Matthew Breen

Summary

Affiliation: North Carolina State University
Country: USA

Publications

  1. pmc A genome assembly-integrated dog 1 Mb BAC microarray: a cytogenetic resource for canine cancer studies and comparative genomic analysis
    R Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, Raleigh, NC 27606, USA
    Cytogenet Genome Res 122:110-21. 2008
  2. pmc Chromosome aberrations in canine multicentric lymphomas detected with comparative genomic hybridisation and a panel of single locus probes
    R Thomas
    Oncology Research Section, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK
    Br J Cancer 89:1530-7. 2003
  3. pmc Gene selection and cancer type classification of diffuse large-B-cell lymphoma using a bivariate mixture model for two-species data
    Yuhua Su
    Dr Su s Statistics, Department of Human Nutrition, Food, and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA
    Hum Genomics 7:2. 2013
  4. ncbi request reprint Evolutionarily conserved cytogenetic changes in hematological malignancies of dogs and humans--man and his best friend share more than companionship
    Matthew Breen
    Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC, 27606, USA
    Chromosome Res 16:145-54. 2008
  5. pmc Update on genomics in veterinary oncology
    Matthew Breen
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, and Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC 27606, USA
    Top Companion Anim Med 24:113-21. 2009
  6. pmc Canine cytogenetics--from band to basepair
    M Breen
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, and Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA
    Cytogenet Genome Res 120:50-60. 2008
  7. pmc An integrated 4249 marker FISH/RH map of the canine genome
    Matthew Breen
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    BMC Genomics 5:65. 2004
  8. pmc Influence of genetic background on tumor karyotypes: evidence for breed-associated cytogenetic aberrations in canine appendicular osteosarcoma
    Rachael Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    Chromosome Res 17:365-77. 2009
  9. pmc 'Putting our heads together': insights into genomic conservation between human and canine intracranial tumors
    Rachael Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    J Neurooncol 94:333-49. 2009
  10. doi request reprint Microarray-based cytogenetic profiling reveals recurrent and subtype-associated genomic copy number aberrations in feline sarcomas
    Rachael Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    Chromosome Res 17:987-1000. 2009

