Research Topics
Genomes and GenesSpecies | Sanjay TyagiSummaryAffiliation: New York University Country: USA Publications
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Detail Information
Publications
Wavelength-shifting molecular beaconsS Tyagi
Department of Molecular Genetics, Public Health Research Institute, 455 First Avenue, New York, NY 10016, USA
Nat Biotechnol 18:1191-6. 2000..We describe the spectral characteristics of wavelength-shifting molecular beacons, and we demonstrate how their use improves and simplifies multiplex genetic analyses...- Multicolor molecular beacons for allele discriminationS Tyagi
Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA
Nat Biotechnol 16:49-53. 1998..A comparison of "hairpin probes" with corresponding "linear probes" confirms that the presence of the hairpin stem in molecular beacons significantly enhances their specificity... - Molecular beacons: probes that fluoresce upon hybridizationS Tyagi
Department of Molecular Genetics, Public Health Research Institute, New York, NY 10016, USA
Nat Biotechnol 14:303-8. 1996..When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced... - Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probesSalvatore A E Marras
Department of Molecular Genetics, Public Health Research Institute, Newark, NJ 07103, USA
Clin Chim Acta 363:48-60. 2006..These assays can be followed in real time, providing quantitative determination of target nucleic acids over a broad range of concentrations... 
Mechanism of mRNA transport in the nucleusDiana Y Vargas
Department of Molecular Genetics, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
Proc Natl Acad Sci U S A 102:17008-13. 2005..This finding provides an explanation for a number of observations in which mRNA transport appeared to be an enzymatically facilitated process...- Single-molecule imaging of transcriptionally coupled and uncoupled splicingDiana Y Vargas
Public Health Research Institute, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
Cell 147:1054-65. 2011..This uncoupling occurs only on the perturbed introns, whereas the preceding and succeeding introns are removed cotranscriptionally. PAPERCLIP:.. - tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cellsMusa M Mhlanga
Department of Molecular Genetics, Public Health Research Institute 225 Warren Street, Newark, NJ 07103, USA
Nucleic Acids Res 33:1902-12. 2005..Our observations show that the fidelity of the tRNA export system is relaxed for unnatural tRNA variants when they are introduced into the nucleus in large amounts... - Using tRNA-linked molecular beacons to image cytoplasmic mRNAs in live cellsMusa M Mhlanga
Public Health Research Institute, 225 Warren Street, Newark, New Jersey 07103, USA
Nat Protoc 1:1392-8. 2006..This protocol should take a total of 4 d to complete... - Real-time measurement of in vitro transcriptionSalvatore A E Marras
Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
Nucleic Acids Res 32:e72. 2004..Utilizing these modified molecular beacons, quantitative comparisons were made of the activity of a variety of RNA polymerases and the effect of an inhibitor of transcription was determined... - Stochastic mRNA synthesis in mammalian cellsArjun Raj
Courant Institute of Mathematical Sciences, New York University, New York, New York, USA
PLoS Biol 4:e309. 2006..These results demonstrate that gene expression in mammalian cells is subject to large, intrinsically random fluctuations and raise questions about how cells are able to function in the face of such noise... - Genotyping SNPs with molecular beaconsSalvatore A E Marras
Department of Molecular Genetics, Public Health Research Institute, Newark, NJ, USA
Methods Mol Biol 212:111-28. 2003 
Imaging intracellular RNA distribution and dynamics in living cellsSanjay Tyagi
Public Health Research Institute, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA
Nat Methods 6:331-8. 2009..These methods have also been adopted to visualize and track single mRNA molecules in real time. This review explores the promises and limitations of these methods...- Use of sloppy molecular beacon probes for identification of mycobacterial speciesHiyam H El-Hajj
Public Health Research Institute, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
J Clin Microbiol 47:1190-8. 2009..Utilizing this general paradigm, screening assays can be designed for the identification of a wide range of species... - Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probesSalvatore A E Marras
Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
Nucleic Acids Res 30:e122. 2002.... - Imaging native beta-actin mRNA in motile fibroblastsSanjay Tyagi
Department of Molecular Genetics, Public Health Research Institute, Newark, New Jersey 07103, USA
Biophys J 87:4153-62. 2004..The use of cytoplasmically resident molecular beacons will enable further studies of the mechanism of beta-actin mRNA localization, and will be useful for understanding the dynamics of mRNA distribution in other living cells... - Using molecular beacons to study dispersal of mRNPs from the gene locusPatrick T C van den Bogaard
Public Health Research Institute, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA
Methods Mol Biol 464:91-103. 2009..These methods will be useful for studies of other dynamic processes such as mRNA export, splicing, and decay... - Visualizing the distribution and transport of mRNAs in living cellsDiana P Bratu
Department of Molecular Genetics, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA
Proc Natl Acad Sci U S A 100:13308-13. 2003.... - Imaging individual mRNA molecules using multiple singly labeled probesArjun Raj
Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Nat Methods 5:877-9. 2008..We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons... - Kaposi's Sarcoma-associated herpesvirus lytic switch protein stimulates DNA binding of RBP-Jk/CSL to activate the Notch pathwayKyla Driscoll Carroll
Department of Microbiology and Molecular Genetics, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA
J Virol 80:9697-709. 2006..Accordingly, Rta, but not EBNA2 and NICD, reactivates the complete viral lytic cycle... - Three amino acids in Escherichia coli CspE surface-exposed aromatic patch are critical for nucleic acid melting activity leading to transcription antitermination and cold acclimation of cellsSangita Phadtare
Department of Biochemistry Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
J Biol Chem 277:46706-11. 2002.... - Expression of Th1-mediated immunity in mouse lungs induces a Mycobacterium tuberculosis transcription pattern characteristic of nonreplicating persistenceLanbo Shi
Public Health Research Institute, Newark, NJ 07103, USA
Proc Natl Acad Sci U S A 100:241-6. 2003..The bacterial transcription changes measured at onset of Th1-mediated immunity are likely induced, directly or indirectly, by nitric oxide generated by infected macrophages...