A C Howlett

Summary

Affiliation: North Carolina Central University
Country: USA

Publications

  1. ncbi request reprint The cannabinoid receptors
    Allyn C Howlett
    Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham 27707, USA
    Prostaglandins Other Lipid Mediat 68:619-31. 2002
  2. pmc Efficacy in CB1 receptor-mediated signal transduction
    Allyn C Howlett
    Neuroscience of Drug Abuse Research Program, Biomedical Biotechnology Research Institute, 700 George Street, North Carolina Central University, Durham, NC 27707, USA
    Br J Pharmacol 142:1209-18. 2004
  3. ncbi request reprint Cellular signal transduction by anandamide and 2-arachidonoylglycerol
    A C Howlett
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, 1402 South Grand Blvd, St Louis, MO 63104, USA
    Chem Phys Lipids 108:53-70. 2000
  4. ncbi request reprint Cannabinoid physiology and pharmacology: 30 years of progress
    Allyn C Howlett
    Neuroscience of Drug Abuse Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA
    Neuropharmacology 47:345-58. 2004
  5. ncbi request reprint Anandamide-mediated CB1/CB2 cannabinoid receptor--independent nitric oxide production in rabbit aortic endothelial cells
    LaTronya McCollum
    Neuroscience of Drug Abuse Research Program, J L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 700 George St, Durham, NC 27707, USA
    J Pharmacol Exp Ther 321:930-7. 2007
  6. ncbi request reprint Signal transduction of eicosanoid CB1 receptor ligands
    A C Howlett
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, MO 63104, USA
    Life Sci 65:617-25. 1999
  7. ncbi request reprint Azido- and isothiocyanato-substituted aryl pyrazoles bind covalently to the CB1 cannabinoid receptor and impair signal transduction
    A C Howlett
    Department of Pharmacological and Physiological Science, St Louis University, Missouri 63104, USA
    J Neurochem 74:2174-81. 2000
  8. ncbi request reprint Structural requirements for arachidonylethanolamide interaction with CB1 and CB2 cannabinoid receptors: pharmacology of the carbonyl and ethanolamide groups
    B A Berglund
    Department of Pharmacological and Physiological Science, St Louis University School of Medicine, MO 63104 1028, USA
    Prostaglandins Leukot Essent Fatty Acids 59:111-8. 1998
  9. ncbi request reprint Thujone exhibits low affinity for cannabinoid receptors but fails to evoke cannabimimetic responses
    J P Meschler
    Saint Louis University School of Medicine, Department of Pharmacological and Physiological Science, MO 63104, USA
    Pharmacol Biochem Behav 62:473-80. 1999
  10. ncbi request reprint Characterization of CB1 cannabinoid receptors using receptor peptide fragments and site-directed antibodies
    A C Howlett
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    Mol Pharmacol 53:504-10. 1998

Collaborators

Detail Information

Publications40

  1. ncbi request reprint The cannabinoid receptors
    Allyn C Howlett
    Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham 27707, USA
    Prostaglandins Other Lipid Mediat 68:619-31. 2002
    ..This paper will describe the biological activities, pharmacology, and signal transduction mechanisms for the cannabinoid receptors, with particular emphasis on the responses to the eicosanoid ligands...
  2. pmc Efficacy in CB1 receptor-mediated signal transduction
    Allyn C Howlett
    Neuroscience of Drug Abuse Research Program, Biomedical Biotechnology Research Institute, 700 George Street, North Carolina Central University, Durham, NC 27707, USA
    Br J Pharmacol 142:1209-18. 2004
    ..The possibility that CB(1) receptors interact with Gs or Gq will be evaluated, and questions with regard to the constitutive activity and G protein sequestration will be posed...
