Nicholas Cianciotto

Summary

Affiliation: Northwestern University
Country: USA

Publications

  1. ncbi request reprint Pathogenicity of Legionella pneumophila
    N P Cianciotto
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Int J Med Microbiol 291:331-43. 2001
  2. ncbi request reprint Iron acquisition by Legionella pneumophila
    Nicholas P Cianciotto
    Department of Microbiology Immunology, Northwestern University Medical School, 320 East Superior Street, Chicago, IL 60611 3010, USA
    Biometals 20:323-31. 2007
  3. pmc Many substrates and functions of type II secretion: lessons learned from Legionella pneumophila
    Nicholas P Cianciotto
    Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, IL 60611, USA
    Future Microbiol 4:797-805. 2009
  4. ncbi request reprint Impact of the bacterial type I cytochrome c maturation system on different biological processes
    Nicholas P Cianciotto
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Mol Microbiol 56:1408-15. 2005
  5. ncbi request reprint Type II secretion: a protein secretion system for all seasons
    Nicholas P Cianciotto
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Trends Microbiol 13:581-8. 2005
  6. pmc lbtA and lbtB are required for production of the Legionella pneumophila siderophore legiobactin
    Kimberly A Allard
    Department of Microbiology Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, Illinois 60611 3010, USA
    J Bacteriol 188:1351-63. 2006
  7. pmc The Legionella pneumophila tatB gene facilitates secretion of phospholipase C, growth under iron-limiting conditions, and intracellular infection
    Ombeline Rossier
    Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, IL 60611, USA
    Infect Immun 73:2020-32. 2005
  8. pmc Legionella pneumophila feoAB promotes ferrous iron uptake and intracellular infection
    Marianne Robey
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Infect Immun 70:5659-69. 2002
  9. pmc Purification of Legiobactin and importance of this siderophore in lung infection by Legionella pneumophila
    Kimberly A Allard
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Infect Immun 77:2887-95. 2009
  10. ncbi request reprint Cytochrome c maturation proteins are critical for in vivo growth of Legionella pneumophila
    Jennifer Naylor
    Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, IL 60611, USA
    FEMS Microbiol Lett 241:249-56. 2004

Research Grants

  1. GENETICS OF IRON ACQUISITION BY LEGIONELLA PNEUMOPHILA
    Nicholas Cianciotto; Fiscal Year: 2007
  2. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas Cianciotto; Fiscal Year: 2007
  3. GENETICS OF IRON ACQUISITION BY LEGIONELLA PNEUMOPHILA
    Nicholas Cianciotto; Fiscal Year: 2009
  4. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas Cianciotto; Fiscal Year: 2009
  5. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas P Cianciotto; Fiscal Year: 2010
  6. GENETICS OF IRON ACQUISITION BY LEGIONELLA PNEUMOPHILA
    Nicholas P Cianciotto; Fiscal Year: 2010
  7. Sixth International Conference on Legionella
    Nicholas Cianciotto; Fiscal Year: 2005
  8. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas Cianciotto; Fiscal Year: 2003
  9. GENETICS OF IRON ACQUISITION BY LEGIONELLA PNEUMOPHILA
    Nicholas Cianciotto; Fiscal Year: 2003
  10. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas P Cianciotto; Fiscal Year: 2011

Collaborators

  • Shawn R Starkenburg
  • Antje Flieger
  • P Cornelis
  • Carmen Buchrieser
  • Ombeline Rossier
  • Jenny Dao
  • Maria A Söderberg
  • Catherine R Stewart
  • Christa H Chatfield
  • Hayley J Newton
  • Sruti DebRoy
  • Kimberly A Allard
  • Fiona M Sansom
  • Kessler McCoy-Simandle
  • Elizabeth L Hartland
  • Jennifer Naylor
  • Denise M Burnside
  • V K Viswanathan
  • Virginia Aragon
  • Meghan M Pearce
  • Wilhelmina M Huston
  • Christopher T Lemke
  • Sangeeta Banerji
  • Sherry Kurtz
  • Iouri Belyi
  • Marianne Robey
  • Steven D Zink
  • Yousef Abu-Kwaik
  • Maelle Molmeret
  • Brendan J Mulhern
  • Paul J Bryce
  • David S Crumrine
  • Domenic Castignetti
  • Prakash Sanjeevaiah
  • Holger Bruggemann
  • Christiane Albert-Weissenberger
  • Alastair G McEwan
  • Christel Cazalet
  • Michael P Jennings
  • Adrian D McAlister
  • Anthony J F d'Apice
  • Peter J Cowan
  • Sandra Crikis
  • Joan Sloan
  • Maria Söderberg
  • Albert M Berghuis
  • Mayte Bewersdorff
  • Björn Hermes
  • Jiyoung Hwang
  • Bing Xiong
  • Michel R Popoff
  • O A Terry Alli
  • Steven Zink
  • Lisa Pedersen
  • Sejal Mody
  • Yousef Abu Kwaik
  • Kevin Krcmarik
  • Lisa L Pedersen

