Alexander Serganov

Summary

Affiliation: New York University School of Medicine
Country: USA

Publications

  1. pmc Metabolite recognition principles and molecular mechanisms underlying riboswitch function
    Alexander Serganov
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York 10016, USA
    Annu Rev Biophys 41:343-70. 2012
  2. pmc A decade of riboswitches
    Alexander Serganov
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA
    Cell 152:17-24. 2013
  3. pmc Amino acid recognition and gene regulation by riboswitches
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Biochim Biophys Acta 1789:592-611. 2009
  4. pmc Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formation
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Struct Mol Biol 12:218-24. 2005
  5. pmc Ribozymes, riboswitches and beyond: regulation of gene expression without proteins
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Rev Genet 8:776-90. 2007
  6. pmc Structure-function studies of FMRP RGG peptide recognition of an RNA duplex-quadruplex junction
    Anh Tuân Phan
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Struct Mol Biol 18:796-804. 2011
  7. doi request reprint Preparation and crystallization of riboswitch-ligand complexes
    Olga Pikovskaya
    Memorial Sloan Kettering Cancer Center, Department of Structural Biology, New York, NY 10021, USA
    Methods Mol Biol 540:115-28. 2009
  8. pmc Structural insights into amino acid binding and gene control by a lysine riboswitch
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 455:1263-7. 2008
  9. pmc Structural insights into ligand recognition by a sensing domain of the cooperative glycine riboswitch
    Lili Huang
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Mol Cell 40:774-86. 2010
  10. doi request reprint Crystal structure reveals specific recognition of a G-quadruplex RNA by a β-turn in the RGG motif of FMRP
    Nikita Vasilyev
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016
    Proc Natl Acad Sci U S A 112:E5391-400. 2015

