S Pestka

Summary

Affiliation: New Jersey
Country: USA

Publications

  1. pmc Efficient co-expression of bicistronic proteins in mesenchymal stem cells by development and optimization of a multifunctional plasmid
    Christopher D Krause
    Department of Molecular Genetics, Microbiology, and Immunology, RWJMS UMDNJ, 675 Hoes Lane West, Piscataway, NJ 08854, USA
    Stem Cell Res Ther 2:15. 2011
  2. pmc AUF1 isoform-specific regulation of anti-inflammatory IL10 expression in monocytes
    Srijata Sarkar
    Department of Molecular Genetics, Microbiology, and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Interferon Cytokine Res 28:679-91. 2008
  3. ncbi request reprint Purification and cloning of interferon alpha
    S Pestka
    Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Curr Top Microbiol Immunol 316:23-37. 2007
  4. ncbi request reprint The interferons: 50 years after their discovery, there is much more to learn
    Sidney Pestka
    Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Biol Chem 282:20047-51. 2007
  5. ncbi request reprint hTid-1, a human DnaJ protein, modulates the interferon signaling pathway
    S Sarkar
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 276:49034-42. 2001
  6. ncbi request reprint Identification, cloning, and characterization of a novel soluble receptor that binds IL-22 and neutralizes its activity
    S V Kotenko
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Immunol 166:7096-103. 2001
  7. ncbi request reprint Hsl7p, the yeast homologue of human JBP1, is a protein methyltransferase
    J H Lee
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854 5635, USA
    Biochem Biophys Res Commun 274:105-11. 2000
  8. ncbi request reprint Other kinases can substitute for Jak2 in signal transduction by interferon-gamma
    S V Kotenko
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 271:17174-82. 1996
  9. pmc Human cytomegalovirus harbors its own unique IL-10 homolog (cmvIL-10)
    S V Kotenko
    Department of Molecular Genetics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
    Proc Natl Acad Sci U S A 97:1695-700. 2000
  10. ncbi request reprint Genetic mapping of the gene for the mouse interferon-gamma receptor signaling subunit to the distal end of chromosome 16
    T M Mariano
    Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854 5635, USA
    Mamm Genome 7:321-2. 1996

Research Grants

  1. INTERFERON ALPHA/BETA RECEPTORS
    Sidney Pestka; Fiscal Year: 2004
  2. INTERLEUKIN 10 RECEPTOR COMPLEX
    Sidney Pestka; Fiscal Year: 2003
  3. INTERFERON ALPHA/BETA RECEPTORS
    Sidney Pestka; Fiscal Year: 1999
  4. Receptor-Signaling Protein Interactions in Real Time
    Sidney Pestka; Fiscal Year: 2007

Collaborators

Detail Information

Publications35

  1. pmc Efficient co-expression of bicistronic proteins in mesenchymal stem cells by development and optimization of a multifunctional plasmid
    Christopher D Krause
    Department of Molecular Genetics, Microbiology, and Immunology, RWJMS UMDNJ, 675 Hoes Lane West, Piscataway, NJ 08854, USA
    Stem Cell Res Ther 2:15. 2011
    ..Many vectors exist to fulfill this purpose, but their transfection efficiency and resulting expression levels vary considerably...
  2. pmc AUF1 isoform-specific regulation of anti-inflammatory IL10 expression in monocytes
    Srijata Sarkar
    Department of Molecular Genetics, Microbiology, and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Interferon Cytokine Res 28:679-91. 2008
    ..Thus, the p40 AUF1 isoform selectively plays a critical, positive role in IL10 expression upon LPS exposure...
  3. ncbi request reprint Purification and cloning of interferon alpha
    S Pestka
    Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Curr Top Microbiol Immunol 316:23-37. 2007
    ..This paper reviews the history of the purification of human leukocyte IFN, the cloning of the IFN-alphas, and the current state of knowledge of human interferon alpha genes and proteins...
  4. ncbi request reprint The interferons: 50 years after their discovery, there is much more to learn
    Sidney Pestka
    Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Biol Chem 282:20047-51. 2007
    ..This brief review focuses on the discovery and purification of interferons, cloning of human IFN-alpha and IFN-beta, interferon receptors, activities and therapeutic uses of interferons, and the side effects of interferons...
  5. ncbi request reprint hTid-1, a human DnaJ protein, modulates the interferon signaling pathway
    S Sarkar
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 276:49034-42. 2001
    ..Furthermore, both hTid-1(S) and hTid-1(L) can modulate IFN-gamma-mediated transcriptional activity...
  6. ncbi request reprint Identification, cloning, and characterization of a novel soluble receptor that binds IL-22 and neutralizes its activity
    S V Kotenko
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Immunol 166:7096-103. 2001
    ..Thus, the soluble receptor designated IL-22BP inhibits IL-22 activity by binding IL-22 and blocking its interaction with the cell surface IL-22R complex...
  7. ncbi request reprint Hsl7p, the yeast homologue of human JBP1, is a protein methyltransferase
    J H Lee
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854 5635, USA
    Biochem Biophys Res Commun 274:105-11. 2000
    ..Therefore, we conclude the yeast protein Hsl7p is a sequence and functional homologue of JBP1. These data provide evidence for an intricate link between protein methylation and macroscopic changes in yeast morphology...
  8. ncbi request reprint Other kinases can substitute for Jak2 in signal transduction by interferon-gamma
    S V Kotenko
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 271:17174-82. 1996
    ..The results may suggest that the Jaks do not contribute to the specificity of signal transduction in the Jak-Stat pathway to the same degree as Stats...
  9. pmc Human cytomegalovirus harbors its own unique IL-10 homolog (cmvIL-10)
    S V Kotenko
    Department of Molecular Genetics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
    Proc Natl Acad Sci U S A 97:1695-700. 2000
    ..We demonstrated that cmvIL-10 is expressed in CMV-infected cells. Thus, expression of cmvIL-10 extends the range of counter measures developed by CMV to circumvent detection and destruction by the host immune system...
  10. ncbi request reprint Genetic mapping of the gene for the mouse interferon-gamma receptor signaling subunit to the distal end of chromosome 16
    T M Mariano
    Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854 5635, USA
    Mamm Genome 7:321-2. 1996
  11. ncbi request reprint The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity
    B P Pollack
    Department of Molecular Genetics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 274:31531-42. 1999
    ..This report provides a clue to the biochemical function for this conserved protein and suggests that protein methyltransferases may have a role in cellular signaling...
  12. ncbi request reprint Identification of the functional interleukin-22 (IL-22) receptor complex: the IL-10R2 chain (IL-10Rbeta ) is a common chain of both the IL-10 and IL-22 (IL-10-related T cell-derived inducible factor, IL-TIF) receptor complexes
    S V Kotenko
    Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 276:2725-32. 2001
    ....
  13. ncbi request reprint Identification and sequence of an accessory factor required for activation of the human interferon gamma receptor
    J Soh
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854 5635
    Cell 76:793-802. 1994
    ..We conclude that this accessory factor belongs to a family of such accessory factors responsible for different actions of IFN-gamma...
  14. ncbi request reprint Chimeric erythropoietin-interferon gamma receptors reveal differences in functional architecture of intracellular domains for signal transduction