  3. ncbi request reprint Cellular signal transduction by anandamide and 2-arachidonoylglycerol
    A C Howlett
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, 1402 South Grand Blvd, St Louis, MO 63104, USA
    Chem Phys Lipids 108:53-70. 2000
    ..Cardiovascular regulation by anandamide appears to occur by a variety of receptor-mediated and non-receptor-mediated mechanisms. This review will describe and evaluate each of these signal transduction pathways and mechanisms...
  4. ncbi request reprint Cannabinoid physiology and pharmacology: 30 years of progress
    Allyn C Howlett
    Neuroscience of Drug Abuse Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA
    Neuropharmacology 47:345-58. 2004
    ..One very important role for CB1 antagonists will be in the treatment of craving in the disease of substance abuse...
  5. ncbi request reprint Anandamide-mediated CB1/CB2 cannabinoid receptor--independent nitric oxide production in rabbit aortic endothelial cells
    LaTronya McCollum
    Neuroscience of Drug Abuse Research Program, J L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 700 George St, Durham, NC 27707, USA
    J Pharmacol Exp Ther 321:930-7. 2007
    ....
  6. ncbi request reprint Signal transduction of eicosanoid CB1 receptor ligands
    A C Howlett
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, MO 63104, USA
    Life Sci 65:617-25. 1999
    ..Evidence suggests that the juxtamembrane region of the C-terminal of the CB1 receptor is critical for activation of these G-proteins...
  7. ncbi request reprint Azido- and isothiocyanato-substituted aryl pyrazoles bind covalently to the CB1 cannabinoid receptor and impair signal transduction
    A C Howlett
    Department of Pharmacological and Physiological Science, St Louis University, Missouri 63104, USA
    J Neurochem 74:2174-81. 2000
    ..These irreversibly binding antagonists might be useful tools for the investigation of tolerance and receptor down-regulation in both in vitro and in vivo studies...
  8. ncbi request reprint Structural requirements for arachidonylethanolamide interaction with CB1 and CB2 cannabinoid receptors: pharmacology of the carbonyl and ethanolamide groups
    B A Berglund
    Department of Pharmacological and Physiological Science, St Louis University School of Medicine, MO 63104 1028, USA
    Prostaglandins Leukot Essent Fatty Acids 59:111-8. 1998
    ..Stereochemical differences in the ethanolamide group influence the affinity for both cannabinoid receptor subtypes and the signal transduction capabilities of the methanandamide derivatives...
  9. ncbi request reprint Thujone exhibits low affinity for cannabinoid receptors but fails to evoke cannabimimetic responses
    J P Meschler
    Saint Louis University School of Medicine, Department of Pharmacological and Physiological Science, MO 63104, USA
    Pharmacol Biochem Behav 62:473-80. 1999
    ..Therefore, the hypothesis that activation of cannabinoid receptors is responsible for the intoxicating effects of thujone is not supported by the present data...
  10. ncbi request reprint Characterization of CB1 cannabinoid receptors using receptor peptide fragments and site-directed antibodies
    A C Howlett
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    Mol Pharmacol 53:504-10. 1998
    ....
  11. ncbi request reprint The CB(1) cannabinoid receptor juxtamembrane C-terminal peptide confers activation to specific G proteins in brain
    S Mukhopadhyay
    Department of Pharmacological Science, Saint Louis University School of Medicine, St Louis, Missouri, USA
    Mol Pharmacol 57:162-70. 2000
    ..Furthermore, this study provides the first description of domain specificity for interaction with a selective set of G proteins...
  12. pmc Cannabinoid regulation of nitric oxide synthase I (nNOS) in neuronal cells
    Skyla T Carney
    Neuroscience of Drug Abuse Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA
    J Neuroimmune Pharmacol 4:338-49. 2009
    ..The findings of these studies provide information related to cannabinoid-mediated NO signal transduction in neuronal cells, which has important implications in the ongoing elucidation of the endocannabinoid system in the nervous system...
  13. ncbi request reprint CB1 cannabinoid receptor: cellular regulation and distribution in N18TG2 neuroblastoma cells
    H H McIntosh
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, 1402 S Grand Blvd, St Louis, MO 63104, USA
    Brain Res Mol Brain Res 53:163-73. 1998
    ..Only low-affinity binding was seen in the chromatin fraction. An hypothesis has been developed to explain these results and form the basis for future studies...
  14. ncbi request reprint Inverse agonist properties of N-(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2, 4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide HCl (SR141716A) and 1-(2-chlorophenyl)-4-cyano-5-(4-methoxyphenyl)-1H-pyrazole-3-carboxyl ic acid phenylamide (CP-272871) for th
    J P Meschler
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, MO 63104, USA
    Biochem Pharmacol 60:1315-23. 2000
    ..These results demonstrated that the aryl pyrazoles SR141716A and CP-272871 behave as antagonists and as inverse agonists in G-protein-mediated signal transduction in preparations of endogenously expressed CB(1) receptors...
  15. ncbi request reprint Development of a novel class of monocyclic and bicyclic alkyl amides that exhibit CB1 and CB2 cannabinoid receptor affinity and receptor activation
    B A Berglund
    Department of Pharmacological and Physiological Science, Saint Louis University School Medicine, MO 63104, USA
    Drug Des Discov 16:281-94. 2000
    ..Further, we predicted a spatial conformation for these compounds in a molecular model based on the pharmacophoric and structural requirements for binding to the CB1 cannabinoid receptor...
  16. pmc Cannabinoid receptor-mediated translocation of NO-sensitive guanylyl cyclase and production of cyclic GMP in neuronal cells
    Jenelle D Jones
    Neuroscience of Drug Abuse Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA
    Neuropharmacology 54:23-30. 2008
    ..We conclude that CB(1) receptors stimulate cyclic GMP production and that intracellular translocation of GC from cytosol to the membranes is intrinsic to the mechanism and may be a tonically active or endocannabinoid-regulated process...
  17. ncbi request reprint Ca(2+) mobilization through dorsal root ganglion Ca(2+)-sensing receptor stably expressed in HEK293 cells
    Emmanuel M Awumey
    Cardiovascular Disease Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 700 George St, Durham, NC 27707, USA
    Am J Physiol Cell Physiol 292:C1895-905. 2007
    ..9 +/- 5.2% (at 1 mM Ca(2+)) and 40.5 +/- 6.5% (at 2 mM Ca(2+)), compared with controls. The findings suggest involvement of PKC in the pathway for Ca(2+) mobilization following CaR activation...
  18. ncbi request reprint Lipopolysaccharide and cyclic AMP regulation of CB(2) cannabinoid receptor levels in rat brain and mouse RAW 264.7 macrophages
    Somnath Mukhopadhyay
    Neuroscience of Drug Abuse Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA
    J Neuroimmunol 181:82-92. 2006
    ..Forskolin or dibutyryl cyclic AMP increased CB(2) receptor immunoreactivity, suggesting the involvement of the cyclic AMP-protein kinase A-Cyclic AMP response element pathway in the regulation of CB(2) receptor levels...
  19. ncbi request reprint WIN55212-2 docking to the CB1 cannabinoid receptor and multiple pathways for conformational induction
    Joong Youn Shim
    Neuroscience of Drug Abuse Research Program, J L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, North Carolina 27707, USA
    J Chem Inf Model 46:1286-300. 2006
    ..It is possible that differences in the nature of the ligand binding could contribute to ligand-specific conformational changes in the receptor...
  20. ncbi request reprint A short guide to the nomenclature of seven-transmembrane spanning receptors for lipid mediators
    Allyn C Howlett
    Neuroscience of Drug Abuse Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 700 George Street, Durham, NC 27707, USA
    Life Sci 77:1522-30. 2005
    ....
