Alexander Deiters

Summary

Affiliation: North Carolina State University
Country: USA

Publications

  1. ncbi request reprint MicroRNA miR-122 as a therapeutic target for oligonucleotides and small molecules
    M Thomas
    North Carolina State University, 2620 Yarbrough Drive, Raleigh, NC 27695, USA
    Curr Med Chem 20:3629-40. 2013
  2. ncbi request reprint Synthesis of oxygen- and nitrogen-containing heterocycles by ring-closing metathesis
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    Chem Rev 104:2199-238. 2004
  3. pmc Photocaged morpholino oligomers for the light-regulation of gene function in zebrafish and Xenopus embryos
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    J Am Chem Soc 132:15644-50. 2010
  4. pmc Light activation as a method of regulating and studying gene expression
    Alexander Deiters
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695 8204, USA
    Curr Opin Chem Biol 13:678-86. 2009
  5. pmc Small molecule modifiers of the microRNA and RNA interference pathway
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    AAPS J 12:51-60. 2010
  6. pmc Optochemical control of RNA interference in mammalian cells
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA, Department of Chemistry, College of William and Mary, Williamsburg, VA 32187, USA, Center for Structural Biology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA and Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Nucleic Acids Res 41:10518-28. 2013
  7. pmc Activation and deactivation of DNAzyme and antisense function with light for the photochemical regulation of gene expression in mammalian cells
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Am Chem Soc 132:6183-93. 2010
  8. pmc Genetically encoded light-activated transcription for spatiotemporal control of gene expression and gene silencing in mammalian cells
    James Hemphill
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    J Am Chem Soc 135:13433-9. 2013
  9. pmc Spatiotemporal control of microRNA function using light-activated antagomirs
    Colleen M Connelly
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Mol Biosyst 8:2987-93. 2012
  10. pmc A photoactivatable small-molecule inhibitor for light-controlled spatiotemporal regulation of Rho kinase in live embryos
    Allison R Morckel
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    Development 139:437-42. 2012

Collaborators

  • Jason W Chin
  • Wenshe R Liu
  • Jeffrey A Yoder
  • Estella M Gustilo
  • Paul F Agris
  • Jie Zhang
  • Steven E Wheeler
  • Aravind Asokan
  • Gavin J Williams
  • Michael S Bereman
  • M Thomas
  • Nikolay V Dokholyan
  • Pradeep Kota
  • Klaus M Hahn
  • Shuo Chen
  • Ning Wu
  • Marlene L Hauck
  • Daniel Summerer
  • Franck A P Vendeix
  • Douglas D Young
  • James Hemphill
  • Hrvoje Lusic
  • Jeane M Govan
  • Colleen M Connelly
  • Rajendra Uprety
  • Yan Zou
  • Mark O Lively
  • Chungjung Chou
  • Andrew L McIver
  • Qingyang Liu
  • Jessica Torres-Kolbus
  • Laura Gardner
  • Jesse A Teske
  • Subhas Samanta
  • Yan Jiun Lee
  • Ji Luo
  • Yadagiri Kurra
  • Lakshminath Sripada
  • Kalyn A Brown
  • Michael Tsang
  • Jihe Liu
  • Steven Walsh
  • Yuta Naro
  • Yanyan Yang
  • David Shirvanyants
  • Allison R Morckel
  • Alex Prokup
  • Michael K Dush
  • Andrei V Karginov
  • Yann Bilbille
  • Chad W Riggsbee
  • Shigeki J Miyake-Stoner
  • Wesleigh E Georgianna
  • Michael O Delaney
  • Wesleigh F Edwards
  • Kiranmai Gumireddy
  • Rudolph L Juliano
  • Pavan K Mantravadi
  • Kalyn Brown
  • Erin K Borchardt
  • Tongxiang Lu
  • Duy P Nguyen
  • Jeane Govan
  • Keturah A Odoi
  • Laila Farzana
  • Chad Riggsbee
  • Nanette M Nascone-Yoder
  • Donna R Newman
  • Kimberly A Harris
  • Linda L Spremulli
  • Christie N Jones
  • Alexandria Mara
  • Meredith A Parr
  • Robert J Kaiser
  • Julie Fisher
  • T Ashton Cropp
  • Nanette Nascone-Yoder
  • Philip L Sannes
  • Jared T Hammill
  • Jennifer L Hazen
  • Christoph Grohmann
  • Christian A Refakis
  • Ryan A Mehl
  • Qihong Huang
  • Alejandra Cruz-Montanez
  • Robert M Kelly
  • William A Cantara
  • Rob Kaiser
  • William D Graham
  • Xin Xiong

Detail Information

Publications72

  1. ncbi request reprint MicroRNA miR-122 as a therapeutic target for oligonucleotides and small molecules
    M Thomas
    North Carolina State University, 2620 Yarbrough Drive, Raleigh, NC 27695, USA
    Curr Med Chem 20:3629-40. 2013
    ..Especially small molecule modifiers can display numerous advantages over nucleotide analogs, as discussed in this review. ..
  2. ncbi request reprint Synthesis of oxygen- and nitrogen-containing heterocycles by ring-closing metathesis
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    Chem Rev 104:2199-238. 2004
  3. pmc Photocaged morpholino oligomers for the light-regulation of gene function in zebrafish and Xenopus embryos
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    J Am Chem Soc 132:15644-50. 2010
    ..Our results suggest that photocaged morpholinos are readily synthesized and highly efficacious tools for light-activated spatiotemporal control of gene expression in multiple contexts...
  4. pmc Light activation as a method of regulating and studying gene expression
    Alexander Deiters
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695 8204, USA
    Curr Opin Chem Biol 13:678-86. 2009
    ..UV light irradiation then removes the caging group and activates the molecule, enabling control over gene activity with high spatial and temporal resolution...
  5. pmc Small molecule modifiers of the microRNA and RNA interference pathway
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    AAPS J 12:51-60. 2010
    ..Thus, the activation of tumor-suppressive miRNAs and the inhibition of oncogenic miRNAs by small molecules have the potential to provide a fundamentally new approach for the development of cancer therapeutics...
  6. pmc Optochemical control of RNA interference in mammalian cells
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA, Department of Chemistry, College of William and Mary, Williamsburg, VA 32187, USA, Center for Structural Biology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA and Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Nucleic Acids Res 41:10518-28. 2013
    ..The ability to regulate both functions with light enables the application of this optochemical methodology to a wide range of small regulatory RNA molecules. ..
  7. pmc Activation and deactivation of DNAzyme and antisense function with light for the photochemical regulation of gene expression in mammalian cells
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Am Chem Soc 132:6183-93. 2010
    ..This represents the first example of gene silencing antisense agents that can be turned on and turned off in mammalian tissue culture...
  8. pmc Genetically encoded light-activated transcription for spatiotemporal control of gene expression and gene silencing in mammalian cells
    James Hemphill
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    J Am Chem Soc 135:13433-9. 2013
    ..The synthetic gene expression system was validated with two reporter genes (luciferase and EGFP) and applied to the light-triggered transcription of short hairpin RNA constructs for the induction of RNA interference. ..
  9. pmc Spatiotemporal control of microRNA function using light-activated antagomirs
    Colleen M Connelly
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Mol Biosyst 8:2987-93. 2012
    ..The presented approach enables the precise regulation of miRNA function and miRNA networks with unprecedented spatial and temporal resolution using UV irradiation and can be extended to any miRNA of interest...
  10. pmc A photoactivatable small-molecule inhibitor for light-controlled spatiotemporal regulation of Rho kinase in live embryos
    Allison R Morckel
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    Development 139:437-42. 2012
    ....
  11. pmc The human mitochondrial tRNAMet: structure/function relationship of a unique modification in the decoding of unconventional codons
    Yann Bilbille
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695, USA
    J Mol Biol 406:257-74. 2011
    ....
  12. doi request reprint A light-activated DNA polymerase
    Chungjung Chou
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Angew Chem Int Ed Engl 48:5950-3. 2009
  13. pmc Regulation of transcription through light-activation and light-deactivation of triplex-forming oligonucleotides in mammalian cells
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, 27695, United States
    ACS Chem Biol 7:1247-56. 2012
    ..In addition, hairpin loop structures were found to significantly increase the efficacy of phosphodiester DNA-based TFOs in tissue culture...
  14. doi request reprint Generating permissive site-specific unnatural aminoacyl-tRNA synthetases
    Shigeki J Miyake-Stoner
    Department of Chemistry, Franklin and Marshall College, Lancaster, Pennsylvania 17604 3003, USA
    Biochemistry 49:1667-77. 2010
    ..We expect that results of this work will increase the accessibility of UAA technology and the use of new UAAs in proteins...
  15. pmc Light-activated deoxyguanosine: photochemical regulation of peroxidase activity
    Hrvoje Lusic
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695, USA
    Mol Biosyst 4:508-11. 2008
    ..Photochemical activation of a deoxyribozyme with peroxidase activity was achieved by the synthesis and incorporation of a caged deoxyguanosine...
  16. pmc Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, NC 27607 8204, USA
    Nucleic Acids Res 37:e58. 2009
    ..Additionally, this approach enables not only site-directed mutations, but also the insertion of DNA strands of any length into plasmids and the deletion of entire genes from plasmids...
  17. pmc Photochemical regulation of restriction endonuclease activity
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Chembiochem 10:1612-6. 2009
    ..Interestingly, these groups do not appear to dramatically affect initial binding of the enzyme to the DNA substrate, but rather prevent recognition of the specific cleavage site...
  18. pmc Stabilization and photochemical regulation of antisense agents through PEGylation
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    Bioconjug Chem 22:2136-42. 2011
    ..By using a photocleavable PEG group (PhotoPEG), antisense activity can be regulated with high spatial and temporal resolution, paving the way for precise regulation of gene expression in biological systems...
  19. pmc Light-regulated RNA-small molecule interactions
    Douglas D Young
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695 8204, USA
    Chembiochem 9:1225-8. 2008
  20. doi request reprint Phenanthridine synthesis via [2+2+2] cyclotrimerization reactions
    Lakshminath Sripada
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Org Biomol Chem 6:263-5. 2008
    ..A concise synthesis of phenanthridines via a microwave-assisted [2+2+2] cyclotrimerization reaction has been developed...
  21. doi request reprint Microwave-assisted synthesis of unnatural amino acids
    Douglas D Young
    Department of Chemistry, North Carolina State University, Campus Box 8204, Raleigh, NC 27695 8204, USA
    Bioorg Med Chem Lett 18:5478-80. 2008
    ..Additional benefits of this methodology are the commercial availability of the inexpensive starting materials and the high yields and high purities of the final amino acid products...
  22. pmc Genetically encoded optochemical probes for simultaneous fluorescence reporting and light activation of protein function with two-photon excitation
    Ji Luo
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States
    J Am Chem Soc 136:15551-8. 2014
    ....
  23. pmc Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes
    Yan Jiun Lee
    Department of Chemistry, Texas A and M University, College Station, TX 77843, USA
    Chem Commun (Camb) 50:13085-8. 2014
    ..These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells...
  24. doi request reprint Conditional Control of Alternative Splicing through Light-Triggered Splice-Switching Oligonucleotides
    James Hemphill
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States
    J Am Chem Soc 137:3656-62. 2015
    ..The use of light as a regulatory element allows for tight spatial and temporal control of splice switching in mammalian cells and animals. ..
  25. pmc Optochemical control of deoxyoligonucleotide function via a nucleobase-caging approach
    Qingyang Liu
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    Acc Chem Res 47:45-55. 2014
    ..More recently, we have developed light-activated DNA logic operations, an advance that may lay the foundation for the optochemical control of complex DNA calculations. ..
  26. pmc Cellular delivery and photochemical activation of antisense agents through a nucleobase caging strategy
    Jeane M Govan
    North Carolina State University, Department of Chemistry, Raleigh, North Carolina 27695, United States
    ACS Chem Biol 8:2272-82. 2013
    ..Importantly, the presented strategy delivers native oligonucleotides after light-activation, devoid of any delivery functionalities or modifications that could otherwise impair their antisense activity. ..
  27. doi request reprint Photochemical control of bacterial signal processing using a light-activated erythromycin
    Laura Gardner
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Mol Biosyst 7:2554-7. 2011
    ..coli. We applied this photochemical gene switch to the construction of a light-triggered logic gate, a light-controlled band-pass filter, as well as spatial and temporal control of gene expression...
  28. pmc High-throughput luciferase reporter assay for small-molecule inhibitors of microRNA function
    Colleen M Connelly
    North Carolina State University, Raleigh, NC, USA
    J Biomol Screen 17:822-8. 2012
    ..The described reporter assay will enable the high-throughput screening of small-molecule miR-122 inhibitors and can be readily extended to other miRNAs...
  29. ncbi request reprint Photochemical activation of protein expression in bacterial cells
    Douglas D Young
    North Carolina State University, Department of Chemistry, Campus Box 8204, Raleigh, NC 27695 8204, USA
    Angew Chem Int Ed Engl 46:4290-2. 2007
  30. pmc Heterotaxin: a TGF-β signaling inhibitor identified in a multi-phenotype profiling screen in Xenopus embryos
    Michael K Dush
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    Chem Biol 18:252-63. 2011
    ..Our results suggest that assessing multiple organ, tissue, cellular, and molecular parameters in a whole organism context is a valuable strategy for identifying the mechanism of action of bioactive compounds...
  31. doi request reprint Tricyclic alkaloid core structures assembled by a cyclotrimerization-coupled intramolecular nucleophilic substitution reaction
    Andrew L McIver
    North Carolina State University, Department of Chemistry, Raleigh, North Carolina 27695 8204, USA
    Org Lett 12:1288-91. 2010
    ..This methodology enabled the facile assembly of the spiroindolinone framework of citrinadins A and B, and cyclopiamine B...
  32. ncbi request reprint Photochemical control of biological processes
    Douglas D Young
    North Carolina State University, Department of Chemistry, Campus Box 8204, Raleigh, NC 27695, USA
    Org Biomol Chem 5:999-1005. 2007
    ..The majority of the described applications entail the utilization of photocaging groups installed either on a small molecule modulator of biomolecular function or directly on a biological macromolecule itself...
  33. doi request reprint Photocleavable polyethylene glycol for the light-regulation of protein function
    Wesleigh E Georgianna
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Bioconjug Chem 21:1404-7. 2010
    ..Herein, we report the synthesis of a photocleavable PEG reagent (PhotoPEG) and its application to the light-regulation of enzyme activity...
  34. pmc Photochemical DNA activation
    Hrvoje Lusic
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Org Lett 9:1903-6. 2007
    ..The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function...
  35. doi request reprint DNA computation in mammalian cells: microRNA logic operations
    James Hemphill
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    J Am Chem Soc 135:10512-8. 2013
    ..Nucleic acid logic gates that are functional in a cellular environment and recognize endogenous inputs significantly expand the potential of DNA computation to monitor, image, and respond to cell-specific markers. ..
  36. pmc Photochemical control of DNA decoy function enables precise regulation of nuclear factor κB activity
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Am Chem Soc 133:13176-82. 2011
    ..This is the first example of a caged DNA decoy for the photochemical regulation of gene expression in mammalian cells and represents an important addition to the toolbox of light-controlled gene regulatory agents...
  37. doi request reprint Synthesis of anthracene and azaanthracene fluorophores via [2+2+2] cyclotrimerization reactions
    Yan Zou
    Department of Chemistry, North Carolina State University, Campus Box 8204, Raleigh, North Carolina 27695 8204, USA
    Org Lett 10:4661-4. 2008
    ..This is showcased in the assembly of a collection of fluorophores and their photochemical evaluation...
  38. doi request reprint Microwave activation of enzymatic catalysis
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Am Chem Soc 130:10048-9. 2008
    ..While these enzymes are inactive at low temperatures, they can be activated with microwave irradiation. To the best of our knowledge, this is the first illustration of a specific microwave effect in enzymatic catalysis...
  39. pmc Photocaged t7 RNA polymerase for the light activation of transcription and gene function in pro- and eukaryotic cells
    Chungjung Chou
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA
    Chembiochem 11:972-7. 2010
    ..The ability to achieve spatiotemporal regulation of orthogonal RNA synthesis enables the precise dissection and manipulation of a wide range of cellular events, including gene function...
  40. pmc Synthesis of non-linear protein dimers through a genetically encoded Thiol-ene reaction
    Jessica Torres-Kolbus
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina, United States of America
    PLoS ONE 9:e105467. 2014
    ....
  41. doi request reprint Identification of inhibitors of microRNA function from small molecule screens
    Colleen M Connelly
    Department of Chemistry, North Carolina State University, Raleigh, NC, USA
    Methods Mol Biol 1095:147-56. 2014
    ..Here, we report the application of a luciferase-based reporter assay of miRNA miR-122 function to the discovery of small molecule miR-122 inhibitors. ..
  42. doi request reprint Cellular microRNA sensors based on luciferase reporters
    Colleen M Connelly
    Department of Chemistry, North Carolina State University, Raleigh, NC, USA
    Methods Mol Biol 1095:135-46. 2014
    ..The steps described here can be applied not only to Huh7 cells and miR-122 but also to virtually any cell line and miRNA combination. ..
  43. pmc Improved synthesis of the two-photon caging group 3-nitro-2-ethyldibenzofuran and its application to a caged thymidine phosphoramidite
    Hrvoje Lusic
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    Org Lett 12:916-9. 2010
    ..Furthermore, its installation on a thymidine phosphoramidite is described. This caging group is efficiently removed through light-irradiation at 365 nm...
