Jeffrey Kaplan

Summary

Affiliation: New Jersey
Country: USA

Publications

  1. pmc Poly-N-acetylglucosamine mediates biofilm formation and detergent resistance in Aggregatibacter actinomycetemcomitans
    Era A Izano
    Department of Oral Biology, New Jersey Dental School, 185 S Orange Avenue, Newark, NJ 07103, USA
    Microb Pathog 44:52-60. 2008
  2. ncbi Therapeutic potential of biofilm-dispersing enzymes
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    Int J Artif Organs 32:545-54. 2009
  3. pmc Low levels of β-lactam antibiotics induce extracellular DNA release and biofilm formation in Staphylococcus aureus
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ, USA
    MBio 3:e00198-12. 2012
  4. pmc Recombinant human DNase I decreases biofilm and increases antimicrobial susceptibility in staphylococci
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    J Antibiot (Tokyo) 65:73-7. 2012
  5. pmc Extracellular DNA-dependent biofilm formation by Staphylococcus epidermidis RP62A in response to subminimal inhibitory concentrations of antibiotics
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Medical Science Building, Room C636, 185 S Orange Ave, Newark, NJ 07103, USA
    Res Microbiol 162:535-41. 2011
  6. pmc Biofilm dispersal: mechanisms, clinical implications, and potential therapeutic uses
    J B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    J Dent Res 89:205-18. 2010
  7. pmc Structural and genetic analyses of O polysaccharide from Actinobacillus actinomycetemcomitans serotype f
    J B Kaplan
    Department of Oral Pathology, Biology and Diagnostic Sciences, New Jersey Dental School, Newark, New Jersey 07103 2714, USA
    Infect Immun 69:5375-84. 2001
  8. ncbi Biofilm formation is prevalent among field isolates of Actinobacillus pleuropneumoniae
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    Vet Microbiol 108:89-94. 2005
  9. pmc Genes involved in the synthesis and degradation of matrix polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae biofilms
    Jeffrey B Kaplan
    Medical Science Building, Room C 636, 185 S Orange Ave, Newark, NJ 07103, USA
    J Bacteriol 186:8213-20. 2004
  10. pmc Enzymatic detachment of Staphylococcus epidermidis biofilms
    Jeffrey B Kaplan
    Medical Science Building, Room C 636, 185 S Orange Avenue, Newark NJ 07103, USA
    Antimicrob Agents Chemother 48:2633-6. 2004

