Ming Qun Xu

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. ncbi request reprint Recent advances in protein splicing: manipulating proteins in vitro and in vivo
    Ming Qun Xu
    New England Biolabs, Inc, 32 Tozer Road, Beverly, Massachusetts 01915, USA
    Curr Opin Biotechnol 16:440-6. 2005
  2. ncbi request reprint Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein
    Jie Xu
    New England Biolabs, Beverly, MA, USA
    Biotechniques 36:976-8, 980-1. 2004
  3. doi request reprint Fluorescent site-specific labeling of Escherichia coli expressed proteins with Sfp phosphopantetheinyl transferase
    Aihua Zhang
    New England Biolabs, 01938 Ipswich, MA, USA
    Methods Mol Biol 705:295-307. 2011
  4. ncbi request reprint Substrates for improved live-cell fluorescence labeling of SNAP-tag
    Ivan R Correa
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Curr Pharm Des 19:5414-20. 2013
  5. ncbi request reprint An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays
    Inca Ghosh
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    J Immunol Methods 293:85-95. 2004
  6. ncbi request reprint Purification of recombinant proteins from E. coli by engineered inteins
    Ming Qun Xu
    New England Biolabs, Inc, Beverly, MA, USA
    Methods Mol Biol 205:43-68. 2003
  7. pmc Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging
    Xiaoli Sun
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Chembiochem 12:2217-26. 2011
  8. ncbi request reprint Mechanistic and kinetic considerations of protein splicing
    Thomas C Evans TC JR
    New England Biolabs, Inc, 32 Tozer Road, Beverly, Massachusetts 01915, USA
    Chem Rev 102:4869-84. 2002
  9. ncbi request reprint Producing peptide arrays for epitope mapping by intein-mediated protein ligation
    Luo Sun
    New England Biolabs, Beverly, MA 01915, USA
    Biotechniques 37:430-6, 438, 440 passim. 2004
  10. pmc Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes
    Hang Gyeong Chin
    New England Biolabs, 32 Tozer Road, Beverly, MA 01915 5054, USA
    Proc Natl Acad Sci U S A 100:4510-5. 2003

Collaborators

  • Thomas C Evans
  • Inca Ghosh
  • Aihua Zhang
  • Kai Johnsson
  • Sriharsa Pradhan
  • Jie Xu
  • Luo Sun
  • Ivan R Correa
  • Sebastien Ferrandon
  • Brenda Baker
  • Xiaoli Sun
  • Ping Sun
  • Zhuo Yu Li
  • Zihe Rao
  • Hang Gyeong Chin
  • Yi Ding
  • Thomas C Evans TC JR
  • Luc Reymond
  • Grazvydas Lukinavicius
  • Christopher R Provost
  • Angela Howard
  • John Buswell
  • Damien Maurel
  • Christopher J Noren
  • Anastasiya Masharina
  • Sheng Ye
  • Ye Wei Shi
  • Chen Yun Guo
  • Jing Ming Yuan
  • Jeremy R Maunus
  • John Rush
  • Wolfgang E Trommer
  • Yan Jun Li
  • Fana Mersha
  • Torsten Sterzenbach
  • Lixin Chen
  • Gun Do Kim
  • Guillaume Lesage
  • Xuehui Chen
  • Ivan Marin
  • Fana B Mersha