Research Grants

Detail Information

Publications42

  1. pmc A genome assembly-integrated dog 1 Mb BAC microarray: a cytogenetic resource for canine cancer studies and comparative genomic analysis
    R Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, Raleigh, NC 27606, USA
    Cytogenet Genome Res 122:110-21. 2008
    ..This cytogenetically validated, genome integrated BAC clone panel has enormous potential for aiding gene discovery through a comparative approach to molecular oncology...
  2. pmc Chromosome aberrations in canine multicentric lymphomas detected with comparative genomic hybridisation and a panel of single locus probes
    R Thomas
    Oncology Research Section, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UK
    Br J Cancer 89:1530-7. 2003
    ..We have also assembled a panel of 41 canine chromosome-specific BAC probes that may be used for accurate and efficient chromosome identification in future studies of this nature...
  3. pmc Gene selection and cancer type classification of diffuse large-B-cell lymphoma using a bivariate mixture model for two-species data
    Yuhua Su
    Dr Su s Statistics, Department of Human Nutrition, Food, and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA
    Hum Genomics 7:2. 2013
    ....
  4. ncbi request reprint Evolutionarily conserved cytogenetic changes in hematological malignancies of dogs and humans--man and his best friend share more than companionship
    Matthew Breen
    Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC, 27606, USA
    Chromosome Res 16:145-54. 2008
    ..These data suggest that humans and dogs share an ancestrally retained pathogenetic basis for cancer and that cytogenetic evaluation of canine tumors may provide greater insight into the biology of tumorigenesis...
  5. pmc Update on genomics in veterinary oncology
    Matthew Breen
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, and Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC 27606, USA
    Top Companion Anim Med 24:113-21. 2009
    ..Here, we will review some of the advancements of genomics as they relate to veterinary oncology...
  6. pmc Canine cytogenetics--from band to basepair
    M Breen
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, and Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA
    Cytogenet Genome Res 120:50-60. 2008
    ..The cytogenetics 'toolbox' for the dog is now loaded. This review aims to provide a summary of some of the recent advancements in canine cytogenetics...
  7. pmc An integrated 4249 marker FISH/RH map of the canine genome
    Matthew Breen
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    BMC Genomics 5:65. 2004
    ..Understanding the genetic basis of both phenotypic variation and disease susceptibility in the dog provides new ways in which to dissect the genetics of human health and biology...
  8. pmc Influence of genetic background on tumor karyotypes: evidence for breed-associated cytogenetic aberrations in canine appendicular osteosarcoma
    Rachael Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    Chromosome Res 17:365-77. 2009
    ..These data indicate for the first time that individual genetic backgrounds, as defined by breed in dogs, influence tumor karyotypes in a cancer with extensive genomic instability...
  9. pmc 'Putting our heads together': insights into genomic conservation between human and canine intracranial tumors
    Rachael Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    J Neurooncol 94:333-49. 2009
    ....
  10. doi request reprint Microarray-based cytogenetic profiling reveals recurrent and subtype-associated genomic copy number aberrations in feline sarcomas
    Rachael Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    Chromosome Res 17:987-1000. 2009
    ..Further characterization of these regions may ultimately permit molecular distinction between ISAS and non-ISAS, as a tool for predicting tumor behavior and prognosis, as well as refining means for therapeutic intervention...
  11. doi request reprint A genome-wide approach to comparative oncology: high-resolution oligonucleotide aCGH of canine and human osteosarcoma pinpoints shared microaberrations
    Andrea Y Angstadt
    Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC, USA
    Cancer Genet 205:572-87. 2012
    ..Such regions of instability may thus have functional significance in the etiology of OS, the most common primary bone tumor in both species...
  12. doi request reprint Characterization of canine osteosarcoma by array comparative genomic hybridization and RT-qPCR: signatures of genomic imbalance in canine osteosarcoma parallel the human counterpart
    Andrea Y Angstadt
    Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, USA
    Genes Chromosomes Cancer 50:859-74. 2011
    ....
  13. ncbi request reprint A cytogenetically characterized, genome-anchored 10-Mb BAC set and CGH array for the domestic dog
    Rachael Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    J Hered 98:474-84. 2007
    ..We outline the value of a combined aCGH/FISH approach, together with a well-annotated dog genome assembly, in canine and comparative cancer studies...
  14. doi request reprint Refining tumor-associated aneuploidy through 'genomic recoding' of recurrent DNA copy number aberrations in 150 canine non-Hodgkin lymphomas
    Rachael Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    Leuk Lymphoma 52:1321-35. 2011
    ..Our data suggest that gene discovery in NHL may be enhanced through comparative studies exploiting the less complex association between CNAs and tumor pathogenesis in canine patients...
  15. pmc Construction of a 2-Mb resolution BAC microarray for CGH analysis of canine tumors
    Rachael Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA
    Genome Res 15:1831-7. 2005
    ..Here we provide examples of the varied applications of this resource to tumor cytogenetics, in combination with other molecular cytogenetic techniques...
  16. ncbi request reprint Array-based comparative genomic hybridization-guided identification of reference genes for normalization of real-time quantitative polymerase chain reaction assay data for lymphomas, histiocytic sarcomas, and osteosarcomas of dogs
