Research Topics
Genomes and Genes
| A L JoynerSummaryAffiliation: New York University Country: USA Publications
Research Grants
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Detail Information
Publications
Two lineage boundaries coordinate vertebrate apical ectodermal ridge formationR A Kimmel
Department of Cell Biology, New York University School of Medicine, New York, New York 10016 USA
Genes Dev 14:1377-89. 2000..Finally, fate mapping of AER domains in these mutants showed that En1 plays a part in positioning and maintaining the two lineage borders...
Mouse Gli1 mutants are viable but have defects in SHH signaling in combination with a Gli2 mutationH L Park
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology and Physiology and Neuroscience, New York University Medical School, New York, NY 10016, USA
Development 127:1593-605. 2000..Furthermore, Gli1 and Gli2, but not Gli1 and Gli3, have extensive overlapping functions that are likely downstream of SHH signaling...- Genetic inducible fate mapping in mouse: establishing genetic lineages and defining genetic neuroanatomy in the nervous systemAlexandra L Joyner
Howard Hughes Medical Institute and Developmental Genetics Program, Department of Cell Biology and Physiology and Neuroscience, New York University School of Medicine, New York, New York 10016, USA
Dev Dyn 235:2376-85. 2006..We end with a look toward the future at a powerful new approach that combines genetic fate mapping with cellular phenotyping alleles to study cell morphology, physiology, and function using examples from the nervous system... - Otx2, Gbx2 and Fgf8 interact to position and maintain a mid-hindbrain organizerA L Joyner
Department of Cell Biology, NYU School of Medicine, 540 First Avenue, New York, New York 10016, USA
Curr Opin Cell Biol 12:736-41. 2000..Recent experiments have shown that Otx2, Gbx2 and Fgf8 genes play a major role in the positioning and functioning of this organizing center... - FGF8 can activate Gbx2 and transform regions of the rostral mouse brain into a hindbrain fateA Liu
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
Development 126:4827-38. 1999.... - EN and GBX2 play essential roles downstream of FGF8 in patterning the mouse mid/hindbrain regionA Liu
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
Development 128:181-91. 2001.... - Gli1 can rescue the in vivo function of Gli2C B Bai
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Development 128:5161-72. 2001..We show that the defects are enhanced when Gli3 function is reduced, demonstrating that an important difference between Gli1 and Gli2 is the ability of Gli1 to antagonize Gli3 function... - Gli2 is required for induction of floor plate and adjacent cells, but not most ventral neurons in the mouse central nervous systemM P Matise
Developmental Genetics Program and Howard Hughes Medical Institute, and Department of Cell Biology and Physiology and Neuroscience, NYU Medical Center, New York, NY 10016, USA
Development 125:2759-70. 1998.... - Otx2 and Gbx2 are required for refinement and not induction of mid-hindbrain gene expressionJ Y Li
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Development 128:4979-91. 2001..Furthermore, Otx2 and Gbx2 are required to suppress hindbrain and midbrain development, respectively, and thus allow establishment of the normal spatial domains of Fgf8 and other genes... - Alteration of limb and brain patterning in early mouse embryos by ultrasound-guided injection of Shh-expressing cellsA Liu
Skirball Institute of Biomolecular Medicine, New York University Medical Center, 540 First Avenue, New York, NY 10016, USA
Mech Dev 75:107-15. 1998..In combination with the many available mouse mutants, this method offers a new approach for analyzing genetic interactions through gain-of-function studies performed in mutant mouse backgrounds... - A role for Gbx2 in repression of Otx2 and positioning the mid/hindbrain organizerS Millet
Developmental Genetics Program and Howard Hughes Medical Institute, Skirball Institute of Biomolecular Medicine, New York, New York 10016, USA
Nature 401:161-4. 1999..5-10. We propose that formation of a normal MHB organizer depends on a sharp Otx2 caudal border and that Gbx2 is required to position and sharpen this border... - Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbsC A Loomis
Ronald O Perelman Department of Dermatology, NYU Medical School, New York, NY 10016, USA
Development 125:1137-48. 1998..This leads to induction of a second zone of compaction ventrally, which in some cases goes on to form an autonomous secondary AER... - Regionalization of Sonic hedgehog transcription along the anteroposterior axis of the mouse central nervous system is regulated by Hnf3-dependent and -independent mechanismsD J Epstein
Developmental Genetics Program and Howard Hughes Medical Institute, Skirball Institute of Biomolecular Medicine, and Department of Cellular, Molecular and Developmental Biology, Harvard University, Cambridge MA, USA
Development 126:281-92. 1999..Taken together, our results support the existence of Hnf3-dependent and -independent mechanisms in the direct activation of Shh transcription within the CNS and axial mesoderm... - Ventral midline cells are required for the local control of commissural axon guidance in the mouse spinal cordM P Matise
Howard Hughes Medical Institute and Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
Development 126:3649-59. 1999.... - Engrailed, Wnt and Pax genes regulate midbrain--hindbrain developmentA L Joyner
Department of Cell Biology, New York University Medical Center, NY 10016 0497, USA
Trends Genet 12:15-20. 1996..By analogy, segmentation of the rest of the brain might best be described in terms of 'genetic units' defined by genetic and transplantation data... - Embryonic origins of ZebrinII parasagittal stripes and establishment of topographic Purkinje cell projectionsR V Sillitoe
Developmental Biology Program, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10021, USA
Neuroscience 162:574-88. 2009..5. These studies show that PC molecular patterning, efferent circuitry, and DCN nucleogenesis occur simultaneously, suggesting a link between these processes... - Identification and characterization of Lbh, a novel conserved nuclear protein expressed during early limb and heart developmentK J Briegel
Howard Hughes Medical Institute, New York University School of Medicine, New York, New York 10016, USA
Dev Biol 233:291-304. 2001..Based on the molecular characteristics and the domain-specific expression pattern, it is possible that Lbh functions in synergy with other genes known to be required for heart and limb development... - Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneuronsM P Matise
New York University Medical School, Skirball Institute of Biomolecular Medicine, Developmental Genetics Program, New York, New York 10016, USA
J Neurosci 17:7805-16. 1997..Rather, it is suggested that En-1 may function to distinguish a subset of interneurons during the later maturation of the spinal cord... - Early anterior/posterior patterning of the midbrain and cerebellumA Liu
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
Annu Rev Neurosci 24:869-96. 2001..Subsequently, we discuss the molecular genetic studies that have revealed the roles for many genes in normal early patterning of this region. Finally, some of the remaining questions and future directions are discussed... - FGF17b and FGF18 have different midbrain regulatory properties from FGF8b or activated FGF receptorsAimin Liu
Howard Hughes Medical Institute, Developmental Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Development 130:6175-85. 2003..As Fgf17 and Fgf18 are co-expressed with Fgf8 in many tissues, our studies have broad implications for how these FGFs differentially control development... - Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mappingSema K Sgaier
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Neuron 45:27-40. 2005..Thus, we show that progressive changes in the axes of the cerebellum underlie its genesis... - The HD mutation causes progressive lethal neurological disease in mice expressing reduced levels of huntingtinW Auerbach
Skirball Institute of Biomolecular Medicine and Howard Hughes Medical Institute, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Hum Mol Genet 10:2515-23. 2001..These results raise the possibility that therapeutic elimination of huntingtin in HD patients could lead to unintended neurological, as well as developmental side-effects... - Cell behaviors and genetic lineages of the mesencephalon and rhombomere 1Mark Zervas
Howard Hughes Medical Institute, Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Neuron 43:345-57. 2004..Finally, we also uncovered transient and differential genetic lineages of ventral midbrain dopaminergic and ventral hindbrain serotonergic neuronal precursors with respect to Wnt1 and Gli1 expression... - Changing requirements for Gbx2 in development of the cerebellum and maintenance of the mid/hindbrain organizerJames Y H Li
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York, NY 10016, USA
Neuron 36:31-43. 2002..Our work has uncovered distinct requirements for Gbx2 during cerebellum formation and provided a model for how a transcription factor can play multiple roles during development... - Strain-dependent differences in the efficiency of transgenic mouse productionAnna B Auerbach
Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, NYU School of Medicine, 540 First Avenue, New York, NY 10016, USA
Transgenic Res 12:59-69. 2003..Based on our studies, the procedure for transgenic mouse production can be modified for each egg donor strain in order to overcome any deficiencies, and thus to increase the overall efficiency of transgenic mouse production... - How does Fgf signaling from the isthmic organizer induce midbrain and cerebellum development?Tatsuya Sato
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
Dev Growth Differ 46:487-94. 2004..A lower level of signaling transduced by Fgf8a, Fgf17 and Fgf18 induce midbrain development. Numerous feedback loops then maintain appropriate mesencephalon/rhombomere1 and organizer gene expression... - Spatial pattern of sonic hedgehog signaling through Gli genes during cerebellum developmentJoMichelle D Corrales
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, 540 First Avenue, New York, NY 10016, USA
Development 131:5581-90. 2004..Taken together, these studies demonstrate that positive Shh signaling through Gli2 is required to generate a sufficient number of GCPs for proper lobe growth... - Cerebellum morphogenesis: the foliation pattern is orchestrated by multi-cellular anchoring centersAnamaria Sudarov
Developmental Biology Program, Sloan Kettering Institute, York Avenue, New York, NY 10021, USA
Neural Dev 2:26. 2007..Since folia in mammals likely serve as a broad platform on which the anterior-posterior organization of the sensory-motor circuits of the cerebellum are built, it is important to understand how such complex morphology arises... - Sonic hedgehog regulates Gli activator and repressor functions with spatial and temporal precision in the mid/hindbrain regionSandra Blaess
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, 540 First Avenue, New York, NY 10016, USA
Development 133:1799-809. 2006..Thus, the precise spatial and temporal regulation of Gli2A and Gli3R by Shh is instrumental in coordinating mid/hindbrain development in three dimensions... - Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell linesW Auerbach
Skirball Institute of Biomolecular Medicine, New York University School of Medicine, NY 10016, USA
Biotechniques 29:1024-8, 1030, 1032. 2000..C56BL/6-derived ES cell lines, however, have a greater tendency than 129-derived ES cell lines to lose their ability to colonize the germline... - Expression of the mouse Gli and Ptc genes is adjacent to embryonic sources of hedgehog signals suggesting a conservation of pathways between flies and miceK A Platt
The Skirball Institute of Biomolecular Medicine Developmental Genetics Program and Department of Cell Biology, Physiology and Neuroscience, New York University, NY 10016, USA
Mech Dev 62:121-35. 1997..These results are consistent with conservation of the Hh signal transduction pathway in mice with Gli potentially mediating Hh signaling in multiple regions of the developing embryo... - Genetic subdivision of the tectum and cerebellum into functionally related regions based on differential sensitivity to engrailed proteinsSema K Sgaier
Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Development 134:2325-35. 2007..Our study suggests that an ;engrailed code' is integral to partitioning the tectum and cerebellum into functional domains... - The level of sonic hedgehog signaling regulates the complexity of cerebellar foliationJoMichelle D Corrales
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, 540 First Avenue New York, NY 10016, USA
Development 133:1811-21. 2006..Our studies have uncovered a mechanism by which the level and length of Shh signaling could be integral to determining the distinct number of fissures in each species... - Engrailed homeobox genes determine the organization of Purkinje cell sagittal stripe gene expression in the adult cerebellumRoy V Sillitoe
Developmental Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
J Neurosci 28:12150-62. 2008..Our studies reveal that En1/2 are fundamental components of the genetic pathways that pattern the two intersecting coordinate systems of the Cb, morphological divisions and the molecular code... - Gli3 coordinates three-dimensional patterning and growth of the tectum and cerebellum by integrating Shh and Fgf8 signalingSandra Blaess
Developmental Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 511, New York, NY 10021, USA
Development 135:2093-103. 2008..0) through regulation of cell proliferation and viability. In conclusion, our results show that Gli3 is essential for the coordinated three-dimensional patterning and growth of the dorsal mes/r1... - New regulatory interactions and cellular responses in the isthmic organizer region revealed by altering Gbx2 expressionJames Y H Li
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Development 132:1971-81. 2005..Our studies provide new insights into the regulatory interactions that maintain isthmic organizer gene expression and the consequences of altered levels of organizer gene expression on cell survival... - Structural basis by which alternative splicing modulates the organizer activity of FGF8 in the brainShaun K Olsen
