Leighcraft A Shakes

Summary

Affiliation: North Carolina Central University
Country: USA

Publications

  1. pmc Using BAC transgenesis in zebrafish to identify regulatory sequences of the amyloid precursor protein gene in humans
    Leighcraft A Shakes
    Julius L Chambers Biomedical Biotechnology Research Institute and Department of Chemistry, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
    BMC Genomics 13:451. 2012
  2. pmc Minimal cross-recombination between wild-type and loxP511 sites in vivo facilitates truncating both ends of large DNA inserts in pBACe3.6 and related vectors
    Leighcraft A Shakes
    Julius L Chambers Biomedical Biotechnology Research Institute Durham, NC 27707, USA
    Nucleic Acids Res 33:e118. 2005
  3. pmc Context dependent function of APPb enhancer identified using enhancer trap-containing BACs as transgenes in zebrafish
    Leighcraft A Shakes
    Julius L Chambers Biomedical Biotechnology Research Institute, Department of Chemistry, North Carolina Central University, Durham, NC 27707, USA
    Nucleic Acids Res 36:6237-48. 2008
  4. pmc Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons
    Leighcraft A Shakes
    Julius L, Chambers Biomedical Biotechnology Research Institute and Department of Chemistry, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
    BMC Genomics 12:351. 2011
  5. ncbi request reprint Selecting transpositions using phage P1 headful packaging: new markerless transposons for functionally mapping long-range regulatory sequences in bacterial artificial chromosomes and P1-derived artificial chromosomes
    Pradeep K Chatterjee
    Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
    Anal Biochem 335:305-15. 2004
  6. pmc Replacing the wild type loxP site in BACs from the public domain with lox66 using a lox66 transposon
    Pradeep K Chatterjee
    Department of Chemistry, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
    BMC Res Notes 3:38. 2010
  7. pmc Mutually exclusive recombination of wild-type and mutant loxP sites in vivo facilitates transposon-mediated deletions from both ends of genomic DNA in PACs
    Pradeep K Chatterjee
    Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
    Nucleic Acids Res 32:5668-76. 2004

Detail Information

Publications7

  1. pmc Using BAC transgenesis in zebrafish to identify regulatory sequences of the amyloid precursor protein gene in humans
    Leighcraft A Shakes
    Julius L Chambers Biomedical Biotechnology Research Institute and Department of Chemistry, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
    BMC Genomics 13:451. 2012
    ..Here we identify the putative transcription factor binding sites responsible for this distal cis-acting regulation, and use that information to identify a regulatory region of the human APP gene...
  2. pmc Minimal cross-recombination between wild-type and loxP511 sites in vivo facilitates truncating both ends of large DNA inserts in pBACe3.6 and related vectors
    Leighcraft A Shakes
    Julius L Chambers Biomedical Biotechnology Research Institute Durham, NC 27707, USA
    Nucleic Acids Res 33:e118. 2005
    ....
  3. pmc Context dependent function of APPb enhancer identified using enhancer trap-containing BACs as transgenes in zebrafish
    Leighcraft A Shakes
    Julius L Chambers Biomedical Biotechnology Research Institute, Department of Chemistry, North Carolina Central University, Durham, NC 27707, USA
    Nucleic Acids Res 36:6237-48. 2008
    ..The methodology should help functionally map multiple noncontiguous regulatory elements in BACs with or without gene-coding sequences...
  4. pmc Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons
    Leighcraft A Shakes
    Julius L, Chambers Biomedical Biotechnology Research Institute and Department of Chemistry, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
    BMC Genomics 12:351. 2011
    ..Here we describe experiments designed to determine if a simpler and more flexible system could modify BACs so that they would be suitable for transgenesis into zebrafish or mouse embryos using the Tol2 transposase...
  5. ncbi request reprint Selecting transpositions using phage P1 headful packaging: new markerless transposons for functionally mapping long-range regulatory sequences in bacterial artificial chromosomes and P1-derived artificial chromosomes
    Pradeep K Chatterjee
    Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
    Anal Biochem 335:305-15. 2004
    ..The procedure nevertheless offers a potential approach to map recombinogenic sequences in BACs and PACs...
  6. pmc Replacing the wild type loxP site in BACs from the public domain with lox66 using a lox66 transposon
    Pradeep K Chatterjee
    Department of Chemistry, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
    BMC Res Notes 3:38. 2010
    ..Gene targeting to a pre-determined site on the chromosome is likely to alleviate the problem...
  7. pmc Mutually exclusive recombination of wild-type and mutant loxP sites in vivo facilitates transposon-mediated deletions from both ends of genomic DNA in PACs
    Pradeep K Chatterjee
    Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 1801 Fayetteville Street, Durham, NC 27707, USA
    Nucleic Acids Res 32:5668-76. 2004
    ....