Douglas Losordo

Summary

Affiliation: Northwestern University
Country: USA

Publications

  1. ncbi Intramyocardial transplantation of autologous CD34+ stem cells for intractable angina: a phase I/IIa double-blind, randomized controlled trial
    Douglas W Losordo
    Feinberg Cardiovascular Research Institute, and Department of Medicine, Northwestern University Feinberg School of Medicine and Northwestern Memorial Hospital, Chicago, IL 60611, USA
    Circulation 115:3165-72. 2007
  2. ncbi Sonic hedgehog-modified human CD34+ cells preserve cardiac function after acute myocardial infarction
    Alexander R Mackie
    Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL 60611, USA
    Circ Res 111:312-21. 2012
  3. ncbi Intramyocardial, autologous CD34+ cell therapy for refractory angina
    Douglas W Losordo
    Division of Cardiology, Northwestern Memorial Hospital, Northwestern University, Chicago, IL 60611, USA
    Circ Res 109:428-36. 2011
  4. ncbi IL-10 inhibits inflammation and attenuates left ventricular remodeling after myocardial infarction via activation of STAT3 and suppression of HuR
    Prasanna Krishnamurthy
    Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago IL 60611, USA
    Circ Res 104:e9-18. 2009
  5. ncbi Myocardial knockdown of mRNA-stabilizing protein HuR attenuates post-MI inflammatory response and left ventricular dysfunction in IL-10-null mice
    Prasanna Krishnamurthy
    Feinberg Cardiovascular Research Institute, Division of Cardiology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, USA
    FASEB J 24:2484-94. 2010
  6. ncbi Estradiol triggers sonic-hedgehog-induced angiogenesis during peripheral nerve regeneration by downregulating hedgehog-interacting protein
    Haruki Sekiguchi
    Feinberg Cardiovascular Research Institute and Program in Cardiovascular Regenerative Medicine, Division of Cardiovascular Medicine, Department of Medicine, Northwestern University Feinberg School of Medicine and Northwestern Memorial Hospital, Chicago, IL, USA
    Lab Invest 92:532-42. 2012
  7. ncbi Interleukin-10 deficiency impairs bone marrow-derived endothelial progenitor cell survival and function in ischemic myocardium
    Prasanna Krishnamurthy
    Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago IL 60611, USA
    Circ Res 109:1280-9. 2011
  8. ncbi CXCR4 blockade augments bone marrow progenitor cell recruitment to the neovasculature and reduces mortality after myocardial infarction
    Kentaro Jujo
    Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 107:11008-13. 2010
  9. ncbi Exosomes from human CD34(+) stem cells mediate their proangiogenic paracrine activity
    Susmita Sahoo
    Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL 60611, USA
    Circ Res 109:724-8. 2011
  10. ncbi Biological approaches to ischemic tissue repair: gene- and cell-based strategies
    Jerome Roncalli
    Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Expert Rev Cardiovasc Ther 6:653-68. 2008

Research Grants

  1. Role of Progenitor Cells in Cardiovascular Medicine
    Douglas Losordo; Fiscal Year: 2006
  2. Autologous CD34+ Cell Therapy for Therapeutic Angiogenesis in Advanced PAD
    Douglas Losordo; Fiscal Year: 2007
  3. RESTENOSIS AND ENDOTHELIAL PROLIFERATION
    Douglas Losordo; Fiscal Year: 2007
  4. Progenitor Cells in Vascular Biology
    Douglas Losordo; Fiscal Year: 2007
  5. Role of Progenitor Cells in Cardiovascular Medicine
    Douglas Losordo; Fiscal Year: 2007
  6. Autologous CD34+ Cell Therapy for Therapeutic Angiogenesis in Advanced PAD
    Douglas W Losordo; Fiscal Year: 2010
  7. Role of Progenitor Cells in Cardiovascular Medicine
    Douglas W Losordo; Fiscal Year: 2010
  8. Novel approaches to ischemic tissue repair
    Douglas W Losordo; Fiscal Year: 2010
  9. RESTENOSIS AND ENDOTHELIAL PROLIFERATION
    Douglas Losordo; Fiscal Year: 2002
  10. ESTROGEN, ANGIOGENESIS AND ENDOTHELIAL PROGENITOR CELLS
    Douglas Losordo; Fiscal Year: 2004