Research Grants

  1. Biofilm growth and detachment of an oral pathogen
    Jeffrey Kaplan; Fiscal Year: 2007

Collaborators

Detail Information

Publications33

  1. pmc Poly-N-acetylglucosamine mediates biofilm formation and detergent resistance in Aggregatibacter actinomycetemcomitans
    Era A Izano
    Department of Oral Biology, New Jersey Dental School, 185 S Orange Avenue, Newark, NJ 07103, USA
    Microb Pathog 44:52-60. 2008
    ..Our findings suggest that PGA, extracellular DNA and proteinaceous adhesins all contribute to the structural integrity of the A. actinomycetemcomitans biofilm matrix...
  2. ncbi Therapeutic potential of biofilm-dispersing enzymes
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    Int J Artif Organs 32:545-54. 2009
    ..In vitro and in vivo studies demonstrating the anti-biofilm activities of these two enzymes will be summarized, and the therapeutic potential and possible drawbacks of using these enzymes as clinical agents will be discussed...
  3. pmc Low levels of β-lactam antibiotics induce extracellular DNA release and biofilm formation in Staphylococcus aureus
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ, USA
    MBio 3:e00198-12. 2012
    ..Our findings demonstrate that subminimal inhibitory concentrations of β-lactam antibiotics significantly induce autolysin-dependent extracellular DNA release and biofilm formation in some strains of S. aureus...
  4. pmc Recombinant human DNase I decreases biofilm and increases antimicrobial susceptibility in staphylococci
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    J Antibiot (Tokyo) 65:73-7. 2012
    ..aureus and S. epidermidis at clinically achievable concentrations. rhDNase, either alone or in combination with antimicrobial agents, may have applications in treating or preventing staphylococcal biofilm-related infections...
  5. pmc Extracellular DNA-dependent biofilm formation by Staphylococcus epidermidis RP62A in response to subminimal inhibitory concentrations of antibiotics
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Medical Science Building, Room C636, 185 S Orange Ave, Newark, NJ 07103, USA
    Res Microbiol 162:535-41. 2011
    ..epidermidis is both strain- and antibiotic-dependent and that S. epidermidis RP62A utilizes an extracellular DNA-dependent mechanism to form biofilms in response to sub-MIC antibiotics...
  6. pmc Biofilm dispersal: mechanisms, clinical implications, and potential therapeutic uses
    J B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    J Dent Res 89:205-18. 2010
    ..The clinical implications of biofilm dispersal and the potential therapeutic applications of some of the most recent findings will also be discussed...
  7. pmc Structural and genetic analyses of O polysaccharide from Actinobacillus actinomycetemcomitans serotype f
    J B Kaplan
    Department of Oral Pathology, Biology and Diagnostic Sciences, New Jersey Dental School, Newark, New Jersey 07103 2714, USA
    Infect Immun 69:5375-84. 2001
    ..The finding of an A. actinomycetemcomitans serotype showing serological cross-reactivity with anti-serotype b-specific antiserum suggests that a reevaluation of strains previously classified as serotype b may be warranted...
  8. ncbi Biofilm formation is prevalent among field isolates of Actinobacillus pleuropneumoniae
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    Vet Microbiol 108:89-94. 2005
    ..Biofilm formation may have relevance to the colonization, pathogenesis and transmission of this bacterium...
  9. pmc Genes involved in the synthesis and degradation of matrix polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae biofilms
    Jeffrey B Kaplan
    Medical Science Building, Room C 636, 185 S Orange Ave, Newark, NJ 07103, USA
    J Bacteriol 186:8213-20. 2004
    ..actinomycetemcomitans and A. pleuropneumoniae biofilms...
  10. pmc Enzymatic detachment of Staphylococcus epidermidis biofilms
    Jeffrey B Kaplan
    Medical Science Building, Room C 636, 185 S Orange Avenue, Newark NJ 07103, USA
    Antimicrob Agents Chemother 48:2633-6. 2004
    ..Precoating surfaces with the enzyme prevented S. epidermidis biofilm formation. Our findings demonstrate that biofilm-releasing enzymes can exhibit broad-spectrum activity and that these enzymes may be useful as antibiofilm agents...
  11. pmc Biofilm dispersal of Neisseria subflava and other phylogenetically diverse oral bacteria
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, New Jersey 07103, USA
    Appl Environ Microbiol 68:4943-50. 2002
    ..This in vitro spreading phenotype could be a useful tool for studying biofilm dispersal in these and other nonflagellated bacteria and may have physiological relevance to biofilm dispersal in the oral cavity...