    Pei Chien Tsai
    Department of Molecular Biomedical Science, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
    Am J Vet Res 73:1335-43. 2012
    ..To identify suitable reference genes for normalization of real-time quantitative PCR (RT-qPCR) assay data for common tumors of dogs...
  17. doi request reprint Anchoring the dog to its relatives reveals new evolutionary breakpoints across 11 species of the Canidae and provides new clues for the role of B chromosomes
    Shannon E Duke Becker
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA
    Chromosome Res 19:685-708. 2011
    ..We propose that the complex karyotype rearrangements associated with speciation of the Canidae reflect unstable chromosome regions described by the fragile breakage model...
  18. doi request reprint Extensive conservation of genomic imbalances in canine transmissible venereal tumors (CTVT) detected by microarray-based CGH analysis
    Rachael Thomas
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC, 27606, USA
    Chromosome Res 17:927-34. 2009
    ..These data suggest that the CTVT genome demonstrates a vast degree of both structural and numerical reorganization that is maintained during transmission among the domestic dog population...
  19. pmc Comparison of regional gene expression differences in the brains of the domestic dog and human
    Erin Kennerly
    Department of Genetics, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, NC 27695, USA
    Hum Genomics 1:435-43. 2004
    ..This study lays the foundation for detailed analysis of the population structure of transcriptional variation as it relates to cognitive and neurological phenotypes in the domestic dog...
  20. pmc Molecular cytogenetic characterization of canine histiocytic sarcoma: A spontaneous model for human histiocytic cancer identifies deletion of tumor suppressor genes and highlights influence of genetic background on tumor behavior
    Benoit Hedan
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA
    BMC Cancer 11:201. 2011
    ..This process would identify evolutionarily conserved cytogenetic changes to highlight regions of particular importance to HS biology...
  21. pmc Growth requirements and chromosomal instability of induced pluripotent stem cells generated from adult canine fibroblasts
    Sehwon Koh
    Genomics Program, North Carolina State University, Raleigh, NC 27607, USA
    Stem Cells Dev 22:951-63. 2013
    ..The isolated iPSCs have similar characteristics to ESCs from other species, but the exact cellular mechanisms behind their unique co-dependency on both FGF2 and LIF are still unknown...
  22. ncbi request reprint Partial cytogenetic response with toceranib and prednisone treatment in a young dog with chronic monocytic leukemia
    Mayrim L Pérez
    Departments of aSmall Animal Clinical Sciences bPhysiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida cDepartment of Molecular Biomedical Sciences dDepartment of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh eCancer Genetics Program, UNC Lineberger Comprehensive Cancer Center, Chapel Hill, North Carolina, USA
    Anticancer Drugs 24:1098-103. 2013
    ..We report a case of CMoL in a young dog that achieved clinical remission with treatment with the tyrosine kinase inhibitor toceranib and prednisone...
  23. ncbi request reprint A novel canine lymphoma cell line: a translational and comparative model for lymphoma research
    William C Kisseberth
    Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA
    Leuk Res 31:1709-20. 2007
    ..This cell line and xenograft represent significant in vitro and in vivo models, respectively, for comparative and translational lymphoma research...
  24. doi request reprint Tackling the characterization of canine chromosomal breakpoints with an integrated in-situ/in-silico approach: the canine PAR and PAB
    Andrea C Young
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    Chromosome Res 16:1193-202. 2008
    ..The future application of the approach demonstrated in this study will prove useful when seeking to identify the genomic sequences surrounding recurrent chromosome breakpoints present in canine cancers...
  25. ncbi request reprint Molecular analysis of a spontaneous dystrophin 'knockout' dog
    S J Schatzberg
    College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA
    Neuromuscul Disord 9:289-95. 1999
    ..This new canine model should further our understanding of the physiological and biochemical processes in Duchenne muscular dystrophy...
  26. pmc FLT3 mutations in canine acute lymphocytic leukemia
    Steven E Suter
    Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA
    BMC Cancer 11:38. 2011
    ..A small percentage of canine acute lymphocytic leukemias (ALL) also exhibit FLT3 ITD mutations...
  27. doi request reprint BCR-ABL translocation in a dog with chronic monocytic leukemia
    Janice A Cruz Cardona
    Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA
    Vet Clin Pathol 40:40-7. 2011
    ..This may have clinical implications for treatment of dogs with chronic leukemias associated with particular genetic mutations. However, more case studies are needed to further characterize this disease...
  28. doi request reprint Molecular characterization of canine BCR-ABL-positive chronic myelomonocytic leukemia before and after chemotherapy
    Sarah Culver
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA
    Vet Clin Pathol 42:314-22. 2013
    ..Further understanding of the genetic aberrations involved in canine leukemia may be crucial to improve treatment protocols...
  29. ncbi request reprint Integrated cytogenetic BAC map of the genome of the gray, short-tailed opossum, Monodelphis domestica
    S E Duke
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 4700 Hillsborough Street, North Carolina State University, Raleigh, NC 27606, USA
    Chromosome Res 15:361-70. 2007
    ....
  30. pmc Enhancement of extra chromosomal recombination in somatic cells by affecting the ratio of homologous recombination (HR) to non-homologous end joining (NHEJ)
    Gretchen M Zaunbrecher
