Michael C Jewett

Summary

Affiliation: Northwestern University
Country: USA

Publications

  1. pmc In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation
    Michael C Jewett
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Syst Biol 9:678. 2013
  2. pmc Update on designing and building minimal cells
    Michael C Jewett
    Department of Chemical and Biological Engineering and Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Biotechnol 21:697-703. 2010
  3. pmc The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Nucleic Acids Res 42:6774-85. 2014
  4. doi request reprint Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis
    C Eric Hodgman
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Biotechnol Bioeng 110:2643-54. 2013
  5. pmc Biology by design: from top to bottom and back
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    J Biomed Biotechnol 2010:232016. 2010
  6. pmc Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation
    Seok Hoon Hong
    Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Department of Chemistry, and Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, United States of America
    ACS Synth Biol 3:398-409. 2014
  7. ncbi request reprint Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis
    Jennifer A Schoborg
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA
    Biotechnol J 9:630-40. 2014
  8. ncbi request reprint A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthe
    Rui Gan
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
    Biotechnol J 9:641-51. 2014
  9. ncbi request reprint Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
    Farren J Isaacs
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 333:348-53. 2011
  10. pmc Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry
    D Calvin Harris
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Biotechnol 23:672-8. 2012

Collaborators

Detail Information

Publications12

  1. pmc In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation
    Michael C Jewett
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Syst Biol 9:678. 2013
    ..We anticipate that iSAT will aid studies of ribosome assembly and open new avenues for making ribosomes with altered properties. ..
  2. pmc Update on designing and building minimal cells
    Michael C Jewett
    Department of Chemical and Biological Engineering and Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Biotechnol 21:697-703. 2010
    ..Major progress in the past 5 years has occurred in synthetic genomics, minimization of the Escherichia coli genome, sequencing of minimal bacterial endosymbionts, identification of essential genes, and integration of biochemical systems...
  3. pmc The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Nucleic Acids Res 42:6774-85. 2014
    ..coli cells. Looking forward, we believe iSAT will enable unique studies to unravel the systems biology of ribosome biogenesis and open the way to new methods for making and studying ribosomal variants. ..
  4. doi request reprint Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis
    C Eric Hodgman
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Biotechnol Bioeng 110:2643-54. 2013
    ..Our results set the stage for developing a yeast CFPS platform that provides for high-yielding and cost-effective expression of a variety of protein therapeutics and protein libraries...
  5. pmc Biology by design: from top to bottom and back
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    J Biomed Biotechnol 2010:232016. 2010
    ..Ten years after the construction of synthetic biology's first devices, the drive to look beyond what does exist to what can exist is ushering in an era of biology by design...
  6. pmc Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation
    Seok Hoon Hong
    Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Department of Chemistry, and Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, United States of America
    ACS Synth Biol 3:398-409. 2014
    ..Looking forward, using RF1-deficient strains for extract-based CFPS will aid in the synthesis of proteins and biopolymers with site-specifically incorporated NSAAs...
  7. ncbi request reprint Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis
    Jennifer A Schoborg
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA
    Biotechnol J 9:630-40. 2014
    ..This study identifies targets for the future improvement of the batch yeast CFPS reaction. Moreover, it outlines a detailed, generalized method to characterize and improve other CFPS platforms. ..
  8. ncbi request reprint A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthe
    Rui Gan
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
    Biotechnol J 9:641-51. 2014
    ..See accompanying commentary by Russ and Dueber DOI: 10.1002/biot.201400071. ..
  9. ncbi request reprint Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
    Farren J Isaacs
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 333:348-53. 2011
    ..Our methods treat the chromosome as both an editable and an evolvable template, permitting the exploration of vast genetic landscapes...
  10. pmc Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry
    D Calvin Harris
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Biotechnol 23:672-8. 2012
    ..In the coming years, the impact of advances inspired by these approaches will make possible the synthesis of nonbiological polymers having new backbone compositions, new chemical properties, new structures, and new functions...
  11. pmc Synthetic in vitro circuits
    Adam J Hockenberry
    Interdepartmental Biological Sciences Graduate Program, Northwestern University, Evanston, IL 60208, USA
    Curr Opin Chem Biol 16:253-9. 2012
    ..Progress in this field is showing that cell-free synthetic biology is a versatile testing ground for understanding native biological circuits and engineering novel functionality...
  12. pmc Cell-free protein synthesis: applications come of age
    Erik D Carlson
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Biotechnol Adv 30:1185-94. 2012
    ..In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications...