F B Perler

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. pmc Splicing of the mycobacteriophage Bethlehem DnaB intein: identification of a new mechanistic class of inteins that contain an obligate block F nucleophile
    Kazuo Tori
    New England Biolabs, Ipswich, Massachusetts 01938, USA
    J Biol Chem 285:2515-26. 2010
  2. pmc Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues
    Manoj Cheriyan
    New England Biolabs, Inc, Ipswich, Massachusetts 01938, USA
    J Biol Chem 288:6202-11. 2013
  3. pmc The Arthrobacter species FB24 Arth_1007 (DnaB) intein is a pseudogene
    Kazuo Tori
    New England Biolabs, Inc, Ipswich, Massachusetts, United States of America
    PLoS ONE 6:e26361. 2011
  4. pmc Branched intermediate formation is the slowest step in the protein splicing reaction of the Ala1 KlbA intein from Methanococcus jannaschii
    Lana Saleh
    New England Biolabs, Ipswich, Massachusetts 01938, United States
    Biochemistry 50:10576-89. 2011
  5. ncbi request reprint Protein splicing mechanisms and applications
    Francine B Perler
    New England Biolabs, Ipswich, Massachusetts 01915, USA
    IUBMB Life 58:63. 2006
  6. ncbi request reprint Protein splicing mechanisms and applications
    Francine B Perler
    New England Biolabs, Ipswich, Massachusetts 01938 2723, USA
    IUBMB Life 57:469-76. 2005
  7. pmc Compilation and analysis of intein sequences
    F B Perler
    New England Biolabs Inc, Beverly, MA 01915, USA
    Nucleic Acids Res 25:1087-93. 1997
  8. pmc InBase: the Intein Database
    Francine B Perler
    New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 30:383-4. 2002
  9. ncbi request reprint Protein splicing and its applications
    F B Perler
    New England Biolabs Inc, Beverly, MA 01915, USA
    Curr Opin Biotechnol 11:377-83. 2000
  10. pmc InBase, the New England Biolabs Intein Database
    F B Perler
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 27:346-7. 1999