Detail Information

Publications38

  1. ncbi request reprint Pathogenicity of Legionella pneumophila
    N P Cianciotto
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Int J Med Microbiol 291:331-43. 2001
    ..The stationary phase response and the iron acquisition functions of L. pneumophila also play key roles in pathogenesis, as do a number of other loci, including the pts, mil and enh genes...
  2. ncbi request reprint Iron acquisition by Legionella pneumophila
    Nicholas P Cianciotto
    Department of Microbiology Immunology, Northwestern University Medical School, 320 East Superior Street, Chicago, IL 60611 3010, USA
    Biometals 20:323-31. 2007
    ..Some of our other studies have discovered a critical, yet undefined, role for the L. pneumophila cytochrome c maturation locus in low-iron growth, intracellular infection, and virulence...
  3. pmc Many substrates and functions of type II secretion: lessons learned from Legionella pneumophila
    Nicholas P Cianciotto
    Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, IL 60611, USA
    Future Microbiol 4:797-805. 2009
    ..In this context, type II secretion represents a potentially important target for industrial and biomedical applications...
  4. ncbi request reprint Impact of the bacterial type I cytochrome c maturation system on different biological processes
    Nicholas P Cianciotto
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Mol Microbiol 56:1408-15. 2005
    ....
  5. ncbi request reprint Type II secretion: a protein secretion system for all seasons
    Nicholas P Cianciotto
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Trends Microbiol 13:581-8. 2005
    ..Thus, it is an opportune time to highlight the new and different ways in which T2S serves bacterial function...
  6. pmc lbtA and lbtB are required for production of the Legionella pneumophila siderophore legiobactin
    Kimberly A Allard
    Department of Microbiology Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, Illinois 60611 3010, USA
    J Bacteriol 188:1351-63. 2006
    ..The discovery of lbtAB represents the first identification of genes required for L. pneumophila siderophore expression...
  7. pmc The Legionella pneumophila tatB gene facilitates secretion of phospholipase C, growth under iron-limiting conditions, and intracellular infection
    Ombeline Rossier
    Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, IL 60611, USA
    Infect Immun 73:2020-32. 2005
    ..Thus, L. pneumophila tatB plays a role in the formation of a respiratory complex, growth under low-iron conditions, the secretion of a phospholipase C activity, and intracellular infection...
  8. pmc Legionella pneumophila feoAB promotes ferrous iron uptake and intracellular infection
    Marianne Robey
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Infect Immun 70:5659-69. 2002
    ..These data represent the first evidence for the importance of ferrous iron transport for intracellular replication by a human pathogen...
  9. pmc Purification of Legiobactin and importance of this siderophore in lung infection by Legionella pneumophila
    Kimberly A Allard
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Infect Immun 77:2887-95. 2009
    ..Overall, these data represent the first documentation of a role for siderophore expression in the virulence of L. pneumophila...
  10. ncbi request reprint Cytochrome c maturation proteins are critical for in vivo growth of Legionella pneumophila
    Jennifer Naylor
    Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, IL 60611, USA
    FEMS Microbiol Lett 241:249-56. 2004
    ..Taken together, these data confirm that ccm and cytochrome c maturation proteins are required for L. pneumophila growth in low iron, intracellular infection, and virulence...
  11. pmc Legionella pneumophila Mip, a surface-exposed peptidylproline cis-trans-isomerase, promotes the presence of phospholipase C-like activity in culture supernatants
    Sruti DebRoy
    Department of Microbiology Immunology, Northwestern University Medical School, 320 East Superior Street, Chicago, IL 60611 3010, USA
    Infect Immun 74:5152-60. 2006
    ..These data provide both the first evidence for a target for Mip and the first indication that a surface PPIase is involved in the secretion or activation of proteins beyond the outer membrane...
  12. pmc Characterization of the gene encoding the major secreted lysophospholipase A of Legionella pneumophila and its role in detoxification of lysophosphatidylcholine
    Antje Flieger
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, Germany
    Infect Immun 70:6094-106. 2002
    ..pneumophila. In the course of characterizing plaA, we discovered that wild-type legionellae secrete a phospholipid cholesterol acyltransferase activity, highlighting the spectrum of lipolytic enzymes produced by L. pneumophila...
  13. pmc The cytochrome c maturation locus of Legionella pneumophila promotes iron assimilation and intracellular infection and contains a strain-specific insertion sequence element
    V K Viswanathan
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Infect Immun 70:1842-52. 2002
    ..pneumophila but was absent from others. These findings represent the first evidence for a transposable element in Legionella and the first identification of an L. pneumophila strain-specific gene...
  14. pmc icmT is essential for pore formation-mediated egress of Legionella pneumophila from mammalian and protozoan cells
    Maelle Molmeret
