LONNIE D contact SHEA

Summary

Affiliation: Northwestern University
Country: USA

Publications

  1. pmc Back to the science of stem cell research - CHI's 2nd Annual Meeting
    Lonnie D Shea
    Northwestern University, Evanston, IL 60208, USA
    IDrugs 9:699-701. 2006
  2. pmc The role of the extracellular matrix in ovarian follicle development
    Teresa K Woodruff
    Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA
    Reprod Sci 14:6-10. 2007
  3. pmc Gene delivery by surface immobilization of plasmid to tissue-engineering scaffolds
    D M Salvay
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208 3120, USA
    Gene Ther 17:1134-41. 2010
  4. pmc Dynamic transcription factor networks in epithelial-mesenchymal transition in breast cancer models
    Anaar Siletz
    Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, Illinois, United States of America
    PLoS ONE 8:e57180. 2013
  5. pmc Dynamic, large-scale profiling of transcription factor activity from live cells in 3D culture
    Michael S Weiss
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, United States of America
    PLoS ONE 5:e14026. 2010
  6. pmc Hydrogels for lentiviral gene delivery
    Stephanie K Seidlits
    Northwestern University, Department of Chemical and Biological Engineering, 2145 Sheridan Rd, Tech Building E 136, Evanston, IL 60208, USA
    Expert Opin Drug Deliv 10:499-509. 2013
  7. ncbi request reprint Timing is everything the role of kinetics in G protein activation
    L D Shea
    Department of Chemical Engineering, Northwestern University, Evanston, IL 60208, USA
    Life Sci 68:647-58. 2000
  8. pmc Local gene delivery from ECM-coated poly(lactide-co-glycolide) multiple channel bridges after spinal cord injury
    Laura De Laporte
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, E156 Evanston, IL 60208 3120, USA
    Biomaterials 30:2361-8. 2009
  9. ncbi request reprint Modular design of non-viral vectors with bioactive components
    Lonnie D Shea
    Departments of Chemical and Biological Engineering and Biomedical Engineering, Northwestern University, 2145 Sheridan Rd E156, Evanston, IL 60208 3120, USA
    Trends Biotechnol 22:429-31. 2004
  10. pmc Patterned transgene expression in multiple-channel bridges after spinal cord injury
    Laura De Laporte
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road E156, Evanston, IL 60208 3120, USA
    Acta Biomater 6:2889-97. 2010

Research Grants

  1. Substrate mediated DNA delivery
    Lonnie Shea; Fiscal Year: 2003
  2. Controlled Release Scaffolds for Nerve Regeneration
    Lonnie D Shea; Fiscal Year: 2010
  3. Controlled Release Scaffolds for Nerve Regeneration
    Lonnie Shea; Fiscal Year: 2009
  4. Controlled Release Scaffolds for Nerve Regeneration
    Lonnie Shea; Fiscal Year: 2009
  5. BIOTECHNOLOGY PREDOCTORAL TRAINING PROGRAM
    Lonnie Shea; Fiscal Year: 2007
  6. Parallel gene delivery from spinal cord bridges
    Lonnie Shea; Fiscal Year: 2007
  7. Substrate mediated DNA delivery
    Lonnie Shea; Fiscal Year: 2007
  8. Controlled Release Scaffolds for Nerve Regeneration
    Lonnie Shea; Fiscal Year: 2007
  9. Transfected cell arrays for cancer research
    Lonnie Shea; Fiscal Year: 2007
  10. Substrate mediated DNA delivery
    Lonnie Shea; Fiscal Year: 2006

Collaborators

Detail Information

Publications71

  1. pmc Back to the science of stem cell research - CHI's 2nd Annual Meeting
    Lonnie D Shea
    Northwestern University, Evanston, IL 60208, USA
    IDrugs 9:699-701. 2006
  2. pmc The role of the extracellular matrix in ovarian follicle development
    Teresa K Woodruff
    Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA
    Reprod Sci 14:6-10. 2007
    ..The process of in-follicle maturation provides a new tool for understanding ovarian follicle development under the influence of these factors...
