Richard Carthew

Summary

Affiliation: Northwestern University
Country: USA

Publications

  1. ncbi request reprint Gene regulation by microRNAs
    Richard W Carthew
    Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Drive, Northwestern University, Evanston, IL 60208, USA
    Curr Opin Genet Dev 16:203-8. 2006
  2. pmc Origins and Mechanisms of miRNAs and siRNAs
    Richard W Carthew
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
    Cell 136:642-55. 2009
  3. ncbi request reprint RNA interference: the fragile X syndrome connection
    Richard W Carthew
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Curr Biol 12:R852-4. 2002
  4. pmc Pattern formation in the Drosophila eye
    Richard W Carthew
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, United States
    Curr Opin Genet Dev 17:309-13. 2007
  5. ncbi request reprint Molecular biology. A new RNA dimension to genome control
    Richard W Carthew
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Science 313:305-6. 2006
  6. ncbi request reprint Adhesion proteins and the control of cell shape
    Richard W Carthew
    Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Drive, Northwestern University, Evanston, IL 60208, USA
    Curr Opin Genet Dev 15:358-63. 2005
  7. ncbi request reprint Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways
    Young Sik Lee
    Department of Biochemistry, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Cell 117:69-81. 2004
  8. pmc RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E
    Jason R Kennerdell
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Genes Dev 16:1884-9. 2002
  9. pmc Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila
    Joao Trindade Marques
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois, USA
    Nat Struct Mol Biol 17:24-30. 2010
  10. ncbi request reprint A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila
    John W Pham
    Department of Biochemistry, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Cell 117:83-94. 2004

Research Grants

  1. Molecular Mechanisms of Gene Silencing by RNAi
    Richard Carthew; Fiscal Year: 2006
  2. MicroRNA regulation of biological mechanisms
    Richard Carthew; Fiscal Year: 2007
  3. Molecular Mechanisms of Gene Silencing by RNAi
    Richard Carthew; Fiscal Year: 2007
  4. Molecular Mechanisms of Gene Silencing by RNAi
    Richard Carthew; Fiscal Year: 2007
  5. MicroRNA regulation of biological mechanisms
    Richard Carthew; Fiscal Year: 2009
  6. Molecular Mechanisms of Gene Silencing by RNAi
    Richard W Carthew; Fiscal Year: 2010
  7. PHOTORECEPTOR CELL DEVELOPMENT AND SIGNAL TRANSDUCTION
    Richard Carthew; Fiscal Year: 2005
  8. PHOTORECEPTOR CELL DEVELOPMENT AND SIGNAL TRANSDUCTION
    Richard Carthew; Fiscal Year: 1993
  9. PHOTORECEPTOR CELL DEVELOPMENT AND SIGNAL TRANSDUCTION
    Richard Carthew; Fiscal Year: 2001
  10. MicroRNA regulation of biological mechanisms
    Richard W Carthew; Fiscal Year: 2010

