-62. 1998
    ....
  19. ncbi Hypermethylation of the MLH1 promoter with concomitant absence of transcript and protein occurs in small patches of crypt cells in unaffected mucosa from sporadic colorectal carcinoma
    Gerard J Nuovo
    Department of Pathology, Comprehensive Cancer Center, Ohio State University Medical Center, Columbus, OH 43210, USA
    Diagn Mol Pathol 15:17-23. 2006
    ..A combination of methylation-specific in situ PCR with in situ RNA and protein analysis can assist in documenting rare foci of promoter hypermethylation-induced gene silencing...
  20. doi Role of oxidative tissue injury in the pathophysiology of experimentally induced equine laminitis: a comparison of 2 models
    T A Burns
    Ohio State University College of Veterinary Medicine, Columbus, OH 43210, USA
    J Vet Intern Med 25:540-8. 2011
    ..The role of oxidative stress in the CHO model remains unknown...
  21. ncbi Highly differentiated keratinizing squamous cell cancer of the cervix: a rare, locally aggressive tumor not associated with human papillomavirus or squamous intraepithelial lesions
    C Morrison
    Department of Pathology, Ohio State University Medical Center, Columbus 43210, USA
    Am J Surg Pathol 25:1310-5. 2001
    ....
  22. ncbi Age-related hypermethylation of the 5' region of MLH1 in normal colonic mucosa is associated with microsatellite-unstable colorectal cancer development
    H Nakagawa
    Division of Human Cancer Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio 43210, USA
    Cancer Res 61:6991-5. 2001
    ..We conclude that the spread of methylation in the MLH1 promoter in the normal colonic mucosa is closely associated with age and the development of sporadic MSI+ colorectal cancers...
  23. ncbi Immune factors involved in the cervical immune response in the HIV/HPV co-infection
    A F Nicol
    Laboratory of Immunopathology, Instituto Oswaldo Cruz DBBM, FIOCRUZ, Rio de Janeiro, Brazil
    J Clin Pathol 61:84-8. 2008
    ..This study investigates the expression of RANTES, MIP1alpha, COX1, COX2, STAT3, TGFbetaRI, IL10R, TNFalphaRII and TLR4 in the cervical immune response in HIV/HPV (human papillomavirus) co-infected women...
  24. ncbi In situ detection of SOCS and cytokine expression in the uterine cervix from HIV/HPV coinfected women
    A F Nicol
    Immunology Service of Evandro Chagas Clinical Research Institute, IPEC, FIOCRUZ, Avenue Brazil 4365 Manguinhos, Rio de Janeiro, CEP 21045 900, Brazil
    Exp Mol Pathol 81:42-7. 2006
    ....
  25. ncbi Histologic distribution of fatal rotaviral infection: an immunohistochemical and reverse transcriptase in situ polymerase chain reaction analysis
    C Morrison
    Department of Pathology, Ohio State University Medical Center, Columbus, OH 43210-1228, USA
    Hum Pathol 32:216-21. 2001
    ..It is concluded that rotaviral infection can lead to a fatal disseminated infection in humans and the mechanism of this complication is based on a diffuse endothelialitis and concomitant tissue damage...
  26. ncbi Utility of the in situ detection of HPV in Pap smears diagnosed as within normal limits
    G Menezes
    Department of Pathology, Ohio State University Medical Center, Columbus 43210, USA
    Acta Cytol 45:919-26. 2001
    ..In situ hybridization is very effective in detecting rare, atypical cells in Pap smears diagnosed as within normal limits and, in a high-risk population, is predictive of SIL on clinical follow-up...
  27. ncbi Preparation of samples for polymerase chain reaction in situ
    G J Nuovo
    MGN Medical Research Laboratories, Setauket, NY 11733, USA
    Scanning Microsc Suppl 10:49-55. 1996
    ..Otherwise, one needs to use a labeled probe and a hybridization step to detect amplified DNA targets in paraffin embedded tissues...
  28. ncbi In situ determination of T-cell receptor beta expression patterns
    G J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus, Ohio 43210-1228, USA
    J Histochem Cytochem 49:139-45. 2001
    ..We conclude that the RT in situ PCR methodology will allow the routine determination of monoclonal vs multiclonal expression patterns of the TCR beta using archival paraffin-embedded tissues.(J Histochem Cytochem 49:139-145, 2001)..