  3. pmc Gene delivery by surface immobilization of plasmid to tissue-engineering scaffolds
    D M Salvay
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208 3120, USA
    Gene Ther 17:1134-41. 2010
    ....
  4. pmc Dynamic transcription factor networks in epithelial-mesenchymal transition in breast cancer models
    Anaar Siletz
    Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, Illinois, United States of America
    PLoS ONE 8:e57180. 2013
    ..These analyses show EMT from a unique and targetable vantage and may ultimately contribute to diagnosis and therapy...
  5. pmc Dynamic, large-scale profiling of transcription factor activity from live cells in 3D culture
    Michael S Weiss
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, United States of America
    PLoS ONE 5:e14026. 2010
    ..Taken together, our objective was to develop cellular arrays for dynamic, large-scale quantification of TF activity as cells organized into spherical structures within 3D culture...
  6. pmc Hydrogels for lentiviral gene delivery
    Stephanie K Seidlits
    Northwestern University, Department of Chemical and Biological Engineering, 2145 Sheridan Rd, Tech Building E 136, Evanston, IL 60208, USA
    Expert Opin Drug Deliv 10:499-509. 2013
    ..The high water content and mild gelation conditions of hydrogels support their use for gene delivery by preserving activity of lentiviral vectors and acting to shield vectors from any host immune response...
  7. ncbi request reprint Timing is everything the role of kinetics in G protein activation
    L D Shea
    Department of Chemical Engineering, Northwestern University, Evanston, IL 60208, USA
    Life Sci 68:647-58. 2000
    ..Incorporation of the reaction kinetics is critical for a complete understanding of signal transduction and will ultimately impact the fields of drug discovery and drug design...
  8. pmc Local gene delivery from ECM-coated poly(lactide-co-glycolide) multiple channel bridges after spinal cord injury
    Laura De Laporte
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, E156 Evanston, IL 60208 3120, USA
    Biomaterials 30:2361-8. 2009
    ..Additionally, expression with lipoplexes persisted for at least three weeks. Surface-mediated delivery can be applied to scaffolds with complex geometries to promote transgene expression in vivo...
  9. ncbi request reprint Modular design of non-viral vectors with bioactive components
    Lonnie D Shea
    Departments of Chemical and Biological Engineering and Biomedical Engineering, Northwestern University, 2145 Sheridan Rd E156, Evanston, IL 60208 3120, USA
    Trends Biotechnol 22:429-31. 2004
    ..Modular vectors containing bioactive components that target various cellular processes can overcome the barriers limiting gene transfer...
  10. pmc Patterned transgene expression in multiple-channel bridges after spinal cord injury
    Laura De Laporte
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road E156, Evanston, IL 60208 3120, USA
    Acta Biomater 6:2889-97. 2010
    ..This surface immobilization strategy enables patterned gene delivery in vitro and in vivo on length scales of hundreds of microns and may find utility in strategies aimed at regenerating tissues with complex architectures...
  11. pmc Plasmid delivery in vivo from porous tissue-engineering scaffolds: transgene expression and cellular transfection
    Jae Hyung Jang
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road E156, Evanston, IL 60208 3120, USA
    Mol Ther 12:475-83. 2005
    ..Correlating scaffold design with gene transfer efficiency and tissue formation will facilitate application of plasmid-releasing scaffolds to multiple tissues...
  12. pmc Balancing cell migration with matrix degradation enhances gene delivery to cells cultured three-dimensionally within hydrogels
    Jaclyn A Shepard
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Tech E136, Evanston, IL 60208, USA
    J Control Release 146:128-35. 2010
    ..Thus, matrix degradation and cell migration are fundamental design parameters for maximizing gene delivery within hydrogels...
  13. pmc Surface adsorption of DNA to tissue engineering scaffolds for efficient gene delivery
    Jae Hyung Jang
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208 3120, USA
    J Biomed Mater Res A 77:50-8. 2006
    ..Tissue engineering scaffolds that are prefabricated into various shapes from a range of materials could potentially employ this strategy for numerous applications...