    Department of Veterinary Anatomy and Public Health, College of Veterinary Medicine, Texas A and M University, College Station, Texas, USA
    Anim Biotechnol 19:6-21. 2008
    ....
  31. ncbi request reprint Cerebellar cortical degeneration in adult American Staffordshire Terriers
    Natasha Olby
    Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    J Vet Intern Med 18:201-8. 2004
    ..Many spinocerebellar ataxias in humans are caused by nucleotide repeats, and this genetic aberration merits investigation as a potential cause of the disease in American Staffordshire Terriers...
  32. ncbi request reprint Microarray analysis of differentially expressed genes of primary tumors in the canine central nervous system
    S A M Thomson
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 4700 Hillsborough Street, Raleigh, NC 27606, USA
    Vet Pathol 42:550-8. 2005
    ..This report represents the first global gene expression analysis of different types of canine brain tumors by cDNA microarrays and might aid in the identification of potential candidate genes involved in tumor formation and progression...
  33. ncbi request reprint Genome sequence, comparative analysis and haplotype structure of the domestic dog
    Kerstin Lindblad-Toh
    Broad Institute of Harvard and MIT, 320 Charles Street, Cambridge, Massachusetts 02141, USA
    Nature 438:803-19. 2005
    ..The current SNP map now makes it possible for genome-wide association studies to identify genes responsible for diseases and traits, with important consequences for human and companion animal health...
  34. ncbi request reprint Genome of the marsupial Monodelphis domestica reveals innovation in non-coding sequences
    Tarjei S Mikkelsen
    Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 447:167-77. 2007
    ..A substantial proportion of these eutherian-specific CNEs arose from sequence inserted by transposable elements, pointing to transposons as a major creative force in the evolution of mammalian gene regulation...
  35. ncbi request reprint The search for a marsupial XIC reveals a break with vertebrate synteny
    Lance S Davidow
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Chromosome Res 15:137-46. 2007
    ..The latter argues that marsupial XCI does not require XIST and opens the search for alternative mechanisms of dosage compensation...
  36. ncbi request reprint The cnm locus, a canine homologue of human autosomal forms of centronuclear myopathy, maps to chromosome 2
    Laurent Tiret
    UMR 955 INRA ENVA de Génétique Moléculaire et Cellulaire, Ecole Nationale Veterinaire d Alfort, Maisons Alfort, France
    Hum Genet 113:297-306. 2003
    ..To date, no human MTM1 gene member has been mapped to this genetic region. This report thus describes the first spontaneous mammalian model of centronuclear myopathy and defines a new locus for this group of diseases...
  37. ncbi request reprint A mutation in the canine BHD gene is associated with hereditary multifocal renal cystadenocarcinoma and nodular dermatofibrosis in the German Shepherd dog
    Frode Lingaas
    Norwegian School of Veterinary Science, Oslo
    Hum Mol Genet 12:3043-53. 2003
    ..Strong evidence is provided that the RCND mutation may have a homozygous lethal effect (P<0.01)...
  38. ncbi request reprint Distinct B-cell and T-cell lymphoproliferative disease prevalence among dog breeds indicates heritable risk
    Jaime F Modiano
    Department of Immunology, AMC Cancer Center, Denver, Colorado 80214, USA
    Cancer Res 65:5654-61. 2005
    ..The data show that domestic dogs provide a unique and valuable resource to define factors that mediate risk as well as genes involved in the initiation of B-cell and T-cell LPD...
  39. ncbi request reprint A high-resolution comparative map of canine Chromosome 5q14.3-q33 constructed utilizing the 1.5x canine genome sequence
    Kenine E Comstock
    Clinical and Human Biology Divisions, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave, D4 100, P O Box 19024, Seattle, Washington 98109 1024, USA
    Mamm Genome 15:544-51. 2004
    ..Moreover, this study demonstrates the utility of genomic survey sequences when combined with accurate genome maps for rapid mapping of disease susceptibility loci...
  40. pmc Interleukin-12 inhibits tumor growth in a novel angiogenesis canine hemangiosarcoma xenograft model
    Nasim Akhtar
    Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, WI 53706, USA
    Neoplasia 6:106-16. 2004
    ..The endothelial cell model we have described offers unique opportunities to pursue further investigations with IL-12, as well as other antiangiogenic approaches in cancer therapy...
  41. ncbi request reprint The dog as a cancer model
    Chand Khanna
    Nat Biotechnol 24:1065-6. 2006
  42. ncbi request reprint Canine malignant hemangiosarcoma as a model of primitive angiogenic endothelium
    Susan P Fosmire
    AMC Cancer Research Center and Integrated Department of Immunology, University of Colorado Health Sciences Center, Denver, 80214, USA
    Lab Invest 84:562-72. 2004
    ..In summary, these cell lines represent a unique and novel resource to improve our understanding of endothelial cell biology in general and canine HSA in particular...

Research Grants5

  1. Canine Brain Tumors - A New Model for Gene Discovery
    Matthew Breen; Fiscal Year: 2006
    ..Characterization of these genetic loci will help to improve the definition of molecular risk and prognosis of human and canine brain tumors, with a potentially major impact on human health. ..
  2. SPONTANEOUS CANINE MODELS FOR CANCER GENE DISCOVERY
    Matthew Breen; Fiscal Year: 2007
    ..We propose that Man's best friend will provide the keys to unlocking some of nature's most intriguing puzzles about cancer. ..
  3. SPONTANEOUS CANINE MODELS FOR CANCER GENE DISCOVERY
    Matthew Breen; Fiscal Year: 2009
    ..We propose that Man's best friend will provide the keys to unlocking some of nature's most intriguing puzzles about cancer. ..
  4. SPONTANEOUS CANINE MODELS FOR CANCER GENE DISCOVERY
    Matthew Breen; Fiscal Year: 2010
    ..We propose that Man's best friend will provide the keys to unlocking some of nature's most intriguing puzzles about cancer. ..