  29. ncbi In situ determination of B-cell heavy chain and kappa/lambda light chain expression patterns: methodology and clinical utility
    C Morrison
    Department of Pathology, Ohio State University School of Medicine and Public Health, Columbus, USA
    Diagn Mol Pathol 10:171-8. 2001
    ....
  30. doi Presence of mononuclear cells in normal and affected laminae from the black walnut extract model of laminitis
    R R Faleiros
    Department of Veterinary Clinical Sciences, Ohio State University, OH, USA
    Equine Vet J 43:45-53. 2011
    ..There is increasing evidence of involvement of inflammatory cells in acute laminitis...
  31. ncbi Diagnosis of human papillomavirus using in situ hybridization and in situ polymerase chain reaction
    G J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus, OH, USA
    Methods Mol Biol 179:113-36. 2002
  32. doi Calprotectin in myeloid and epithelial cells of laminae from horses with black walnut extract-induced laminitis
    R R Faleiros
    Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA
    J Vet Intern Med 23:174-81. 2009
    ..Laminar inflammation is one of the earliest events in equine laminitis. Calprotectin (CP), a Damage-Associated Molecular Pattern protein, is overexpressed in inflammatory conditions of human skin...
  33. doi Laminar leukocyte accumulation in horses with carbohydrate overload-induced laminitis
    R R Faleiros
    Department of Veterinary Clinical Sciences, Ohio State University, Columbus, OH 43210, USA
    J Vet Intern Med 25:107-15. 2011
    ..While there is evidence of laminar leukocyte infiltration in black walnut extract (BWE)-induced laminitis, there is no such evidence for carbohydrate overload (CHO) laminitis...
  34. ncbi The histologic spectrum of epidermodysplasia verruciformis in transplant and AIDS patients
    Carl Morrison
    Department of Pathology, Ohio State University Medical Center, Columbus, 43210, USA
    J Cutan Pathol 29:480-9. 2002
    ..The purpose of this study was to correlate the histologic findings of skin lesions clinically suspicious for epidermodysplasia verruciformis with the viral findings in patients with organ transplants or AIDS...
  35. pmc In situ detection of the hypermethylation-induced inactivation of the p16 gene as an early event in oncogenesis
    G J Nuovo
    MGN Medical Research Laboratory, Setauket, NY 11733, USA
    Proc Natl Acad Sci U S A 96:12754-9. 1999
    ..These studies demonstrate that MSP-ISH is a powerful approach for studying the dynamics of aberrant methylation of critical tumor suppressor genes during tumor evolution...
  36. ncbi Increased incidence of atypical Papanicolaou tests from ThinPreps of postmenopausal women receiving hormone replacement therapy
    G A Menezes
    Department of Pathology, Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Cancer 93:357-63. 2001
    ....
  37. ncbi Coxsackie virus infection of the placenta associated with neurodevelopmental delays in the newborn
    E Euscher
    Department of Pathology, Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Obstet Gynecol 98:1019-26. 2001
    ..This underscores the importance of detailed pathologic and viral examination of the placenta in cases of systemic illness in the newborn...
  38. ncbi The utility of the in situ detection of T-cell receptor Beta rearrangements in cutaneous T-cell-dominant infiltrates
    Cynthia M Magro
    Department of Pathology, Ohio State University, Columbus, OH, USA
    Diagn Mol Pathol 12:133-41. 2003
    ..In conclusion, RT in situ PCR for TCRbeta, which can be done on formalin-fixed biopsies and allows direct correlation of the molecular data with the histology, is a useful adjunctive test in the differentiation of CTCL from its mimics...
  39. doi Correlation of Pap smear, cervical biopsy, and clinical follow-up with an HPV typing microarray system
    Gerard J Nuovo
    Department of Pathology, Ohio State University Medical Center, OH, USA
    Diagn Mol Pathol 17:107-11. 2008
    ..It is concluded that the MyGene Assay allows for the routine typing of HPV in liquid-based Pap smears, and that the presence of HPV DNA in ASCUS Pap smears is strongly correlated with a biopsy-proven SIL...
  40. ncbi Detection of HIV-1 provirus and RNA by in situ amplification
    Alcina Nicol
    Immunology Service of Evandro Chagas Research Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
    Methods Mol Biol 304:171-82. 2005
    ..This chapter will describe the methodologies for detecting HIV-1 DNA and RNA in paraffin-embedded tissue sections as well as the colabeling experiments needed to define the host response to the viral invasion...
  41. ncbi Utility of HPV analysis for evaluation of possible metastatic disease in women with cervical cancer
    Jose Antonio Plaza
    Department of Pathology, Ohio State University Medical Center, Columbus, 43210, USA
    Int J Gynecol Pathol 23:7-12. 2004
    ..Reverse transcriptase in situ polymerase chain reaction for human papillomavirus RNA is a reliable method to differentiate metastatic cervical carcinoma from either a new primary tumor or a metastasis from another cancer...
  42. ncbi Oral contraceptive pills are associated with artifacts in ThinPrep Pap smears that mimic low-grade squamous intraepithelial lesions
    Carl Morrison
    Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Cancer 99:75-82. 2003
    ..02). The purpose of this study was to correlate these morphologic changes with human papillomavirus (HPV) DNA detection by in situ hybridization and clinical follow-up...
  43. ncbi Histologic correlates of viral and bacterial infection of the placenta associated with severe morbidity and mortality in the newborn
    Anjali Satosar
    Department of Pathology, Ohio State University Medical Center, Columbus, OH 43210, USA
    Hum Pathol 35:536-45. 2004
    ..025). These data show that infection of the villi is highly associated with neonatal morbidity and mortality and that the histologic findings are, in most cases, nonspecific for infection...
  44. ncbi The histologic differentiation of oral condyloma acuminatum from its mimics
    K Mark Anderson
    Department of Oral Pathology, Ohio State University Medical Center, Columbus 43210, USA
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 96:420-8. 2003
    ..The purpose of this study was to determine which histologic features could enable one to distinguish oral condyloma, as defined by the detection of human papillomavirus (HPV) DNA through in situ hybridization, from its mimics...
  45. ncbi Correlation of histology, viral load, and in situ viral detection in hepatic biopsies from patients with liver transplants secondary to hepatitis C infection
    Gerard J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus, OH 43210, USA
    Hum Pathol 33:277-84. 2002
    ..01). We conclude that certain histologic features and an increased viral load are highly correlated with the in situ detection of viral RNA in the liver, which is consistent with recurrent viral infection...
  46. ncbi Longterm efficacy of interferon-alpha for extrahepatic disease associated with hepatitis C virus infection
    M Naarendorp
    Department of Pathology, Ohio State University School of Medicine, Columbus, USA
    J Rheumatol 28:2466-73. 2001
    ..Careful monitoring is needed for evolution of liver pathology to cirrhosis, or for progression of renal or neurologic disease...
  47. pmc Modulation of mismatch repair and genomic stability by miR-155
    Nicola Valeri
    Department of Molecular Virology, Immunology and Medical Genetics, Ohio State University Medical Center, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 107:6982-7. 2010
    ..Finally, a number of MSI tumors with unknown cause of MMR inactivation displayed miR-155 overexpression. These data provide support for miR-155 modulation of MMR as a mechanism of cancer pathogenesis...
  48. ncbi Verruca vulgaris of the vulva in children and adults: a nonvenereal type of vulvar wart
    Irene Aguilera-Barrantes
    Department of Pathology, The Ohio State University Medical Center, Columbus, OH, USA
    Am J Surg Pathol 31:529-35. 2007
    ..This highlights the value of HPV testing in such cases, especially if the histologic changes are consistent with verruca vulgaris...
  49. ncbi Histologic and in situ viral findings in the myocardium in cases of sudden, unexpected death
    Adina M Cioc
    Department of Pathology, Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Mod Pathol 15:914-22. 2002
    ..Although Coxsackie virus was, as expected, the most common virus noted, other viruses including rotavirus and HIV-1 were also observed, highlighting the need for comprehensive viral and histologic analyses in such cases...
  50. ncbi Correlation of histology, human papillomavirus, and viral load in laryngeal papillomas of childhood
    Girishwar Sharma
    Department of Pathology, Ohio State University Medical Center, Columbus, OH 43210, USA
    Diagn Mol Pathol 14:230-6. 2005
    ....
