Research TopicsGenomes and GenesSpecies | PATRICIA SPEARSummaryAffiliation: Northwestern University Country: USA Publications
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Publications
Different receptors binding to distinct interfaces on herpes simplex virus gD can trigger events leading to cell fusion and viral entryPatricia G Spear
Department of Microbiology Immunology, MC S213, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611, USA
Virology 344:17-24. 2006..Finally, information and ideas are presented about a membrane-proximal region of gD that is required for membrane fusion, but not for receptor binding, and that may have a role in activating the fusogenic activity of gB, gH and gL...- Herpes simplex virus: receptors and ligands for cell entryPatricia G Spear
Feinberg School of Medicine of Northwestern University, Chicago, IL 60611, USA
Cell Microbiol 6:401-10. 2004.... 
Herpesvirus entry: an updatePatricia G Spear
Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
J Virol 77:10179-85. 2003- Mutations in the N termini of herpes simplex virus type 1 and 2 gDs alter functional interactions with the entry/fusion receptors HVEM, nectin-2, and 3-O-sulfated heparan sulfate but not with nectin-1Miri Yoon
Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
J Virol 77:9221-31. 2003..The sequence of the N terminus determines whether nectin-2 or 3-O-sulfated heparan sulfate, as well as HVEM, can serve as entry/fusion receptors... - Differential effects on cell fusion activity of mutations in herpes simplex virus 1 glycoprotein B (gB) dependent on whether a gD receptor or a gB receptor is overexpressedQing Fan
Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
J Virol 83:7384-90. 2009.... - Alanine substitution of conserved residues in the cytoplasmic tail of herpes simplex virus gB can enhance or abolish cell fusion activity and viral entryNancy Ruel
Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611, USA
Virology 346:229-37. 2006..Other mutations, particularly the deletion, significantly enhanced cell fusion activity. These mutations, as well as others described previously, identify regions of the gB cytoplasmic domain that modulate cell fusion activity... - Use of herpes simplex virus and pseudorabies virus chimeric glycoprotein D molecules to identify regions critical for membrane fusionAnna Zago
Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Proc Natl Acad Sci U S A 101:17498-503. 2004.... - Effects of linker-insertion mutations in herpes simplex virus 1 gD on glycoprotein-induced fusion with cells expressing HVEM or nectin-1Cheryl R Jogger
Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Virology 318:318-26. 2004..These and other results indicate that the mutations disruptive of interactions with both receptors probably affect conformations of contact sites that are different for each receptor... - Differences in the N termini of herpes simplex virus type 1 and 2 gDs that influence functional interactions with the human entry receptor Nectin-2 and an entry receptor expressed in Chinese hamster ovary cellsAnna Zago
Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
J Virol 77:9695-9. 2003.... - Mutations in the N-terminal domains of nectin-1 and nectin-2 reveal differences in requirements for entry of various alphaherpesviruses and for nectin-nectin interactionsFrank Struyf
Department of Microbiology Immunology, The Feinberg School of Medicine, Northwestern University, 320 E Superior Street, Chicago, IL 60611, USA
J Virol 76:12940-50. 2002..We can conclude that structural requirements for HSV entry, PRV and BHV-1 entry, and homotypic and heterotypic trans interactions are all different despite the previously reported ability of HSV and HSV gD to inhibit trans interactions... - Mutations in herpes simplex virus glycoprotein D that prevent cell entry via nectins and alter cell tropismSharmila Manoj
Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Proc Natl Acad Sci U S A 101:12414-21. 2004.... - Random mutagenesis of the gene encoding a viral ligand for multiple cell entry receptors to obtain viral mutants altered for receptor usageMiri Yoon
Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Proc Natl Acad Sci U S A 101:17252-7. 2004..These and other results show that features of gD, including conformation of the N terminus, critical for functional interactions with HVEM/3-O-sulfated heparan sulfate, differ from those critical for interactions with nectin-1... - Deletion of the second immunoglobulin-like domain of nectin-1 alters its intracellular processing and localization and ability to mediate entry of herpes simplex virusFrank Struyf
Northwestern University, The Feinberg School of Medicine, Department of Microbiology and Immunology, Searle 6 447, 320 E Superior St, Chicago, IL 60611, USA
J Virol 79:3841-5. 2005..The failure of nectin-1-Delta2 to mediate HSV entry probably resulted from its failure to be transported to a membrane targeted by HSV for viral entry... - Random linker-insertion mutagenesis to identify functional domains of herpes simplex virus type 1 glycoprotein BErick Lin
Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, MC S213, 320 East Superior Street, Chicago, IL 60611, USA
Proc Natl Acad Sci U S A 104:13140-5. 2007..Ten were between residues exposed to the surface of the trimer, identifying regions that may be critical for interactions with other viral proteins or cellular components or for transitions from the prefusion to postfusion state... - Alternative entry receptors for herpes simplex virus and their roles in diseaseJoann M Taylor
Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Cell Host Microbe 2:19-28. 2007..This study illustrates the value of mutant mice for understanding receptor contributions to disease caused by a human virus... - Insertional mutations in herpes simplex virus type 1 gL identify functional domains for association with gH and for membrane fusionQing Fan
Department of Microbiology Immunology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
J Virol 83:11607-15. 2009.... - Viral interactions with receptors in cell junctions and effects on junctional stabilityPatricia G Spear
Department of Microbiology-Immunology, The Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Dev Cell 3:462-4. 2002..A recent publication in Cell describes how adenovirus can disrupt cell junctions, thereby effecting its release from basal surfaces of an infected epithelium to the apical or external environment... - Search for polymorphisms in the genes for herpesvirus entry mediator, nectin-1, and nectin-2 in immune seronegative individualsFrank Struyf
Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
J Infect Dis 185:36-44. 2002.... - Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entryMiri Yoon
Department of Microbiology Immunology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
J Virol 76:7203-8. 2002.... - Amino acid substitutions in the V domain of nectin-1 (HveC) that impair entry activity for herpes simplex virus types 1 and 2 but not for Pseudorabies virus or bovine herpesvirus 1Wanda M Martinez
Department of Microbiology-Immunology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
J Virol 76:7255-62. 2002..Thus, amino acids 77 and 85 of nectin-1 form part of the interface with HSV gD or influence the conformation of that interface. Moreover, the binding sites for HSV and PRV or BHV-1 gDs on nectin-1 may overlap but are not identical... - Infection of neurons and encephalitis after intracranial inoculation of herpes simplex virus requires the entry receptor nectin-1Sarah J Kopp
Department of Microbiology Immunology, Feinberg School of Medicine of Northwestern University, Chicago, IL 60611, USA
Proc Natl Acad Sci U S A 106:17916-20. 2009.... - Insertion mutations in herpes simplex virus 1 glycoprotein H reduce cell surface expression, slow the rate of cell fusion, or abrogate functions in cell fusion and viral entryJulia O Jackson
Department of Microbiology and Immunology, The Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Ward 6 231, Chicago, IL 60611, USA
J Virol 84:2038-46. 2010.... - PILRalpha is a herpes simplex virus-1 entry coreceptor that associates with glycoprotein BTakeshi Satoh
Department of Immunochemistry, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565 0871, Japan
Cell 132:935-44. 2008..These findings uncover a crucial aspect of the mechanism underlying HSV-1 infection... - Inhibition of TCR signaling by herpes simplex virusDerek D Sloan
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
J Immunol 176:1825-33. 2006..Our findings elucidate a potentially novel method of viral immune evasion that could be exploited to better manage HSV infection, aid in vaccine design, or allow targeted manipulation of T cell function... - Evolutionarily divergent herpesviruses modulate T cell activation by targeting the herpesvirus entry mediator cosignaling pathwayTimothy C Cheung
Division of Molecular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
Proc Natl Acad Sci U S A 102:13218-23. 2005..The demonstration that distinct herpesviruses target the HVEM-BTLA cosignaling pathway suggests the importance of this pathway in regulating T cell activation during host defenses... - Biography of Patricia G. SpearLiza Q Bundesen
Proc Natl Acad Sci U S A 101:12411-3. 2004 - The principal neuronal gD-type 3-O-sulfotransferases and their products in central and peripheral nervous system tissuesRoger Lawrence
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Matrix Biol 26:442-55. 2007..Thus, 3-OST-2 and 3-OST-4 are the major neural gD-type 3-OSTs, and so are prime candidates for participating in HS-dependent neurobiologic events... - Cross-regulation between herpesviruses and the TNF superfamily membersJohn R Sedy
Division of Molecular Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA
Nat Rev Immunol 8:861-73. 2008..Despite these strong immune responses, herpesviruses persist in a latent form, which suggests a dynamic relationship between the host immune system and the virus that results in a balance between host survival and viral control... - Biochemistry. Viral glycoproteins and an evolutionary conundrumAlasdair C Steven
Laboratory of Structural Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, 50 South Drive, Bethesda, MD 20892, USA
Science 313:177-8. 2006 - Regulation of Notch signaling by Drosophila heparan sulfate 3-O sulfotransferaseKeisuke Kamimura
Dept of Genetics, Cell Biology, and Development, University of Minnesota, 6 160 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA
J Cell Biol 166:1069-79. 2004..These findings suggest that Hs3st-B is involved in Notch signaling by affecting stability or intracellular trafficking of Notch protein...
Research Grants
- HERPESVIRUS GENE EXPRESSION IN TRANSFORMED CELLSPATRICIA SPEAR; Fiscal Year: 2007....
- ENTRY OF HERPES SIMPLEX VIRUS INTO CELLSPATRICIA SPEAR; Fiscal Year: 2007..abstract_text> ..
- HERPESVIRUS GENE EXPRESSION IN TRANSFORMED CELLSPATRICIA SPEAR; Fiscal Year: 2005....
- INTL CONG OF VIROLOGY, SAN FRANCISCO, ASV TRAVEL REQUESTPATRICIA SPEAR; Fiscal Year: 2005..Dr. Patricia Spear, ASV President, as P.I...
- Herpes simplex virus receptors and signal transductionPATRICIA SPEAR; Fiscal Year: 2003..The studies described in this proposal are distinct from those supported by the parent grant but address questions that arise from findings made in the parent project. ..
- HERPESVIRUS GENE EXPRESSION IN TRANSFORMED CELLSPATRICIA SPEAR; Fiscal Year: 2000....