ERIK SONTHEIMER

Summary

Affiliation: Northwestern University
Country: USA

Publications

  1. doi request reprint Small RNAs of opposite sign… but same absolute value
    Erik J Sontheimer
    Department of Molecular Biosciences, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
    Cell 151:1157-8. 2012
  2. ncbi request reprint Silence from within: endogenous siRNAs and miRNAs
    Erik J Sontheimer
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
    Cell 122:9-12. 2005
  3. ncbi request reprint Assembly and function of RNA silencing complexes
    Erik J Sontheimer
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208 3500, USA
    Nat Rev Mol Cell Biol 6:127-38. 2005
  4. ncbi request reprint Molecular biology. Argonaute journeys into the heart of RISC
    Erik J Sontheimer
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Science 305:1409-10. 2004
  5. ncbi request reprint Short interfering RNA strand selection is independent of dsRNA processing polarity during RNAi in Drosophila
    Jonathan B Preall
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
    Curr Biol 16:530-5. 2006
  6. ncbi request reprint The Making of an siRNA
    John W Pham
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Mol Cell 15:163-4. 2004
  7. pmc Origins and Mechanisms of miRNAs and siRNAs
    Richard W Carthew
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
    Cell 136:642-55. 2009
  8. pmc Self versus non-self discrimination during CRISPR RNA-directed immunity
    Luciano A Marraffini
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
    Nature 463:568-71. 2010
  9. ncbi request reprint Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways
    Young Sik Lee
    Department of Biochemistry, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Cell 117:69-81. 2004
  10. ncbi request reprint RNAi: RISC gets loaded
    Jonathan B Preall
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Cell 123:543-5. 2005

Research Grants

  1. Mechanisms of CRISPR Interference
    Erik J Sontheimer; Fiscal Year: 2010
  2. RNA Silencing Complex Assembly and Function
    ERIK SONTHEIMER; Fiscal Year: 2007
  3. Improvement of RNAi efficacy by blocking RNAi inhibitors
    ERIK SONTHEIMER; Fiscal Year: 2007