  51. ncbi Parvoviral infection of endothelial cells and its possible role in vasculitis and autoimmune diseases
    Cynthia M Magro
    Department of Pathology, Ohio State University, Columbus 43210, USA
    J Rheumatol 29:1227-35. 2002
    ..To analyze a series of biopsies from 16 patients who, on the basis of clinical and dermatopathologic findings, had a spectrum of connective tissue diseases (CTD), autoinflammatory or CTD-like syndromes for parvoviral DNA, RNA, and protein...
  52. ncbi Distribution of immune cell subsets and cytokine-producing cells in the uterine cervix of human papillomavirus (HPV)-infected women: influence of HIV-1 coinfection
    Alcina Frederica Nicol
    Immunology Service, Chagas Research Institute, Oswaldo Cruz Foundation, Avenida Brasil, Rio de Janeiro, RJ, Brazil
    Diagn Mol Pathol 14:39-47. 2005
    ....
  53. ncbi Methylation-specific PCR in situ hybridization
    Gerard J Nuovo
    Department of Pathology, Ohio State University Medical Center, Columbus, USA
    Methods Mol Biol 287:261-72. 2004
    ..Furthermore, since one often uses an oligoprobe, probe size (at least 40 bp), labeling method (3' end tailing), probe concentration, and posthybridization stringency conditions can all have an impact on the final results...
  54. pmc Cell-cycle and suppressor proteins expression in uterine cervix in HIV/HPV co-infection: comparative study by tissue micro-array (TMA)
    Alcina F Nicol
    Laboratory of Immunopathology, Instituto Oswaldo Cruz FIOCRUZ, Rio de Janeiro, Brazil
    BMC Cancer 8:289. 2008
    ..The oncoproteins of human papillomavirus (HPVs) directly effect cell-cycle control. We hypothesize that regulatory and cell cycle protein expression might be additionally modified in the cervix of HIV/HPV co-infected women...
  55. doi SOCS in situ expression in tuberculous lymphadenitis in an endemic area
    A F Nicol
    Laboratory of Immunopathology, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil
    Exp Mol Pathol 84:240-4. 2008
    ..The immune response to Mycobacterium tuberculosis is complex and multifactorial, the cytokine system being a major factor in M. tuberculosis immunity...
  56. pmc MicroRNA 133B targets pro-survival molecules MCL-1 and BCL2L2 in lung cancer
    Melissa Crawford
    Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, DHLRI, 473 West 12th Avenue, Room 201, Columbus, OH 43210, USA
    Biochem Biophys Res Commun 388:483-9. 2009
    ..To our knowledge, this represents the first observation of decreased expression of miR-133B in lung cancer and that it functionally targets members of the BCL-2 family...
  57. pmc MicroRNA expression in response to murine myocardial infarction: miR-21 regulates fibroblast metalloprotease-2 via phosphatase and tensin homologue
    Sashwati Roy
    Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, 473 West 12th Avenue, Columbus, OH 43210, USA
    Cardiovasc Res 82:21-9. 2009
    ..For the first time, we sought to test how myocardial ischaemia-reperfusion (IR) changes miR expression...
  58. pmc Systematic evaluation of microRNA processing patterns in tissues, cell lines, and tumors
    Eun Joo Lee
    College of Pharmacy, Ohio State University, Columbus, Ohio 43210, USA
    RNA 14:35-42. 2008
    ..We provide a database of the levels of precursor and mature miRNA in a variety of cell types. Our data demonstrate that a large number of miRNAs are transcribed but are not processed to the mature miRNA...
  59. pmc Head and neck squamous cell carcinomas in HIV-positive patients: a preliminary investigation of viral associations
    Michael S McLemore
    Department of Pathology, MD Anderson, Houston, TX, USA
    Head Neck Pathol 4:97-105. 2010
    ..MNTCG are significantly common in HIV+ HNSCC, but there is overlap in MNTCG counts with the control group and therefore this finding cannot be used as a biomarker of HIV infection...
  60. ncbi Histologic, viral, and molecular correlates of dengue fever infection of the liver using highly sensitive immunohistochemistry
    Fabiane Carvalho de Macedo
    Department of Pathology Antônio Pedro University Hospital Federal Fluminense university, Niteroi, Rio de Janeiro, Brazil
    Diagn Mol Pathol 15:223-8. 2006
    ..The infection can be readily detected in a few hours using an automated system that has a sensitivity equivalent to reverse transcriptase in situ polymerase chain reaction...
  61. ncbi Parvovirus B19 associated adult Henoch Schönlein purpura
    Adina M Cioc
    Department of Pathology, Ohio State University, Columbus, OH 43210, USA
    J Cutan Pathol 29:602-7. 2002
    ..However, direct localization of the viral DNA to the glomerular and cutaneous endothelium in HSP in correlation with the histopathologic findings has not been demonstrated...
  62. ncbi Molecular detection of rabies encephalitis and correlation with cytokine expression
    Gerard J Nuovo
    1Department of Pathology, The Ohio State University, Columbus, OH, USA
    Mod Pathol 18:62-7. 2005
    ....
  63. ncbi Detection of kappa- and lambda-expressing cells in the endometrium by in situ hybridization
    Elizabeth Euscher
    Department of Pathology, Ohio State University Medical Center, Columbus 43210, USA
    Int J Gynecol Pathol 21:383-90. 2002
    ..In situ hybridization for light chain expression can be helpful in endometria where only very rare plasma cells are seen; a baseline result would rule out chronic endometritis...
  64. ncbi Correlation of HIV-1 detection and histology in AIDS-associated emphysema
    Martha M Yearsley
    Department of Pathology, The Ohio State University, Columbus, Ohio 43210 1228, USA
    Diagn Mol Pathol 14:48-52. 2005
    ..These results suggest that AIDS-related emphysema may be due, in part, to direct infection by HIV-1 of, primarily, alveolar macrophages, and concomitant up-regulation of MMP expression in the neighboring, noninfected cells...
  65. ncbi Oral plasmablastic lymphomas in AIDS patients are associated with human herpesvirus 8
    Adina M Cioc
    Department of Pathology, Ohio State University Medical Center and College of Dentistry, Columbus, OH 43210, USA
    Am J Surg Pathol 28:41-6. 2004
    ..Given the poor prognosis of oral cavity lymphomas in immunocompromised patients, therapy directed against the HHV8 and EBV infection may be of therapeutic value...
  66. ncbi Primary effusion lymphoma: cytopathologic diagnosis using in situ molecular genetic analysis for human herpesvirus 8
    Paul E Wakely
    Department of Pathology, The Ohio State University, Columbus, Ohio 43210, USA
    Mod Pathol 15:944-50. 2002
    ..Definitive diagnosis of primary effusion lymphoma is possible directly from cytologic smears/cell block by combining cytologic morphology with reverse transcriptase in situ polymerase chain reaction detection of human herpesvirus-8...
  67. ncbi Histologic and molecular correlates of fatal measles infection in children
    Jose Antonio Plaza
    Department of Pathology, The Ohio State University, Columbus, Ohio 43210 1228, USA
    Diagn Mol Pathol 14:97-102. 2005
    ....
  68. pmc In vivo reduction or blockade of interleukin-1β in primary osteoarthritis influences expression of mediators implicated in pathogenesis
    K S Santangelo
    Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA
    Osteoarthritis Cartilage 20:1610-8. 2012
    ..Diminish interleukin-1β (IL-1β) signaling in a model of primary osteoarthritis by RNA interference-based transcript reduction or receptor blockade, and quantify changes incurred on transcript expression of additional mediators...
  69. doi In vivo and in vitro evidence of the involvement of CXCL1, a keratinocyte-derived chemokine, in equine laminitis
    R R Faleiros
    Department of Veterinary Clinical Sciences, Ohio State University, Columbus, OH 43210, USA
    J Vet Intern Med 23:1086-96. 2009
    ..C-X-C motif ligand 1 (CXCL1) is an important chemokine of epithelial origin in rodents and humans...
  70. ncbi Microsphere-adenoviral complexes target and transduce the glomerulus in vivo
    N S Nahman
    Department of Internal Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    Kidney Int 58:1500-10. 2000
    ..We postulated that adenoviral-microsphere complexes could result in DNA transfer (transduction) into glomerular cells in vivo...
  71. doi A summary of the 25th International Papillomavirus Conference 2009: vaccines, screening, epidemiology and therapeutics
    Alcina F Nicol
    Instituto Oswaldo Cruz FIOCRUZ, Av Brasil 4365, Rio de Janeiro, Brazil
    J Clin Virol 47:208-15. 2010
    ..All the references cited are from the abstract book of the IPV Conference. See http://www.hpv2009.org/...
