G J Nuovo
Nuovo G. The surgical and cytopathology of viral infections: utility of immunohistochemistry, in situ hybridization, and in situ polymerase chain reaction amplification. Ann Diagn Pathol. 2006;10:117-31 pubmed
..A combination of routine histopathology, clinical information, and immunohistochemistry/in situ-based nucleic acid detection methodologies will allow the surgical pathologist to correctly diagnose viral infections. ..
Nuovo G, Schmittgen T. Benign metastasizing leiomyoma of the lung: clinicopathologic, immunohistochemical, and micro-RNA analyses. Diagn Mol Pathol. 2008;17:145-50 pubmed publisher
..In conclusion, benign metastasizing leiomyomas are indeed most likely benign lesions, and up-regulation of miR-221 expression is an accurate way to differentiate leiomyosarcoma from benign metastasizing leiomyoma. ..
Nuovo G, Wu X, Volinia S, Yan F, Di Leva G, Chin N, et al
. Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection. Diagn Mol Pathol. 2010;19:135-43 pubmed publisher
..HPV L2-induced inactivation of miR-125b is associated with the classic cytologic changes of the koilocyte, and the exogenous application of mimetic miR-125b markedly inhibits HPV DNA synthesis. ..
Nuovo G, Owor G, Andrew T, Magro C. Histologic distribution of fatal rotaviral pneumonitis: an immunohistochemical and RT in situ PCR analysis. Diagn Mol Pathol. 2002;11:140-5 pubmed
..It is concluded that rotaviral infection can lead to fatal pneumonitis and that the mechanism of this complication is centered on a diffuse septal endothelialitis with concomitant tissue damage. ..
Nuovo G, Lee E, Lawler S, Godlewski J, Schmittgen T. In situ detection of mature microRNAs by labeled extension on ultramer templates. Biotechniques. 2009;46:115-26 pubmed publisher
Nuovo G. In situ detection of microRNAs in paraffin embedded, formalin fixed tissues and the co-localization of their putative targets. Methods. 2010;52:307-15 pubmed publisher
..Co-expression of a microRNA and its putative target in tissue sections offers physiologic corroboration of solution-based methods that a given microRNA may be regulating a specific protein. ..
Nuovo G, Nakagawa H, Sotamaa K, Chapelle A. Hypermethylation of the MLH1 promoter with concomitant absence of transcript and protein occurs in small patches of crypt cells in unaffected mucosa from sporadic colorectal carcinoma. Diagn Mol Pathol. 2006;15:17-23 pubmed
..A combination of methylation-specific in situ PCR with in situ RNA and protein analysis can assist in documenting rare foci of promoter hypermethylation-induced gene silencing. ..