  44. ncbi request reprint Microwave-mediated nickel-catalyzed cyclotrimerization reactions: total synthesis of illudinine
    Jesse A Teske
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    J Org Chem 73:342-5. 2008
    ..This represents the first example of a Ni-catalyzed cyclotrimerization reaction in total synthesis...
  45. ncbi request reprint Microwave-assisted solid-supported alkyne cyclotrimerization reactions for combinatorial chemistry
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    J Comb Chem 9:735-8. 2007
  46. doi request reprint A cyclotrimerization route to cannabinoids
    Jesse A Teske
    North Carolina State University, Department of Chemistry, Raleigh, North Carolina 27695 8204, USA
    Org Lett 10:2195-8. 2008
    ..This approach provides a high level of synthetic flexibility allowing for the facile synthesis of cannabinoid analogues...
  47. pmc A genetically encoded fluorescent amino acid
    Daniel Summerer
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, SR202, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 103:9785-9. 2006
    ..The strategy described here should be applicable to a number of different fluorophores in both prokaryotic and eukaryotic organisms, and it should facilitate both biochemical and cellular studies of protein structure and function...
  48. pmc Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet
    Hrvoje Lusic
    Department of Chemistry, Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695 and Dharmacon, 2650 Crescent Drive 100, Lafayette, CO 80026, USA
    Nucleic Acids Res 36:6548-57. 2008
    ..The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation...
  49. pmc Recent advances in the photochemical control of protein function
    Chad W Riggsbee
    Department of Chemistry, North Carolina State University, Raleigh, NC 27607, USA
    Trends Biotechnol 28:468-75. 2010
    ..In addition, examples of the photochemical control of protein function through the application of genetically engineered natural-light receptors are presented...
  50. doi request reprint Synthesis of the pyridine core of cyclothiazomycin
    Yan Zou
    North Carolina State University, Department of Chemistry, Raleigh, North Carolina 27695 8204, USA
    Org Lett 13:4352-5. 2011
    ....
  51. doi request reprint Total synthesis of cryptoacetalide
    Yan Zou
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Org Chem 75:5355-8. 2010
    ..Key steps of the synthesis are a microwave-mediated [2+2+2] cyclo-trimerization reaction to construct the central benzene ring, and a light-mediated radical cyclization to assemble the spiro-ketal moiety...
  52. ncbi request reprint A genetically encoded photocaged tyrosine
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Angew Chem Int Ed Engl 45:2728-31. 2006
  53. doi request reprint Light-cleavable rapamycin dimer as an optical trigger for protein dimerization
    Kalyn A Brown
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Chem Commun (Camb) 51:5702-5. 2015
    ..We developed an optically activated rapamycin dimer that does not induce FKBP12-FRB dimerization until exposed to light, and applied it to control kinase, protease, and recombinase function. ..
  54. doi request reprint The effect of microwave irradiation on DNA hybridization
    Wesleigh F Edwards
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Org Biomol Chem 7:2506-8. 2009
    ..These observations indicate a specific interaction of microwaves with DNA, and have important implications in the chemical or enzymatic processing of DNA under microwave heating...
  55. pmc Site-specific promoter caging enables optochemical gene activation in cells and animals
    James Hemphill
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States
    J Am Chem Soc 136:7152-8. 2014
    ....
  56. ncbi request reprint Photochemical hammerhead ribozyme activation
    Douglas D Young
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695 8204, USA
    Bioorg Med Chem Lett 16:2658-61. 2006
    ..To achieve this effectively, we introduce an optimized N-caging group based on a nitrobenzyl core structure. This approach can potentially be employed toward a light-induced control of gene function...
  57. doi request reprint DNA computation: a photochemically controlled AND gate
    Alex Prokup
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 8204, USA
    J Am Chem Soc 134:3810-5. 2012
    ..This connection is important for the further development of an interface between DNA logic gates and electronic devices, enabling the connection of biological systems with electrical circuits...
  58. pmc Light-controlled synthetic gene circuits
    Laura Gardner
    North Carolina State University, Department of Chemistry, Raleigh, NC 27695, United States
    Curr Opin Chem Biol 16:292-9. 2012
    ..Herein we describe recent advancements in the area of light-controlled synthetic networks...
