Research Topics
| Richard D MorganSummaryAffiliation: New England Biolabs Country: USA Publications
| Collaborators
|
Detail Information
Publications
Rational engineering of type II restriction endonuclease DNA binding and cleavage specificityRichard D Morgan
New England Biolabs, Inc, Ipswich, MA 01938, USA
Nucleic Acids Res 37:5222-33. 2009..The findings suggest a simple mechanism for the evolution of new DNA specificity in Nature...
The methylomes of six bacteriaIain A Murray
New England Biolabs, 240 County Road, Ipswich, MA 01938, USA
Nucleic Acids Res 40:11450-62. 2012....
Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencingTyson A Clark
Pacific Biosciences, 1380 Willow Road, Menlo Park, CA 94025, USA
Nucleic Acids Res 40:e29. 2012..In addition, we also find evidence of unanticipated MTase promiscuity with some enzymes apparently also modifying sequences that are related, but not identical, to the cognate site...
MmeI: a minimal Type II restriction-modification system that only modifies one DNA strand for host protectionRichard D Morgan
New England Biolabs Inc, Ipswich, MA and MCB Department, Brown University, Providence, RI, USA
Nucleic Acids Res 36:6558-70. 2008..The MmeI architecture represents a minimal approach to assembling a restriction-modification system wherein a single DNA recognition domain targets both the endonuclease and DNA methyltransferase activities...
The MmeI family: type II restriction-modification enzymes that employ single-strand modification for host protectionRichard D Morgan
New England Biolabs, Inc, Ipswich, MA 01938, USA
Nucleic Acids Res 37:5208-21. 2009....
A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genesRichard J Roberts
New England Biolabs, Beverly, MA 01915, USA
Nucleic Acids Res 31:1805-12. 2003..It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes...
Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variantsJames C Samuelson
New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
Nucleic Acids Res 34:796-805. 2006..2 x 10(4) U/mg, a 32-fold increase over variant E156K. A comprehensive analysis indicates that the cleavage specificity of M91V/E156K is relaxed to a small set of 8 bp substrates while retaining activity towards the NotI sequence...
Using shotgun sequence data to find active restriction enzyme genesYu Zheng
New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
Nucleic Acids Res 37:e1. 2009..Active restriction enzyme genes have been identified, and their activity demonstrated biochemically, in the sequenced genomes of Methanocaldococcus jannaschii, Bacillus cereus ATCC 10987 and Methylococcus capsulatus...
