Research Topics
Genomes and Genes
| Narla MohandasSummaryAffiliation: New York Blood Center Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
New insights into function of red cell membrane proteins and their interaction with spectrin-based membrane skeletonN Mohandas
Red Cell Physiology Laboratory, Lindsley F. Kimball Research Institute, New York Blood Center, 310 East 67(th) Street, New York, NY 10021, USA
Transfus Clin Biol 13:29-30. 2006- Blood group antigens in health and diseaseNarla Mohandas
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10021, USA
Curr Opin Hematol 12:135-40. 2005..They have long played an important role in identifying matched blood products for transfusion. Recent studies have identified varied and important functions for some of these molecules in cell physiology and human pathology... 
Malaria and human red blood cellsNarla Mohandas
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10065, USA
Med Microbiol Immunol 201:593-8. 2012....
Red cell membrane: past, present, and futureNarla Mohandas
Red Cell Physiology Laboratory, New York Blood Center, New York, NY, USA
Blood 112:3939-48. 2008....- Hereditary spherocytosis and hereditary elliptocytosis: aberrant protein sorting during erythroblast enucleationMarcela Salomao
The Red Cell Physiology Laboratory, The New York Blood Center, New York, NY, USA
Blood 116:267-9. 2010..We conclude that aberrant protein sorting is one mechanistic basis for protein deficiencies in HE and HS... - Protein 4.1R-dependent multiprotein complex: new insights into the structural organization of the red blood cell membraneMarcela Salomao
Red Cell Physiology Laboratory and Membrane Biochemistry Laboratory, New York Blood Center, New York, NY 10065, USA
Proc Natl Acad Sci U S A 105:8026-31. 2008.... - Plasmodium falciparum erythrocyte membrane protein 3 (PfEMP3) destabilizes erythrocyte membrane skeletonXinhong Pei
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10065, USA
J Biol Chem 282:26754-8. 2007..We conjecture that the loss of mechanical cohesion of the membrane may facilitate the exit of the mature merozoites from the cell... - Comprehensive characterization of expression patterns of protein 4.1 family members in mouse adrenal gland: implications for functionsHua Wang
Red Cell Physiology Laboratory, New York Blood Center, 310 East 67th St, New York, NY 10065, USA
Histochem Cell Biol 134:411-20. 2010..The characterization of distinct splice forms of various 4.1 proteins with diverse cellular and sub-cellular localization indicates multiple functions for this family of proteins in endocrine functions of adrenal gland... - Lack of protein 4.1G causes altered expression and localization of the cell adhesion molecule nectin-like 4 in testis and can cause male infertilityShaomin Yang
Red Cell Physiology Laboratory, New York Blood Center, New York, New York 10065, USA
Mol Cell Biol 31:2276-86. 2011..Additionally, the finding that infertility is present in B6-129 but not on the B6 background suggests the presence of a major modifier gene(s) that influences 4.1G function and is associated with male infertility... - Membrane remodeling during reticulocyte maturationJing Liu
Red Cell Physiology Laboratory, New York Blood Center, New York City, NY 10065, USA
Blood 115:2021-7. 2010..1R in reticulocytes, which leads to a decrease in shear resistance by reducing its interaction with spectrin and actin. These observations begin to unravel the mechanistic basis of crucial changes accompanying reticulocyte maturation... - The ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum stabilizes spectrin tetramers and suppresses further invasionXinhong Pei
Red Cell Physiology Laboratory, New York Blood Center, 310 E 67th Street, New York, NY 10021, USA
Blood 110:1036-42. 2007.... - Phosphatidylinositol-4,5-biphosphate (PIP2) differentially regulates the interaction of human erythrocyte protein 4.1 (4.1R) with membrane proteinsXiuli An
Red Cell Physiology Laboratory, New York Blood Center, 310 East 67th Street, New York, New York 10021, USA
Biochemistry 45:5725-32. 2006..1R, 4.1B, 4.1G, and 4.1N) are widely expressed and the PIP2-binding motifs are highly conserved, it is likely that the functions of other 4.1 proteins are similarly regulated by PIP2 in many different cell types... - Cytoskeletal protein 4.1R negatively regulates T-cell activation by inhibiting the phosphorylation of LATQiaozhen Kang
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10065, USA
Blood 113:6128-37. 2009..1R display an elevated humoral response to immunization with T cell-dependent antigen. Thus, we have defined a hitherto unrecognized role for 4.1R in negatively regulating T-cell activation by modulating intracellular signal transduction... - Control of erythrocyte membrane-skeletal cohesion by the spectrin-membrane linkageLionel Blanc
Red Cell Physiology Laboratory, New York Blood Center, New York, New York 10065, USA
Biochemistry 49:4516-23. 2010..These findings enabled us to identify an additional important functional role for the spectrin-ankyrin-band 3 link in regulating spectrin self-association in the red cell membrane... - Identification and functional characterization of protein 4.1R and actin-binding sites in erythrocyte beta spectrin: regulation of the interactions by phosphatidylinositol-4,5-bisphosphateXiuli An
Red Cell Physiology Laboratory, New York Blood Center, New York, New York 10021, USA
Biochemistry 44:10681-8. 2005..1R ternary complex in vitro. Furthermore, the binding of 4.1R to 1-301 is greatly enhanced by PIP(2), implying the existence of a regulatory switch in the cell... - Conformational stabilities of the structural repeats of erythroid spectrin and their functional implicationsXiuli An
Red Cell Physiology Laboratory, New York Blood Center, 310 E 67th Street, New York, NY 10021, USA
J Biol Chem 281:10527-32. 2006.... - Tropomyosin modulates erythrocyte membrane stabilityXiuli An
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10021, USA
Blood 109:1284-8. 2007..These findings have enabled us identify a function for TM in elevating the mechanical stability of erythrocyte membranes by stabilizing the spectrin-actin-4.1R junctional complex... - Thermal stabilities of brain spectrin and the constituent repeats of subunitsXiuli An
Red Cell Physiology Laboratory, New York Blood Center, New York, New York 10021, USA
Biochemistry 45:13670-6. 2006..The greater structural stability of the repeats in alphaII- and betaII-spectrin may account, at least in part, for the higher rigidity of brain compared to erythrocyte spectrin... - Phospholipid binding by proteins of the spectrin family: a comparative studyXiuli An
Red Cell Physiology Laboratory, Lindsley F Kimball Research Institute, New York Blood Center, New York, NY 10021, USA
Biochem Biophys Res Commun 327:794-800. 2005.... - Protein 4.1R regulates cell adhesion, spreading, migration and motility of mouse keratinocytes by modulating surface expression of beta1 integrinLixiang Chen
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10065, USA
J Cell Sci 124:2478-87. 2011..These data enabled the identification of a functional role for protein 4.1R in keratinocytes by modulating the surface expression of β1 integrin, possibly through a direct association between 4.1R and β1 integrin... - Adhesive activity of Lu glycoproteins is regulated by interaction with spectrinXiuli An
Red Cell Physiology Laboratory, New York Blood Center, NY, USA
Blood 112:5212-8. 2008..Importantly, disruption of the Lu-spectrin linkage was accompanied by enhanced cell adhesion to laminin. We conclude that the interaction of the Lu cytoplasmic tail with the cytoskeleton regulates its adhesive receptor function... - Mammalian alpha I-spectrin is a neofunctionalized polypeptide adapted to small highly deformable erythrocytesMarcela Salomao
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10021, USA
Proc Natl Acad Sci U S A 103:643-8. 2006..We conclude that alphaI-spectrin represents a neofunctionalized spectrin adapted to the rapid make and break of tetramers... - Quantitative analysis of murine terminal erythroid differentiation in vivo: novel method to study normal and disordered erythropoiesisJing Liu
Red Cell Physiology Laboratory, New York Blood Center, New York, NY, USA
Blood 121:e43-9. 2013..The means to quantitate in vivo murine erythropoiesis using our approach will probably have broad application in the study of altered erythropoiesis in various red cell disorders... - The erythroid niche: molecular processes occurring within erythroblastic islandsNarla Mohandas
The Red Cell Physiology Laboratory, The New York Blood Center, 310 East 67th Street, New York, NY 10065, USA
Transfus Clin Biol 17:110-1. 2010..Hence, it appears that abnormal protein sorting generates specific protein deficiencies in hereditary elliptocytosis and hereditary spherocytosis... - Inter-subunit interactions in erythroid and non-erythroid spectrinsXiuli An
Lindsley F Kimball Research Institute, New York Blood Center, New York, NY 10065, USA
Biochim Biophys Acta 1814:420-7. 2011.... - Phosphatidylserine binding sites in red cell spectrinXiuli An
Red Cell Physiology Laboratory, Lindsley F Kimball Research Institute, New York Blood Center, 310 East 67th Street, New York, NY 10021, USA
Blood Cells Mol Dis 32:430-2. 2004..1R for PS) the formation of PS-rich lipid domains, which have been observed in the red cell membrane, may be a result... - Structural and functional studies of interaction between Plasmodium falciparum knob-associated histidine-rich protein (KAHRP) and erythrocyte spectrinXinhong Pei
Red Cell Physiology Laboratory, New York Blood Center, New York, New York, 10021, USA