  14. pmc Gene expression and internalization following vector adsorption to immobilized proteins: dependence on protein identity and density
    Zain Bengali
    Department of Interdepartmental Biological Sciences, Northwestern University, 2145 Sheridan Rd E156, Evanston, IL 60208 3120, USA
    J Gene Med 9:668-78. 2007
    ..This report investigates the mechanism and specificity by which the protein coating enhances gene transfer, and determines if the protein coating targets the vector for internalization by a specific pathway...
  15. pmc Non-viral vector delivery from PEG-hyaluronic acid hydrogels
    Julie A Wieland
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208 3120, United States
    J Control Release 120:233-41. 2007
    ..These studies demonstrate the dependence of non-viral vector release on the physical properties of the hydrogel and the vector, suggesting vector and hydrogel designs for maximizing localized delivery of non-viral vectors...
  16. pmc Plasmid releasing multiple channel bridges for transgene expression after spinal cord injury
    Laura De Laporte
    1Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, USA
    Mol Ther 17:318-26. 2009
    ..This synergy between gene delivery and the scaffold architecture may enable the engineering of tissues with complex architectures...
  17. pmc Spatially patterned gene delivery for localized neuron survival and neurite extension
    Tiffany Houchin-Ray
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, USA
    Mol Ther 15:705-12. 2007
    ..This approach demonstrates the basic technology to create patterns of gene expression that can direct tissue formation and could be employed in regenerative strategies to recreate the complex cellular architectures observed in tissues...
  18. pmc Secondary follicle growth and oocyte maturation by culture in alginate hydrogel following cryopreservation of the ovary or individual follicles
    Min Xu
    Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA
    Biotechnol Bioeng 103:378-86. 2009
    ....
  19. pmc Distribution of extracellular matrix proteins type I collagen, type IV collagen, fibronectin, and laminin in mouse folliculogenesis
    Courtney B Berkholtz
    Interdepartmental Biological Sciences Program, Northwestern University, Evanston, IL, 60208, USA
    Histochem Cell Biol 126:583-92. 2006
    ..This understanding of ECM composition and distribution can be used in the basic studies of ECM function during follicle development, and could aid in the development of in vitro systems for follicle growth...
  20. pmc Self-assembling peptide-lipoplexes for substrate-mediated gene delivery
    Jennifer C Rea
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Tech E156, Evanston, IL 60208 3120, USA
    Acta Biomater 5:903-12. 2009
    ....
  21. ncbi request reprint Drug-releasing scaffolds fabricated from drug-loaded microspheres
    Moriah Nof
    Department of Chemical Engineering, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208 3120, USA
    J Biomed Mater Res 59:349-56. 2002
    ..This approach of assembling drug-loaded microspheres into porous and nonporous structures may find great utility in the fabrication of synthetic matrices that direct tissue formation...
  22. ncbi request reprint Delivery systems for small molecule drugs, proteins, and DNA: the neuroscience/biomaterial interface
    Kevin J Whittlesey
    Interdepartmental Biological Sciences Program, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Exp Neurol 190:1-16. 2004
    ..The biomaterial field offers unique experimental tools with downstream clinical application for the study and treatment of neurologic disease...
  23. pmc A new hypothesis regarding ovarian follicle development: ovarian rigidity as a regulator of selection and health
    Teresa K Woodruff
    Center for Reproductive Research, Northwestern University, Evanston, IL 60208, USA
    J Assist Reprod Genet 28:3-6. 2011
    ..This novel perspective on ovarian function may provide new avenues to study follicle dynamics and identify therapeutic targets for ovarian dysfunction...
  24. pmc Engineering the follicle microenvironment
    Erin R West
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
    Semin Reprod Med 25:287-99. 2007
    ....
  25. pmc Gene delivery through cell culture substrate adsorbed DNA complexes
    Zain Bengali
    Department of Interdepartmental Biological Sciences, Northwestern University, 2145 Sheridan Rd E156, Evanston, Illinois 60208 3120, USA
    Biotechnol Bioeng 90:290-302. 2005
    ..Additionally, adapting this system to biomaterials may facilitate application to fields such as tissue engineering...
