Sanjay Tyagi

Summary

Affiliation: New York University
Country: USA

Publications

  1. ncbi Wavelength-shifting molecular beacons
    S Tyagi
    Department of Molecular Genetics, Public Health Research Institute, 455 First Avenue, New York, NY 10016, USA
    Nat Biotechnol 18:1191-6. 2000
  2. ncbi Multicolor molecular beacons for allele discrimination
    S Tyagi
    Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA
    Nat Biotechnol 16:49-53. 1998
  3. ncbi Molecular beacons: probes that fluoresce upon hybridization
    S Tyagi
    Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA
    Nat Biotechnol 14:303-8. 1996
  4. ncbi Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes
    Salvatore A E Marras
    Department of Molecular Genetics, Public Health Research Institute, Newark, NJ 07103, USA
    Clin Chim Acta 363:48-60. 2006
  5. pmc Mechanism of mRNA transport in the nucleus
    Diana Y Vargas
    Department of Molecular Genetics, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Proc Natl Acad Sci U S A 102:17008-13. 2005
  6. pmc Single-molecule imaging of transcriptionally coupled and uncoupled splicing
    Diana Y Vargas
    Public Health Research Institute, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    Cell 147:1054-65. 2011
  7. pmc tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells
    Musa M Mhlanga
    Department of Molecular Genetics, Public Health Research Institute 225 Warren Street, Newark, NJ 07103, USA
    Nucleic Acids Res 33:1902-12. 2005
  8. ncbi Using tRNA-linked molecular beacons to image cytoplasmic mRNAs in live cells
    Musa M Mhlanga
    Public Health Research Institute, 225 Warren Street, Newark, New Jersey 07103, USA
    Nat Protoc 1:1392-8. 2006
  9. pmc Real-time measurement of in vitro transcription
    Salvatore A E Marras
    Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Nucleic Acids Res 32:e72. 2004
  10. pmc Stochastic mRNA synthesis in mammalian cells
    Arjun Raj
    Courant Institute of Mathematical Sciences, New York University, New York, New York, USA
    PLoS Biol 4:e309. 2006