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Gene regulation by microRNAs
    Richard W Carthew
    Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Drive, Northwestern University, Evanston, IL 60208, USA
    Curr Opin Genet Dev 16:203-8. 2006
    ..This mechanism is an efficient means to regulate production of a diverse range of proteins...
  2. pmc Origins and Mechanisms of miRNAs and siRNAs
    Richard W Carthew
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208 3500, USA
    Cell 136:642-55. 2009
    ..Our understanding of siRNA- and miRNA-based regulation has direct implications for fundamental biology as well as disease etiology and treatment...
  3. ncbi request reprint RNA interference: the fragile X syndrome connection
    Richard W Carthew
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Curr Biol 12:R852-4. 2002
    ..Now FMRP has been found to associate with the RNP complex that mediates post-transcriptional silencing by RNAi...
  4. pmc Pattern formation in the Drosophila eye
    Richard W Carthew
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, United States
    Curr Opin Genet Dev 17:309-13. 2007
    ..This sorting and culling process results in a sculpted pattern with a precise number and position of cells that is repeated hundreds of times in each compound eye...
  5. ncbi request reprint Molecular biology. A new RNA dimension to genome control
    Richard W Carthew
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Science 313:305-6. 2006
  6. ncbi request reprint Adhesion proteins and the control of cell shape
    Richard W Carthew
    Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Drive, Northwestern University, Evanston, IL 60208, USA
    Curr Opin Genet Dev 15:358-63. 2005
    ..Recent insights into adherens junction remodeling have revealed the importance of polarized localization of myosin and Par3 at the adherens junction...
  7. ncbi request reprint Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways
    Young Sik Lee
    Department of Biochemistry, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Cell 117:69-81. 2004
    ..However, Dicer-1 but not Dicer-2 is essential for miRISC-directed translation repression. Thus, siRISCs and miRISCs are different with respect to Dicers in Drosophila...
  8. pmc RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E
    Jason R Kennerdell
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Genes Dev 16:1884-9. 2002
    ....
  9. pmc Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila
    Joao Trindade Marques
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois, USA
    Nat Struct Mol Biol 17:24-30. 2010
    ..Loqs is primarily required for dsRNA processing, whereas R2D2 is essential for the subsequent loading of siRNAs into effector Ago-RISC complexes...
  10. ncbi request reprint A Dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila
    John W Pham
    Department of Biochemistry, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Cell 117:83-94. 2004
    ..Dcr-2 does not simply transfer siRNAs to a distinct effector complex, but rather assembles into RISC along with the siRNAs, indicating that its role extends beyond the initiation phase of RNAi...
  11. pmc Silencing by small RNAs is linked to endosomal trafficking
    Young Sik Lee
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60201, USA
    Nat Cell Biol 11:1150-6. 2009
    ..We suggest that the recycling of RISCs is promoted by MVBs, resulting in RISCs more effectively engaging with small RNA effectors and possibly target RNAs. It may provide a means to enhance the dynamics of RNA silencing in the cytoplasm...
  12. ncbi request reprint A microRNA mediates EGF receptor signaling and promotes photoreceptor differentiation in the Drosophila eye
    Xin Li
    Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Drive, Northwestern University, Evanston, Illinois 60208, USA
    Cell 123:1267-77. 2005
    ..Expression is switched when EGFR signaling transiently triggers Yan degradation. This two-tiered mechanism explains how signal transduction activity can robustly generate a stable change in gene-expression patterns...
  13. pmc Physical modeling of cell geometric order in an epithelial tissue
    Sascha Hilgenfeldt
    Engineering Sciences and Applied Mathematics, Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 105:907-11. 2008
    ....
  14. pmc Conversion of pre-RISC to holo-RISC by Ago2 during assembly of RNAi complexes
    Kevin Kim
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    RNA 13:22-9. 2007
    ..We have also identified a new intermediate complex in the RISC assembly pathway, pre-RISC, in which Ago2 is stably bound to double-stranded siRNA...
  15. ncbi request reprint Par-1 kinase establishes cell polarity and functions in Notch signaling in the Drosophila embryo
    Jennifer Bayraktar
    Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Drive, Northwestern University, Evanston, IL 60208, USA
    J Cell Sci 119:711-21. 2006
    ..Epistasis analysis indicates that Par-1 functions upstream of Notch and is critical for proper localization of the Notch ligand Delta...
  16. pmc Targets of microRNA regulation in the Drosophila oocyte proteome
    Kenji Nakahara
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 102:12023-8. 2005
    ..The preponderance of genes that control global protein abundance suggests this process is under tight control by miRNAs at the onset of fertilization...
  17. ncbi request reprint Silence from within: endogenous siRNAs and miRNAs
    Erik J Sontheimer
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
    Cell 122:9-12. 2005
    ..Bioinformatic analyses indicate that target genes in vertebrate species may number in the thousands...
  18. ncbi request reprint Surface mechanics mediate pattern formation in the developing retina
    Takashi Hayashi
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Nature 431:647-52. 2004
    ..Thus, simple patterned expression of N-cadherin results in a complex spatial pattern of cells owing to cellular surface mechanics...
  19. ncbi request reprint Molecular biology. Argonaute journeys into the heart of RISC
    Erik J Sontheimer
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Science 305:1409-10. 2004
  20. pmc Phyllopod acts as an adaptor protein to link the sina ubiquitin ligase to the substrate protein tramtrack
    Songhui Li
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Mol Cell Biol 22:6854-65. 2002
    ..These results illustrate how Ras signaling can modulate an E3 ligase activity not by the phosphorylation of substrate proteins but by regulating the expression of specific E3 adaptors...
  21. pmc Cell-type-specific transcription of prospero is controlled by combinatorial signaling in the Drosophila eye
    Takashi Hayashi
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    Development 135:2787-96. 2008
    ..The activity level, therefore, is dictated by the proper combination of highly cooperative selector and pre-pattern factors present in the cell...
  22. pmc Lola regulates cell fate by antagonizing Notch induction in the Drosophila eye
    Limin Zheng
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Mech Dev 125:18-29. 2008
    ..The predicted consequence of this mechanism is to sharpen a cell's responsiveness to Notch signaling by creating a threshold...
  23. ncbi request reprint Expanding roles for miRNAs and siRNAs in cell regulation
    Kenji Nakahara
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA
    Curr Opin Cell Biol 16:127-33. 2004
    ..As new microRNAs and their mRNA targets rapidly emerge, it is becoming apparent that RNA-based regulation of mRNAs may rival ubiquitination as a mechanism to control protein levels...
  24. ncbi request reprint A call to arms: coevolution of animal viruses and host innate immune responses
    Joao T Marques
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Trends Genet 23:359-64. 2007
    ..We review how RNAi is used as an antiviral strategy and the mechanisms that viruses have evolved to suppress the RNAi response...
  25. pmc A microRNA imparts robustness against environmental fluctuation during development
    Xin Li
    Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Drive, Northwestern University, Evanston, Illinois 60208, USA
    Cell 137:273-82. 2009
    ..We suggest that some conserved microRNAs like miR-7 may enter into novel genetic relationships to buffer developmental programs against variation and impart robustness to diverse regulatory networks...
  26. ncbi request reprint Making a better RNAi vector for Drosophila: use of intron spacers
    Young Sik Lee
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2153 North Campus Drive, Evanston, Illinois 60208, USA
    Methods 30:322-9. 2003
    ..Moreover, the presence of the intron spacer greatly enhances the stability of inverted-repeat sequences in bacteria, facilitating the cloning procedure...
  27. pmc BMP signaling goes posttranscriptional in a microRNA sort of way
    Catherine A Reinke
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Dev Cell 15:174-5. 2008
    ..expands our understanding of miRNA biogenesis and maturation, elucidating a mechanism by which extracellular signaling directs cell differentiation via posttranscriptional regulation of miRNA expression...
  28. pmc Cell adhesion and cortex contractility determine cell patterning in the Drosophila retina
    Jos Käfer
    Laboratoire de Spectrometrie Physique, Unite Mixte de Recherche 5588, Université Joseph Fourier Grenoble I and Centre National de la Recherche Scientifique, 140 Avenue de la Physique, 38402 Saint Martin d Heres, France
    Proc Natl Acad Sci U S A 104:18549-54. 2007
    ..Furthermore, by changing only the corresponding parameters, this model can describe the mutants with different numbers of cells or changes in cadherin expression...
  29. pmc Functional analysis of dicer-2 missense mutations in the siRNA pathway of Drosophila
    Do Hwan Lim
    Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam Dong, Seongbuk gu, Seoul 136 713, South Korea
    Biochem Biophys Res Commun 371:525-30. 2008
    ..We also found that the carboxyl-terminal region of R2D2 is essential for Dicer-2 binding. These results provide further insight into the structure-function relationship of Dicer, which plays a critical role in the siRNA pathway...
  30. ncbi request reprint Chibby, a nuclear beta-catenin-associated antagonist of the Wnt/Wingless pathway
    Ken Ichi Takemaru
    Howard Hughes Medical Institute, Room K536C Health Sciences Building, Campus Box 357750, Department of Pharmacology, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Nature 422:905-9. 2003
    ..In addition, epistasis experiments indicate that chibby acts downstream of wingless and upstream of armadillo...
  31. doi request reprint The birth and death of microRNA genes in Drosophila
    Jian Lu
    Department of Ecology and Evolution, University of Chicago, 1101 East 57th Street, Chicago, Illinois 60637, USA
    Nat Genet 40:351-5. 2008
    ..The estimated net gain of long-lived miRNA genes, which is not strongly affected by either the depth or the breadth (number of tissues) of sequencing, is 0.3 genes per Myr in Drosophila...
  32. ncbi request reprint Making and breaking with nucleases and small RNAs
    Richard W Carthew
    Nat Struct Biol 10:776-7. 2003