  21. ncbi request reprint Chemically distinct ligands promote differential CB1 cannabinoid receptor-Gi protein interactions
    Somnath Mukhopadhyay
    Neuroscience of Drug Abuse Research Program, J L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 700 George Street, Durham, NC 27707, USA
    Mol Pharmacol 67:2016-24. 2005
    ..These ligand-selective G protein responses imply that multiple conformations of the receptor could be evoked by ligands to regulate individual G proteins...
  22. ncbi request reprint Steric trigger as a mechanism for CB1 cannabinoid receptor activation
    Joong Youn Shim
    Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, North Carolina 27707, USA
    J Chem Inf Comput Sci 44:1466-76. 2004
    ..This mechanism would suggest that rotational flexibility in a ligand may be as important a determinant of agonist activity as the pharmacophoric elements that can be identified...
  23. pmc Drama-based education to motivate participation in substance abuse prevention
    Aileen B Stephens-Hernandez
    Neuroscience of Drug Abuse Research Program, Julius L Chambers Biomedical Biotechnology Research Institute EXPORT Center, North Carolina Central University, Durham, NC 27707, USA
    Subst Abuse Treat Prev Policy 2:11. 2007
    ..It was hypothesized that community-based education via drama would change attitudes toward alcohol and substance abuse, and increase participation in family and community activities aimed at substance abuse prevention...
  24. ncbi request reprint Endocannabinoids and reactive nitrogen and oxygen species in neuropathologies
    Allyn C Howlett
    Neuroscience of Drug Abuse Research Program, 208 Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 700 George Street, Durham, NC 27707, USA
    J Neuroimmune Pharmacol 1:305-16. 2006
    ..This review concentrates on cellular signal transduction pathways believed to be involved in the cellular damage...
  25. pmc Binding mode prediction of conformationally restricted anandamide analogs within the CB1 receptor
    Lea W Padgett
    Neuroscience of Drug Abuse Research Program, Julius L, Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA
    J Mol Signal 3:5. 2008
    ....
  26. ncbi request reprint Anandamide-induced vasorelaxation in rabbit aortic rings has two components: G protein dependent and independent
    Somnath Mukhopadhyay
    Department of Pharmacological and Physiological Science, St Louis University School of Medicine, St Louis, Missouri 63104, USA
    Am J Physiol Heart Circ Physiol 282:H2046-54. 2002
    ....
  27. ncbi request reprint Signal transduction interactions between CB1 cannabinoid and dopamine receptors in the rat and monkey striatum
    J P Meschler
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, 1402 South Grand Blvd, St Louis, MO 63104, USA
    Neuropharmacology 40:918-26. 2001
    ..Similar results obtained with both rat and monkey membranes indicate that striatal dopamine and cannabinoid interactions are conserved for these two species...
  28. ncbi request reprint International Union of Pharmacology. XXVII. Classification of cannabinoid receptors
    A C Howlett
    Neuroscience of Drug Abuse Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, North Carolina, USA
    Pharmacol Rev 54:161-202. 2002
    ..Although pharmacological evidence for the existence of additional types of cannabinoid receptor is emerging, other kinds of supporting evidence are still lacking...
  29. ncbi request reprint CB(1) cannabinoid receptor-G protein association: a possible mechanism for differential signaling
    Somnath Mukhopadhyay
    Neuroscience and Drug Abuse Research Program, J L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 700 George Street, Durham, NC 27707, USA
    Chem Phys Lipids 121:91-109. 2002
    ..In this review, we discuss several signal transduction pathways, focusing on studies that demonstrate the efficacy of CB(1) receptor agonists through G protein mediated pathways...
  30. ncbi request reprint CB1 receptor-G protein association. Subtype selectivity is determined by distinct intracellular domains
    S Mukhopadhyay
    Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, MO, USA
    Eur J Biochem 268:499-505. 2001
    ..These studies indicate that different domains of the CB(1) receptor direct the interaction with specific G protein subtypes...
  31. ncbi request reprint Nitric oxide regulates adenylyl cyclase activity in rat striatal membranes
    T Y Hudson
    Edward A. Doisy Department of Biochemistry and Molecular Biology, Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Neurochem 77:1279-84. 2001
    ..Under those conditions, complete inhibition was observed. The data indicate that NO attenuates neuromodulator-stimulated cAMP signaling in the striatum...