Collaborators

Detail Information

Publications26

  1. pmc Splicing of the mycobacteriophage Bethlehem DnaB intein: identification of a new mechanistic class of inteins that contain an obligate block F nucleophile
    Kazuo Tori
    New England Biolabs, Ipswich, Massachusetts 01938, USA
    J Biol Chem 285:2515-26. 2010
    ....
  2. pmc Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues
    Manoj Cheriyan
    New England Biolabs, Inc, Ipswich, Massachusetts 01938, USA
    J Biol Chem 288:6202-11. 2013
    ..Kinetic analysis showed splicing rates with the selected exteins that were as fast or faster than the native extein, refuting past assumptions that the naturally selected flanking extein sequences are optimal for splicing...
  3. pmc The Arthrobacter species FB24 Arth_1007 (DnaB) intein is a pseudogene
    Kazuo Tori
    New England Biolabs, Inc, Ipswich, Massachusetts, United States of America
    PLoS ONE 6:e26361. 2011
    ..This study restores the unity of the Class 3 intein signature sequence in active inteins by demonstrating that the Arsp-FB24 Arth_1007 intein is an inactive pseudogene...
  4. pmc Branched intermediate formation is the slowest step in the protein splicing reaction of the Ala1 KlbA intein from Methanococcus jannaschii
    Lana Saleh
    New England Biolabs, Ipswich, Massachusetts 01938, United States
    Biochemistry 50:10576-89. 2011
    ....
  5. ncbi request reprint Protein splicing mechanisms and applications
    Francine B Perler
    New England Biolabs, Ipswich, Massachusetts 01915, USA
    IUBMB Life 58:63. 2006
  6. ncbi request reprint Protein splicing mechanisms and applications
    Francine B Perler
    New England Biolabs, Ipswich, Massachusetts 01938 2723, USA
    IUBMB Life 57:469-76. 2005
    ..Recent advances include intein-mediated attachment of proteins to solid supports for microarray or western blot analysis, linking nucleic acids to proteins and controllable splicing, which converts inteins into molecular switches...
  7. pmc Compilation and analysis of intein sequences
    F B Perler
    New England Biolabs Inc, Beverly, MA 01915, USA
    Nucleic Acids Res 25:1087-93. 1997
    ....
  8. pmc InBase: the Intein Database
    Francine B Perler
    New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 30:383-4. 2002
    ..It presents general information as well as detailed data for each intein, including tabulated comparisons and a comprehensive bibliography. An intein-specific BLAST server is now available to assist in identifying new inteins...
  9. ncbi request reprint Protein splicing and its applications
    F B Perler
    New England Biolabs Inc, Beverly, MA 01915, USA
    Curr Opin Biotechnol 11:377-83. 2000
    ....
  10. pmc InBase, the New England Biolabs Intein Database
    F B Perler
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 27:346-7. 1999
    ..html), is a comprehensive on-line database that includes the Intein Registry, along with detailed information about each intein and its host protein, tabulated comparisons and a comprehensive bibliography including papers in press...
  11. ncbi request reprint Protein splicing and autoproteolysis mechanisms
    F B Perler
    New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Curr Opin Chem Biol 1:292-9. 1997
    ..Although such acyl rearrangements are thermodynamically unfavorable, their coupling to diverse types of self-catalyzed irreversible steps drives the protein rearrangements to completion...
  12. pmc InBase, the Intein Database
    F B Perler
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 28:344-5. 2000
    ..InBase presents general information as well as detailed data for each intein, including tabu-lated comparisons and a comprehensive bibliography...
  13. ncbi request reprint Protein splicing of inteins and hedgehog autoproteolysis: structure, function, and evolution
    F B Perler
    New England Biolabs, Inc, Beverly, Massachusetts 01915, USA
    Cell 92:1-4. 1998
  14. doi request reprint Protein splicing: A versatile tool for drug discovery
    Manoj Cheriyan
    New England Biolabs, Ipswich, MA 01938, USA
    Adv Drug Deliv Rev 61:899-907. 2009
    ..Inteins are rapidly becoming valuable tools for drug discovery and drug delivery...
  15. ncbi request reprint Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
    S Chong
    New England Biolabs, Beverly, MA 01915, USA
    Gene 192:271-81. 1997
    ..No exogenous proteolytic cleavage is needed. Furthermore, using this procedure, the purified free target protein can be specifically labeled at its C-terminus...
  16. ncbi request reprint Protein splicing in cis and in trans
    Lana Saleh
    New England Biolabs, 240 County Road, Ipswich, Massachusetts 01938, USA
    Chem Rec 6:183-93. 2006
    ..Understanding the protein splicing pathway has allowed scientists to harness inteins for numerous applications...
  17. ncbi request reprint Rescue of protein splicing activity from a Magnetospirillum magnetotacticum intein-like element
    M W Southworth
    New England Biolabs, 32 Tozer Road, Beverly, MA 01915, U S A
    Biochem Soc Trans 32:250-4. 2004
    ..Mutation of Tyr+1 to Phe or Ala indicated that the Tyr side-chain hydroxyl was not necessary for N-terminal cleavage. Protein splicing activity could be rescued by "reversion" of Tyr+1 to Cys...
  18. pmc Expanding the definition of class 3 inteins and their proposed phage origin
    Kazuo Tori
    New England Biolabs, Ipswich, MA 01938, USA
    J Bacteriol 193:2035-41. 2011
    ..Based on phylogenetic analysis, we propose that class 3 inteins arose from a single mutated intein that was spread by phage into predominantly helicase genes in various phages and their hosts...
  19. ncbi request reprint Creation of an artificial bifunctional intein by grafting a homing endonuclease into a mini-intein
    Marilena Fitzsimons Hall
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    J Mol Biol 323:173-9. 2002
    ..The function of each domain therefore remained unaffected by the presence of the other domain. This artificial fusion of the two domains is a potential novel mobile genetic element...
  20. pmc Bacteriophage-based genetic system for selection of nonsplicing inteins
    Isaac K O Cann
    New England Biolabs, Inc, Beverly, Massachusetts 01915, USA
    Appl Environ Microbiol 70:3158-62. 2004
    ..Development of genetic systems with the potential of identifying protein splicing inhibitors is a first step towards controlling proliferation of pathogenic microbes harboring inteins in essential proteins...
  21. ncbi request reprint Development of a positive genetic selection system for inhibition of protein splicing using mycobacterial inteins in Escherichia coli DNA gyrase subunit A
    Eric Adam
    New England Biolabs, Beverly, MA 01915, USA
    J Mol Microbiol Biotechnol 4:479-87. 2002
    ..Mutations were mapped onto a structure-based sequence alignment, which led to the rational prediction of a temperature-sensitive splicing mutation. GyrA intein subdomain relationships also provided insight into intein evolution...
  22. pmc Protein splicing of the Deinococcus radiodurans strain R1 Snf2 intein
    Maurice W Southworth
    New England Biolabs, Beverly, Massachusetts 01915, USA
    J Bacteriol 184:6387-8. 2002
    ..However, a type strain (ATCC 13939, RF18410) yielded a single protein that splices by the Ala1 protein splicing pathway, with splicing dependent on adjacent residues...
  23. ncbi request reprint Shining a light on protein expression in living organisms
    Francine B Perler
    Nat Chem Biol 3:17-8. 2007
  24. ncbi request reprint The mechanism of intein-mediated protein splicing: variations on a theme
    Kenneth V Mills
    College of the Holy Cross, Department of Chemistry, 1 College Street, Worcester MA 01610, USA
    Protein Pept Lett 12:751-5. 2005
    ..Non-canonical inteins missing conserved nucleophilic residues at the N- or C-terminus likely splice using variations of the standard mechanism...
  25. pmc NMR structure of a KlbA intein precursor from Methanococcus jannaschii
    Margaret A Johnson
    The Scripps Research Institute, Department of Molecular Biology, La Jolla, CA 92037, USA
    Protein Sci 16:1316-28. 2007
    ..Structure-based mutation experiments resulted in a proposal for functional roles of individual residues in the intein catalytic mechanism...
  26. ncbi request reprint The ins and outs of gene expression control
    Francine B Perler
    Nat Biotechnol 22:824-6. 2004