Collaborators

Detail Information

Publications25

  1. pmc Metabolite recognition principles and molecular mechanisms underlying riboswitch function
    Alexander Serganov
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York 10016, USA
    Annu Rev Biophys 41:343-70. 2012
    ..These studies have greatly facilitated the dissection of molecular mechanisms underlying riboswitch action and should in turn guide the anticipated development of tools for manipulating gene regulatory circuits...
  2. pmc A decade of riboswitches
    Alexander Serganov
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA
    Cell 152:17-24. 2013
    ....
  3. pmc Amino acid recognition and gene regulation by riboswitches
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Biochim Biophys Acta 1789:592-611. 2009
    ..We also discuss the contribution of lysine riboswitches to antibiotic resistance and outline potential applications of riboswitches in biotechnology and medicine...
  4. pmc Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formation
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Struct Mol Biol 12:218-24. 2005
    ..We observe structural parallels in the independently evolved catalytic pocket architectures for ribozyme- and antibody-catalyzed Diels-Alder carbon-carbon bond-forming reactions...
  5. pmc Ribozymes, riboswitches and beyond: regulation of gene expression without proteins
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Rev Genet 8:776-90. 2007
    ..These findings have implications for understanding how cellular functions might have evolved from RNA-based origins...
  6. pmc Structure-function studies of FMRP RGG peptide recognition of an RNA duplex-quadruplex junction
    Anh Tuân Phan
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Struct Mol Biol 18:796-804. 2011
    ..These findings on FMRP RGG domain recognition by a combination of G-quadruplex and surrounding RNA sequences have implications for the recognition of other genomic G-rich RNAs...
  7. doi request reprint Preparation and crystallization of riboswitch-ligand complexes
    Olga Pikovskaya
    Memorial Sloan Kettering Cancer Center, Department of Structural Biology, New York, NY 10021, USA
    Methods Mol Biol 540:115-28. 2009
    ..The chapter describes the principles of construct design, in vitro transcription, RNA purification, complex formation, and crystallization screening utilized during the successful crystallization of several riboswitches...
  8. pmc Structural insights into amino acid binding and gene control by a lysine riboswitch
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 455:1263-7. 2008
    ....
  9. pmc Structural insights into ligand recognition by a sensing domain of the cooperative glycine riboswitch
    Lili Huang
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Mol Cell 40:774-86. 2010
    ....
  10. doi request reprint Crystal structure reveals specific recognition of a G-quadruplex RNA by a β-turn in the RGG motif of FMRP
    Nikita Vasilyev
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016
    Proc Natl Acad Sci U S A 112:E5391-400. 2015
    ..These results suggest that specific binding of cellular RNAs by FMRP may involve hydrogen bonding with RNA duplexes and that RNA duplex recognition can be a characteristic RNA binding feature for RGG motifs in other proteins. ..
  11. pmc Structural principles of nucleoside selectivity in a 2'-deoxyguanosine riboswitch
    Olga Pikovskaya
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Chem Biol 7:748-55. 2011
    ....
  12. pmc Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Chem Biol 11:1729-41. 2004
    ..Recognition specificity is associated with Watson-Crick pairing of the encapsulated adenine and guanine ligands with uridine and cytosine, respectively...
  13. pmc The long and the short of riboswitches
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Curr Opin Struct Biol 19:251-9. 2009
    ....
  14. pmc Argininamide binding arrests global motions in HIV-1 TAR RNA: comparison with Mg2+-induced conformational stabilization
    Stephen W Pitt
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Mol Biol 338:7-16. 2004
    ..The combined conformational analysis of TAR under three states reveals that ligands and divalent ions can stabilize similar RNA global conformations through distinct interactions involving different hydrogen bond alignments in the RNA...
  15. pmc Coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 458:233-7. 2009
    ..Our studies also explain the effects of spontaneous and antibiotic-induced deregulatory mutations and provided molecular insights into FMN-based control of gene expression in normal and riboflavin-overproducing bacterial strains...
  16. doi request reprint Structural and Dynamic Basis for Low-Affinity, High-Selectivity Binding of L-Glutamine by the Glutamine Riboswitch
    Aiming Ren
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Cell Rep 13:1800-13. 2015
    ..We propose that an open ligand-binding pocket combined with a high conformational penalty for forming the ligand-bound state provide mechanisms for reducing binding affinity while retaining high selectivity. ..
  17. pmc Long-range pseudoknot interactions dictate the regulatory response in the tetrahydrofolate riboswitch
    Lili Huang
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 108:14801-6. 2011
    ..Given that modern antibiotics target folate metabolism, the THF riboswitch structure provides insights on mechanistic aspects of riboswitch function and may help in manipulating THF levels in pathogenic bacteria...
  18. pmc Molecular recognition and function of riboswitches
    Alexander Serganov
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 550 First Ave, MSB 393, New York, NY 10016, USA
    Curr Opin Struct Biol 22:279-86. 2012
    ..These studies have greatly improved our understanding of molecular events in riboswitch-mediated gene expression control and provided the molecular basis for intervention into riboswitch-controlled genetic circuits...
  19. doi request reprint Structural insights into ligand binding and gene expression control by an adenosylcobalamin riboswitch
    Alla Peselis
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York, USA
    Nat Struct Mol Biol 19:1182-4. 2012
    ....
  20. ncbi request reprint Determination of riboswitch structures: light at the end of the tunnel?
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA
    RNA Biol 7:98-103. 2010
    ....
  21. pmc Towards deciphering the principles underlying an mRNA recognition code
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Curr Opin Struct Biol 18:120-9. 2008
    ..In this review, we have highlighted recent structural studies that illustrate the diversity of recognition principles used by mRNA binders for timely and specific targeting and processing of the message...
  22. doi request reprint Cooperativity, allostery and synergism in ligand binding to riboswitches
    Alla Peselis
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Biochimie 117:100-9. 2015
    ..Here we present an overview of the biochemical, genetic, and structural studies of riboswitches with a major focus on complex regulatory mechanisms and biological principles utilized by riboswitches for such genetic modulation. ..
  23. doi request reprint Preparation and Crystallization of Riboswitches
    Alla Peselis
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, 10016, USA
    Methods Mol Biol 1320:21-36. 2016
    ..The methodology was refined on crystallographic studies of several riboswitches and can be employed for other types of RNA molecules. ..
  24. pmc Structure and function of pseudoknots involved in gene expression control
    Alla Peselis
    Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, USA
    Wiley Interdiscip Rev RNA 5:803-22. 2014
    ....
  25. pmc Ribosomal protein S15 represses its own translation via adaptation of an rRNA-like fold within its mRNA
    Alexander Serganov
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    EMBO J 22:1898-908. 2003
    ..Our results highlight an astonishing plasticity of mRNA in its ability to adapt to evolutionary constraints, that contrasts with the extreme conservation of the rRNA-binding site...