    Department of Microbiology and Immunology, The University of Kentucky College of Medicine, Lexington, Kentucky 40536 0084, USA
    Infect Immun 70:69-78. 2002
    ..We conclude that the icmT gene is essential for pore formation-mediated lysis of mammalian and protozoan cells and the subsequent bacterial egress...
  15. pmc The type II secretion system of Legionella pneumophila elaborates two aminopeptidases, as well as a metalloprotease that contributes to differential infection among protozoan hosts
    Ombeline Rossier
    Department of Microbiology Immunology, Northwestern University Medical School, 320 East Superior Ave, Chicago, IL 60611 3010, USA
    Appl Environ Microbiol 74:753-61. 2008
    ..pneumophila gene that shows differential importance among protozoan infection models, suggesting that the legionellae might have evolved some of its factors to especially target certain of their protozoan hosts...
  16. pmc Surface translocation by Legionella pneumophila: a form of sliding motility that is dependent upon type II protein secretion
    Catherine R Stewart
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    J Bacteriol 191:1537-46. 2009
    ..Together, these data indicate that L. pneumophila exhibits a form of surface translocation that is most akin to "sliding motility" and uniquely dependent upon type II secretion...
  17. pmc The secreted pyomelanin pigment of Legionella pneumophila confers ferric reductase activity
    Christa H Chatfield
    Department of Microbiology Immunology, Northwestern University Medical School, 320 East Superior Street, Chicago, IL 60611 3010, USA
    Infect Immun 75:4062-70. 2007
    ..Together, these data represent the first demonstration of pyomelanin-mediated ferric reduction by a pathogenic bacterium...
  18. pmc A type II secreted RNase of Legionella pneumophila facilitates optimal intracellular infection of Hartmannella vermiformis
    Ombeline Rossier
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Microbiology 155:882-90. 2009
    ..pneumophila type II secretion mutants are due to the loss of multiple secreted effectors...
  19. pmc Importance of type II secretion for survival of Legionella pneumophila in tap water and in amoebae at low temperatures
    Maria A Söderberg
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Appl Environ Microbiol 74:5583-8. 2008
    ..There was a correlation between low-temperature survival and secretion function when 12 additional Legionella species were examined...
  20. pmc Legionella pneumophila type II secretome reveals unique exoproteins and a chitinase that promotes bacterial persistence in the lung
    Sruti DebRoy
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 103:19146-51. 2006
    ..Our data also indicate that an enzyme with chitinase activity can promote infection of a mammalian host...
  21. pmc Legionella pneumophila type II protein secretion promotes virulence in the A/J mouse model of Legionnaires' disease pneumonia
    Ombeline Rossier
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Infect Immun 72:310-21. 2004
    ..Examination of infected mouse sera revealed that type II secreted proteins are expressed in vivo. Thus, the L. pneumophila Lsp system is a virulence factor and the only type II secretion system linked to intracellular infection...
  22. pmc The Dot/Icm type IV secretion system of Legionella pneumophila is essential for the induction of apoptosis in human macrophages
    Steven D Zink
    Department of Microbiology and Immunology, University of Kentucky Chandler Medical Center, Lexington, Kentucky 40536 0084, USA
    Infect Immun 70:1657-63. 2002
    ..pneumophila to induce apoptosis. Our data provide the first example of the role of a type IV secretion system of a bacterial pathogen in the induction of apoptosis in the host cell...
  23. pmc The type II protein secretion system of Legionella pneumophila promotes growth at low temperatures
    Maria A Söderberg
    Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, IL 60611, USA
    J Bacteriol 186:3712-20. 2004
    ..In summary, these data document, for the first time, that L. pneumophila replicates at temperatures below 20 degrees C and that a bacterial type II protein secretion system facilitates growth at low temperatures...
  24. pmc The surfactant of Legionella pneumophila Is secreted in a TolC-dependent manner and is antagonistic toward other Legionella species
    Catherine R Stewart
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611 3010, USA
    J Bacteriol 193:5971-84. 2011
    ..These data suggest that the surfactant provides L. pneumophila with a selective advantage over other legionellae in the natural environment...
  25. pmc Legionella pneumophila type II secretion dampens the cytokine response of infected macrophages and epithelia
    Kessler McCoy-Simandle
    Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, IL 60611, USA
    Infect Immun 79:1984-97. 2011
    ..To our knowledge, these data are the first to identify a link between a T2S system and the modulation of immune factors following intracellular infection...
  26. pmc Legionella pneumophila LbtU acts as a novel, TonB-independent receptor for the legiobactin siderophore
    Christa H Chatfield
    Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, IL 60611, USA
    J Bacteriol 193:1563-75. 2011
    ..This observation coupled with the fact that L. pneumophila does not encode TonB suggests that LbtU is a new type of receptor that participates in a form of iron uptake that is mechanistically distinct from the existing paradigm...
