Alexander Deiters

Summary

Affiliation: North Carolina State University
Country: USA

Publications

  1. ncbi request reprint MicroRNA miR-122 as a therapeutic target for oligonucleotides and small molecules
    M Thomas
    North Carolina State University, 2620 Yarbrough Drive, Raleigh, NC 27695, USA
    Curr Med Chem 20:3629-40. 2013
  2. pmc Light activation as a method of regulating and studying gene expression
    Alexander Deiters
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695 8204, USA
    Curr Opin Chem Biol 13:678-86. 2009
  3. ncbi request reprint Synthesis of oxygen- and nitrogen-containing heterocycles by ring-closing metathesis
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    Chem Rev 104:2199-238. 2004
  4. pmc Photocaged morpholino oligomers for the light-regulation of gene function in zebrafish and Xenopus embryos
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    J Am Chem Soc 132:15644-50. 2010
  5. pmc Small molecule modifiers of the microRNA and RNA interference pathway
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    AAPS J 12:51-60. 2010
  6. pmc Activation and deactivation of DNAzyme and antisense function with light for the photochemical regulation of gene expression in mammalian cells
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Am Chem Soc 132:6183-93. 2010
  7. pmc Spatiotemporal control of microRNA function using light-activated antagomirs
    Colleen M Connelly
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Mol Biosyst 8:2987-93. 2012
  8. pmc A photoactivatable small-molecule inhibitor for light-controlled spatiotemporal regulation of Rho kinase in live embryos
    Allison R Morckel
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    Development 139:437-42. 2012
  9. pmc The human mitochondrial tRNAMet: structure/function relationship of a unique modification in the decoding of unconventional codons
    Yann Bilbille
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695, USA
    J Mol Biol 406:257-74. 2011
  10. doi request reprint A light-activated DNA polymerase
    Chungjung Chou
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Angew Chem Int Ed Engl 48:5950-3. 2009

Collaborators

  • Jason W Chin
  • Estella M Gustilo
  • Jeffrey A Yoder
  • Paul F Agris
  • Michael S Bereman
  • M Thomas
  • Klaus M Hahn
  • Pradeep Kota
  • Nikolay V Dokholyan
  • Ning Wu
  • Shuo Chen
  • Marlene L Hauck
  • Daniel Summerer
  • Franck A P Vendeix
  • Douglas D Young
  • Hrvoje Lusic
  • Jeane M Govan
  • Colleen M Connelly
  • Mark O Lively
  • Yan Zou
  • James Hemphill
  • Andrew L McIver
  • Chungjung Chou
  • Qingyang Liu
  • Jesse A Teske
  • Rajendra Uprety
  • Laura Gardner
  • Lakshminath Sripada
  • Alex Prokup
  • Allison R Morckel
  • Yann Bilbille
  • Michael K Dush
  • Andrei V Karginov
  • Chad W Riggsbee
  • Shigeki J Miyake-Stoner
  • Wesleigh E Georgianna
  • Michael O Delaney
  • Wesleigh F Edwards
  • Kiranmai Gumireddy
  • Laila Farzana
  • Chad Riggsbee
  • Nanette M Nascone-Yoder
  • Meredith A Parr
  • Donna R Newman
  • Nanette Nascone-Yoder
  • Kimberly A Harris
  • Robert J Kaiser
  • T Ashton Cropp
  • Linda L Spremulli
  • David Shirvanyants
  • Julie Fisher
  • Alexandria Mara
  • Christie N Jones
  • Philip L Sannes
  • Christian A Refakis
  • Christoph Grohmann
  • Jared T Hammill
  • Jennifer L Hazen
  • Ryan A Mehl
  • Virginia A Moye
  • Jessica Torres-Kolbus
  • Qihong Huang
  • Rob Kaiser
  • Xin Xiong
  • William D Graham
  • Robert M Kelly
  • John B Hogenesch
  • William A Cantara
  • Jason Nichols
  • Alejandra Cruz-Montanez

Detail Information

Publications61

  1. ncbi request reprint MicroRNA miR-122 as a therapeutic target for oligonucleotides and small molecules
    M Thomas
    North Carolina State University, 2620 Yarbrough Drive, Raleigh, NC 27695, USA
    Curr Med Chem 20:3629-40. 2013
    ..Especially small molecule modifiers can display numerous advantages over nucleotide analogs, as discussed in this review. ..
  2. pmc Light activation as a method of regulating and studying gene expression
    Alexander Deiters
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695 8204, USA
    Curr Opin Chem Biol 13:678-86. 2009
    ..UV light irradiation then removes the caging group and activates the molecule, enabling control over gene activity with high spatial and temporal resolution...
  3. ncbi request reprint Synthesis of oxygen- and nitrogen-containing heterocycles by ring-closing metathesis
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    Chem Rev 104:2199-238. 2004
  4. pmc Photocaged morpholino oligomers for the light-regulation of gene function in zebrafish and Xenopus embryos
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    J Am Chem Soc 132:15644-50. 2010
    ..Our results suggest that photocaged morpholinos are readily synthesized and highly efficacious tools for light-activated spatiotemporal control of gene expression in multiple contexts...
  5. pmc Small molecule modifiers of the microRNA and RNA interference pathway
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    AAPS J 12:51-60. 2010
    ..Thus, the activation of tumor-suppressive miRNAs and the inhibition of oncogenic miRNAs by small molecules have the potential to provide a fundamentally new approach for the development of cancer therapeutics...
  6. pmc Activation and deactivation of DNAzyme and antisense function with light for the photochemical regulation of gene expression in mammalian cells
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Am Chem Soc 132:6183-93. 2010
    ..This represents the first example of gene silencing antisense agents that can be turned on and turned off in mammalian tissue culture...
  7. pmc Spatiotemporal control of microRNA function using light-activated antagomirs
    Colleen M Connelly
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Mol Biosyst 8:2987-93. 2012
    ..The presented approach enables the precise regulation of miRNA function and miRNA networks with unprecedented spatial and temporal resolution using UV irradiation and can be extended to any miRNA of interest...
  8. pmc A photoactivatable small-molecule inhibitor for light-controlled spatiotemporal regulation of Rho kinase in live embryos
    Allison R Morckel
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    Development 139:437-42. 2012
    ....
  9. pmc The human mitochondrial tRNAMet: structure/function relationship of a unique modification in the decoding of unconventional codons
    Yann Bilbille
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695, USA
    J Mol Biol 406:257-74. 2011
    ....
  10. doi request reprint A light-activated DNA polymerase
    Chungjung Chou
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Angew Chem Int Ed Engl 48:5950-3. 2009
  11. pmc Regulation of transcription through light-activation and light-deactivation of triplex-forming oligonucleotides in mammalian cells
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, 27695, United States
    ACS Chem Biol 7:1247-56. 2012
    ..In addition, hairpin loop structures were found to significantly increase the efficacy of phosphodiester DNA-based TFOs in tissue culture...
  12. doi request reprint Generating permissive site-specific unnatural aminoacyl-tRNA synthetases
    Shigeki J Miyake-Stoner
    Department of Chemistry, Franklin and Marshall College, Lancaster, Pennsylvania 17604 3003, USA
    Biochemistry 49:1667-77. 2010
    ..We expect that results of this work will increase the accessibility of UAA technology and the use of new UAAs in proteins...
  13. pmc Light-activated deoxyguanosine: photochemical regulation of peroxidase activity
    Hrvoje Lusic
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695, USA
    Mol Biosyst 4:508-11. 2008
    ..Photochemical activation of a deoxyribozyme with peroxidase activity was achieved by the synthesis and incorporation of a caged deoxyguanosine...
  14. pmc Stabilization and photochemical regulation of antisense agents through PEGylation
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    Bioconjug Chem 22:2136-42. 2011
    ..By using a photocleavable PEG group (PhotoPEG), antisense activity can be regulated with high spatial and temporal resolution, paving the way for precise regulation of gene expression in biological systems...
  15. pmc Photochemical regulation of restriction endonuclease activity
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Chembiochem 10:1612-6. 2009
    ..Interestingly, these groups do not appear to dramatically affect initial binding of the enzyme to the DNA substrate, but rather prevent recognition of the specific cleavage site...
  16. pmc Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, NC 27607 8204, USA
    Nucleic Acids Res 37:e58. 2009
    ..Additionally, this approach enables not only site-directed mutations, but also the insertion of DNA strands of any length into plasmids and the deletion of entire genes from plasmids...
  17. doi request reprint Microwave-assisted synthesis of unnatural amino acids