Collaborators

Detail Information

Publications35

  1. ncbi Intramyocardial transplantation of autologous CD34+ stem cells for intractable angina: a phase I/IIa double-blind, randomized controlled trial
    Douglas W Losordo
    Feinberg Cardiovascular Research Institute, and Department of Medicine, Northwestern University Feinberg School of Medicine and Northwestern Memorial Hospital, Chicago, IL 60611, USA
    Circulation 115:3165-72. 2007
    ..Preclinical studies have indicated that human CD34+ stem cells induce neovascularization in ischemic myocardium, which enhances perfusion and function...
  2. ncbi Sonic hedgehog-modified human CD34+ cells preserve cardiac function after acute myocardial infarction
    Alexander R Mackie
    Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL 60611, USA
    Circ Res 111:312-21. 2012
    ..Unfortunately, both viability and angiogenic quality of autologous CD34+ cells decline with advanced age and diminished cardiovascular health...
  3. ncbi Intramyocardial, autologous CD34+ cell therapy for refractory angina
    Douglas W Losordo
    Division of Cardiology, Northwestern Memorial Hospital, Northwestern University, Chicago, IL 60611, USA
    Circ Res 109:428-36. 2011
    ..A growing number of patients with coronary disease have refractory angina. Preclinical and early-phase clinical data suggest that intramyocardial injection of autologous CD34+ cells can improve myocardial perfusion and function...
  4. ncbi IL-10 inhibits inflammation and attenuates left ventricular remodeling after myocardial infarction via activation of STAT3 and suppression of HuR
    Prasanna Krishnamurthy
    Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago IL 60611, USA
    Circ Res 104:e9-18. 2009
    ....
  5. ncbi Myocardial knockdown of mRNA-stabilizing protein HuR attenuates post-MI inflammatory response and left ventricular dysfunction in IL-10-null mice
    Prasanna Krishnamurthy
    Feinberg Cardiovascular Research Institute, Division of Cardiology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, USA
    FASEB J 24:2484-94. 2010
    ..Taken together, our studies suggest that HuR is a direct target of IL-10, and HuR knockdown mimics anti-inflammatory effects of IL-10...
  6. ncbi Estradiol triggers sonic-hedgehog-induced angiogenesis during peripheral nerve regeneration by downregulating hedgehog-interacting protein
    Haruki Sekiguchi
    Feinberg Cardiovascular Research Institute and Program in Cardiovascular Regenerative Medicine, Division of Cardiovascular Medicine, Department of Medicine, Northwestern University Feinberg School of Medicine and Northwestern Memorial Hospital, Chicago, IL, USA
    Lab Invest 92:532-42. 2012
    ..Collectively, these findings suggest that E2 improves nerve recovery, at least in part, by reducing HIP expression, which subsequently leads to an increase in Shh signaling and Shh-induced angiogenesis...
  7. ncbi Interleukin-10 deficiency impairs bone marrow-derived endothelial progenitor cell survival and function in ischemic myocardium
    Prasanna Krishnamurthy
    Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago IL 60611, USA
    Circ Res 109:1280-9. 2011
    ....
  8. ncbi CXCR4 blockade augments bone marrow progenitor cell recruitment to the neovasculature and reduces mortality after myocardial infarction
    Kentaro Jujo
    Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 107:11008-13. 2010
    ..Our results indicate that AMD3100 could be a potentially useful therapy for the treatment of myocardial infarction...
  9. ncbi Exosomes from human CD34(+) stem cells mediate their proangiogenic paracrine activity
    Susmita Sahoo
    Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL 60611, USA
    Circ Res 109:724-8. 2011
    ..Previous studies have suggested that paracrine factors secreted by these proangiogenic cells are responsible, at least in part, for the angiogenic effects induced by CD34(+) cell transplantation...
  10. ncbi Biological approaches to ischemic tissue repair: gene- and cell-based strategies
    Jerome Roncalli
    Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Expert Rev Cardiovasc Ther 6:653-68. 2008
    ..This review discusses recent research into both gene and cell therapy and considers the potential application of a combined treatment approach for cardiovascular and cerebrovascular ischemic diseases...
  11. ncbi Endothelial progenitor cells in neovascularization of infarcted myocardium
    Kentaro Jujo
    Feinberg Cardiovascular Research Institute, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA
    J Mol Cell Cardiol 45:530-44. 2008
    ..To optimize therapeutic outcomes, researchers must continue to refine methods of EPC purification, expansion, and administration, and to develop techniques that overcome the intrinsic scarcity and phenotypic deficiencies of EPCs...
  12. ncbi The relative potency and safety of endothelial progenitor cells and unselected mononuclear cells for recovery from myocardial infarction and ischemia
    Haruki Sekiguchi
    Feinberg Cardiovascular Research Institute, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, USA