  12. pmc Biofilm growth and detachment of Actinobacillus actinomycetemcomitans
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School Electron Microscopy Facility, New Jersey Medical School, Newark, New Jersey 07103, USA
    J Bacteriol 185:1399-404. 2003
    ..actinomycetemcomitans biofilm growth and detachment are discrete processes and that biofilm cell detachment evidently involves the formation of nonaggregated cells inside the biofilm colony that are destined for release from the colony...
  13. pmc Detachment of Actinobacillus actinomycetemcomitans biofilm cells by an endogenous beta-hexosaminidase activity
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, New Jersey 07103, USA
    J Bacteriol 185:4693-8. 2003
    ..We concluded that dspB encodes a soluble beta-N-acetylglucosaminidase that causes detachment and dispersion of A. actinomycetemcomitans biofilm cells...
  14. doi Antibiotic-induced biofilm formation
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    Int J Artif Organs 34:737-51. 2011
    ..It is still unclear whether antibiotic-induced biofilm formation contributes to the inconsistent success of antimicrobial therapy for device infections...
  15. doi Modeling and biochemical analysis of the activity of antibiofilm agent Dispersin B
    J E Kerrigan
    Academic Systems and Technologies, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    Acta Biol Hung 59:439-51. 2008
    ..Our combined results indicate that DspB is an exo-acting enzyme that hydrolyzes beta(1,6)-linked N-acetylglucosamine oligomers...
  16. ncbi Sequence diversity in the major fimbrial subunit gene (flp-1) of Actinobacillus actinomycetemcomitans
    J B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, NJ, USA
    Oral Microbiol Immunol 17:354-9. 2002
    ..Our findings provide a framework for future biochemical, immunological and genetic studies of A. actinomycetemcomitans fimbriae...
  17. ncbi Detachment and killing of Aggregatibacter actinomycetemcomitans biofilms by dispersin B and SDS
    E A Izano
    Department of Oral Biology, New Jersey Dental School, Medical Science Building, Newark, NJ 07103, USA
    J Dent Res 86:618-22. 2007
    ..actinomycetemcomitans cells from detachment and killing by SDS. Dispersin B and SDS may be useful agents for treating chronic infections caused by A. actinomycetemcomitans and other PGA-producing bacteria...
  18. ncbi Structural analysis of dispersin B, a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans
    N Ramasubbu
    Department of Oral Biology, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    J Mol Biol 349:475-86. 2005
    ....
  19. doi Aggregatibacter actinomycetemcomitans serotype f O-polysaccharide mediates coaggregation with Fusobacterium nucleatum
    D Rupani
    Department of Oral Biology, New Jersey Dental School, Newark, NJ, USA
    Oral Microbiol Immunol 23:127-30. 2008
    ..Intergeneric bacterial coaggregation may play an important role in plaque development...
  20. pmc Broad-spectrum biofilm inhibition by Kingella kingae exopolysaccharide
    Meriem Bendaoud
    Department of Oral Biology, New Jersey Dental School, Newark, New Jersey 07103, USA
    J Bacteriol 193:3879-86. 2011
    ..PAM galactan is one of a growing number of bacterial polysaccharides that exhibit antibiofilm activity. The biological roles and potential technological applications of these molecules remain unknown...
  21. pmc Population structure and genetic diversity of Actinobacillus actinomycetemcomitans strains isolated from localized juvenile periodontitis patients
    Jeffrey B Kaplan
    Department of Oral Biology, New Jersey Dental School, Newark, New Jersey 07103 2714, USA
    J Clin Microbiol 40:1181-7. 2002
    ..Strains from all three major lineages were recovered from LJP patients, suggesting that phylogenetically diverse strains of A. actinomycetemcomitans carry pathogenic potential...
  22. pmc Both leukotoxin and poly-N-acetylglucosamine surface polysaccharide protect Aggregatibacter actinomycetemcomitans cells from macrophage killing
    Vishwanath Venketaraman
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    Microb Pathog 45:173-80. 2008
    ..We concluded that both leukotoxin and PGA are necessary for evasion of macrophage killing by A. actinomycetemcomitans...
  23. pmc Screen for leukotoxin mutants in Aggregatibacter actinomycetemcomitans: genes of the phosphotransferase system are required for leukotoxin biosynthesis
    Maria P Isaza
    Department of Oral Biology, University of Medicine and Dentistry of New Jersey, 185 S Orange Avenue, Medical Science Building C 636, Newark, NJ 07103, USA
    Infect Immun 76:3561-8. 2008