Collaborators

Detail Information

Publications21

  1. ncbi Wavelength-shifting molecular beacons
    S Tyagi
    Department of Molecular Genetics, Public Health Research Institute, 455 First Avenue, New York, NY 10016, USA
    Nat Biotechnol 18:1191-6. 2000
    ..We describe the spectral characteristics of wavelength-shifting molecular beacons, and we demonstrate how their use improves and simplifies multiplex genetic analyses...
  2. ncbi Multicolor molecular beacons for allele discrimination
    S Tyagi
    Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA
    Nat Biotechnol 16:49-53. 1998
    ..A comparison of "hairpin probes" with corresponding "linear probes" confirms that the presence of the hairpin stem in molecular beacons significantly enhances their specificity...
  3. ncbi Molecular beacons: probes that fluoresce upon hybridization
    S Tyagi
    Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA
    Nat Biotechnol 14:303-8. 1996
    ..When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced...
  4. ncbi Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes
    Salvatore A E Marras
    Department of Molecular Genetics, Public Health Research Institute, Newark, NJ 07103, USA
    Clin Chim Acta 363:48-60. 2006
    ..A number of formats for nucleic acid hybridization have been developed to identify DNA and RNA sequences that are involved in cellular processes and that aid in the diagnosis of genetic and infectious diseases...
  5. pmc Mechanism of mRNA transport in the nucleus
    Diana Y Vargas
    Department of Molecular Genetics, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Proc Natl Acad Sci U S A 102:17008-13. 2005
    ..This finding provides an explanation for a number of observations in which mRNA transport appeared to be an enzymatically facilitated process...
  6. pmc Single-molecule imaging of transcriptionally coupled and uncoupled splicing
    Diana Y Vargas
    Public Health Research Institute, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    Cell 147:1054-65. 2011
    ..This uncoupling occurs only on the perturbed introns, whereas the preceding and succeeding introns are removed cotranscriptionally. PAPERCLIP:..
  7. pmc tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells
    Musa M Mhlanga
    Department of Molecular Genetics, Public Health Research Institute 225 Warren Street, Newark, NJ 07103, USA
    Nucleic Acids Res 33:1902-12. 2005
    ..Our observations show that the fidelity of the tRNA export system is relaxed for unnatural tRNA variants when they are introduced into the nucleus in large amounts...
  8. ncbi Using tRNA-linked molecular beacons to image cytoplasmic mRNAs in live cells
    Musa M Mhlanga
    Public Health Research Institute, 225 Warren Street, Newark, New Jersey 07103, USA
    Nat Protoc 1:1392-8. 2006
    ..This protocol should take a total of 4 d to complete...
  9. pmc Real-time measurement of in vitro transcription
    Salvatore A E Marras
    Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Nucleic Acids Res 32:e72. 2004
    ..Utilizing these modified molecular beacons, quantitative comparisons were made of the activity of a variety of RNA polymerases and the effect of an inhibitor of transcription was determined...
  10. pmc Stochastic mRNA synthesis in mammalian cells
    Arjun Raj
    Courant Institute of Mathematical Sciences, New York University, New York, New York, USA
    PLoS Biol 4:e309. 2006
    ..These results demonstrate that gene expression in mammalian cells is subject to large, intrinsically random fluctuations and raise questions about how cells are able to function in the face of such noise...
  11. ncbi Genotyping SNPs with molecular beacons
    Salvatore A E Marras
    Department of Molecular Genetics, Public Health Research Institute, Newark, NJ, USA
    Methods Mol Biol 212:111-28. 2003
  12. doi Imaging intracellular RNA distribution and dynamics in living cells
    Sanjay Tyagi
    Public Health Research Institute, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA
    Nat Methods 6:331-8. 2009
    ..These methods have also been adopted to visualize and track single mRNA molecules in real time. This review explores the promises and limitations of these methods...
  13. pmc Use of sloppy molecular beacon probes for identification of mycobacterial species
    Hiyam H El-Hajj
    Public Health Research Institute, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    J Clin Microbiol 47:1190-8. 2009
    ..Utilizing this general paradigm, screening assays can be designed for the identification of a wide range of species...
  14. pmc Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes
    Salvatore A E Marras
    Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Nucleic Acids Res 30:e122. 2002
    ....
  15. pmc Imaging native beta-actin mRNA in motile fibroblasts
    Sanjay Tyagi
    Department of Molecular Genetics, Public Health Research Institute, Newark, New Jersey 07103, USA
    Biophys J 87:4153-62. 2004
    ..The use of cytoplasmically resident molecular beacons will enable further studies of the mechanism of beta-actin mRNA localization, and will be useful for understanding the dynamics of mRNA distribution in other living cells...
  16. doi Using molecular beacons to study dispersal of mRNPs from the gene locus
    Patrick T C van den Bogaard
    Public Health Research Institute, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA
    Methods Mol Biol 464:91-103. 2009
    ..These methods will be useful for studies of other dynamic processes such as mRNA export, splicing, and decay...
  17. pmc Visualizing the distribution and transport of mRNAs in living cells
    Diana P Bratu
    Department of Molecular Genetics, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
    Proc Natl Acad Sci U S A 100:13308-13. 2003
    ....
  18. pmc Imaging individual mRNA molecules using multiple singly labeled probes
    Arjun Raj
    Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Methods 5:877-9. 2008
    ..We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons...
  19. pmc Kaposi's Sarcoma-associated herpesvirus lytic switch protein stimulates DNA binding of RBP-Jk/CSL to activate the Notch pathway
    Kyla Driscoll Carroll
    Department of Microbiology and Molecular Genetics, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA
    J Virol 80:9697-709. 2006
    ..Accordingly, Rta, but not EBNA2 and NICD, reactivates the complete viral lytic cycle...
  20. ncbi Three amino acids in Escherichia coli CspE surface-exposed aromatic patch are critical for nucleic acid melting activity leading to transcription antitermination and cold acclimation of cells
    Sangita Phadtare
    Department of Biochemistry Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 277:46706-11. 2002
    ....
  21. pmc Expression of Th1-mediated immunity in mouse lungs induces a Mycobacterium tuberculosis transcription pattern characteristic of nonreplicating persistence
    Lanbo Shi
    Public Health Research Institute, Newark, NJ 07103, USA
    Proc Natl Acad Sci U S A 100:241-6. 2003
    ..The bacterial transcription changes measured at onset of Th1-mediated immunity are likely induced, directly or indirectly, by nitric oxide generated by infected macrophages...