    J Cell Physiol 219:235-42. 2009
    ..We anticipate that with continued development, EPC therapy will become a safe and effective treatment option for patients with acute myocardial infarction or chronic ischemic disease...
  13. ncbi The Hedgehog transcription factor Gli3 modulates angiogenesis
    Marie Ange Renault
    Feinberg Cardiovascular Research Institute, Northwestern University School of Medicine, 303 E Chicago Ave, Chicago, IL 60611, USA
    Circ Res 105:818-26. 2009
    ....
  14. ncbi Regulation of vascular contractility and blood pressure by the E2F2 transcription factor
    Junlan Zhou
    Assistant Professor, Feinberg Cardiovascular Research Institute, Northwestern University, 303 E Chicago Ave, Tarry 14 751, Chicago, IL 60611, USA
    Circulation 120:1213-21. 2009
    ..E2F proteins are crucially involved in cell-cycle regulation, but their roles in cardiovascular function are poorly understood. Here, we investigated the potential role of E2F2 in blood pressure regulation...
  15. ncbi Notch signaling regulates endothelial progenitor cell activity during recovery from arterial injury in hypercholesterolemic mice
    Masaaki Ii
    Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL 60611, USA
    Circulation 121:1104-12. 2010
    ....
  16. ncbi Sonic hedgehog induces angiogenesis via Rho kinase-dependent signaling in endothelial cells
    Marie Ange Renault
    Feinberg Cardiovascular Research Institute, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
    J Mol Cell Cardiol 49:490-8. 2010
    ..Collectively, our results identify a "nonclassical" pathway by which Shh directly modulates EC phenotype and angiogenic activity...
  17. ncbi CXCR4-mediated bone marrow progenitor cell maintenance and mobilization are modulated by c-kit activity
    Min Cheng
    Feinberg Cardiovascular Research Institute, Northwestern University Feinberg School of Medicine, 303 E Chicago Avenue, Chicago, IL 60611, USA
    Circ Res 107:1083-93. 2010
    ..c-kit, a membrane-bound tyrosine kinase and the receptor for stem cell factor, has also been shown to play a critical role in BM PC mobilization and ischemic tissue repair...
  18. ncbi Cell-free embryonic stem cell extract-mediated derivation of multipotent stem cells from NIH3T3 fibroblasts for functional and anatomical ischemic tissue repair
    Johnson Rajasingh
    Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, 303 E Chicago Ave, Chicago IL 60611, USA
    Circ Res 102:e107-17. 2008
    ..These data provide evidence for the generation of functional multipotent stem-like cells from terminally differentiated somatic cells without the introduction of retroviral mediated transgenes or ESC fusion...
  19. ncbi STAT3-dependent mouse embryonic stem cell differentiation into cardiomyocytes: analysis of molecular signaling and therapeutic efficacy of cardiomyocyte precommitted mES transplantation in a mouse model of myocardial infarction
    Johnson Rajasingh
    Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, 303 E Chicago Ave, Chicago, IL 60611, USA
    Circ Res 101:910-8. 2007
    ..Thus augmentation of LIF/BMP-2 downstream signaling components or cell type specific precommitment may facilitate the effects of ES cell-based therapies for post-MI myocardial repair and regeneration...
  20. ncbi Phase 1/2 placebo-controlled, double-blind, dose-escalating trial of myocardial vascular endothelial growth factor 2 gene transfer by catheter delivery in patients with chronic myocardial ischemia
    Douglas W Losordo
    Division of Cardiology, Tufts School of Medicine St Elizabeth s Medical Center, Boston, Mass 02135, USA
    Circulation 105:2012-8. 2002
    ....
  21. ncbi Intramyocardial transplantation of autologous endothelial progenitor cells for therapeutic neovascularization of myocardial ischemia
    Atsuhiko Kawamoto
    Division of Cardiovascular Research, St Elizabeth's Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 107:461-8. 2003
    ..CONCLUSIONS: These favorable outcomes encourage future clinical trials of catheter-based, intramyocardial transplantation of autologous CD34+ MNCs in the setting of chronic myocardial ischemia...
  22. ncbi One-year follow-up of direct myocardial gene transfer of vascular endothelial growth factor-2 using naked plasmid deoxyribonucleic acid by way of thoracotomy in no-option patients
    F David Fortuin
    Scripps Clinic, Green Hospital, La Jolla, California 92037, USA
    Am J Cardiol 92:436-9. 2003
    ..A randomized phase III trial is underway that will help determine the efficacy of vascular endothelial growth factor-2 gene transfer in "no-option" patients...
  23. ncbi Magnetic resonance mapping of transplanted endothelial progenitor cells for therapeutic neovascularization in ischemic heart disease
    Alberto Weber
    Divisions of Cardiovascular Research and Cardiovascular Medicine, St. Elizabeth's Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA
    Eur J Cardiothorac Surg 26:137-43. 2004
