James C Samuelson

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. doi request reprint Recent developments in difficult protein expression: a guide to E. coli strains, promoters, and relevant host mutations
    James C Samuelson
    New England Biolabs, Inc, Ipswich, MA, USA
    Methods Mol Biol 705:195-209. 2011
  2. pmc Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variants
    James C Samuelson
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 34:796-805. 2006
  3. pmc The isolation of strand-specific nicking endonucleases from a randomized SapI expression library
    James C Samuelson
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 32:3661-71. 2004
  4. pmc Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA
    Penghua Zhang
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 69:226-34. 2010
  5. pmc Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI
    Shuang yong Xu
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:4608-18. 2007
  6. ncbi request reprint Directed evolution of restriction endonuclease BstYI to achieve increased substrate specificity
    James C Samuelson
    New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA
    J Mol Biol 319:673-83. 2002
  7. pmc Engineering Escherichia coli BL21(DE3) derivative strains to minimize E. coli protein contamination after purification by immobilized metal affinity chromatography
    Carine Robichon
    New England Biolabs, Inc, Gene Expression Division, 240 County Road, Ipswich, MA 01938, USA
    Appl Environ Microbiol 77:4634-46. 2011
  8. ncbi request reprint Engineering strand-specific DNA nicking enzymes from the type IIS restriction endonucleases BsaI, BsmBI, and BsmAI
    Zhenyu Zhu
    New England Biolabs, Inc 32 Tozer Road, Beverly, MA 01915, USA
    J Mol Biol 337:573-83. 2004
  9. pmc Rational design of a fusion partner for membrane protein expression in E. coli
    Jianying Luo
    New England Biolabs, Inc Gene Expression Division, Ipswich, Massachusetts 01938, USA
    Protein Sci 18:1735-44. 2009
  10. ncbi request reprint BstYI bound to noncognate DNA reveals a "hemispecific" complex: implications for DNA scanning
    Sharon A Townson
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
    Structure 15:449-59. 2007

Collaborators

  • Shuang yong Xu
  • Siu Hong Chan
  • Richard D Morgan
  • Eva Scheuring Vanamee
  • Sharon A Townson
  • Jianying Luo
  • Aneel K Aggarwal
  • Carine Robichon
  • Penghua Zhang
  • Zhenyu Zhu
  • Minyong Chen
  • Jack S Benner
  • Thomas B Causey
  • Timothy M Schramm
  • Priscilla Hiu Mei Too
  • David C Schwartz
  • Stephanie Doucette
  • Dan Forrest
  • Stefan Bäckström
  • Konstantinos D Potamousis
  • Tamas Vincze
  • Julie Choulet
  • Yongming Bao
  • Jing Zhou
  • Andrew Dore
  • Thomas A Edwards
  • Carlos R Escalante
  • Fenglei Jiang
  • Ross E Dalbey
  • Matthias Muller
  • Andreas Kuhn