    Douglas D Young
    Department of Chemistry, North Carolina State University, Campus Box 8204, Raleigh, NC 27695 8204, USA
    Bioorg Med Chem Lett 18:5478-80. 2008
    ..Additional benefits of this methodology are the commercial availability of the inexpensive starting materials and the high yields and high purities of the final amino acid products...
  18. doi request reprint Light-regulated RNA-small molecule interactions
    Douglas D Young
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695 8204, USA
    Chembiochem 9:1225-8. 2008
  19. doi request reprint Phenanthridine synthesis via [2+2+2] cyclotrimerization reactions
    Lakshminath Sripada
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Org Biomol Chem 6:263-5. 2008
    ..A concise synthesis of phenanthridines via a microwave-assisted [2+2+2] cyclotrimerization reaction has been developed...
  20. doi request reprint Optochemical control of RNA interference in mammalian cells
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA, Department of Chemistry, College of William and Mary, Williamsburg, VA 32187, USA, Center for Structural Biology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA and Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Nucleic Acids Res 41:10518-28. 2013
    ..The ability to regulate both functions with light enables the application of this optochemical methodology to a wide range of small regulatory RNA molecules. ..
  21. pmc Cellular delivery and photochemical activation of antisense agents through a nucleobase caging strategy
    Jeane M Govan
    North Carolina State University, Department of Chemistry, Raleigh, North Carolina 27695, United States
    ACS Chem Biol 8:2272-82. 2013
    ..Importantly, the presented strategy delivers native oligonucleotides after light-activation, devoid of any delivery functionalities or modifications that could otherwise impair their antisense activity. ..
  22. doi request reprint Photochemical control of bacterial signal processing using a light-activated erythromycin
    Laura Gardner
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Mol Biosyst 7:2554-7. 2011
    ..coli. We applied this photochemical gene switch to the construction of a light-triggered logic gate, a light-controlled band-pass filter, as well as spatial and temporal control of gene expression...
  23. pmc High-throughput luciferase reporter assay for small-molecule inhibitors of microRNA function
    Colleen M Connelly
    North Carolina State University, Raleigh, NC, USA
    J Biomol Screen 17:822-8. 2012
    ..The described reporter assay will enable the high-throughput screening of small-molecule miR-122 inhibitors and can be readily extended to other miRNAs...
  24. doi request reprint Photocleavable polyethylene glycol for the light-regulation of protein function
    Wesleigh E Georgianna
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Bioconjug Chem 21:1404-7. 2010
    ..Herein, we report the synthesis of a photocleavable PEG reagent (PhotoPEG) and its application to the light-regulation of enzyme activity...
  25. pmc Heterotaxin: a TGF-β signaling inhibitor identified in a multi-phenotype profiling screen in Xenopus embryos
    Michael K Dush
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    Chem Biol 18:252-63. 2011
    ..Our results suggest that assessing multiple organ, tissue, cellular, and molecular parameters in a whole organism context is a valuable strategy for identifying the mechanism of action of bioactive compounds...
  26. ncbi request reprint Photochemical activation of protein expression in bacterial cells
    Douglas D Young
    North Carolina State University, Department of Chemistry, Campus Box 8204, Raleigh, NC 27695 8204, USA
    Angew Chem Int Ed Engl 46:4290-2. 2007
  27. doi request reprint Tricyclic alkaloid core structures assembled by a cyclotrimerization-coupled intramolecular nucleophilic substitution reaction
    Andrew L McIver
    North Carolina State University, Department of Chemistry, Raleigh, North Carolina 27695 8204, USA
    Org Lett 12:1288-91. 2010
    ..This methodology enabled the facile assembly of the spiroindolinone framework of citrinadins A and B, and cyclopiamine B...
  28. ncbi request reprint Photochemical control of biological processes
    Douglas D Young
    North Carolina State University, Department of Chemistry, Campus Box 8204, Raleigh, NC 27695, USA
    Org Biomol Chem 5:999-1005. 2007
    ..The majority of the described applications entail the utilization of photocaging groups installed either on a small molecule modulator of biomolecular function or directly on a biological macromolecule itself...
  29. pmc Photochemical control of DNA decoy function enables precise regulation of nuclear factor κB activity
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Am Chem Soc 133:13176-82. 2011
    ..This is the first example of a caged DNA decoy for the photochemical regulation of gene expression in mammalian cells and represents an important addition to the toolbox of light-controlled gene regulatory agents...
  30. pmc Photocaged t7 RNA polymerase for the light activation of transcription and gene function in pro- and eukaryotic cells
    Chungjung Chou
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Chembiochem 11:972-7. 2010
    ..The ability to achieve spatiotemporal regulation of orthogonal RNA synthesis enables the precise dissection and manipulation of a wide range of cellular events, including gene function...