Research Grants29

  1. Molecular Mechanisms of Gene Silencing by RNAi
    Richard Carthew; Fiscal Year: 2006
    ..Moreover, RNAi has been used experimentally to probe gene function and holds promise as a therapeutic model, making its basic understanding an important biomedical research goal. ..
  2. MicroRNA regulation of biological mechanisms
    Richard Carthew; Fiscal Year: 2007
    ..The combined effects of microRNAs may affect the expression of many human genes, and misregulation of microRNAs may well underlie complex disease phenomena such as cancer susceptibility and progression. ..
  3. Molecular Mechanisms of Gene Silencing by RNAi
    Richard Carthew; Fiscal Year: 2007
    ..Moreover, RNAi has been used experimentally to probe gene function and holds great promise as a therapeutic model, making its basic understanding an important biomedical research goal. ..
  4. Molecular Mechanisms of Gene Silencing by RNAi
    Richard Carthew; Fiscal Year: 2007
    ..Moreover, RNAi has been used experimentally to probe gene function and holds great promise as a therapeutic model, making its basic understanding an important biomedical research goal. ..
  5. MicroRNA regulation of biological mechanisms
    Richard Carthew; Fiscal Year: 2009
    ..The combined effects of microRNAs may affect the expression of many human genes, and misregulation of microRNAs may well underlie complex disease phenomena such as cancer susceptibility and progression. ..
  6. Molecular Mechanisms of Gene Silencing by RNAi
    Richard W Carthew; Fiscal Year: 2010
    ..Moreover, RNAi has been used experimentally to probe gene function and holds great promise as a therapeutic model, making its basic understanding an important biomedical research goal. ..
  7. PHOTORECEPTOR CELL DEVELOPMENT AND SIGNAL TRANSDUCTION
    Richard Carthew; Fiscal Year: 2005
    ..abstract_text> ..
  8. PHOTORECEPTOR CELL DEVELOPMENT AND SIGNAL TRANSDUCTION
    Richard Carthew; Fiscal Year: 1993
    ....
  9. PHOTORECEPTOR CELL DEVELOPMENT AND SIGNAL TRANSDUCTION
    Richard Carthew; Fiscal Year: 2001
    ....
  10. MicroRNA regulation of biological mechanisms
    Richard W Carthew; Fiscal Year: 2010
    ..Additionally, microRNAs play important functions during infection by numerous viruses. This project is to understand how microRNAs control gene expression and their natural functions within the body. ..