Collaborators

Detail Information

Publications23

  1. doi request reprint Small RNAs of opposite sign… but same absolute value
    Erik J Sontheimer
    Department of Molecular Biosciences, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
    Cell 151:1157-8. 2012
    ..Fang et al. now reveal a set of Piwi-interacting RNAs (piRNAs) in Oxytricha trifallax that likewise enable genomic self versus nonself discrimination, this time by specifying self sequences for genome retention...
  2. ncbi request reprint Silence from within: endogenous siRNAs and miRNAs
    Erik J Sontheimer
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
    Cell 122:9-12. 2005
    ..Bioinformatic analyses indicate that target genes in vertebrate species may number in the thousands...
  3. ncbi request reprint Assembly and function of RNA silencing complexes
    Erik J Sontheimer
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208 3500, USA
    Nat Rev Mol Cell Biol 6:127-38. 2005
    ..These advances have taken our mechanistic understanding of RNA interference to a new level and promise to improve our ability to exploit this biological process for use in experimental biology and medicine...
  4. ncbi request reprint Molecular biology. Argonaute journeys into the heart of RISC
    Erik J Sontheimer
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Science 305:1409-10. 2004
  5. ncbi request reprint Short interfering RNA strand selection is independent of dsRNA processing polarity during RNAi in Drosophila
    Jonathan B Preall
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
    Curr Biol 16:530-5. 2006
    ..Combined dsRNA-processing/mRNA-cleavage assays indicate that siRNA strand selection is independent of dsRNA processing polarity during Drosophila RISC assembly in vitro...
  6. ncbi request reprint The Making of an siRNA
    John W Pham
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Mol Cell 15:163-4. 2004
    ..A recent report in Cell reveals how Dicer's two RNase III domains collaborate during dsRNA processing and sets the stage for a deeper understanding of Dicer's roles in later phases of silencing complex assembly...
  7. pmc Origins and Mechanisms of miRNAs and siRNAs
    Richard W Carthew
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
    Cell 136:642-55. 2009
    ..Our understanding of siRNA- and miRNA-based regulation has direct implications for fundamental biology as well as disease etiology and treatment...
  8. pmc Self versus non-self discrimination during CRISPR RNA-directed immunity
    Luciano A Marraffini
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
    Nature 463:568-71. 2010
    ..Differential complementarity outside of the spacer sequence is a built-in feature of all CRISPR systems, indicating that this mechanism is a broadly applicable solution to the self/non-self dilemma that confronts all immune pathways...
  9. ncbi request reprint Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways
    Young Sik Lee
    Department of Biochemistry, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Cell 117:69-81. 2004
    ..However, Dicer-1 but not Dicer-2 is essential for miRISC-directed translation repression. Thus, siRISCs and miRISCs are different with respect to Dicers in Drosophila...
  10. ncbi request reprint RNAi: RISC gets loaded
    Jonathan B Preall
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Cell 123:543-5. 2005
    ..2005) reveal the fate of the rejected passenger siRNA strand. Additionally, Gregory et al. (2005) define a heterotrimeric complex from humans that appears to execute dsRNA loading, strand selection, and target mRNA cleavage activities...
  11. pmc Silencing by small RNAs is linked to endosomal trafficking
    Young Sik Lee
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60201, USA
    Nat Cell Biol 11:1150-6. 2009
    ..We suggest that the recycling of RISCs is promoted by MVBs, resulting in RISCs more effectively engaging with small RNA effectors and possibly target RNAs. It may provide a means to enhance the dynamics of RNA silencing in the cytoplasm...
  12. pmc CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNA
    Luciano A Marraffini
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Science 322:1843-5. 2008
    ..We conclude that CRISPR loci counteract multiple routes of HGT and can limit the spread of antibiotic resistance in pathogenic bacteria...
  13. ncbi request reprint Molecular requirements for RNA-induced silencing complex assembly in the Drosophila RNA interference pathway
    John W Pham
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
    J Biol Chem 280:39278-83. 2005
    ....
  14. ncbi request reprint A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila
    John W Pham
    Department of Biochemistry, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Cell 117:83-94. 2004
    ..Dcr-2 does not simply transfer siRNAs to a distinct effector complex, but rather assembles into RISC along with the siRNAs, indicating that its role extends beyond the initiation phase of RNAi...
  15. ncbi request reprint R2D2 leads the silencing trigger to mRNA's death star
    Janice L Pellino
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Cell 115:132-3. 2003
    ..Now, Liu et al. identify R2D2, a Dicer-associated protein that is important for siRNA incorporation into RISC, thus linking the initiation and execution phases of RNAi...
  16. pmc "siRNAs and miRNAs": a meeting report on RNA silencing
    Zhengying He
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
    RNA 10:1165-73. 2004
  17. ncbi request reprint Separation of Drosophila RNA silencing complexes by native gel electrophoresis
    John W Pham
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL, USA
    Methods Mol Biol 309:11-6. 2005
  18. pmc ATP modulates siRNA interactions with an endogenous human Dicer complex
    Janice L Pellino
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208 3500, USA
    RNA 11:1719-24. 2005
    ..Complex D could impinge upon the RISC assembly pathway in humans, similar to an analogous complex in Drosophila...
  19. pmc Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p
    Priya Bellare
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    RNA 12:292-302. 2006
    ..Our results define a new UBD and suggest functional links between ubiquitin and the pre-mRNA splicing machinery...
  20. pmc An inside job for siRNAs
    Daniel E Golden
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Mol Cell 31:309-12. 2008
    ..Recent work in flies and mice reveals several classes of endogenous siRNAs (endo-siRNAs) that contribute to functions previously reserved for miRNAs and piRNAs, including gene regulation and transposon suppression...
  21. pmc Invasive DNA, chopped and in the CRISPR
    Luciano A Marraffini
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Structure 17:786-8. 2009
    ..describe the structure and activity of Cas1, the only protein associated with all CRISPR loci. Cas1 is a metal-dependent deoxyribonuclease, consistent with a role in the adaptation phase of CRISPR immunity against invading nucleic acids...
  22. pmc Thermodynamic and structural characterization of 2'-nitrogen-modified RNA duplexes
    John W Pham
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
    Nucleic Acids Res 32:3446-55. 2004
    ..These results indicate that 2'-ureido modifications are superior to analogous 2'-amido ones for applications that require stable base pairing...
  23. ncbi request reprint A fork in the road for microRNAs
    Priya Bellare
    Nat Struct Mol Biol 14:684-6. 2007

Research Grants6

  1. Mechanisms of CRISPR Interference
    Erik J Sontheimer; Fiscal Year: 2010
    ..The proposed studies will clarify the molecular basis for CRISPR function and will therefore contribute to our ability to exploit this natural pathway to prevent or treat infectious disease. ..
  2. RNA Silencing Complex Assembly and Function
    ERIK SONTHEIMER; Fiscal Year: 2007
    ..Accordingly, a deeper understanding of RNA silencing pathways will accelerate many aspects of biomedical research and disease treatment. ..
  3. Improvement of RNAi efficacy by blocking RNAi inhibitors
    ERIK SONTHEIMER; Fiscal Year: 2007
    ..Development of compounds that boost RNAi performance in general will have significant impact on public health. ..