  26. pmc Matrices and scaffolds for DNA delivery in tissue engineering
    Laura De Laporte
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208 3120, USA
    Adv Drug Deliv Rev 59:292-307. 2007
    ..Strategies to achieve controlled, localized expression within the tissue engineering scaffold will have broad application to the regeneration of many tissues, with great promise for clinical therapies...
  27. ncbi request reprint Identification of a stage-specific permissive in vitro culture environment for follicle growth and oocyte development
    Min Xu
    Center for Reproductive Science, Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208, USA
    Biol Reprod 75:916-23. 2006
    ....
  28. pmc Efficacy of immobilized polyplexes and lipoplexes for substrate-mediated gene delivery
    Zain Bengali
    Department of Interdepartmental Biological Sciences, Northwestern University, Evanston, Illinois, USA
    Biotechnol Bioeng 102:1679-91. 2009
    ..These studies suggest that strategies to enhance surface delivery for polyplexes should target the vector design to enhance its potency, whereas enhancing lipoplex delivery should target the material design to increase internalization...
  29. ncbi request reprint Postnatal regulation of germ cells by activin: the establishment of the initial follicle pool
    Sarah K Bristol-Gould
    Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208, USA
    Dev Biol 298:132-48. 2006
    ..The proposed mechanism provides a means by which the ovary eliminates excess follicles containing oocytes of poor quality prior to puberty, thus maintaining fertility in the face of abnormal hormonal stimuli in the prepubertal period...
  30. pmc Phosphatidylserine immobilization of lentivirus for localized gene transfer
    Seungjin Shin
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208 3120, USA
    Biomaterials 31:4353-9. 2010
    ..This specific binding of lentiviral vectors to biomaterial scaffolds may provide a versatile tool for numerous applications in regenerative medicine or within model systems that investigate tissue development...
  31. ncbi request reprint Novel approach for the three-dimensional culture of granulosa cell-oocyte complexes
    Stephanie A Pangas
    Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208, USA
    Tissue Eng 9:1013-21. 2003
    ..Oocytes retrieved and matured were able to resume meiosis, a necessary step for proper development. Thus, this system represents a new in vitro methodology for growth of individual granulosa cell-oocyte complexes...
  32. pmc The in vitro regulation of ovarian follicle development using alginate-extracellular matrix gels
    Pamela K Kreeger
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Tech E136, Evanston, IL 60208, USA
    Biomaterials 27:714-23. 2006
    ....
  33. pmc Interpenetrating fibrin-alginate matrices for in vitro ovarian follicle development
    Ariella Shikanov
    Institute of Bionanotechnology in Medicine, Northwestern University, 303 E Superior St, Chicago, IL 60611, USA
    Biomaterials 30:5476-85. 2009
    ....
  34. pmc Intramuscular delivery of DNA releasing microspheres: microsphere properties and transgene expression
    Jae Hyung Jang
    Department of Chemical Engineering, University of California Berkeley, 201 Gilman Hall, Berkeley, CA 94720 1401, USA
    J Control Release 112:120-8. 2006
    ..This understanding of microsphere properties that determine transgene expression and the distribution of transfected cells may facilitate their application to fields such as tissue engineering or DNA vaccines...
  35. pmc Substrate-mediated delivery from self-assembled monolayers: effect of surface ionization, hydrophilicity, and patterning
    Angela K Pannier
    Department of Interdepartmental Biological Sciences, Northwestern University, 2145 Sheridan Road, E156, Evanston, IL 60208 3120, USA
    Acta Biomater 1:511-22. 2005
    ....
  36. ncbi request reprint Fate of the initial follicle pool: empirical and mathematical evidence supporting its sufficiency for adult fertility
    Sarah K Bristol-Gould
    Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208, USA
    Dev Biol 298:149-54. 2006
    ..Our results agree with established dogma that the initial endowment of ovarian follicles is not supplemented by an appreciable number of stem cells; rather, it is sufficient to ensure the fertility needs of the adult mouse...