  32. ncbi request reprint Glucocorticoids plus opioids up-regulate genes that influence neuronal function
    Gregg R Ward
    Neuroscience of Drug Abuse Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC 27707, USA
    Cell Mol Neurobiol 27:651-60. 2007
    ..Future studies are warranted to determine if combined influences of glucocorticoid fluctuations and opioid receptor stimulation in vivo can orchestrate exagerated neuroadaptation to reinforcing drugs under chronic mild stress conditions...
  33. ncbi request reprint CB1 knockout mice display significant changes in striatal opioid peptide and D4 dopamine receptor gene expression
    Tonya M Gerald
    Neuroscience of Drug Abuse Research Program, Julius L Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, 700 George Street, Durham, NC 27707, USA
    Brain Res 1093:20-4. 2006
    ..These data suggest that genetic differences in CB(1) receptor may exert a modulatory effect on D(4) dopamine receptor and opioid peptide gene expression...
  34. ncbi request reprint Zinc inhibition of cAMP signaling
    Claudette Klein
    Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 277:11859-65. 2002
    ..The prestimulated enzyme was found to be less susceptible to Zn(2+) inhibition, suggesting that the ability of Zn(2+) to inhibit AC could be significantly influenced by the coincidence timing of the input signals to the enzyme...
  35. pmc Gene expression of opioid and dopamine systems in mouse striatum: effects of CB1 receptors, age and sex
    Tonya M Gerald
    Chemistry Department, North Carolina Central University, Durham, NC 27707, USA
    Psychopharmacology (Berl) 198:497-508. 2008
    ..Endocannabinoid, opioid, and dopamine systems interact to exhibit cannabinoid receptor neuromodulation of opioid peptides and D(4) dopamine receptor gene expression in CB(1)-cannabinoid-deficient mouse striatum...
  36. ncbi request reprint Helix 8 Leu in the CB1 cannabinoid receptor contributes to selective signal transduction mechanisms
    Sharon Anavi-Goffer
    California Pacific Medical Center Research Institute, San Francisco, California 94107, USA
    J Biol Chem 282:25100-13. 2007
    ..This study demonstrates that the evolutionary modification to NPXXY(X)(5,6)L contributes to maximal activity of the CB(1) receptor and provides a molecular basis for the differential coupling observed with chemically different agonists...
  37. ncbi request reprint Homology model of the CB1 cannabinoid receptor: sites critical for nonclassical cannabinoid agonist interaction
    Joong Youn Shim
    Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of South Carolina, Columbia, SC 29208, USA
    Biopolymers 71:169-89. 2003
    ..The present study provides a consistent framework for further investigation of the CB(1) receptor-ligand interaction and for the study of CB(1) receptor activation...
  38. ncbi request reprint Molecular interaction of the antagonist N-(piperidin-1-yl)-5-(4-chlorophenyl)-1- (2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide with the CB1 cannabinoid receptor
    Joong Youn Shim
    Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of South Carolina, Columbia, South Carolina 29208, USA
    J Med Chem 45:1447-59. 2002
    ..We further propose that the pyrazole C3 substituent of 1 might contribute to conferring either neutral antagonist or inverse agonist activity, depending upon the interaction with the receptor...
  39. ncbi request reprint Is there a role for anandamide in cardiovascular regulation? Insights from studies of endocannabinoid metabolism
    Emmanuel M Awumey
    Am J Physiol Heart Circ Physiol 289:H520-1. 2005
  40. pmc CB1 cannabinoid receptor activity is modulated by the cannabinoid receptor interacting protein CRIP 1a
    Jason L Niehaus
    Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, GA 30912, USA
    Mol Pharmacol 72:1557-66. 2007
    ..g., chronic pain, obesity, and epilepsy)...