J Biol Chem 280:31166-71. 2005..As the presence of KAHRP at the erythrocyte membrane is necessary for cytoadherence in vivo, our findings have implications for the development of new therapies for mitigating the severity of malaria infection... - A Golgi-associated protein 4.1B variant is required for assimilation of proteins in the membraneQiaozhen Kang
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10065, USA
J Cell Sci 122:1091-9. 2009..Thus, this newly identified Golgi-specific protein 4.1 appears to have an essential role in maintaining the structure of the Golgi and in assembly of a subset of membrane proteins... - Protein 4.1R links E-cadherin/beta-catenin complex to the cytoskeleton through its direct interaction with beta-catenin and modulates adherens junction integrityShaomin Yang
Red Cell Physiology Laboratory, New York Blood Center, 310 East 67th St, New York, NY 10065, USA
Biochim Biophys Acta 1788:1458-65. 2009..1R in linking the cadherin/catenin complex to the cytoskeleton through its direct interaction with beta-catenin and in regulating the integrity of adherens junction... - Phosphorylation-dependent perturbations of the 4.1R-associated multiprotein complex of the erythrocyte membraneEmilie Gauthier
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10065, USA
Biochemistry 50:4561-7. 2011..1R results in structural changes transmitted to the functional interaction centers of the protein. We consider possible implications of our findings for the altered membrane function of normal reticulocytes and sickle red cells... - Phosphatidylserine binding sites in erythroid spectrin: location and implications for membrane stabilityXiuli An
Red Cell Physiology Laboratory, Lindsley F Kimball Research Institute, New York Blood Center, New York, New York 10021, USA
Biochemistry 43:310-5. 2004..1R for PS) the formation of PS-rich lipid domains, which have been observed in the red cell membrane, may be a result... - Impaired Intestinal Calcium Absorption in Protein 4.1R-deficient Mice Due to Altered Expression of Plasma Membrane Calcium ATPase 1b (PMCA1b)Congrong Liu
From the Red Cell Physiology Laboratory, New York Blood Center, New York, New York 10065
J Biol Chem 288:11407-15. 2013..1R and the second intracellular loop and C terminus of PMCA1b. Our findings have enabled us to define a functional role for 4.1R in small intestinal calcium absorption through regulation of membrane expression of PMCA1b... - Resolving the distinct stages in erythroid differentiation based on dynamic changes in membrane protein expression during erythropoiesisKe Chen
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10065, USA
Proc Natl Acad Sci U S A 106:17413-8. 2009.... - Membrane assembly during erythropoiesisJing Liu
Laboratory of Red Cell Physiology, New York Blood Center, New York, New York, USA
Curr Opin Hematol 18:133-8. 2011.... - The water channel aquaporin-1 partitions into exosomes during reticulocyte maturation: implication for the regulation of cell volumeLionel Blanc
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10065, USA
Blood 114:3928-34. 2009..These results lead us to suggest that AQP-1 sorting into exosomes may be the mechanism by which the reticulocyte adapts to environmental changes during its maturation... - Native ultrastructure of the red cell cytoskeleton by cryo-electron tomographyANDREA NANS
Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, New York, USA
Biophys J 101:2341-50. 2011..Based on comparisons with expanded skeletons, we propose that the oligomeric state of spectrin is in a dynamic equilibrium that facilitates remodeling of the network as the cell changes shape in response to shear stress... - Erythroblastic islands, terminal erythroid differentiation and reticulocyte maturationXiuli An
Laboratory of Membrane Biology, The New York Blood Center, New York, NY, USA
Int J Hematol 93:139-43. 2011..This review summarizes our current understanding of the role of erythroblastic islands in erythropoiesis, membrane assembly during terminal erythroid differentiation and cellular and membrane reorganization during reticulocyte maturation... - Isolation and functional characterization of human erythroblasts at distinct stages: implications for understanding of normal and disordered erythropoiesis in vivoJingping Hu
Laboratory of Membrane Biology, New York Blood Center, New York, NY
Blood 121:3246-53. 2013.... - Shear-response of the spectrin dimer-tetramer equilibrium in the red blood cell membraneXiuli An
Red Cell Physiology Laboratory, The New York Blood Center, New York, New York 10021, USA
J Biol Chem 277:31796-800. 2002.... - Morphological and functional platelet abnormalities in Berkeley sickle cell miceArun S Shet
The Laboratory of Blood and Vascular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
