PATRICIA SPEAR

Summary

Affiliation: Northwestern University
Country: USA

Publications

  1. ncbi Different receptors binding to distinct interfaces on herpes simplex virus gD can trigger events leading to cell fusion and viral entry
    Patricia G Spear
    Department of Microbiology Immunology, MC S213, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611, USA
    Virology 344:17-24. 2006
  2. ncbi Herpes simplex virus: receptors and ligands for cell entry
    Patricia G Spear
    Feinberg School of Medicine of Northwestern University, Chicago, IL 60611, USA
    Cell Microbiol 6:401-10. 2004
  3. pmc Herpesvirus entry: an update
    Patricia G Spear
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 77:10179-85. 2003
  4. pmc Mutations in the N termini of herpes simplex virus type 1 and 2 gDs alter functional interactions with the entry/fusion receptors HVEM, nectin-2, and 3-O-sulfated heparan sulfate but not with nectin-1
    Miri Yoon
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 77:9221-31. 2003
  5. pmc Differential effects on cell fusion activity of mutations in herpes simplex virus 1 glycoprotein B (gB) dependent on whether a gD receptor or a gB receptor is overexpressed
    Qing Fan
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 83:7384-90. 2009
  6. ncbi Alanine substitution of conserved residues in the cytoplasmic tail of herpes simplex virus gB can enhance or abolish cell fusion activity and viral entry
    Nancy Ruel
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611, USA
    Virology 346:229-37. 2006
  7. pmc Use of herpes simplex virus and pseudorabies virus chimeric glycoprotein D molecules to identify regions critical for membrane fusion
    Anna Zago
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 101:17498-503. 2004
  8. ncbi Effects of linker-insertion mutations in herpes simplex virus 1 gD on glycoprotein-induced fusion with cells expressing HVEM or nectin-1
    Cheryl R Jogger
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Virology 318:318-26. 2004
  9. pmc Differences in the N termini of herpes simplex virus type 1 and 2 gDs that influence functional interactions with the human entry receptor Nectin-2 and an entry receptor expressed in Chinese hamster ovary cells
    Anna Zago
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 77:9695-9. 2003
  10. pmc Mutations in the N-terminal domains of nectin-1 and nectin-2 reveal differences in requirements for entry of various alphaherpesviruses and for nectin-nectin interactions
    Frank Struyf
    Department of Microbiology Immunology, The Feinberg School of Medicine, Northwestern University, 320 E Superior Street, Chicago, IL 60611, USA
    J Virol 76:12940-50. 2002

Research Grants

  1. HERPESVIRUS GENE EXPRESSION IN TRANSFORMED CELLS
    PATRICIA SPEAR; Fiscal Year: 2007
  2. ENTRY OF HERPES SIMPLEX VIRUS INTO CELLS
    PATRICIA SPEAR; Fiscal Year: 2007
  3. HERPESVIRUS GENE EXPRESSION IN TRANSFORMED CELLS
    PATRICIA SPEAR; Fiscal Year: 2005
  4. INTL CONG OF VIROLOGY, SAN FRANCISCO, ASV TRAVEL REQUEST
    PATRICIA SPEAR; Fiscal Year: 2005
  5. Herpes simplex virus receptors and signal transduction
    PATRICIA SPEAR; Fiscal Year: 2003
  6. HERPESVIRUS GENE EXPRESSION IN TRANSFORMED CELLS
    PATRICIA SPEAR; Fiscal Year: 2000