  37. ncbi request reprint Sustained transgene expression via citric acid-based polyester elastomers
    Xue Qing Zhang
    Biomedical Engineering Department, Northwestern University, Evanston, IL 60208, USA
    Biomaterials 30:2632-41. 2009
    ..The results demonstrate that POC scaffolds are a suitable material for substrate-mediated gene delivery. POC scaffolds can potentially support long-term biological cues to mediate tissue formation through non-viral gene delivery...
  38. pmc Layered PLG scaffolds for in vivo plasmid delivery
    Christopher B Rives
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Tech E136, Evanston, IL 60208, USA
    Biomaterials 30:394-401. 2009
    ..Scaffold-based gene delivery systems capable of localized transgene expression provide a platform for inductive and cell transplantation approaches in regenerative medicine...
  39. pmc The mouse follicle microenvironment regulates antrum formation and steroid production: alterations in gene expression profiles
    Erin R West-Farrell
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, USA
    Biol Reprod 80:432-9. 2009
    ....
  40. pmc Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation
    Shiying Jin
    Center for Reproductive Research, Northwestern University, Evanston, Illinois, USA
    Fertil Steril 93:2627-32. 2010
    ..To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles within ovarian tissue slices...
  41. pmc Lentivirus immobilization to nanoparticles for enhanced and localized delivery from hydrogels
    Seungjin Shin
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208 3120, USA
    Mol Ther 18:700-6. 2010
    ..This strategy of nanoparticle immobilization to stabilize and retain vectors is broadly applicable to hydrogels, and may provide a versatile tool to combine gene therapy and biomaterials for applications in regenerative medicine...
  42. pmc The contribution of plasmid design and release to in vivo gene expression following delivery from cationic polymer modified scaffolds
    Misael O Aviles
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Evanston, IL 60208, USA
    Biomaterials 31:1140-7. 2010
    ....
  43. pmc Peptide-mediated lipofection is governed by lipoplex physical properties and the density of surface-displayed amines
    Jennifer C Rea
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd E156, Evanston, Illinois 60208 3120, USA
    J Pharm Sci 97:4794-806. 2008
    ..The addition of peptides is a simple method of lipofection enhancement, as direct chemical modification of lipids is not necessary for increased transfection...
  44. ncbi request reprint Surface-tethered DNA complexes for enhanced gene delivery
    Tatiana Segura
    Department of Chemical Engineering, Northwestern University, 2145 Sheridan Road E156, Evanston, Illinois 60208 3120, USA
    Bioconjug Chem 13:621-9. 2002
    ..Surface tethering of DNA represents a promising approach to enhancing gene transfer and spatially controlling gene delivery, which may have applications to a multitude of fields ranging from tissue engineering to functional genomics...
  45. ncbi request reprint Controllable delivery of non-viral DNA from porous scaffolds
    Jae Hyung Jang
    Department of Chemical Engineering, Northwestern University, 2145 Sheridan Rd, E156, Evanston, IL 60208 3120, USA
    J Control Release 86:157-68. 2003
    ..The ability to create porous polymer scaffolds capable of controlled release rates may provide a means to enhance and regulate gene transfer within a developing tissue, which will increase their utility in tissue engineering...
  46. ncbi request reprint Substrate-mediated DNA delivery: role of the cationic polymer structure and extent of modification
    Tatiana Segura
    Department of Chemical Engineering, Northwestern University, 2145 Sheridan Road E156, Evanston, IL 60208 3120, USA
    J Control Release 93:69-84. 2003
    ..Enzymatic degradation of cationic polymers is not necessary for transfection. Additionally, the duration of transgene expression was extended for surface-mediated delivery relative to bolus delivery...
  47. ncbi request reprint Controlled release systems for DNA delivery
    Angela K Pannier
    Department of Interdepartmental Biological Sciences, Northwestern University, 2145 Sheridan Road, E156, Evanston, IL 60208 3120, USA
    Mol Ther 10:19-26. 2004
    ..g., biotin-avidin). This review examines the delivery of nonviral and viral vectors from natural and synthetic polymers and presents opportunities for continuing developments to increase their applicability...
