P Riggs

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. pmc SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm
    Julie Lobstein
    New England Biolabs, Ipswich, MA 01938, USA
    Microb Cell Fact 11:56. 2012
  2. pmc Mutations in maltose-binding protein that alter affinity and solubility properties
    Iris H Walker
    New England Biolabs, 240 County Rd, Ipswich, MA 01938 2723, USA
    Appl Microbiol Biotechnol 88:187-97. 2010
  3. ncbi request reprint Expression and purification of recombinant proteins by fusion to maltose-binding protein
    P Riggs
    New England Biolabs, Beverly, MA, USA
    Mol Biotechnol 15:51-63. 2000
  4. ncbi request reprint Expression and purification of maltose-binding protein fusions
    P Riggs
    New England Biolabs, Beverly, Massachusetts, USA
    Curr Protoc Mol Biol . 2001
  5. ncbi request reprint Introduction to expression by fusion protein vectors
    P Riggs
    New England Biolabs, Beverly, Massachusetts, USA
    Curr Protoc Mol Biol . 2001

Collaborators

  • Julie Lobstein
  • Iris H Walker
  • Mehmet Berkmen
  • Charlie A Emrich
  • Chris Jeans
  • Melinda Faulkner
  • Pei chung Hsieh

Detail Information

Publications5

  1. pmc SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm
    Julie Lobstein
    New England Biolabs, Ipswich, MA 01938, USA
    Microb Cell Fact 11:56. 2012
    ..Thus, new protein expression strains must be engineered and the parameters involved in producing disulfide bonded proteins must be understood...
  2. pmc Mutations in maltose-binding protein that alter affinity and solubility properties
    Iris H Walker
    New England Biolabs, 240 County Rd, Ipswich, MA 01938 2723, USA
    Appl Microbiol Biotechnol 88:187-97. 2010
    ..Some of the mutations we obtained can be rationalized based on the previous work, while others indicate new ways in which the function of MBP can be modified...
  3. ncbi request reprint Expression and purification of recombinant proteins by fusion to maltose-binding protein
    P Riggs
    New England Biolabs, Beverly, MA, USA
    Mol Biotechnol 15:51-63. 2000
    ..Vectors with or without the malE signal sequence can be used, to express the protein cytoplasmically for the highest level of production or periplasmically to help in proper folding of disulfide-bonded proteins...
  4. ncbi request reprint Expression and purification of maltose-binding protein fusions
    P Riggs
    New England Biolabs, Beverly, Massachusetts, USA
    Curr Protoc Mol Biol . 2001
    ..Additional support protocols detail two different chromatographic methods for separating the protein of interest from MBP after factor Xa cleavage...
  5. ncbi request reprint Introduction to expression by fusion protein vectors
    P Riggs
    New England Biolabs, Beverly, Massachusetts, USA
    Curr Protoc Mol Biol . 2001
    ..g., heat stability) can be exploited to allow selective purification of the fusion protein. Often, proteins fused to these carriers can be separated from the bulk of intracellular contaminants by taking advantage of special attributes...