Collaborators

  • L Corey
  • Yasushi Kawaguchi
  • Kenneth M Murphy
  • LEWIS LANIER
  • JIAN NONE LIU
  • A C Steven
  • Hiroshi Nakato
  • Nicholas W Shworak
  • Nancy Ruel
  • Miri Yoon
  • Erick Lin
  • Anna Zago
  • Frank Struyf
  • Qing Fan
  • Cheryl R Jogger
  • Takeshi Satoh
  • Carl F Ware
  • Julia O Jackson
  • Sarah J Kopp
  • Hisashi Arase
  • John R Sedy
  • Wanda M Martinez
  • Joann M Taylor
  • Roger Lawrence
  • Derek D Sloan
  • Melissa McNeely
  • Timothy C Cheung
  • Liza Q Bundesen
  • Sharmila Manoj
  • Keisuke Kamimura
  • Deepak Shukla
  • Richard Longnecker
  • Ulrike E Maurer
  • Richard J Miller
  • Pavel Osten
  • Kelly Glajch
  • Ghazal Banisadr
  • Kay Grünewald
  • Amane Kogure
  • Shinya Tanaka
  • Jun Arii
  • Noriko Arase
  • Jing Wang
  • Ikuo Shiratori
  • Tadahiro Suenaga
  • Junji Uehori
  • Miroslaw Lech
  • Robert D Rosenberg
  • John Rhodes
  • Jun Miyoshi
  • Sassan HajMohammadi
  • Paul A Toselli
  • Klaus Pfeffer
  • Tomio Yabe
  • Yoshimi Takai
  • Nanette Susmarski
  • Edward D Lamperti
  • David C Johnson
  • Aaron J Hinz
  • Mary Stewart
  • Tracy K Sandifer
  • Keith R Jerome
  • Jin Young Han
  • Rochelle Jean-Jacques
  • Aileen E Plate
  • Nell S Lurain
  • Paula S Norris
  • Bonnie R Tran
  • Karen G Potter
  • Ian R Humphreys
  • Chris A Benedict
  • Ginelle Patterson
  • Heather M Shumway
  • Koji Kimata
  • John M Rhodes
  • Rebecca I Montgomery
  • Ryu Ueda
  • Dawn Myscofski
  • Christine M Posavad
  • Marc Van Ranst
  • Els Keyaerts