    ..CONCLUSIONS: Magnetically labeled EPCs transplanted for therapeutic neovascularization in myocardial ischemia can be visualized with ex vivo MRI at high-field strengths...
  24. ncbi Local gene transfer of phVEGF-2 plasmid by gene-eluting stents: an alternative strategy for inhibition of restenosis
    Dirk H Walter
    Department of Medicine, St Elizabeth's Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 110:36-45. 2004
    ..VEGF-2 gene-eluting stents may be considered as a stand-alone or combination therapy...
  25. ncbi Mending the broken heart: ischemic preconditioning-stimulated myocardial angiogenesis
    Raj Kishore
    J Mol Cell Cardiol 37:1-3. 2004
  26. ncbi Synergistic effect of bone marrow mobilization and vascular endothelial growth factor-2 gene therapy in myocardial ischemia
    Atsuhiko Kawamoto
    Division of Cardiovascular Research, St Elizabeth's Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 110:1398-405. 2004
    ..CONCLUSIONS: Mobilization of endothelial progenitor cells with cytokines potentiates VEGF-2 gene therapy for myocardial ischemia and enhances bone marrow cell incorporation into ischemic myocardium...
  27. ncbi Clonally expanded novel multipotent stem cells from human bone marrow regenerate myocardium after myocardial infarction
    Young Sup Yoon
    Division of Cardiovascular Medicine, Caritas St Elizabeth s Medical Center, Boston, Massachusetts 02135, USA
    J Clin Invest 115:326-38. 2005
    ..To our knowledge, this is the first demonstration that a specific population of multipotent human BM-derived stem cells can induce both therapeutic neovascularization and endogenous and exogenous cardiomyogenesis...
  28. ncbi Endothelial progenitor cells are rapidly recruited to myocardium and mediate protective effect of ischemic preconditioning via "imported" nitric oxide synthase activity
    Masaaki Ii
    Division of Cardiovascular Research, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 111:1114-20. 2005
    ..The possibility that EPCs could act as reservoirs of cytokines has been implied by several observations; however, a specific role for cytokine delivery has not been identified...
  29. ncbi Long-term (2-year) clinical events following transthoracic intramyocardial gene transfer of VEGF-2 in no-option patients
    John P Reilly
    Scripps Clinic, Interventional Cardiology, La Jolla, California, USA
    J Interv Cardiol 18:27-31. 2005
    ..The majority of events were the result of progression of disease in areas of the heart remote from the site of GT. A large randomized trial is planned to determine the efficacy of intramyocardial VEGF-2 injections in inoperable patients...
  30. ncbi Progressive attenuation of myocardial vascular endothelial growth factor expression is a seminal event in diabetic cardiomyopathy: restoration of microvascular homeostasis and recovery of cardiac function in diabetic cardiomyopathy after replenishment of
    Young Sup Yoon
    Division of Cardiovascular Research, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 111:2073-85. 2005
    ..Diabetic cardiomyopathy (DCM) is characterized by microvascular pathology and interstitial fibrosis, which leads to progressive heart failure; however, the pathogenesis of DCM remains uncertain...
  31. ncbi Sonic hedgehog myocardial gene therapy: tissue repair through transient reconstitution of embryonic signaling
    Kengo F Kusano
    Division of Cardiovascular Research, St Elizabeth Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02135, USA
    Nat Med 11:1197-204. 2005
    ....
  32. ncbi Synergistic effect of combined intramyocardial CD34+ cells and VEGF2 gene therapy after MI
    Satoshi Shintani
    Division of Cardiovascular Research, Caritas St Elizabeth's Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA
    Nat Clin Pract Cardiovasc Med 3:S123-8. 2006
    ..This approach may overcome therapeutic failures (e.g. inability of certain patients to mobilize sufficient EPCs) and may also offer safety advantages by allowing lower dosing strategies...
  33. ncbi CD34-positive cells exhibit increased potency and safety for therapeutic neovascularization after myocardial infarction compared with total mononuclear cells
    Atsuhiko Kawamoto
    Division of Cardiovascular Research, St Elizabeth's Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA
    Circulation 114:2163-9. 2006
    ..4+/-0.4, 24.9+/-0.4, and 24.1+/-0.6; P<0.05). CONCLUSIONS: CD34+ cells exhibit superior efficacy for preserving myocardial integrity and function after MI than unselected circulating MNCs...
  34. ncbi Long-term stable expression of human growth hormone by rAAV promotes myocardial protection post-myocardial infarction
    Kengo Kusano
    Cardiovascular Research, Caritas St Elizabeth s Medical Center, Tufts University School of Medicine, 736 Cambridge St, Boston, MA 02135, USA
    J Mol Cell Cardiol 42:390-9. 2007
    ....
  35. ncbi Acetylcysteine to prevent angiography-related renal tissue injury (the APART trial)
    Larry J Diaz-Sandoval
    Division of Cardiovascular Medicine, St. Elizabeth's Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02135, USA
    Am J Cardiol 89:356-8. 2002

