Research Topics
| Aihua ZhangSummaryAffiliation: New England Biolabs Country: USA Publications
| Collaborators
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Detail Information
Publications
Fluorescent site-specific labeling of Escherichia coli expressed proteins with Sfp phosphopantetheinyl transferaseAihua Zhang
New England Biolabs, 01938 Ipswich, MA, USA
Methods Mol Biol 705:295-307. 2011..The fluorescently labeled E14.7 fusion protein was analyzed with a fluorescence imager and subsequently transfected into mammalian cells for imaging with a fluorescence microscope...
Site-specific protein labeling by intein-mediated protein ligationInca Ghosh
New England Biolabs, Ipswich, MA 01938, USA
Methods Mol Biol 705:87-107. 2011..This chapter also gives a general review of the critical parameters of intein-mediated cleavage and ligation reactions...
Development of SNAP-tag fluorogenic probes for wash-free fluorescence imagingXiaoli Sun
New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
Chembiochem 12:2217-26. 2011..This approach enables highly sensitive spatiotemporal investigation of protein dynamics in living cells...
Productive interaction of chaperones with substrate protein domains allows correct folding of the downstream GFP domainAihua Zhang
New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
Gene 350:25-31. 2005..A possible correlation between GFP fluorescence and the productive folding by chaperones is proposed. This study may provide a general strategy for identifying chaperones specific for difficult-to-fold proteins...
Study of protein splicing and intein-mediated peptide bond cleavage under high-cell-density conditionsShamik Sharma
New England Biolabs, 32 Tozer Road, Beverly, Massachusetts 01915, USA
Biotechnol Prog 19:1085-90. 2003..The results of this study demonstrated the potential feasibility of using the intein-mediated protein purification system for industrial-scale production of recombinant proteins...