  48. pmc DNA delivery from hyaluronic acid-collagen hydrogels via a substrate-mediated approach
    Tatiana Segura
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd E156, Evanston, IL 60208 3120, USA
    Biomaterials 26:1575-84. 2005
    ..Biomaterial-based gene delivery can be applicable to numerous tissue engineering applications, or as a tool to examine tissue formation...
  49. pmc Design of modular non-viral gene therapy vectors
    Laura De Laporte
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road E156, Evanston, IL 60208 3120, USA
    Biomaterials 27:947-54. 2006
    ..Ultimately, efficient vectors will expand the traditional applications of gene therapy within the clinic and may enable numerous other opportunities within diagnostics, biotechnology, and basic science research...
  50. ncbi request reprint Gene delivery from polymer scaffolds for tissue engineering
    Jae Hyung Jang
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd E156 Evanston, IL 60208 3120, USA
    Expert Rev Med Devices 1:127-38. 2004
    ..The utility of this approach will increase with the development of design parameters that correlate release and transgene expression, and with continued research into the biology of tissue formation...
  51. pmc Nerve growth factor expression by PLG-mediated lipofection
    Kevin J Whittlesey
    Interdepartmental Biological Sciences Program, Northwestern University, Evanston, IL 60208, USA
    Biomaterials 27:2477-86. 2006
    ..Combining this physical support with the ability to locally express neurotrophic factors will potentiate regeneration in nerve injury and disease models...
  52. pmc Patterned PLG substrates for localized DNA delivery and directed neurite extension
    Tiffany Houchin-Ray
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd E156, Evanston, IL 60208 3120, USA
    Biomaterials 28:2603-11. 2007
    ..This approach demonstrates the ability to combine gene delivery with physical guidance, and can be tailored to target specific axonal populations with varying neurotrophic factor requirements...
  53. pmc Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes
    Angela K Pannier
    Department of Interdepartmental Biological Sciences, Northwestern University, 2145 Sheridan Road E156, Evanston, IL 60208 3120, USA
    Acta Biomater 4:26-39. 2008
    ..The ability to control the morphology of the immobilized complexes and influence transfection levels through surface chemistry could be translated to scaffolds for gene delivery in tissue engineering and diagnostic applications...
  54. pmc Extracellular matrix functions in follicle maturation
    Courtney B Berkholtz
    Interdepartmental Biological Sciences Program, Northwestern University, Evanston, Illinois 60208 3520, USA
    Semin Reprod Med 24:262-9. 2006
    ..This article describes the ECM functionality on ovarian cells throughout development, and highlights the potential of developing technologies to identify structure-function relationships in follicle maturation...
  55. pmc Bioluminescence imaging for assessment and normalization in transfected cell arrays
    Angela K Pannier
    Department of Interdepartmental Biological Sciences, Northwestern University, Evanston, Illinois
    Biotechnol Bioeng 98:486-97. 2007
    ..This system provides a tool for basic science research, with potential application for the development of patient specific therapies...
  56. ncbi request reprint Murine granulosa cell morphology and function are regulated by a synthetic Arg-Gly-Asp matrix
    Pamela K Kreeger
    Department of Chemical Engineering, Northwestern University, 2145 Sheridan Road, Tech E136, Evanston, IL 60208, USA
    Mol Cell Endocrinol 205:1-10. 2003
    ..These results indicate that the density and identity of adhesion peptides regulate granulosa cell function. This system provides a mechanism to examine the granulosa cell-ECM interactions that occur during follicle maturation...
  57. ncbi request reprint Bioengineering and the ovarian follicle
    Min Xu
    Northwestern University, USA
    Cancer Treat Res 138:75-82. 2007
  58. pmc Neurotrophin releasing single and multiple lumen nerve conduits
    Yang Yang
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd E156, Evanston, IL 60208 3120, USA
    J Control Release 104:433-46. 2005
    ..Polymer conduits with controllable lumen diameters and protein release may enhance nerve regeneration by guiding and stimulating neurite outgrowth...