  27. ncbi request reprint Siderophore activity among members of the Legionella genus
    Shawn R Starkenburg
    Department of Microbiology Immunology, Northwestern University Medical School, 320 East Superior Street, Chicago, IL 60611, USA
    Curr Microbiol 49:203-7. 2004
    ..Although some of the species did not grow in CDM, the majority replicated and secreted CAS reactivity, suggesting that siderophores are conserved among the legionellae...
  28. pmc Legionella pneumophila secretes an endoglucanase that belongs to the family-5 of glycosyl hydrolases and is dependent upon type II secretion
    Meghan M Pearce
    Department of Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St, Chicago, IL 60611, USA
    FEMS Microbiol Lett 300:256-64. 2009
    ..Overall, this study represents the first documentation of an endoglucanase (EC 3.2.1.4) being produced by a strain of Legionella...
  29. pmc Mediators of lipid A modification, RNA degradation, and central intermediary metabolism facilitate the growth of Legionella pneumophila at low temperatures
    Maria A Söderberg
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Curr Microbiol 60:59-65. 2010
    ..Thus, L. pneumophila not only employs some of the same processes and factors as other bacteria do in order to survive at low temperatures (e.g., LpxP, CsdA), but it also appears to possess novel modes of cold adaptation...
  30. ncbi request reprint Legionella pneumophila genes that encode lipase and phospholipase C activities
    Virginia Aragon
    Department of Microbiology Immunology, Northwestern University Medical School, 320 East Superior Street, Chicago, IL 60611 3010, USA
    Microbiology 148:2223-31. 2002
    ..Taken together, these data indicate that L. pneumophila has multiple lipases and possibly several phospholipase C enzymes but that LipA, LipB and PlcA are not among those exoproteins required for optimal intracellular infection...
  31. pmc A Legionella pneumophila peptidyl-prolyl cis-trans isomerase present in culture supernatants is necessary for optimal growth at low temperatures
    Maria A Söderberg
    Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Appl Environ Microbiol 74:1634-8. 2008
    ..Mutants lacking ppiB exhibited reduced growth at 17 degrees C. Since PpiB lacked a signal sequence and was present in 17 degrees C supernatants of type II and type IV secretion mutants, this protein may be secreted by a novel mechanism...
  32. ncbi request reprint A bacterial ecto-triphosphate diphosphohydrolase similar to human CD39 is essential for intracellular multiplication of Legionella pneumophila
    Fiona M Sansom
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria 3800, Australia
    Cell Microbiol 9:1922-35. 2007
    ..Although several protozoan parasites exhibit ecto-NTPDase activity, including Toxoplasma gondii, Trichomonas vaginalis and Trypanosoma cruzi, this is the first time a bacterial ecto-NTPDase has been implicated in virulence...
  33. pmc Sel1 repeat protein LpnE is a Legionella pneumophila virulence determinant that influences vacuolar trafficking
    Hayley J Newton
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria 3800, Australia
    Infect Immun 75:5575-85. 2007
    ..This investigation has further characterized the contribution of LpnE to L. pneumophila virulence and, more specifically, the importance of the SLR regions to LpnE function...
  34. doi request reprint Functional analysis of the multi-copper oxidase from Legionella pneumophila
    Wilhelmina M Huston
    Centre for Metals in Biology and School of Molecular and Microbial Sciences, University of Queensland, St Lucia, QLD 4072, Australia
    Microbes Infect 10:497-503. 2008
    ..However, a function in high-affinity acquisition of iron could also be possible given the inability of the McoL mutants to grow aerobically under iron-limiting conditions...
  35. pmc Purification and characterization of a UDP-glucosyltransferase produced by Legionella pneumophila
    Iouri Belyi
    Gamaleya Research Institute of Epidemiology and Microbiology, Moscow 123098, Russia
    Infect Immun 71:181-6. 2003
    ..pneumophila proliferation...
  36. pmc Characterization of the major secreted zinc metalloprotease- dependent glycerophospholipid:cholesterol acyltransferase, PlaC, of Legionella pneumophila
    Sangeeta Banerji
    Robert Koch Institut, Research Group NG5 Pathogenesis of Legionella Infections, Nordufer 20, D 13353 Berlin, Germany
    Infect Immun 73:2899-909. 2005
    ..Cholesterol is an important compound of eukaryotic membranes, and an acyltransferase might be a tool for host cell modification to fit the needs of the bacterium...
  37. pmc Significant role for ladC in initiation of Legionella pneumophila infection
    Hayley J Newton
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria 3800, Australia
    Infect Immun 76:3075-85. 2008
    ..castellanii infection. Although the mechanism by which LadC modulates the initial interaction between the bacterium and host cell remains unclear, we have established that LadC plays an important role in L. pneumophila infection...
  38. pmc Crystallization and preliminary crystallographic analysis of an aminoglycoside kinase from Legionella pneumophila
    Christopher T Lemke
    Department of Microbiology and Immunology, McGill University, Canada
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:606-8. 2005
    ..Here, the crystallization and preliminary X-ray studies of this enzyme in two crystal forms is reported. One of the these crystal forms provides diffraction data to a resolution of 1.7 A...