  59. doi request reprint Small molecule modifiers of microRNA miR-122 function for the treatment of hepatitis C virus infection and hepatocellular carcinoma
    Douglas D Young
    Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Am Chem Soc 132:7976-81. 2010
    ..In addition to providing a new approach for the development of therapeutics, small molecule modifiers of miR-122 function are unique tools for exploring miR-122 biogenesis...
  60. pmc Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP
    Andrei V Karginov
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States
    J Am Chem Soc 133:420-3. 2011
    ..Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo...
  61. doi request reprint Hydrogen peroxide induced activation of gene expression in mammalian cells using boronate estrone derivatives
    Jeane M Govan
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Angew Chem Int Ed Engl 51:9066-70. 2012
    ..This sensor is highly sensitive and specific for H(2)O(2)...
  62. pmc Development of a robust and high throughput method for profiling N-linked glycans derived from plasma glycoproteins by NanoLC-FTICR mass spectrometry
    Michael S Bereman
    W M Keck FT ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    J Proteome Res 8:3764-70. 2009
    ..5% (RSD) when the internal standards were added prior to the initial processing step. The high mass measurement accuracy (<3 ppm) afforded by the FTICR mass spectrometer provided confident identifications of several glycan species...
  63. pmc Two rapid catalyst-free click reactions for in vivo protein labeling of genetically encoded strained alkene/alkyne functionalities
    Yadagiri Kurra
    Department of Chemistry, Texas A and M University, College Station, Texas 77843, United States
    Bioconjug Chem 25:1730-8. 2014
    ..Proteins bearing these noncanonical amino acids were successively labeled with a fluorescein tetrazine dye and a diaryl nitrilimine both in vitro and in living cells...
  64. doi request reprint Genetic encoding of caged cysteine and caged homocysteine in bacterial and mammalian cells
    Rajendra Uprety
    Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, Raleigh, NC 27695 USA
    Chembiochem 15:1793-9. 2014
    ..These discoveries provide a new tool for the optochemical control of protein function in mammalian cells and expand the set of genetically encoded unnatural amino acids. ..
  65. doi request reprint Optical Control of CRISPR/Cas9 Gene Editing
    James Hemphill
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States
    J Am Chem Soc 137:5642-5. 2015
    ....
  66. pmc Intracellular light-activation of riboswitch activity
    Steven Walsh
    Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, Raleigh, NC 27695 8204 USA
    Chembiochem 15:1346-51. 2014
    ..The ability to activate riboswitches by using light enables the interrogation and manipulation of a wide range of biological processes with high precision, and will have broad utility in the regulation of artificial genetic circuits...
  67. doi request reprint Control of oncogenic miRNA function by light-activated miRNA antagomirs
    Colleen M Connelly
    Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, 45 Dabney Hall, Raleigh, NC, 27695 8204, USA
    Methods Mol Biol 1165:99-114. 2014
    ..The presented approach enables the precise regulation of miRNA function with unprecedented spatial and temporal resolution using UV irradiation and can be readily extended to any miRNA of interest. ..
  68. ncbi request reprint In vivo incorporation of an alkyne into proteins in Escherichia coli
    Alexander Deiters
    Department of Chemistry, Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Bioorg Med Chem Lett 15:1521-4. 2005
    ..These evolved tRNA-synthetase pairs were used to site-specifically incorporate an alkynyl group into a protein, which was subsequently conjugated with fluorescent dyes by a [3+2]-cycloaddition reaction under mild reaction conditions...
  69. ncbi request reprint Site-specific in vivo labeling of proteins for NMR studies
    Alexander Deiters
    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 8204, USA
    Chembiochem 6:55-8. 2005
  70. ncbi request reprint Stereoselective total synthesis of dihydrocorynantheol
    Alexander Deiters
    Department of Chemistry and Biochemistry, The University of Texas, Austin 78712, USA
    Org Lett 4:3243-5. 2002
    ..Since no protecting groups were used, the present synthesis of 1 is exceedingly concise, consisting of only eight distinct operations...
  71. pmc Small-molecule inhibitors of microrna miR-21 function
    Kiranmai Gumireddy
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Angew Chem Int Ed Engl 47:7482-4. 2008
  72. ncbi request reprint General strategy for the syntheses of corynanthe, tacaman, and oxindole alkaloids
    Alexander Deiters
    Department of Chemistry and Biochemistry, University of Texas, Austin, Texas 78712, USA
    J Org Chem 71:6547-61. 2006
    ..The ABD --> ABCD approach to these alkaloids featured a novel one-pot sequence of an RCM reaction and a zirconocene-catalyzed carbomagnesation followed by a second RCM to generate the D-ring...