Detail Information

Publications30

  1. ncbi Different receptors binding to distinct interfaces on herpes simplex virus gD can trigger events leading to cell fusion and viral entry
    Patricia G Spear
    Department of Microbiology Immunology, MC S213, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611, USA
    Virology 344:17-24. 2006
    ..Finally, information and ideas are presented about a membrane-proximal region of gD that is required for membrane fusion, but not for receptor binding, and that may have a role in activating the fusogenic activity of gB, gH and gL...
  2. ncbi Herpes simplex virus: receptors and ligands for cell entry
    Patricia G Spear
    Feinberg School of Medicine of Northwestern University, Chicago, IL 60611, USA
    Cell Microbiol 6:401-10. 2004
    ....
  3. pmc Herpesvirus entry: an update
    Patricia G Spear
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 77:10179-85. 2003
  4. pmc Mutations in the N termini of herpes simplex virus type 1 and 2 gDs alter functional interactions with the entry/fusion receptors HVEM, nectin-2, and 3-O-sulfated heparan sulfate but not with nectin-1
    Miri Yoon
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 77:9221-31. 2003
    ..The sequence of the N terminus determines whether nectin-2 or 3-O-sulfated heparan sulfate, as well as HVEM, can serve as entry/fusion receptors...
  5. pmc Differential effects on cell fusion activity of mutations in herpes simplex virus 1 glycoprotein B (gB) dependent on whether a gD receptor or a gB receptor is overexpressed
    Qing Fan
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 83:7384-90. 2009
    ....
  6. ncbi Alanine substitution of conserved residues in the cytoplasmic tail of herpes simplex virus gB can enhance or abolish cell fusion activity and viral entry
    Nancy Ruel
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611, USA
    Virology 346:229-37. 2006
    ..Other mutations, particularly the deletion, significantly enhanced cell fusion activity. These mutations, as well as others described previously, identify regions of the gB cytoplasmic domain that modulate cell fusion activity...
  7. pmc Use of herpes simplex virus and pseudorabies virus chimeric glycoprotein D molecules to identify regions critical for membrane fusion
    Anna Zago
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 101:17498-503. 2004
    ....
  8. ncbi Effects of linker-insertion mutations in herpes simplex virus 1 gD on glycoprotein-induced fusion with cells expressing HVEM or nectin-1
    Cheryl R Jogger
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Virology 318:318-26. 2004
    ..These and other results indicate that the mutations disruptive of interactions with both receptors probably affect conformations of contact sites that are different for each receptor...
  9. pmc Differences in the N termini of herpes simplex virus type 1 and 2 gDs that influence functional interactions with the human entry receptor Nectin-2 and an entry receptor expressed in Chinese hamster ovary cells
    Anna Zago
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 77:9695-9. 2003
    ....
  10. pmc Mutations in the N-terminal domains of nectin-1 and nectin-2 reveal differences in requirements for entry of various alphaherpesviruses and for nectin-nectin interactions
    Frank Struyf
    Department of Microbiology Immunology, The Feinberg School of Medicine, Northwestern University, 320 E Superior Street, Chicago, IL 60611, USA
    J Virol 76:12940-50. 2002
    ..We can conclude that structural requirements for HSV entry, PRV and BHV-1 entry, and homotypic and heterotypic trans interactions are all different despite the previously reported ability of HSV and HSV gD to inhibit trans interactions...
  11. pmc Mutations in herpes simplex virus glycoprotein D that prevent cell entry via nectins and alter cell tropism
    Sharmila Manoj
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 101:12414-21. 2004
    ....
  12. pmc Random mutagenesis of the gene encoding a viral ligand for multiple cell entry receptors to obtain viral mutants altered for receptor usage
    Miri Yoon
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 101:17252-7. 2004
    ..These and other results show that features of gD, including conformation of the N terminus, critical for functional interactions with HVEM/3-O-sulfated heparan sulfate, differ from those critical for interactions with nectin-1...
  13. pmc Deletion of the second immunoglobulin-like domain of nectin-1 alters its intracellular processing and localization and ability to mediate entry of herpes simplex virus
    Frank Struyf
    Northwestern University, The Feinberg School of Medicine, Department of Microbiology and Immunology, Searle 6 447, 320 E Superior St, Chicago, IL 60611, USA
    J Virol 79:3841-5. 2005
    ..The failure of nectin-1-Delta2 to mediate HSV entry probably resulted from its failure to be transported to a membrane targeted by HSV for viral entry...
  14. pmc Random linker-insertion mutagenesis to identify functional domains of herpes simplex virus type 1 glycoprotein B
    Erick Lin
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, MC S213, 320 East Superior Street, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 104:13140-5. 2007
    ..Ten were between residues exposed to the surface of the trimer, identifying regions that may be critical for interactions with other viral proteins or cellular components or for transitions from the prefusion to postfusion state...
  15. pmc Alternative entry receptors for herpes simplex virus and their roles in disease
    Joann M Taylor
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Cell Host Microbe 2:19-28. 2007
    ..This study illustrates the value of mutant mice for understanding receptor contributions to disease caused by a human virus...
  16. pmc Insertional mutations in herpes simplex virus type 1 gL identify functional domains for association with gH and for membrane fusion
    Qing Fan
    Department of Microbiology Immunology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 83:11607-15. 2009
    ....
  17. ncbi Viral interactions with receptors in cell junctions and effects on junctional stability
    Patricia G Spear
    Department of Microbiology Immunology, The Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Dev Cell 3:462-4. 2002
    ..A recent publication in Cell describes how adenovirus can disrupt cell junctions, thereby effecting its release from basal surfaces of an infected epithelium to the apical or external environment...
  18. ncbi Search for polymorphisms in the genes for herpesvirus entry mediator, nectin-1, and nectin-2 in immune seronegative individuals
    Frank Struyf
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Infect Dis 185:36-44. 2002
    ....
  19. pmc Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry
    Miri Yoon
    Department of Microbiology Immunology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 76:7203-8. 2002
    ....
  20. pmc Amino acid substitutions in the V domain of nectin-1 (HveC) that impair entry activity for herpes simplex virus types 1 and 2 but not for Pseudorabies virus or bovine herpesvirus 1
    Wanda M Martinez
    Department of Microbiology Immunology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 76:7255-62. 2002
    ..Thus, amino acids 77 and 85 of nectin-1 form part of the interface with HSV gD or influence the conformation of that interface. Moreover, the binding sites for HSV and PRV or BHV-1 gDs on nectin-1 may overlap but are not identical...
  21. pmc Infection of neurons and encephalitis after intracranial inoculation of herpes simplex virus requires the entry receptor nectin-1
    Sarah J Kopp
    Department of Microbiology Immunology, Feinberg School of Medicine of Northwestern University, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 106:17916-20. 2009
    ....
  22. pmc Insertion mutations in herpes simplex virus 1 glycoprotein H reduce cell surface expression, slow the rate of cell fusion, or abrogate functions in cell fusion and viral entry
    Julia O Jackson
    Department of Microbiology and Immunology, The Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Ward 6 231, Chicago, IL 60611, USA
    J Virol 84:2038-46. 2010
    ....
  23. pmc PILRalpha is a herpes simplex virus-1 entry coreceptor that associates with glycoprotein B
    Takeshi Satoh
    Department of Immunochemistry, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
    Cell 132:935-44. 2008
    ..These findings uncover a crucial aspect of the mechanism underlying HSV-1 infection...
  24. ncbi Inhibition of TCR signaling by herpes simplex virus
    Derek D Sloan
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    J Immunol 176:1825-33. 2006
    ..Our findings elucidate a potentially novel method of viral immune evasion that could be exploited to better manage HSV infection, aid in vaccine design, or allow targeted manipulation of T cell function...
  25. pmc Evolutionarily divergent herpesviruses modulate T cell activation by targeting the herpesvirus entry mediator cosignaling pathway
    Timothy C Cheung
    Division of Molecular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Proc Natl Acad Sci U S A 102:13218-23. 2005
    ..The demonstration that distinct herpesviruses target the HVEM-BTLA cosignaling pathway suggests the importance of this pathway in regulating T cell activation during host defenses...
  26. pmc Biography of Patricia G. Spear
    Liza Q Bundesen
    Proc Natl Acad Sci U S A 101:12411-3. 2004
  27. pmc The principal neuronal gD-type 3-O-sulfotransferases and their products in central and peripheral nervous system tissues
    Roger Lawrence
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Matrix Biol 26:442-55. 2007
    ..Thus, 3-OST-2 and 3-OST-4 are the major neural gD-type 3-OSTs, and so are prime candidates for participating in HS-dependent neurobiologic events...
  28. pmc Cross-regulation between herpesviruses and the TNF superfamily members
    John R Sedy
    Division of Molecular Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA
    Nat Rev Immunol 8:861-73. 2008
    ..Despite these strong immune responses, herpesviruses persist in a latent form, which suggests a dynamic relationship between the host immune system and the virus that results in a balance between host survival and viral control...
  29. ncbi Biochemistry. Viral glycoproteins and an evolutionary conundrum
    Alasdair C Steven
    Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, 50 South Drive, Bethesda, MD 20892, USA
    Science 313:177-8. 2006
  30. pmc Regulation of Notch signaling by Drosophila heparan sulfate 3-O sulfotransferase
    Keisuke Kamimura
    Dept of Genetics, Cell Biology, and Development, University of Minnesota, 6 160 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA
    J Cell Biol 166:1069-79. 2004
    ..These findings suggest that Hs3st-B is involved in Notch signaling by affecting stability or intracellular trafficking of Notch protein...

Research Grants17

  1. HERPESVIRUS GENE EXPRESSION IN TRANSFORMED CELLS
    PATRICIA SPEAR; Fiscal Year: 2007
    ....
  2. ENTRY OF HERPES SIMPLEX VIRUS INTO CELLS
    PATRICIA SPEAR; Fiscal Year: 2007
    ..abstract_text> ..
  3. HERPESVIRUS GENE EXPRESSION IN TRANSFORMED CELLS
    PATRICIA SPEAR; Fiscal Year: 2005
    ....
  4. INTL CONG OF VIROLOGY, SAN FRANCISCO, ASV TRAVEL REQUEST
    PATRICIA SPEAR; Fiscal Year: 2005
    ..Dr. Patricia Spear, ASV President, as P.I...
  5. Herpes simplex virus receptors and signal transduction
    PATRICIA SPEAR; Fiscal Year: 2003
    ..The studies described in this proposal are distinct from those supported by the parent grant but address questions that arise from findings made in the parent project. ..
  6. HERPESVIRUS GENE EXPRESSION IN TRANSFORMED CELLS
    PATRICIA SPEAR; Fiscal Year: 2000
    ....