Research Grants25

  1. GENETICS OF IRON ACQUISITION BY LEGIONELLA PNEUMOPHILA
    Nicholas Cianciotto; Fiscal Year: 2007
    ..abstract_text> ..
  2. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas Cianciotto; Fiscal Year: 2007
    ....
  3. GENETICS OF IRON ACQUISITION BY LEGIONELLA PNEUMOPHILA
    Nicholas Cianciotto; Fiscal Year: 2009
    ..abstract_text> ..
  4. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas Cianciotto; Fiscal Year: 2009
    ....
  5. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas P Cianciotto; Fiscal Year: 2010
    ....
  6. GENETICS OF IRON ACQUISITION BY LEGIONELLA PNEUMOPHILA
    Nicholas P Cianciotto; Fiscal Year: 2010
    ....
  7. Sixth International Conference on Legionella
    Nicholas Cianciotto; Fiscal Year: 2005
    ..This unique gathering of microbiologists, physicians, public health officials, and engineers is the definitive meeting for those concerned with Legionella and its associated diseases. ..
  8. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas Cianciotto; Fiscal Year: 2003
    ..The results of this investigation will not only enhance our understanding of legionellosis but will serve as a model for the study of other intracellular pathogens. ..
  9. GENETICS OF IRON ACQUISITION BY LEGIONELLA PNEUMOPHILA
    Nicholas Cianciotto; Fiscal Year: 2003
    ..Taken together, the proposed experiments will offer greater insight into Legionella pathogenesis as well as intracellular parasitism and microbial iron acquisition in general. ..
  10. TYPE II SECRETION AND LEGIONELLA PNEUMOPHILA INFECTION
    Nicholas P Cianciotto; Fiscal Year: 2011
    ....