  59. ncbi request reprint Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern
    Tatiana Segura
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, E156 Evanston, IL 60208 3120, USA
    Biomaterials 26:359-71. 2005
    ..Furthermore, the hydrogels could be functionalized with the biomolecule neutravidin by incorporation of biotin along the HA backbone. This biotinylation approach may allow attachment of bioactive molecules that are conjugated to avidin...
  60. pmc Physical properties of alginate hydrogels and their effects on in vitro follicle development
    Erin R West
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Tech E136, Evanston, IL 60208, USA
    Biomaterials 28:4439-48. 2007
    ..These studies reveal, for the first time, a direct link between the biomechanical environment and follicle function, and suggest a novel non-hormonal mechanism regulating follicle development...
  61. pmc A novel two-step strategy for in vitro culture of early-stage ovarian follicles in the mouse
    Shi Ying Jin
    Center for Reproductive Research, Northwestern University, Evanston, Illinois, USA
    Fertil Steril 93:2633-9. 2010
    ..To develop an in vitro strategy to support the growth of early-stage follicles and produce mature oocytes competent for fertilization...
  62. pmc Tissue-engineered follicles produce live, fertile offspring
    Min Xu
    Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208, USA
    Tissue Eng 12:2739-46. 2006
    ..This system creates new opportunities for discovery in follicle biology and establishes a core technology for human egg banks for preservation of fertility...
  63. pmc Lentivirus delivery by adsorption to tissue engineering scaffolds
    Seungjin Shin
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road E156, Evanston, Illinois 60208 3120, USA
    J Biomed Mater Res A 93:1252-9. 2010
    ..Delivery of lentiviral vectors from PLG scaffolds could provide an efficient and versatile gene delivery system for use with in vitro and in vivo models of tissue formation, and ultimately for therapeutic applications...
  64. pmc Inductive tissue engineering with protein and DNA-releasing scaffolds
    David M Salvay
    Department of Chemical and Biological Engineering, 2145 Sheridan Rd E156 Evanston, IL 60208 3120, USA
    Mol Biosyst 2:36-48. 2006
    ....
  65. pmc In vitro grown human ovarian follicles from cancer patients support oocyte growth
    Min Xu
    Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Hum Reprod 24:2531-40. 2009
    ..However, this method may risk re-introduction of the original cancer to the survivor of the disease. Thus, developing a method for in vitro growth of immature follicles may provide a method for fertility restoration in the future...
  66. pmc Multiple channel bridges for spinal cord injury: cellular characterization of host response
    Yang Yang
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208 3120, USA
    Tissue Eng Part A 15:3283-95. 2009
    ..Multiple channel bridges capable of supporting cellular infiltration, creating a permissive environment, and directing the growth of neural fibers have potential for promoting and directing spinal cord regeneration...
  67. pmc Motility-related actinin alpha-4 is associated with advanced and metastatic ovarian carcinoma
    Maria V Barbolina
    Department of Chemical and Biochemical Engineering, Northwestern University, Chicago, IL, USA
    Lab Invest 88:602-14. 2008
    ..Expression of ACTN4 in human ovarian tumors was found to be associated with advanced-stage disease and peritoneal metastases...
  68. pmc Regulation of mouse follicle development by follicle-stimulating hormone in a three-dimensional in vitro culture system is dependent on follicle stage and dose
    Pamela K Kreeger
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, USA
    Biol Reprod 73:942-50. 2005
    ..The culture system can be adapted to each stage of development, which will be especially critical for translation to human follicles that have a longer developmental period...
  69. ncbi request reprint Regulation and guidance of cell behavior for tissue regeneration via the siRNA mechanism
    Sangeeta K Cheema
    Department of Plastic Surgery, and Department of Experimental Therapeutics, The University of Texas M D Anderson Cancer Center, Houston, Texas 77230 1402, USA
    Wound Repair Regen 15:286-95. 2007
    ..siRNA represents a powerful tool to investigate and/or promote tissue formation, and numerous opportunities exist for identifying targets that promote regeneration of tissue and developing effective delivery systems...