Blood Cells Mol Dis 41:109-18. 2008..Thus, additional studies are needed to assess whether large platelets contribute either to pulmonary hypertension or the large vessel arterial occlusion that produces stroke in some children with sickle cell disease... - Congenital erythropoietic porphyria: characterization of murine models of the severe common (C73R/C73R) and later-onset genotypesDavid F Bishop
Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, New York, USA
Mol Med 17:748-56. 2011..Of significance for therapeutic intervention, these mouse models suggest that only 11% of wild-type activity might be needed to reverse the pathology in CEP patients... - Disorders of red cell membraneXiuli An
Red Cell Physiology Laboratory, New York Blood Center, New York, NY 10065, USA
Br J Haematol 141:367-75. 2008..Importantly, the severity of anaemia in both these disorders is directly related to extent of membrane surface area loss. Splenectomy results in amelioration of anaemia... - The 4.1B cytoskeletal protein regulates the domain organization and sheath thickness of myelinated axonsSteven Einheber
School of Health Sciences, Hunter College, City University of New York, New York, New York, USA
Glia 61:240-53. 2013..1B. These results demonstrate that 4.1B is a key cytoskeletal scaffold for axonal adhesion molecules expressed in the juxtaparanodal and internodal domains that unexpectedly regulates myelin sheath thickness... - Red blood cell blood group antigens: structure and functionMarion E Reid
Laboratology of Immunology and the Lindsley F. Kimball Research Institute, New York Blood Center, 310 E. 67th Street, New York, NY 10021, USA
Semin Hematol 41:93-117. 2004.... - Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domainJianbin Peng
Biochemistry and Molecular Genetics Laboratory, Lindsley F Kimball Research Institute, New York Blood Center, 310 East, 67th St, New York, NY 10021, USA
Biochim Biophys Acta 1759:562-72. 2006..Taken together, Dusty is a unique evolutionarily selected group of divergent protein kinases that may play important functional roles in the brain and other tissues of vertebrates... - Primary role for adherent leukocytes in sickle cell vascular occlusion: a new paradigmAslihan Turhan
Department of Medicine, Mount Sinai School of Medicine, 1 Gustave Levy Place, Box 1079, New York, NY 10029, USA
Proc Natl Acad Sci U S A 99:3047-51. 2002.... - Hepcidin as a therapeutic tool to limit iron overload and improve anemia in β-thalassemic miceSara Gardenghi
Weill Cornell Medical College, New York, New York, USA
J Clin Invest 120:4466-77. 2010..These data led us to suggest that therapeutics that could increase hepcidin levels or act as hepcidin agonists might help treat the abnormal iron absorption in individuals with β-thalassemia and related disorders... - Ribosomal protein S19 expression during erythroid differentiationLydie Da Costa
New York Blood Center, NY 10021, USA
Blood 101:318-24. 2003..We anticipate that these findings will contribute to further development of our understanding of the contribution of RPS19 to erythropoiesis...
Research Grants
- PROGRAM PROJECT: RED CELL MEMBRANE STUDIESMOHANDAS NARLA; Fiscal Year: 2006..abstract_text> ..
- Diamond-Blackfan Anemia and Ribosomal Protein S19MOHANDAS NARLA; Fiscal Year: 2007..Furthermore, it is our hope that the new insights generated by our studies might lead to the development of new therapeutic strategies for the treatment of DBA patients presenting with RPS19 gene mutations. ..
- RHEOLOGICAL AND ADHERENCE PROPERTIES OF SICKLE CELLSMOHANDAS NARLA; Fiscal Year: 2010..We anticipate that our findings will lead to the identification of useful therapeutic strategies for the effective clinical management of this debilitating human disease. ..
- RHEOLOGICAL AND ADHERENCE PROPERTIES OF SICKLE CELLSMOHANDAS NARLA; Fiscal Year: 2005..Our application of these refined systems offers great promise for elucidating the cause of painful vaso-occlusion and identifying potential targets for future therapy. ..
- RED CELL DEFORMABILITY IN VITRO AND SURVIVAL IN VIVOMOHANDAS NARLA; Fiscal Year: 2007..abstract_text> ..
- RHEOLOGICAL AND ADHERENCE PROPERTIES OF SICKLE CELLSMOHANDAS NARLA; Fiscal Year: 2007..We anticipate that our findings will lead to the identification of useful therapeutic strategies for the effective clinical management of this debilitating human disease. ..
- RHEOLOGICAL AND ADHERENCE PROPERTIES OF SICKLE CELLSMOHANDAS NARLA; Fiscal Year: 1991....
- RHEOLOGICAL AND ADHERENCE PROPERTIES OF SICKLE CELLSMOHANDAS NARLA; Fiscal Year: 1993..This in turn, should enable the development and critical testing of hypotheses concerning the contributions of various cellular abnormalities to the pathophysiology of sickle cell disease...
- RHEOLOGICAL AND ADHERENCE PROPERTIES OF SICKLE CELLSMOHANDAS NARLA; Fiscal Year: 2000..The observed heterogeneity in sickle cell adhesion is due to heterogeneity of expression of specific adhesive receptor(s) on distinct sub populations of sickle cells. ..