    G Muthukumaran
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 272:4993-9. 1997
    ..EpoR/gammaR2 heterodimer is a fully functional receptor complex. The results demonstrate that the configuration of the extracellular domains influences the architecture of the intracellular domains...
  15. ncbi request reprint Evolution of the Class 2 cytokines and receptors, and discovery of new friends and relatives
    Christopher D Krause
    Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    Pharmacol Ther 106:299-346. 2005
    ..In addition, the genomic analysis led to the discovery of some new members of this family...
  16. pmc Chaperone Hsp27 modulates AUF1 proteolysis and AU-rich element-mediated mRNA degradation
    Anna M Knapinska
    Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane West, Piscataway, NJ 08854, USA
    Mol Cell Biol 31:1419-31. 2011
    ..Our results suggest that the p38 MAP kinase (MAPK)-MK2-Hsp27 signaling axis may target AUF1 destruction by proteasomes, thereby promoting ARE mRNA stabilization...
  17. ncbi request reprint The human interferon alpha species and receptors
    S Pestka
    Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854 5635, USA
    Biopolymers 55:254-87. 2000
    ..This paper reviews the history of the purification of human leukocyte IFN and key aspects of our current state of knowledge of human interferon alpha genes, proteins, and receptors...
  18. ncbi request reprint The structure of the gene for the second chain of the human interferon-gamma receptor
    S Rhee
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 5635, USA
    J Biol Chem 271:28947-52. 1996
    ..Promoter activity of the 5'-flanking region was investigated with a luciferase reporter gene and the cytomegalovirus minimal promoter. Segments of the 5' region with promoter activity were identified...
  19. ncbi request reprint Interleukin-10 and related cytokines and receptors
    Sidney Pestka
    Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    Annu Rev Immunol 22:929-79. 2004
    ..Further investigation of these critical intercellular signaling molecules will provide important information to enable these proteins to be used more extensively in therapy for a variety of diseases...
  20. ncbi request reprint Protein arginine methyltransferases: evolution and assessment of their pharmacological and therapeutic potential
    Christopher D Krause
    Department of Molecular Genetics, Microbiology, and Immunology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Pharmacol Ther 113:50-87. 2007
    ..Nevertheless, development of pharmaceutical agents to control PRMT functions could lead to significant new targets...
  21. ncbi request reprint Historical developments in the research of interferon receptors
    Christopher D Krause
    Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School The University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    Cytokine Growth Factor Rev 18:473-82. 2007
    ..In this mini-review, we take a historical perspective at the major events in the characterization of interferon receptors, discussing interesting results that still need to be explained...
  22. ncbi request reprint Increased interleukin-10 mRNA stability in melanoma cells is associated with decreased levels of A + U-rich element binding factor AUF1
    Gary Brewer
    Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School UMDNJ, Piscataway, NJ 08854, USA
    J Interferon Cytokine Res 23:553-64. 2003
    ..Together, these data suggest a mechanism whereby reduced cytoplasmic levels of AUF1 in MNT1 melanoma cells may lead to IL-10 overexpression, with deleterious consequences for tumor surveillance and rejection...
  23. ncbi request reprint Molecular characterization of the murine interferon gamma receptor cDNA
    C S Kumar
    Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854 5635
    J Biol Chem 264:17939-46. 1989
    ....
  24. pmc Identification and functional characterization of a second chain of the interleukin-10 receptor complex
    S V Kotenko
    Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
    EMBO J 16:5894-903. 1997
    ..Thus, the CRFB4 chain, which we designate as the IL-10R2 or IL-10Rbeta chain, serves as an accessory chain essential for the active IL-10 receptor complex and to initiate IL-10-induced signal transduction events...
  25. ncbi request reprint Elevated expression of ISG15 in tumor cells interferes with the ubiquitin/26S proteasome pathway
    Shyamal D Desai
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Cancer Res 66:921-8. 2006
    ..Together, our results suggest that the ISG15 pathway, which is deregulated during tumorigenesis, negatively regulates the ubiquitin/proteasome pathway by interfering with protein polyubiquitination/degradation...
  26. ncbi request reprint Preassembly and ligand-induced restructuring of the chains of the IFN-gamma receptor complex: the roles of Jak kinases, Stat1 and the receptor chains
    Christopher D Krause