    ..The levels of cAMP in the ptsH mutant were significantly lower than in the wild-type strain, and LtxA production could be restored by adding exogenous cAMP to the culture...
  24. doi Microfluidic devices for studying growth and detachment of Staphylococcus epidermidis biofilms
    Joung Hyun Lee
    New Jersey Center for MicroChemical Systems, Department of Chemical, Biomedical and Materials Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA
    Biomed Microdevices 10:489-98. 2008
    ..Combined dynamic delivery of dispersin B and rifampicin was found to be effective for complete removal of the S. epidermidis biofilm...
  25. pmc The Actinobacillus actinomycetemcomitans autotransporter adhesin Aae exhibits specificity for buccal epithelial cells from humans and old world primates
    Daniel H Fine
    Medical Science Building, Room C 636, 185 S Orange Ave, Newark, NJ 07103, USA
    Infect Immun 73:1947-53. 2005
    ..Our findings indicate that Aae mediates binding of A. actinomycetemcomitans to BECs from humans and Old World primates and that this process may contribute to the host range specificity and tissue tropism exhibited by this bacterium...
  26. ncbi How we got attached to Actinobacillus actinomycetemcomitans: A model for infectious diseases
    Daniel H Fine
    Center for Oral Infectious Diseases, Department of Oral Biology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA
    Periodontol 2000 42:114-57. 2006
  27. ncbi Polysaccharide intercellular adhesin or protein factors in biofilm accumulation of Staphylococcus epidermidis and Staphylococcus aureus isolated from prosthetic hip and knee joint infections
    Holger Rohde
    Institut fur Medizinische Mikrobiologie, Virologie und Hygiene, Universitatsklinikum Hamburg Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany
    Biomaterials 28:1711-20. 2007
    ..In S. aureus biofilm formation proteins are of overall importance and future work should focus on the identification of functionally active molecules...
  28. pmc Poly-N-acetylglucosamine mediates biofilm formation and antibiotic resistance in Actinobacillus pleuropneumoniae
    Era A Izano
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    Microb Pathog 43:1-9. 2007
    ..Our findings suggest that PGA functions as a major biofilm adhesin in A. pleuropneumoniae. Biofilm formation may have relevance to the colonization and pathogenesis of A. pleuropneumoniae in pigs...
  29. pmc Tight-adherence genes of Actinobacillus actinomycetemcomitans are required for virulence in a rat model
    Helen C Schreiner
    Department of Oral Biology, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    Proc Natl Acad Sci U S A 100:7295-300. 2003
    ..These results demonstrate the critical importance of the tad locus in the colonization and pathogenesis of A. actinomycetemcomitans...
  30. pmc A second Aggregatibacter actinomycetemcomitans autotransporter adhesin exhibits specificity for buccal epithelial cells in humans and Old World primates
    Gang Yue
    Department of Oral Biology, New Jersey Dental School, Newark, NJ 07103, USA
    Infect Immun 75:4440-8. 2007
    ..actinomycetemcomitans to human BECs. Since the BEC is a prominent reservoir for A. actinomycetemcomitans, identification of this second adhesin could lead to important therapeutic strategies...
  31. ncbi Role of active-site residues of dispersin B, a biofilm-releasing beta-hexosaminidase from a periodontal pathogen, in substrate hydrolysis
    Suba G A Manuel
    Department of Oral Biology, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA
    FEBS J 274:5987-99. 2007
    ....
  32. pmc Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms
    Era A Izano
    Medical Science Building, Room C 636, 185 S Orange Ave, Newark, NJ 07103, USA
    Appl Environ Microbiol 74:470-6. 2008
    ..epidermidis biofilms to CPC killing, whereas DNase I sensitized S. aureus biofilms to CPC killing. We concluded that PNAG and ecDNA play fundamentally different structural roles in S. aureus and S. epidermidis biofilms...
  33. pmc One of two human lactoferrin variants exhibits increased antibacterial and transcriptional activation activities and is associated with localized juvenile periodontitis
    Kabilan Velliyagounder
    Department of Oral Biology, New Jersey Dental School, Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    Infect Immun 71:6141-7. 2003
    ..Our findings suggest that these two lactoferrin variants are functionally different and that these differences may contribute to the pathogenesis of localized juvenile periodontitis...

Research Grants4

  1. Biofilm growth and detachment of an oral pathogen
    Jeffrey Kaplan; Fiscal Year: 2007
    ..abstract_text> ..