  31. pmc Photochemical DNA activation
    Hrvoje Lusic
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Org Lett 9:1903-6. 2007
    ..The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function...
  32. doi request reprint Synthesis of anthracene and azaanthracene fluorophores via [2+2+2] cyclotrimerization reactions
    Yan Zou
    Department of Chemistry, North Carolina State University, Campus Box 8204, Raleigh, North Carolina 27695 8204, USA
    Org Lett 10:4661-4. 2008
    ..This is showcased in the assembly of a collection of fluorophores and their photochemical evaluation...
  33. doi request reprint Microwave activation of enzymatic catalysis
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Am Chem Soc 130:10048-9. 2008
    ..While these enzymes are inactive at low temperatures, they can be activated with microwave irradiation. To the best of our knowledge, this is the first illustration of a specific microwave effect in enzymatic catalysis...
  34. doi request reprint Genetically encoded light-activated transcription for spatiotemporal control of gene expression and gene silencing in mammalian cells
    James Hemphill
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    J Am Chem Soc 135:13433-9. 2013
    ..The synthetic gene expression system was validated with two reporter genes (luciferase and EGFP) and applied to the light-triggered transcription of short hairpin RNA constructs for the induction of RNA interference. ..
  35. pmc Improved synthesis of the two-photon caging group 3-nitro-2-ethyldibenzofuran and its application to a caged thymidine phosphoramidite
    Hrvoje Lusic
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    Org Lett 12:916-9. 2010
    ..Furthermore, its installation on a thymidine phosphoramidite is described. This caging group is efficiently removed through light-irradiation at 365 nm...
  36. ncbi request reprint Microwave-mediated nickel-catalyzed cyclotrimerization reactions: total synthesis of illudinine
    Jesse A Teske
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    J Org Chem 73:342-5. 2008
    ..This represents the first example of a Ni-catalyzed cyclotrimerization reaction in total synthesis...
  37. doi request reprint A cyclotrimerization route to cannabinoids
    Jesse A Teske
    North Carolina State University, Department of Chemistry, Raleigh, North Carolina 27695 8204, USA
    Org Lett 10:2195-8. 2008
    ..This approach provides a high level of synthetic flexibility allowing for the facile synthesis of cannabinoid analogues...
  38. ncbi request reprint Microwave-assisted solid-supported alkyne cyclotrimerization reactions for combinatorial chemistry
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    J Comb Chem 9:735-8. 2007
  39. doi request reprint Synthesis of the pyridine core of cyclothiazomycin
    Yan Zou
    North Carolina State University, Department of Chemistry, Raleigh, North Carolina 27695 8204, USA
    Org Lett 13:4352-5. 2011
    ....
  40. pmc Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet
    Hrvoje Lusic
    Department of Chemistry, Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695 and Dharmacon, 2650 Crescent Drive 100, Lafayette, CO 80026, USA
    Nucleic Acids Res 36:6548-57. 2008
    ..The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation...
  41. pmc A genetically encoded fluorescent amino acid
    Daniel Summerer
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, SR202, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 103:9785-9. 2006
    ..The strategy described here should be applicable to a number of different fluorophores in both prokaryotic and eukaryotic organisms, and it should facilitate both biochemical and cellular studies of protein structure and function...
  42. ncbi request reprint A genetically encoded photocaged tyrosine
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Angew Chem Int Ed Engl 45:2728-31. 2006
  43. pmc Recent advances in the photochemical control of protein function
    Chad W Riggsbee
    Department of Chemistry, North Carolina State University, Raleigh, NC 27607, USA
    Trends Biotechnol 28:468-75. 2010
    ..In addition, examples of the photochemical control of protein function through the application of genetically engineered natural-light receptors are presented...
  44. doi request reprint Total synthesis of cryptoacetalide
    Yan Zou
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Org Chem 75:5355-8. 2010
    ..Key steps of the synthesis are a microwave-mediated [2+2+2] cyclo-trimerization reaction to construct the central benzene ring, and a light-mediated radical cyclization to assemble the spiro-ketal moiety...
  45. pmc Light-controlled synthetic gene circuits
    Laura Gardner
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695, United States
    Curr Opin Chem Biol 16:292-9. 2012
    ..Herein we describe recent advancements in the area of light-controlled synthetic networks...
  46. doi request reprint DNA computation: a photochemically controlled AND gate
    Alex Prokup
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    J Am Chem Soc 134:3810-5. 2012
    ..This connection is important for the further development of an interface between DNA logic gates and electronic devices, enabling the connection of biological systems with electrical circuits...
  47. doi request reprint Small molecule modifiers of microRNA miR-122 function for the treatment of hepatitis C virus infection and hepatocellular carcinoma
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Am Chem Soc 132:7976-81. 2010
    ..In addition to providing a new approach for the development of therapeutics, small molecule modifiers of miR-122 function are unique tools for exploring miR-122 biogenesis...
  48. pmc Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP
    Andrei V Karginov
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States
    J Am Chem Soc 133:420-3. 2011
    ..Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo...
  49. doi request reprint Hydrogen peroxide induced activation of gene expression in mammalian cells using boronate estrone derivatives
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Angew Chem Int Ed Engl 51:9066-70. 2012
    ..This sensor is highly sensitive and specific for H(2)O(2)...
  50. doi request reprint The effect of microwave irradiation on DNA hybridization
    Wesleigh F Edwards
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Org Biomol Chem 7:2506-8. 2009
    ..These observations indicate a specific interaction of microwaves with DNA, and have important implications in the chemical or enzymatic processing of DNA under microwave heating...
  51. pmc Development of a robust and high throughput method for profiling N-linked glycans derived from plasma glycoproteins by NanoLC-FTICR mass spectrometry
    Michael S Bereman
    W M Keck FT ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Proteome Res 8:3764-70. 2009
    ..5% (RSD) when the internal standards were added prior to the initial processing step. The high mass measurement accuracy (<3 ppm) afforded by the FTICR mass spectrometer provided confident identifications of several glycan species...
  52. doi request reprint Identification of inhibitors of microRNA function from small molecule screens
    Colleen M Connelly
    Department of Chemistry, North Carolina State University, Raleigh, NC, USA
    Methods Mol Biol 1095:147-56. 2014
    ..Here, we report the application of a luciferase-based reporter assay of miRNA miR-122 function to the discovery of small molecule miR-122 inhibitors. ..
  53. doi request reprint Cellular microRNA sensors based on luciferase reporters
    Colleen M Connelly
    Department of Chemistry, North Carolina State University, Raleigh, NC, USA
    Methods Mol Biol 1095:135-46. 2014
    ..The steps described here can be applied not only to Huh7 cells and miR-122 but also to virtually any cell line and miRNA combination. ..
  54. doi request reprint Optochemical Control of Deoxyoligonucleotide Function via a Nucleobase-Caging Approach
    Qingyang Liu
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    Acc Chem Res 47:45-55. 2014
    ..More recently, we have developed light-activated DNA logic operations, an advance that may lay the foundation for the optochemical control of complex DNA calculations. ..
  55. doi request reprint DNA computation in mammalian cells: microRNA logic operations
    James Hemphill
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    J Am Chem Soc 135:10512-8. 2013
    ..Nucleic acid logic gates that are functional in a cellular environment and recognize endogenous inputs significantly expand the potential of DNA computation to monitor, image, and respond to cell-specific markers. ..
  56. ncbi request reprint Photochemical hammerhead ribozyme activation
    Douglas D Young
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695 8204, USA
    Bioorg Med Chem Lett 16:2658-61. 2006
    ..To achieve this effectively, we introduce an optimized N-caging group based on a nitrobenzyl core structure. This approach can potentially be employed toward a light-induced control of gene function...
  57. ncbi request reprint In vivo incorporation of an alkyne into proteins in Escherichia coli
    Alexander Deiters
    Department of Chemistry, Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Bioorg Med Chem Lett 15:1521-4. 2005
    ..These evolved tRNA-synthetase pairs were used to site-specifically incorporate an alkynyl group into a protein, which was subsequently conjugated with fluorescent dyes by a [3+2]-cycloaddition reaction under mild reaction conditions...
  58. ncbi request reprint Site-specific in vivo labeling of proteins for NMR studies
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Chembiochem 6:55-8. 2005
  59. ncbi request reprint General strategy for the syntheses of corynanthe, tacaman, and oxindole alkaloids
    Alexander Deiters
    Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712, USA
    J Org Chem 71:6547-61. 2006
    ..The ABD --> ABCD approach to these alkaloids featured a novel one-pot sequence of an RCM reaction and a zirconocene-catalyzed carbomagnesation followed by a second RCM to generate the D-ring...
  60. pmc Small-molecule inhibitors of microrna miR-21 function
    Kiranmai Gumireddy
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Angew Chem Int Ed Engl 47:7482-4. 2008
  61. ncbi request reprint Stereoselective total synthesis of dihydrocorynantheol
    Alexander Deiters
    Department of Chemistry and Biochemistry, The University of Texas, Austin 78712, USA
    Org Lett 4:3243-5. 2002
    ..Since no protecting groups were used, the present synthesis of 1 is exceedingly concise, consisting of only eight distinct operations...

Research Grants6

  1. Switchable Systems for Spatio-Temporal Control of Gene Expression in Zebrafish
    Alexander Deiters; Fiscal Year: 2007
    ..aptamers, expression plasmids, etc.) and protocols (e.g. for organic synthesis, UV irradiation, etc.) will be made accessible to the scientific community via a dedicated web-page. ..
  2. Switchable Systems for Spatio-Temporal Control of Gene Expression in Zebrafish
    Alexander Deiters; Fiscal Year: 2009
    ..aptamers, expression plasmids, etc.) and protocols (e.g. for organic synthesis, UV irradiation, etc.) will be made accessible to the scientific community via a dedicated web-page. ..
  3. Switchable Systems for Spatio-Temporal Control of Gene Expression in Zebrafish
    Alexander Deiters; Fiscal Year: 2010
    ..aptamers, expression plasmids, etc.) and protocols (e.g. for organic synthesis, UV irradiation, etc.) will be made accessible to the scientific community via a dedicated web-page. ..
  4. Switchable Systems for Spatio-Temporal Control of Gene Expression in Zebrafish
    Alexander Deiters; Fiscal Year: 2010
    ..aptamers, expression plasmids, etc.) and protocols (e.g. for organic synthesis, UV irradiation, etc.) will be made accessible to the scientific community via a dedicated web-page. ..