Michael C Jewett

Summary

Affiliation: Northwestern University
Country: USA

Publications

  1. pmc In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation
    Michael C Jewett
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Syst Biol 9:678. 2013
  2. pmc Update on designing and building minimal cells
    Michael C Jewett
    Department of Chemical and Biological Engineering and Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Biotechnol 21:697-703. 2010
  3. pmc The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Nucleic Acids Res 42:6774-85. 2014
  4. doi request reprint Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis
    C Eric Hodgman
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Biotechnol Bioeng 110:2643-54. 2013
  5. pmc Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1
    Seok Hoon Hong
    Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Tech E 136, Evanston, IL 60208 USA
    Chembiochem 16:844-53. 2015
  6. doi request reprint Characterizing and Alleviating Substrate Limitations for Improved in vitro Ribosome Construction
    Yi Liu
    Interdepartmental Biological Sciences Graduate Program, Chemistry of Life Processes Institute, Department of Chemical and Biological Engineering, Member, Robert H Lurie Comprehensive Cancer Center, Affiliate Member, Institute for BioNanotechnology in Medicine, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States
    ACS Synth Biol 4:454-62. 2015
  7. pmc Biology by design: from top to bottom and back
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    J Biomed Biotechnol 2010:232016. 2010
  8. pmc Energizing eukaryotic cell-free protein synthesis with glucose metabolism
    Mark J Anderson
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Technological Institute E136, Evanston, IL 60208 3120, USA Chemistry of Life Processes Institute, Northwestern University, 2170 Campus Drive, Evanston, IL 60208 3120, USA
    FEBS Lett 589:1723-7. 2015
  9. pmc Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Nucleic Acids Res 43:4774-84. 2015
  10. doi request reprint Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis
    C Eric Hodgman
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Technological Institute, E136, Evanston, IL 60208 3120, USA Chemistry of Life Processes Institute, Northwestern University, 2170 Campus Drive, Evanston, IL 60208 3120, USA
    N Biotechnol 31:499-505. 2014

Collaborators

Detail Information

Publications17

  1. pmc In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation
    Michael C Jewett
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Syst Biol 9:678. 2013
    ..We anticipate that iSAT will aid studies of ribosome assembly and open new avenues for making ribosomes with altered properties. ..
  2. pmc Update on designing and building minimal cells
    Michael C Jewett
    Department of Chemical and Biological Engineering and Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Biotechnol 21:697-703. 2010
    ..Major progress in the past 5 years has occurred in synthetic genomics, minimization of the Escherichia coli genome, sequencing of minimal bacterial endosymbionts, identification of essential genes, and integration of biochemical systems...
  3. pmc The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Nucleic Acids Res 42:6774-85. 2014
    ..coli cells. Looking forward, we believe iSAT will enable unique studies to unravel the systems biology of ribosome biogenesis and open the way to new methods for making and studying ribosomal variants. ..
  4. doi request reprint Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis
    C Eric Hodgman
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Biotechnol Bioeng 110:2643-54. 2013
    ..Our results set the stage for developing a yeast CFPS platform that provides for high-yielding and cost-effective expression of a variety of protein therapeutics and protein libraries...
  5. pmc Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1
    Seok Hoon Hong
    Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Tech E 136, Evanston, IL 60208 USA
    Chembiochem 16:844-53. 2015
    ..Our work has implications for using whole genome editing for CFPS strain development, expanding the chemistry of biological systems, and cell-free synthetic biology...
  6. doi request reprint Characterizing and Alleviating Substrate Limitations for Improved in vitro Ribosome Construction
    Yi Liu
    Interdepartmental Biological Sciences Graduate Program, Chemistry of Life Processes Institute, Department of Chemical and Biological Engineering, Member, Robert H Lurie Comprehensive Cancer Center, Affiliate Member, Institute for BioNanotechnology in Medicine, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States
    ACS Synth Biol 4:454-62. 2015
    ..Our results underscore the critical role energy substrates play in iSAT and highlight the importance of understanding metabolic processes that influence substrate depletion for cell-free synthetic biology. ..
  7. pmc Biology by design: from top to bottom and back
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    J Biomed Biotechnol 2010:232016. 2010
    ..Ten years after the construction of synthetic biology's first devices, the drive to look beyond what does exist to what can exist is ushering in an era of biology by design...
  8. pmc Energizing eukaryotic cell-free protein synthesis with glucose metabolism
    Mark J Anderson
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Technological Institute E136, Evanston, IL 60208 3120, USA Chemistry of Life Processes Institute, Northwestern University, 2170 Campus Drive, Evanston, IL 60208 3120, USA
    FEBS Lett 589:1723-7. 2015
    ..Our demonstration provides the foundation for development of cost-effective eukaryotic CFPS platforms...
  9. pmc Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Nucleic Acids Res 43:4774-84. 2015
    ....
  10. doi request reprint Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis
    C Eric Hodgman
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Technological Institute, E136, Evanston, IL 60208 3120, USA Chemistry of Life Processes Institute, Northwestern University, 2170 Campus Drive, Evanston, IL 60208 3120, USA
    N Biotechnol 31:499-505. 2014
    ..This study highlights the crucial role translation initiation plays in yeast CFPS and offers a simple platform to study IRES sequences...
  11. pmc Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation
    Seok Hoon Hong
    Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Department of Chemistry, and Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, United States of America
    ACS Synth Biol 3:398-409. 2014
    ..Looking forward, using RF1-deficient strains for extract-based CFPS will aid in the synthesis of proteins and biopolymers with site-specifically incorporated NSAAs...
  12. doi request reprint A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthe
    Rui Gan
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
    Biotechnol J 9:641-51. 2014
    ..See accompanying commentary by Russ and Dueber DOI: 10.1002/biot.201400071. ..
  13. doi request reprint Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis
    Jennifer A Schoborg
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA
    Biotechnol J 9:630-40. 2014
    ..This study identifies targets for the future improvement of the batch yeast CFPS reaction. Moreover, it outlines a detailed, generalized method to characterize and improve other CFPS platforms. ..
  14. doi request reprint Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
    Farren J Isaacs
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 333:348-53. 2011
    ..Our methods treat the chromosome as both an editable and an evolvable template, permitting the exploration of vast genetic landscapes...
  15. pmc Synthetic in vitro circuits
    Adam J Hockenberry
    Interdepartmental Biological Sciences Graduate Program, Northwestern University, Evanston, IL 60208, USA
    Curr Opin Chem Biol 16:253-9. 2012
    ..Progress in this field is showing that cell-free synthetic biology is a versatile testing ground for understanding native biological circuits and engineering novel functionality...
  16. pmc Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry
    D Calvin Harris
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Biotechnol 23:672-8. 2012
    ..In the coming years, the impact of advances inspired by these approaches will make possible the synthesis of nonbiological polymers having new backbone compositions, new chemical properties, new structures, and new functions...
  17. pmc Cell-free protein synthesis: applications come of age
    Erik D Carlson
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Biotechnol Adv 30:1185-94. 2012
    ..In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications...