Yuk Ching Tse-Dinh

Summary

Affiliation: New York Medical College
Country: USA

Publications

  1. ncbi request reprint Bacterial and archeal type I topoisomerases
    Y C Tse-Dinh
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Biochim Biophys Acta 1400:19-27. 1998
  2. pmc Bacterial topoisomerase I as a target for discovery of antibacterial compounds
    Yuk Ching Tse-Dinh
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Nucleic Acids Res 37:731-7. 2009
  3. ncbi request reprint An update on the development of drugs against smallpox
    Yuk Ching Tse-Dinh
    New York Medical College, Department of Biochemistry and Molecular Biology, 95 Grasslands Road, Valhalla, NY 10595, USA
    Curr Opin Investig Drugs 9:865-70. 2008
  4. ncbi request reprint Exploring DNA topoisomerases as targets of novel therapeutic agents in the treatment of infectious diseases
    Y C Tse-Dinh
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Infect Disord Drug Targets 7:3-9. 2007
  5. ncbi request reprint DNA supercoiling and bacterial adaptation: thermotolerance and thermoresistance
    Y C Tse-Dinh
    Dept of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595, USA
    Trends Microbiol 5:323-6. 1997
  6. pmc Increased sensitivity to oxidative challenges associated with topA deletion in Escherichia coli
    Y C Tse-Dinh
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Bacteriol 182:829-32. 2000
  7. pmc Bacterial cell killing mediated by topoisomerase I DNA cleavage activity
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 280:38489-95. 2005
  8. pmc The role of the Zn(II) binding domain in the mechanism of E. coli DNA topoisomerase I
    Adriana Ahumada
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY, USA
    BMC Biochem 3:13. 2002
  9. pmc Mutation adjacent to the active site tyrosine can enhance DNA cleavage and cell killing by the TOPRIM Gly to Ser mutant of bacterial topoisomerase I
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Nucleic Acids Res 36:1017-25. 2008
  10. ncbi request reprint Site-directed mutagenesis of residues involved in G Strand DNA binding by Escherichia coli DNA topoisomerase I
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 279:39207-13. 2004

Research Grants

  1. Development of a HTS system:topoisomerase targets (RMI)
    Yuk Ching Tse Dinh; Fiscal Year: 2004

Collaborators

  • Thirunavukkarasu Annamalai
  • I Fen Liu
  • Gagandeep Narula
  • Adriana Ahumada
  • Jon Clardy
  • Zhongtao Zhang
  • Tina K Van Dyk
  • Z Darzynkiewicz
  • Marc Drolet
  • X Li
  • Bokun Cheng
  • Sandra Aedo
  • Yuk Ching Tse Dinh
  • Jeanette H Sutherland
  • Elena P Sorokin
  • H Qi
  • Jingyang Feng
  • Shan Rui
  • Daniel Strahs
  • Natalee Stewart
  • Chang Xi Zhu
  • Sharvari Gadgil
  • Imad Baaklini
  • Chengling Ji
  • Priyanka Bansod
  • Shayna Sandhaus
  • Shugeng Cao
  • Victor Vásquez
  • Giselle Tamayo-Castillo
  • R R Kancherla
  • R Menzel
  • C W Lin
  • Maria Abrenica
  • Elena Sorokin
  • Sandra J Aedo
  • Siddarth Rathi
  • Maria V Abrenica
  • Jason Chen
  • Somshuvra Mukhopadhyay
  • Xiaoping Liu
  • Devyani Chaudhuri
  • Shikha Shukla
  • John W Foster
  • Sarinnapha Vasunilashorn
  • Fabien Rallu
  • Chadi Hraiky
  • Vishwaroop Mulay
  • Vivien W Gong
  • J S Nair
  • K Seiter
  • A Mannancheril
  • T Ahmed
  • H Durrani
  • E Bedner
  • F Traganos
  • C X Zhu

Detail Information

Publications41

  1. ncbi request reprint Bacterial and archeal type I topoisomerases
    Y C Tse-Dinh
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Biochim Biophys Acta 1400:19-27. 1998
    ....
  2. pmc Bacterial topoisomerase I as a target for discovery of antibacterial compounds
    Yuk Ching Tse-Dinh
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Nucleic Acids Res 37:731-7. 2009
    ..Topoisomerase I targeting poisons may be particularly effective when the bacterial pathogen is responding to host defense, or in the presence of other antibiotics that induce the bacterial stress response...
  3. ncbi request reprint An update on the development of drugs against smallpox
    Yuk Ching Tse-Dinh
    New York Medical College, Department of Biochemistry and Molecular Biology, 95 Grasslands Road, Valhalla, NY 10595, USA
    Curr Opin Investig Drugs 9:865-70. 2008
    ..This review describes the latest approaches used to develop drugs against smallpox...
  4. ncbi request reprint Exploring DNA topoisomerases as targets of novel therapeutic agents in the treatment of infectious diseases
    Y C Tse-Dinh
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Infect Disord Drug Targets 7:3-9. 2007
    ..These new developments of DNA topoisomerases as targets of novel therapeutic agents being reviewed here represent excellent opportunities for drug discovery in the treatment of infectious diseases...
  5. ncbi request reprint DNA supercoiling and bacterial adaptation: thermotolerance and thermoresistance
    Y C Tse-Dinh
    Dept of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595, USA
    Trends Microbiol 5:323-6. 1997
    ..This role in heat shock has been elucidated by genetic studies on the Escherichia coli topA gene and its sigma 32-dependent promoter, P1. Other studies have shown that certain gyrA mutants have increased thermoresistance...
  6. pmc Increased sensitivity to oxidative challenges associated with topA deletion in Escherichia coli
    Y C Tse-Dinh
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Bacteriol 182:829-32. 2000
    ..This effect on oxidative challenge response represents a new role for E. coli DNA topoisomerase I in addition to prevention of excessive negative supercoiling of DNA...
  7. pmc Bacterial cell killing mediated by topoisomerase I DNA cleavage activity
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 280:38489-95. 2005
    ..Small molecules that induce similar perturbation in the enzyme-DNA complex should be candidates as leads for novel antibacterial agents...
  8. pmc The role of the Zn(II) binding domain in the mechanism of E. coli DNA topoisomerase I
    Adriana Ahumada
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY, USA
    BMC Biochem 3:13. 2002
    ..The 67 kDa N-terminal domain (Top67) has the active site tyrosine for DNA cleavage but cannot relax negatively supercoiled DNA. We analyzed the role of the ZD domain in the enzyme mechanism...
  9. pmc Mutation adjacent to the active site tyrosine can enhance DNA cleavage and cell killing by the TOPRIM Gly to Ser mutant of bacterial topoisomerase I
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Nucleic Acids Res 36:1017-25. 2008
    ....
  10. ncbi request reprint Site-directed mutagenesis of residues involved in G Strand DNA binding by Escherichia coli DNA topoisomerase I
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 279:39207-13. 2004
    ..Mutation of Ser-192 did not affect DNA binding and cleavage but nevertheless decreased the overall rate of relaxation of supercoiled DNA probably because of its participation in a later step of the reaction pathway...
  11. ncbi request reprint RNase H overproduction allows the expression of stress-induced genes in the absence of topoisomerase I
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    FEMS Microbiol Lett 221:237-42. 2003
    ..Topoisomerase I is likely required during stress response for preventing accumulation of transcription-driven hypernegative supercoiling and R-loop formation at induced stress genes loci...
  12. pmc The strictly conserved Arg-321 residue in the active site of Escherichia coli topoisomerase I plays a critical role in DNA rejoining
    Gagandeep Narula
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 286:18673-80. 2011
    ..Small molecules that can interfere or distort the enzyme-DNA interactions required for DNA rejoining by bacterial type IA topoisomerases could be developed into novel antibacterial drugs...
  13. ncbi request reprint Direct interaction between Escherichia coli RNA polymerase and the zinc ribbon domains of DNA topoisomerase I
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 278:30705-10. 2003
    ....
  14. ncbi request reprint Compounds with antibacterial activity that enhance DNA cleavage by bacterial DNA topoisomerase I
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    J Antimicrob Chemother 59:640-5. 2007
    ..Compounds that act in this mechanism against type IA topoisomerase have not been identified previously and could be leads for development of a new class of antibacterial agents...
  15. pmc Analysis of RuvABC and RecG involvement in the escherichia coli response to the covalent topoisomerase-DNA complex
    Jeanette H Sutherland
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Bacteriol 192:4445-51. 2010
    ....
  16. pmc The DNA relaxation activity and covalent complex accumulation of Mycobacterium tuberculosis topoisomerase I can be assayed in Escherichia coli: application for identification of potential FRET-dye labeling sites
    Gagandeep Narula
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    BMC Biochem 11:41. 2010
    ....
  17. pmc Experimental and computational investigations of Ser10 and Lys13 in the binding and cleavage of DNA substrates by Escherichia coli DNA topoisomerase I
    Daniel Strahs
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Nucleic Acids Res 34:1785-97. 2006
    ....
  18. ncbi request reprint Flexibility at Gly-194 is required for DNA cleavage and relaxation activity of Escherichia coli DNA topoisomerase I
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 279:8648-54. 2004
    ....
  19. pmc Topoisomerase I function during Escherichia coli response to antibiotics and stress enhances cell killing from stabilization of its cleavage complex
    I Fen Liu
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    J Antimicrob Chemother 66:1518-24. 2011
    ....
  20. ncbi request reprint Effect of the deletion of the sigma 32-dependent promoter (P1) of the Escherichia coli topoisomerase I gene on thermotolerance
    H Qi
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595, USA
    Mol Microbiol 21:703-11. 1996
    ..A model is proposed in order to unify these observations...
  21. pmc SOS induction by stabilized topoisomerase IA cleavage complex occurs via the RecBCD pathway
    Jeanette H Sutherland
    Department of Biochemistry and Molecular Biology, Basic Science Building, New York Medical College, Valhalla, NY 10595, USA
    J Bacteriol 190:3399-403. 2008
    ..The single-stranded break associated with mutant topoisomerase I cleavage complex is converted to double-stranded break, which then is processed by the RecBCD pathway, followed by association of RecA with the single-stranded DNA...
  22. pmc Isolation and quantitation of topoisomerase complexes accumulated on Escherichia coli chromosomal DNA
    Sandra Aedo
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, USA
    Antimicrob Agents Chemother 56:5458-64. 2012
    ....
  23. pmc Hydroxyl radicals are involved in cell killing by the bacterial topoisomerase I cleavage complex
    I Fen Liu
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Bacteriol 191:5315-9. 2009
    ..The presence of the Fe(2+) chelator 2,2'-dipyridyl and an iscS mutation affecting Fe-S cluster formation protect against topoisomerase I cleavage complex-mediated cell killing...
  24. pmc Inhibition of Mg2+ binding and DNA religation by bacterial topoisomerase I via introduction of an additional positive charge into the active site region
    Elena P Sorokin
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    Nucleic Acids Res 36:4788-96. 2008
    ..This approach would be similar to the inhibition of divalent ion dependent strand transfer by HIV integrase in antiviral therapy...
  25. pmc Asp-to-Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, Basic Science Building, New York Medical College, Valhalla, NY 10595, USA
    J Mol Biol 385:558-67. 2009
    ..The extreme sensitivity of the first TOPRIM position suggested that this might be a useful site for binding of small molecules that could act as topoisomerase poisons...
  26. pmc Identification of anziaic acid, a lichen depside from Hypotrachyna sp., as a new topoisomerase poison inhibitor
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, United States of America
    PLoS ONE 8:e60770. 2013
    ..This is the first report of a depside with activity as a topoisomerase poison inhibitor and demonstrates the potential of this class of natural products as a source for new antibacterial and anticancer compounds...
  27. pmc Loss of topoisomerase I function affects the RpoS-dependent and GAD systems of acid resistance in Escherichia coli
    Natalee Stewart
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, 10595, USA
    Microbiology 151:2783-91. 2005
    ..The effect of the topA mutation could be suppressed by an hns mutation, so topoisomerase I might be required to counteract the effect of H-NS protein on gene expression, in addition to its influence on RpoS-dependent transcription...
  28. pmc Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR
    I Fen Liu
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    BMC Microbiol 11:261. 2011
    ..The oxidative cell death pathway has also been shown to be involved in the lethality following accumulation of cleavage complex formed by bacterial topoisomerase I with mutations that result in defective DNA religation...
  29. pmc Residues of E. coli topoisomerase I conserved for interaction with a specific cytosine base to facilitate DNA cleavage
    Gagandeep Narula
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Nucleic Acids Res 40:9233-43. 2012
    ....
  30. pmc SbcCD-mediated processing of covalent gyrase-DNA complex in Escherichia coli
    Sandra Aedo
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, USA
    Antimicrob Agents Chemother 57:5116-9. 2013
    ..However, SbcCD activity protected cells against the bactericidal action of nalidixic acid but not ciprofloxacin. ..
  31. ncbi request reprint Topoisomerase function during bacterial responses to environmental challenge
    Shan Rui
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Front Biosci 8:d256-63. 2003
    ....
  32. pmc Crystal structure of a covalent intermediate in DNA cleavage and rejoining by Escherichia coli DNA topoisomerase I
    Zhongtao Zhang
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, NY 10595, USA
    Proc Natl Acad Sci U S A 108:6939-44. 2011
    ..This structure of the covalent intermediate provides the basis for the design of novel antibiotics that can trap the enzyme after formation of the covalent complex...
  33. ncbi request reprint Zinc (II) coordination in Escherichia coli DNA topoisomerase I is required for cleavable complex formation with DNA
    Y C Tse-Dinh
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595
    J Biol Chem 266:14317-20. 1991
    ..It is proposed that zinc(II) coordination in E. coli DNA topoisomerase I is required for the transition of the noncovalent complex with DNA to the cleavable state...
  34. ncbi request reprint Evaluation of topotecan and etoposide for non-Hodgkin lymphoma: correlation of topoisomerase-DNA complex formation with clinical response
    R R Kancherla
    Zalmen A Arlin Cancer Institute, Division of Oncology Hematology, Department of Medicine, Rm 250 Munger Pavilion, New York Medical College, Valhalla, NY 10595, USA
    Cancer 91:463-71. 2001
    ..The authors report Phase I data of topotecan and etoposide combination for patients with recurrent or refractory non-Hodgkin lymphoma and correlation of topoisomerase-DNA complex formation to clinical response...
  35. ncbi request reprint Increased thermosensitivity associated with topoisomerase I deletion and promoter mutations in Escherichia coli
    H Qi
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595, USA
    FEMS Microbiol Lett 178:141-6. 1999
    ..Two other E. coli mutants lacking topA were > 100 times more sensitive to high temperature than their wild-type isogenic strains...
  36. ncbi request reprint Regulation of Escherichia coli topA gene transcription: involvement of a sigmaS-dependent promoter
    H Qi
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595, USA
    J Mol Biol 267:481-9. 1997
    ..The utilization of multiple sigma factors for transcription initiation of topA could be important for adaptation of E. coli to change in growth conditions...
  37. pmc Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol
    Thirunavukkarasu Annamalai
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, USA
    BMC Biochem 10:18. 2009
    ..It shares a common transesterification domain with other type IA DNA topoisomerases. There is, however, no homology between the C-terminal DNA binding domains of Escherichia coli and M. tuberculosis DNA topoisomerase I proteins...
  38. pmc Inhibition of Zn(II) Binding Type IA Topoisomerases by Organomercury Compounds and Hg(II)
    Bokun Cheng
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, United States of America
    PLoS ONE 10:e0120022. 2015
    ..The Zn(II) binding domains found in human Top3α and Top3β may be potential targets of toxic metals and organometallic complexes, with potential consequence on genomic stability and development. ..
  39. ncbi request reprint Binding of Zn(II) to Escherichia coli DNA topoisomerase I
    C X Zhu
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595
    Biochem Mol Biol Int 33:195-204. 1994
    ..Digestion with carboxypeptidase Y further demonstrated that the folding of the zinc binding region itself was altered upon Zn(II) removal...
  40. ncbi request reprint Differential expression of human topoisomerase IIIalpha during the cell cycle progression in HL-60 leukemia cells and human peripheral blood lymphocytes
    C W Lin
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    Exp Cell Res 256:225-36. 2000
    ..Its cellular level appeared to be under control during the cell cycle progression of normal lymphocytes, but was found to be deregulated in HL-60 cells which may be associated with the tumor transformed cell phenotypes...
  41. ncbi request reprint RNase HI overproduction is required for efficient full-length RNA synthesis in the absence of topoisomerase I in Escherichia coli
    Imad Baaklini
    Departement de Microbiologie et Immunologie, Universite de Montreal, C P 6128, Succ Centre Ville, Montreal, P Quebec, Canada, H3C 3J7
    Mol Microbiol 54:198-211. 2004
    ..All together, our results suggest that excess negative supercoiling promotes the formation of R-loops, which, in turn, inhibit RNA synthesis...

Research Grants1

  1. Development of a HTS system:topoisomerase targets (RMI)
    Yuk Ching Tse Dinh; Fiscal Year: 2004
    ..The screening system should also be applicable for targeting topoisomerases in pathogenic bacteria relevant for biodefense. ..