  72. ncbi Eosinophilic intranuclear inclusion bodies in a melanocytic nevus
    Geoffrey K Hahm
    Department of Pathology Ohio State University, Columbus, USA
    Cutis 69:223-6. 2002
    ..This result suggests that such inclusions may be related to molluscum viral infection of melanocytes...
  73. pmc MicroRNAs in the pathogenesis of Lung Cancer
    Xin Wu
    The Ohio State University Medical Center, Division of Hematology and Oncology, Columbus, Ohio 43210, USA
    J Thorac Oncol 4:1028-34. 2009
    ..Furthermore, investigators are beginning to identify individual targets and pathways of miRNAs relevant to lung tumorigenesis. Thus, miRNAs may identify critical targets and be important in the pathogenesis of lung cancer...
  74. ncbi EBV kidney allograft infection: possible relationship with a peri-graft localization of PTLD
    Fernando G Cosio
    Internal Medicine Pathology Surgery, The Ohio State University, Columbus, Ohio, USA
    Am J Transplant 4:116-23. 2004
    ..It is concluded that EBV can infect kidney allografts, and there appears to be a relationship between this infection and the presence of PTLD near the graft...
  75. ncbi Epm2a suppresses tumor growth in an immunocompromised host by inhibiting Wnt signaling
    Yin Wang
    Division of Cancer Immunology, Department of Pathology, and Comprehensive Cancer Center, Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Cancer Cell 10:179-90. 2006
    ..Epm2a-encoded laforin is a phosphatase for GSK-3beta and an important repressor in the Wnt signaling pathway. Inactivation of Epm2a resulted in increased Wnt signaling and tumorigenesis...
  76. ncbi Automated kappa and lambda light chain mRNA expression for the assessment of B-cell clonality in cutaneous B-cell infiltrates: its utility and diagnostic application
    Cynthia Magro
    Department of Pathology, Division of Hematology Oncology, Ohio State University School of Medicine and Public Health, Columbus, OH, USA
    J Cutan Pathol 30:504-11. 2003
    ..We sought to assess the utility of kappa (kappa) and lambda (lambda) mRNA expression using the Ventana automated assay (Ventana Medical Systems, Tucson, AZ, USA) in the analysis of atypical cutaneous B-cell lymphoid infiltrates...
  77. ncbi Expression of mu-BCR-ADL transcripts in chronic neutrophilic leukemia
    Adina M Cioc
    Department of Pathology, Ohio State University Medical Center, Columbus 43210, USA
    Am J Clin Pathol 118:842-7. 2002
    ..To our knowledge, this is thefirst in situ demonstration of mu-BCR-ABL expression in CNL Ourfindings reinforce the usefulness of this messenger RNA as a molecular marker of CNL...
  78. pmc Chromosome 21-derived microRNAs provide an etiological basis for aberrant protein expression in human Down syndrome brains
    Donald E Kuhn
    College of Pharmacy, Division of Pharmacology, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 285:1529-43. 2010
    ..Finally these results suggest that selective inactivation of Hsa21-derived miRNAs may provide a novel therapeutic tool in the treatment of DS...
  79. pmc miR-802 regulates human angiotensin II type 1 receptor expression in intestinal epithelial C2BBe1 cells
    Sarah E Sansom
    Davis Heart and Lung Research Institute, Department of Pathology, The Ohio State Univ, Columbus, 43210, USA
    Am J Physiol Gastrointest Liver Physiol 299:G632-42. 2010
    ..These results suggest that miR-802 can modulate the expression of the hAT(1)R in the GI tract and ultimately play a role in regulating the biological efficacy of Ang II in this system...
  80. pmc Methylation mediated silencing of MicroRNA-1 gene and its role in hepatocellular carcinogenesis
    Jharna Datta
    Department of Molecular and Cellular Biochemistry, College of Pharmacy, and Comprehensive Cancer Center, Ohio State University, Columbus, Ohio 43210, USA
    Cancer Res 68:5049-58. 2008
    ..The inhibition of cell cycle progression and induction of apoptosis after re-expression of miR-1 are some of the mechanisms by which DNA hypomethylating agents suppress hepatocarcinoma cell growth...