Detail Information

Publications13

  1. doi request reprint Recent developments in difficult protein expression: a guide to E. coli strains, promoters, and relevant host mutations
    James C Samuelson
    New England Biolabs, Inc, Ipswich, MA, USA
    Methods Mol Biol 705:195-209. 2011
    ..This chapter discusses the properties of many of the E. coli strains available for protein expression in order to facilitate the choice of the best expression host for a particular protein of interest...
  2. pmc Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variants
    James C Samuelson
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 34:796-805. 2006
    ..2 x 10(4) U/mg, a 32-fold increase over variant E156K. A comprehensive analysis indicates that the cleavage specificity of M91V/E156K is relaxed to a small set of 8 bp substrates while retaining activity towards the NotI sequence...
  3. pmc The isolation of strand-specific nicking endonucleases from a randomized SapI expression library
    James C Samuelson
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 32:3661-71. 2004
    ..The nature of the amino acid substitutions found in the selected variants provides evidence that SapI may possess two active sites per monomer. This work presents a framework for establishing the mechanism of SapI DNA cleavage...
  4. pmc Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA
    Penghua Zhang
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 69:226-34. 2010
    ..Restriction mapping and run-off DNA sequencing of digested products from the partially purified enzyme indicated that it is an EarI isoschizomer with 6-bp recognition, which we named CatHI (CTCTTC N1/N4)...
  5. pmc Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI
    Shuang yong Xu
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:4608-18. 2007
    ..Top-strand specific nicking variants, Nt.BsrDI and Nt.BtsI, were successfully engineered by combining the catalytic-deficient B subunit with wild-type A subunit...
  6. ncbi request reprint Directed evolution of restriction endonuclease BstYI to achieve increased substrate specificity
    James C Samuelson
    New England Biolabs, 32 Tozer Road, Beverly, MA 01915, USA
    J Mol Biol 319:673-83. 2002
    ..Moreover, cleavage of the GGATCC sequence is no longer detected. This study provides further evidence that laboratory evolution strategies offer a powerful alternative to structure-guided protein design...
  7. pmc Engineering Escherichia coli BL21(DE3) derivative strains to minimize E. coli protein contamination after purification by immobilized metal affinity chromatography
    Carine Robichon
    New England Biolabs, Inc, Gene Expression Division, 240 County Road, Ipswich, MA 01938, USA
    Appl Environ Microbiol 77:4634-46. 2011
    ..The "NiCo" strains uniquely complement existing methods for improving the purity of recombinant His-tagged protein...
  8. ncbi request reprint Engineering strand-specific DNA nicking enzymes from the type IIS restriction endonucleases BsaI, BsmBI, and BsmAI
    Zhenyu Zhu
    New England Biolabs, Inc 32 Tozer Road, Beverly, MA 01915, USA
    J Mol Biol 337:573-83. 2004
    ..This work provides strong evidence that some type IIS restriction endonucleases carry two separate active sites. When one of the active sites is inactivated, the type IIS restriction endonuclease may nick only one strand...
  9. pmc Rational design of a fusion partner for membrane protein expression in E. coli
    Jianying Luo
    New England Biolabs, Inc Gene Expression Division, Ipswich, Massachusetts 01938, USA
    Protein Sci 18:1735-44. 2009
    ..We anticipate that this optimized expression system will aid in the isolation and study of various recombinant forms of membrane-associated protein...
  10. ncbi request reprint BstYI bound to noncognate DNA reveals a "hemispecific" complex: implications for DNA scanning
    Sharon A Townson
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
    Structure 15:449-59. 2007
    ..Taken together, the structure provides a snapshot of an enzyme in a "paused" intermediate state that may be part of a more general mechanism of scanning DNA...
  11. ncbi request reprint Implications for switching restriction enzyme specificities from the structure of BstYI bound to a BglII DNA sequence
    Sharon A Townson
    Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, New York 10029, USA
    Structure 13:791-801. 2005
    ..Taken together, the structure reveals a mechanism of degenerate DNA recognition and offers insights into the possibilities and limitations in altering specificities of closely related restriction enzymes...
  12. ncbi request reprint Crystal structure of BstYI at 1.85A resolution: a thermophilic restriction endonuclease with overlapping specificities to BamHI and BglII
    Sharon A Townson
    Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029, USA
    J Mol Biol 338:725-33. 2004
    ..This arm substructure may underlie the thermostability of BstYI. We identify putative DNA recognition residues and speculate as to how this enzyme achieves a "relaxed" DNA specificity...
  13. ncbi request reprint Direct interaction of YidC with the Sec-independent Pf3 coat protein during its membrane protein insertion
    Minyong Chen
    Department of Chemistry, Ohio State Biochemistry Program, and Protein Research Group, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 277:7670-5. 2002
    ..Our study demonstrates that YidC can directly interact with a Sec-independent membrane protein, and the role of YidC is at the stage of folding the Pf3 protein into a transmembrane configuration...