  70. pmc The structures that underlie normal reproductive function
    Thomas F Lerch
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Mol Cell Endocrinol 267:1-5. 2007
    ..These three examples of structure informing function help explain reproductive health and may provide solutions to reproductive disease...
  71. doi request reprint Wilms tumor gene protein 1 is associated with ovarian cancer metastasis and modulates cell invasion
    Maria V Barbolina
    Department of Chemical and Biological Engineering, Northwestern University, Chicago, Illinois, USA
    Cancer 112:1632-41. 2008
    ..Metastasizing ovarian carcinoma cells encounter a collagen-rich microenvironment because the submesothelial matrix is comprised mainly of interstitial collagens Types I and III...

Research Grants19

  1. Substrate mediated DNA delivery
    Lonnie Shea; Fiscal Year: 2003
    ..This system will provide the tools to recreate the complex patterns of gene expression that are observed within a developing tissue. ..
  2. Controlled Release Scaffolds for Nerve Regeneration
    Lonnie D Shea; Fiscal Year: 2010
    ..Specific Aim 4: Investigate dual delivery of neurorophin-encoding plasmid and chondroitinase to enable axons crossing the bridge, the focus of Aim 3, to re-enter the host tissue. ..
  3. Controlled Release Scaffolds for Nerve Regeneration
    Lonnie Shea; Fiscal Year: 2009
    ..Specific Aim 4: Investigate dual delivery of neurorophin-encoding plasmid and chondroitinase to enable axons crossing the bridge, the focus of Aim 3, to re-enter the host tissue. ..
  4. Controlled Release Scaffolds for Nerve Regeneration
    Lonnie Shea; Fiscal Year: 2009
    ..Specific Aim 4: Investigate dual delivery of neurorophin-encoding plasmid and chondroitinase to enable axons crossing the bridge, the focus of Aim 3, to re-enter the host tissue. ..
  5. BIOTECHNOLOGY PREDOCTORAL TRAINING PROGRAM
    Lonnie Shea; Fiscal Year: 2007
    ..The number of preceptors participating in the Program will increase from 18 to 31. An increase from 5 to 8 funded positions will support this expanded preceptor base and allow the support of some Chemistry students. ..
  6. Parallel gene delivery from spinal cord bridges
    Lonnie Shea; Fiscal Year: 2007
    ..These results will broadly impact tissue engineering, as the regeneration of complex tissue architectures is a fundamental issue. ..
  7. Substrate mediated DNA delivery
    Lonnie Shea; Fiscal Year: 2007
    ..This system will provide the tools to recreate the complex patterns of gene expression that are observed within a developing tissue. ..
  8. Controlled Release Scaffolds for Nerve Regeneration
    Lonnie Shea; Fiscal Year: 2007
    ..Specific Aim 4: Investigate dual delivery of neurorophin-encoding plasmid and chondroitinase to enable axons crossing the bridge, the focus of Aim 3, to re-enter the host tissue. ..
  9. Transfected cell arrays for cancer research
    Lonnie Shea; Fiscal Year: 2007
    ..We will characterize TF activity for a) mammary epithelial cells immortalized by TERT (76NTERT) or by mutant p53 (76Ndel239) and b) primary tumor and malignant tumor cells, which were established from the same patient. ASSESSMENT: ..
  10. Substrate mediated DNA delivery
    Lonnie Shea; Fiscal Year: 2006
    ..This system will provide the tools to recreate the complex patterns of gene expression that are observed within a developing tissue. ..
  11. Substrate mediated DNA delivery
    Lonnie Shea; Fiscal Year: 2005
    ..This system will provide the tools to recreate the complex patterns of gene expression that are observed within a developing tissue. ..
  12. Substrate mediated DNA delivery
    Lonnie Shea; Fiscal Year: 2004
    ..This system will provide the tools to recreate the complex patterns of gene expression that are observed within a developing tissue. ..
  13. Protein-Releasing Microporous Scaffolds for Cell Replacement Therapy
    LONNIE D contact SHEA; Fiscal Year: 2010
    ..These scaffolds provide a support for cell growth and can deliver proteins which will be to enhance cell survival and function following islet transplantation. ..