  81. ncbi PCR-amplified vitronectin mRNA localizes in situ to spermatocytes and round spermatids in the human testis
    G J Nuovo
    Department of Pathology, State University of New York, Stony Brook 11794 8091, USA
    Hum Reprod 10:2187-91. 1995
    ..This demonstrates that the translated protein present in the spermatozoon is being produced in situ. Further study is needed to determine the role of this protein in the dynamics of sperm-oocyte interaction...
  82. pmc Integrating the MicroRNome into the study of lung disease
    SERGE P NANA-SINKAM
    Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Ohio State University Medical Center, Columbus, OH 43210, USA
    Am J Respir Crit Care Med 179:4-10. 2009
    ....
  83. ncbi C-erb B2 (Her2/neu) is neither overexpressed nor amplified in hepatic neoplasms
    Dmitry M Vlasoff
    Department of Pathology, Ohio State University, Columbus, Ohio 43210 1228, USA
    Appl Immunohistochem Mol Morphol 10:237-41. 2002
    ..C-erb B2 amplification was not demonstrated in hepatocellular carcinomas. Neither overexpression nor amplification of c-erb B2 (Her2/ ) can be regarded as a useful prognostic factor in hepatocellular carcinoma...
  84. ncbi Diagnostic microRNA markers for screening sporadic human colon cancer and active ulcerative colitis in stool and tissue
    Farid E Ahmed
    GEM Tox Consultants and Labs, Inc, 2607 Calvin Way, Greenville, NC 27834, USA
    Cancer Genomics Proteomics 6:281-95. 2009
    ....
  85. ncbi Determination of light chain restriction in fine-needle aspiration-type preparations of B-cell lymphomas by mRNA in situ hybridization
    Lawrence H Lee
    Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
    Appl Immunohistochem Mol Morphol 12:252-8. 2004
    ..In situ kappa/lambda mRNA analysis of FNA-type specimens allows direct determination of monoclonality in cytologic preparations...
  86. pmc Human chromosome 21-derived miRNAs are overexpressed in down syndrome brains and hearts
    Donald E Kuhn
    Davis Heart and Lung Research Institute, The Ohio State University, DHLRI 515, 473 West 12th Avenue, Columbus, OH 43210, USA
    Biochem Biophys Res Commun 370:473-7. 2008
    ..Importantly, Hsa21-derived miRNAs may provide novel therapeutic targets in the treatment of individuals with DS...
  87. ncbi Experimental treatment of Epstein-Barr virus-associated primary central nervous system lymphoma
    Sameek Roychowdhury
    Department of Molecular Virology, Immunology, Medical Genetics, The Ohio State University, Columbus, Ohio, 43210, USA
    Cancer Res 63:965-71. 2003
    ..These results suggest that antiviral therapies can be effectively explored in vivo using a preclinical animal model of human EBV(+) PCNSL with subsequent translation to patients with EBV(+) PCNSL...
  88. pmc Granulocyte macrophage colony-stimulating factor inhibits breast cancer growth and metastasis by invoking an anti-angiogenic program in tumor-educated macrophages
    Tim D Eubank
    Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Internal Medicine, The Dorothy M Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA
    Cancer Res 69:2133-40. 2009
    ..In situ hybridization and flow cytometry identified macrophages as the primary source of sVEGFR-1. These data suggest that GM-CSF can re-educate macrophages to reduce angiogenesis and metastases in murine breast cancer...
  89. ncbi The acute respiratory distress syndrome: a role for transforming growth factor-beta 1
    Ruairi J Fahy
    Division of Pulmonary and Critical Care Medicine, Dorothy M Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 1252, USA
    Am J Respir Cell Mol Biol 28:499-503. 2003
    ..These data may lead to new therapeutic approaches in ARDS through more targeted inhibition of fibrosis...
  90. ncbi Human papillomavirus 16 and 18 expression in conjunctival intraepithelial neoplasia
    Ingrid U Scott
    Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, FL 33101, USA
    Ophthalmology 109:542-7. 2002
    ..To evaluate conjunctival intraepithelial neoplasia (CIN) and normal conjunctiva for the presence of human papillomavirus (HPV) DNA and for expression (as detected by the presence of mRNA) of the HPV E6 region...