Detail Information

Publications17

  1. ncbi request reprint Recent advances in protein splicing: manipulating proteins in vitro and in vivo
    Ming Qun Xu
    New England Biolabs, Inc, 32 Tozer Road, Beverly, Massachusetts 01915, USA
    Curr Opin Biotechnol 16:440-6. 2005
    ..Inteins have been utilized in the production of protein and peptide arrays, as molecular switches and in the reconstitution of functional proteins by split-gene techniques...
  2. ncbi request reprint Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein
    Jie Xu
    New England Biolabs, Beverly, MA, USA
    Biotechniques 36:976-8, 980-1. 2004
    ..We demonstrate the effectiveness of this approach by mutational analysis of peptide substrates derived from human cyclin-dependent kinase, Cdc2, which contains a phosphorylation site of human c-Src protein tyrosine kinase...
  3. doi request reprint Fluorescent site-specific labeling of Escherichia coli expressed proteins with Sfp phosphopantetheinyl transferase
    Aihua Zhang
    New England Biolabs, 01938 Ipswich, MA, USA
    Methods Mol Biol 705:295-307. 2011
    ..The fluorescently labeled E14.7 fusion protein was analyzed with a fluorescence imager and subsequently transfected into mammalian cells for imaging with a fluorescence microscope...
  4. ncbi request reprint Substrates for improved live-cell fluorescence labeling of SNAP-tag
    Ivan R Correa
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Curr Pharm Des 19:5414-20. 2013
    ..These new SNAP-tag substrates based on pure 6-regioisomers can significantly improve fluorescence labeling in live cells and should become powerful tools for bioimaging applications. ..
  5. ncbi request reprint An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays
    Inca Ghosh
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    J Immunol Methods 293:85-95. 2004
    ..These techniques do not require sophisticated equipment, reagents, or skills thereby providing a simple method for research and development...
  6. ncbi request reprint Purification of recombinant proteins from E. coli by engineered inteins
    Ming Qun Xu
    New England Biolabs, Inc, Beverly, MA, USA
    Methods Mol Biol 205:43-68. 2003
  7. pmc Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging
    Xiaoli Sun
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Chembiochem 12:2217-26. 2011
    ..This approach enables highly sensitive spatiotemporal investigation of protein dynamics in living cells...
  8. ncbi request reprint Mechanistic and kinetic considerations of protein splicing
    Thomas C Evans TC JR
    New England Biolabs, Inc, 32 Tozer Road, Beverly, Massachusetts 01915, USA
    Chem Rev 102:4869-84. 2002
  9. ncbi request reprint Producing peptide arrays for epitope mapping by intein-mediated protein ligation
    Luo Sun
    New England Biolabs, Beverly, MA 01915, USA
    Biotechniques 37:430-6, 438, 440 passim. 2004
    ..This novel approach allows for synthetic peptides to be uniformly arrayed onto membranes, compatible with a variety of applications...
  10. pmc Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes
    Hang Gyeong Chin
    New England Biolabs, 32 Tozer Road, Beverly, MA 01915 5054, USA
    Proc Natl Acad Sci U S A 100:4510-5. 2003
    ..Furthermore, these transgenic plants displayed improved resistance to the herbicide N-(phosphonomethyl)glycine (glyphosate) when compared with wild-type N. tabacum...
  11. ncbi request reprint Generation of an affinity column for antibody purification by intein-mediated protein ligation
    Luo Sun
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    J Immunol Methods 282:45-52. 2003
    ..Peptide epitopes possessing an N-terminal cysteine were ligated to the chitin bound CBD tag. We demonstrate that the resulting peptide columns permit the highly specific and efficient affinity purification of antibodies from animal sera...
  12. ncbi request reprint Protein splicing elements and plants: from transgene containment to protein purification
    Thomas C Evans
    New England Biolabs, Inc, Beverly, Massachusetts 01915, USA
    Annu Rev Plant Biol 56:375-92. 2005
    ..Finally, relevant techniques that may be used in plants in the future are discussed...
  13. ncbi request reprint A single surface tryptophan in the chitin-binding domain from Bacillus circulans chitinase A1 plays a pivotal role in binding chitin and can be modified to create an elutable affinity tag
    Sebastien Ferrandon
    New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Biochim Biophys Acta 1621:31-40. 2003
    ....
  14. doi request reprint Site-specific protein labeling by intein-mediated protein ligation
    Inca Ghosh
    New England Biolabs, Ipswich, MA 01938, USA
    Methods Mol Biol 705:87-107. 2011
    ..This chapter also gives a general review of the critical parameters of intein-mediated cleavage and ligation reactions...
  15. ncbi request reprint Crystal structures of an intein from the split dnaE gene of Synechocystis sp. PCC6803 reveal the catalytic model without the penultimate histidine and the mechanism of zinc ion inhibition of protein splicing
    Ping Sun
    Laboratory of Structural Biology, Tsinghua University, Beijing 100084, People s Republic of China
    J Mol Biol 353:1093-105. 2005
    ....
  16. ncbi request reprint Soluble expression and affinity purification of functional domain of human acetylcholine receptor alpha-subunit by the modulation of maltose binding protein
    Zhuo Yu Li
    Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, PR China
    Biotechnol Lett 26:1765-9. 2004
    ..Western blot analysis and ELISA showed that immunoreactivities of both MBP-AChR alpha205 and AChR alpha205 were similar to that of AChR alpha-subunit from Torpedo...
  17. ncbi request reprint Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing
    Yi Ding
    Laboratory of Structural Biology and the Ministry of Education Laboratory of Protein Science, School of Life Science and Engineering, Tsinghua University, Beijing 100084, People s Republic of China
    J Biol Chem 278:39133-42. 2003
    ..Our structural modeling suggests that this catalytic module is conserved in the C-terminal subdomains of inteins from diverse organisms...