Research Topics
Genomes and Genes | Luciano A MarraffiniSummaryAffiliation: Northwestern University Country: USA Publications
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Detail Information
Publications
CRISPR interference: RNA-directed adaptive immunity in bacteria and archaeaLuciano A Marraffini
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
Nat Rev Genet 11:181-90. 2010..Here, we review the mechanisms of CRISPR interference and its roles in microbial physiology and evolution. We also discuss potential applications of this novel interference pathway...
Self versus non-self discrimination during CRISPR RNA-directed immunityLuciano A Marraffini
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
Nature 463:568-71. 2010..Differential complementarity outside of the spacer sequence is a built-in feature of all CRISPR systems, indicating that this mechanism is a broadly applicable solution to the self/non-self dilemma that confronts all immune pathways...
CRISPR interference limits horizontal gene transfer in staphylococci by targeting DNALuciano A Marraffini
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
Science 322:1843-5. 2008..We conclude that CRISPR loci counteract multiple routes of HGT and can limit the spread of antibiotic resistance in pathogenic bacteria...
Invasive DNA, chopped and in the CRISPRLuciano A Marraffini
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
Structure 17:786-8. 2009..describe the structure and activity of Cas1, the only protein associated with all CRISPR loci. Cas1 is a metal-dependent deoxyribonuclease, consistent with a role in the adaptation phase of CRISPR immunity against invading nucleic acids...