  91. ncbi Correlation of in situ detection of infectious agents in the placenta with neonatal outcome
    Linda Genen
    Department of Pediatrics, Winthrop University Hospital, SUNY Stony Brook School of Medicine, Mineola, New York 11501, USA
    J Pediatr 144:316-20. 2004
    ..To determine if infections involving the placenta are associated with unexplained systemic illness in the newborn infant and subsequent poor neonatal outcome (death or significant neurodevelopmental abnormalities)...
  92. ncbi A human CD34(+) subset resides in lymph nodes and differentiates into CD56bright natural killer cells
    Aharon G Freud
    Department of Molecular Virology, Immunology, and Medical Genetics, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio 43210, USA
    Immunity 22:295-304. 2005
    ..The data identify a new NK precursor and support a model of human NK development in which BM-derived CD34dimCD45RA(+)beta7bright HPCs reside in LN where endogenous cytokines drive their differentiation to CD56bright NK cells in vivo...
  93. ncbi Cellular localization and function of Fas ligand (CD95L) in tumors
    Masayuki Kurooka
    Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 62:1261-5. 2002
    ..Adoptive transfer into genetically deficient mice revealed that host CD95-CD95L function did not alter tumor growth, demonstrating that CD95L alone does not affect tumor growth...
  94. pmc Thy-1 promoter hypermethylation: a novel epigenetic pathogenic mechanism in pulmonary fibrosis
    Yan Y Sanders
    Department of Pediatrics, Division of Pulmonary Medicine, University of Alabama at Birmingham, Birmingham, AL 35233, USA
    Am J Respir Cell Mol Biol 39:610-8. 2008
    ..Epigenetic regulation of Thy-1 is a novel and potentially reversible mechanism in fibrosis that may offer the possibility of new therapeutic options...
  95. ncbi The absence of CD20 messenger RNA in recurrent cutaneous B-cell lymphoma following rituximab therapy
    Yeshwant B Rawal
    Department of Oral and Maxillofacial Pathology, The Ohio State University, Athens, 43210, USA
    J Cutan Pathol 32:616-21. 2005
    ..Single-agent rituximab therapy is safe and well tolerated. Recurrences showing a loss of CD20 expression following rituximab therapy have been reported...
  96. pmc Expression profiling identifies microRNA signature in pancreatic cancer
    Eun Joo Lee
    College of Pharmacy, Ohio State University, Columbus, OH 43210, USA
    Int J Cancer 120:1046-54. 2007
    ..Aberrant microRNA expression may offer new clues to pancreatic tumorigenesis and may provide diagnostic biomarkers for pancreatic adenocarcinoma...
  97. ncbi Cutaneous manifestations of Crohn's disease, its spectrum, and its pathogenesis: intracellular consensus bacterial 16S rRNA is associated with the gastrointestinal but not the cutaneous manifestations of Crohn's disease
    A Neil Crowson
    Central Medical Laboratories, Winnipeg, Manitoba, Canada
    Hum Pathol 34:1185-92. 2003
    ..Bacteria do, however, appear to play a role in bowel lesions of patients with CD...
  98. ncbi Epstein-Barr virus- and human herpesvirus 8-associated primary cutaneous plasmablastic lymphoma in the setting of renal transplantation
    Shannon Verma
    College of Medicine and Public Health, The Ohio State University, OH 43210 1218, USA
    J Cutan Pathol 32:35-9. 2005
    ..We describe a case of PBL occurring in the setting of renal transplantation...
  99. ncbi Immunohistochemical overexpression of p16 and p53 in uterine serous carcinoma and ovarian high-grade serous carcinoma
    Andres G Chiesa-Vottero
    Department of Anatomic Pathology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Int J Gynecol Pathol 26:328-33. 2007
    ..The overexpression of p16 in USC is unrelated to HPV. Further studies are necessary to determine whether p16 expression is useful in the differential diagnosis of ovarian high-grade serous carcinoma...
  100. ncbi Methylation of p16(INK4a) promoters occurs in vivo in histologically normal human mammary epithelia
    Charles R Holst
    Department of Pathology and University of California at San Francisco Comprehensive Cancer Center 94143 0511, USA
    Cancer Res 63:1596-601. 2003
    ..Here we show that epithelial cells with methylation of p16 promoter sequences occur in focal patches of histologically normal mammary tissue of a substantial fraction of healthy, cancer-free women...
  101. ncbi Stiff-person syndrome: a rare presentation for breast cancer
    Ergun Kocak
    Breast J 10:552-3. 2004