    Department of Molecular Genetics, Microbiology and Immunology, The University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, 08854, USA
    Cell Res 16:55-69. 2006
    ..These results agree with a detailed model of the IFN-gamma receptor that requires the receptor chains to be pre-associated constitutively for the receptor to be active...
  27. ncbi request reprint Interferons, interferon-like cytokines, and their receptors
    Sidney Pestka
    Department of Molecular Genetics, Microbiology, and Immunology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854 5635, USA
    Immunol Rev 202:8-32. 2004
    ..The overall structure of the IFNs is put into perspective in relation to their receptors and functions...
  28. pmc Chaperone Hsp27, a novel subunit of AUF1 protein complexes, functions in AU-rich element-mediated mRNA decay
    Kristina S Sinsimer
    Department of Molecular Genetics, Microbiology and Immunology, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Cell Biol 28:5223-37. 2008
    ....
  29. pmc RNA-binding protein AUF1 regulates lipopolysaccharide-induced IL10 expression by activating IkappaB kinase complex in monocytes
    Srijata Sarkar
    Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854 5635, USA
    Mol Cell Biol 31:602-15. 2011
    ..Thus, p40(AUF1) regulates a critical node within the NF-κB signaling pathway to permit IL10 induction for the anti-inflammatory arm of an innate immune response...
  30. ncbi request reprint Site-specific 32P-labeling of cytokines, monoclonal antibodies, and other protein substrates for quantitative assays and therapeutic application
    William A Clark
    PBL Biomedical Laboratories, Piscataway, NJ 08854 5900, USA
    Biotechniques . 2002
    ..Most importantly 32P labeling of monoclonal antibodies or other therapeutic protein candidates has several significant advantages over radioiodination or chemical conjugation of heavy metal isotopes...
  31. ncbi request reprint Seeing the light: preassembly and ligand-induced changes of the interferon gamma receptor complex in cells
    Christopher D Krause
    Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School University of Medicine and Dentistry of New Jersey, Piscataway 08854 5635, USA
    Mol Cell Proteomics 1:805-15. 2002
    ..Application of this technology should lead to new rapid methods for high throughput screening and delineation of the interactome of cells...
  32. ncbi request reprint Interactions among the components of the interleukin-10 receptor complex
    Christopher D Krause
    Department of Molecular Genetics, Microbiology, and Immunology, the University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, NJ 08854, USA
    Biochem Biophys Res Commun 340:377-85. 2006
    ..The IL-10 receptor complex undergoes no major conformational changes when cells are treated with cellular or Epstein-Barr viral IL-10. Receptor complex preassembly may be an inherent feature of Class 2 cytokine receptor complexes...
  33. ncbi request reprint FBXO11/PRMT9, a new protein arginine methyltransferase, symmetrically dimethylates arginine residues
    Jeffry R Cook
    UMDNJ Robert Wood Johnson Medical School, Molecular Genetics and Immunology, 675 Hoes Lane, Rm 727, Piscataway, NJ 08854, USA
    Biochem Biophys Res Commun 342:472-81. 2006
    ..Structurally, this protein is distinct from all other known PRMTs implying that convergent evolution allowed this protein to develop the ability to methylate arginine residues and evolved elements conserved in PRMTs to accomplish this...
  34. ncbi request reprint Modulation of the activation of Stat1 by the interferon-gamma receptor complex
    Christopher D Krause
    Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, The University of Medicine and Dentistry of New Jersey, 675 Hoes Lane West, Piscataway, 08855, USA
    Cell Res 16:113-23. 2006
    ....
  35. ncbi request reprint A dance between interferon-alpha/beta and p53 demonstrates collaborations in tumor suppression and antiviral activities
    Sidney Pestka
    Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School UMDNJ, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Cancer Cell 4:85-7. 2003
    ....

Research Grants19

  1. INTERFERON ALPHA/BETA RECEPTORS
    Sidney Pestka; Fiscal Year: 2004
    ..Identification of all the pathways initiated through the Type I interferon receptor will provide an opportunity to develop new interferons and new approaches to treat many diseases. ..
  2. INTERLEUKIN 10 RECEPTOR COMPLEX
    Sidney Pestka; Fiscal Year: 2003
    ..Furthermore, because IL-10 is an important immunosuppressive cytokine in immune function such as in development of T-cell subsets, the knowledge gained should help us understand the mechanisms by which IL-10 mediates immunosuppression. ..
  3. INTERFERON ALPHA/BETA RECEPTORS
    Sidney Pestka; Fiscal Year: 1999
    ....
  4. Receptor-Signaling Protein Interactions in Real Time
    Sidney Pestka; Fiscal Year: 2007
    ..Further application of this technology should lead to new rapid methods for high throughput screening and for identifying protein-protein interactions in live cells in real-time. ..