Research Grants36

  1. Role of Progenitor Cells in Cardiovascular Medicine
    Douglas Losordo; Fiscal Year: 2006
    ..Accordingly, in this proposal we will employ a series of in vivo and in vitro model systems to define the role of EPCs in recovery from myocardial ischemia with a goal of developing future therapeutic strategies exploiting these cells. ..
  2. Autologous CD34+ Cell Therapy for Therapeutic Angiogenesis in Advanced PAD
    Douglas Losordo; Fiscal Year: 2007
    ....
  3. RESTENOSIS AND ENDOTHELIAL PROLIFERATION
    Douglas Losordo; Fiscal Year: 2007
    ..Together these studies will result in a better understanding of the mechanisms, which govern EC recovery at sites of balloon injury and may lead to novel therapeutic strategies targeting endothelial recovery. ..
  4. Progenitor Cells in Vascular Biology
    Douglas Losordo; Fiscal Year: 2007
    ..The ultimate goal of these studies is to identify future clinical strategies for tissue repair. ..
  5. Role of Progenitor Cells in Cardiovascular Medicine
    Douglas Losordo; Fiscal Year: 2007
    ..abstract_text> ..
  6. Autologous CD34+ Cell Therapy for Therapeutic Angiogenesis in Advanced PAD
    Douglas W Losordo; Fiscal Year: 2010
    ....
  7. Role of Progenitor Cells in Cardiovascular Medicine
    Douglas W Losordo; Fiscal Year: 2010
    ....
  8. Novel approaches to ischemic tissue repair
    Douglas W Losordo; Fiscal Year: 2010
    ....
  9. RESTENOSIS AND ENDOTHELIAL PROLIFERATION
    Douglas Losordo; Fiscal Year: 2002
    ....
  10. ESTROGEN, ANGIOGENESIS AND ENDOTHELIAL PROGENITOR CELLS
    Douglas Losordo; Fiscal Year: 2004
    ..abstract_text> ..
  11. THERAPEUTIC ANGIOGENESIS IN VASCULAR MEDICINE II
    Douglas Losordo; Fiscal Year: 2004
    ..abstract_text> ..
  12. RE-ENDOTHELIALIZATION AND RESTENOSIS II
    Douglas Losordo; Fiscal Year: 2004
    ..Specific Aim 3 is organized to explore the impact of EC mitogens, EC antagonists, and genetic engineering on EPC incorporation into neoendothelium. ..
  13. Therapeutic Angiogenesis in Vascular Medicine III
    Douglas W Losordo; Fiscal Year: 2010
    ....