Department of Pharmacology, New York University School of Medicine, New York, New York 10016, USA
Genes Dev 20:185-98. 2006..Consistent with the indispensable role of FGF8 in embryonic development, we show that the FGF8 mode of receptor binding appeared as early as in nematodes and has been preserved throughout evolution... - The duration of Fgf8 isthmic organizer expression is key to patterning different tectal-isthmo-cerebellum structuresTatsuya Sato
Developmental Biology Program, Sloan Kettering Institute, 1275 York Avenue, Box 511, New York, NY 10021, USA
Development 136:3617-26. 2009..Thus, the duration as well as the strength of Fgf8 signaling is key to patterning of the mid/hindbrain region. By extrapolation, the length of Fgf8 expression could be crucial to Fgf8 function in other embryonic organizers... - The Engrailed homeobox genes determine the different foliation patterns in the vermis and hemispheres of the mammalian cerebellumYulan Cheng
Developmental Biology Program, Sloan Kettering Institute, New York, NY 10065, USA
Development 137:519-29. 2010..Thus, the En genes represent a new class of genes that are fundamental to patterning cerebellum foliation throughout the mediolateral axis and that act late in development... - Morphogen to mitogen: the multiple roles of hedgehog signalling in vertebrate neural developmentMarc Fuccillo
Developmental Genetics Program and the Department of Cell Biology, Skirball Institute of Biomolecular Medicine, New York University Medical Center, 540 First Avenue, New York, NY 10016, USA
Nat Rev Neurosci 7:772-83. 2006..Here we review our present knowledge of the hedgehog signalling pathway and speculate about areas in which further insights into this versatile pathway might be forthcoming... - Engrailed homeobox genes regulate establishment of the cerebellar afferent circuit mapRoy V Sillitoe
Developmental Biology Program, Sloan Kettering Institute, 1275 York Avenue, New York, NY 10021, USA
J Neurosci 30:10015-24. 2010..In summary, our data suggest that En1/2 are master regulators of three-dimensional organization of the cerebellum and coordinately regulate morphology, patterned gene expression, and afferent topography... - Sonic hedgehog regulates discrete populations of astrocytes in the adult mouse forebrainA Denise R Garcia
Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, New York, New York 10065, USA
J Neurosci 30:13597-608. 2010..Collectively, our findings demonstrate a role for neuron-derived Shh in regulating specific populations of differentiated astrocytes... - All mouse ventral spinal cord patterning by hedgehog is Gli dependent and involves an activator function of Gli3C Brian Bai
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Dev Cell 6:103-15. 2004..Therefore, in the spinal cord all Hh signaling is Gli dependent. Furthermore, a combination of Gli2 and Gli3 is required to regulate motor neuron development and spatial patterning of ventral spinal cord progenitors... - Dynamic changes in the response of cells to positive hedgehog signaling during mouse limb patterningSohyun Ahn
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York, NY 10016, USA
Cell 118:505-16. 2004..Finally, by fate mapping Shh-responding cells in Gli2 and Gli3 mutant limbs, we demonstrate that a specific level of positive Hh signaling is not required to specify digit identities... - Gli2, but not Gli1, is required for initial Shh signaling and ectopic activation of the Shh pathwayC Brian Bai
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Development 129:4753-61. 2002..Our studies demonstrate that, in mammals, Gli1 is not required for Shh signaling and that Gli2 mediates inappropriate activation of the pathway due to loss of the negative regulator Ptc... - Fate map of mouse ventral limb ectoderm and the apical ectodermal ridgeQiuxia Guo
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
Dev Biol 264:166-78. 2003..These studies have uncovered new aspects of the cellular mechanisms involved in AER formation and in partitioning the ventral ectoderm in mouse limb... 
Hedgehog signaling plays a cell-autonomous role in maximizing cardiac developmental potentialNatalie A Thomas
Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
Development 135:3789-99. 2008..Thus, Hh signaling plays an essential early role in defining the optimal number of cardiomyocytes, making it an attractive target for manipulation of multipotent progenitor cells...- Congenital heart disease reminiscent of partial trisomy 2p syndrome in mice transgenic for the transcription factor LbhKaroline J Briegel
Howard Hughes Medical Institute and Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
Development 132:3305-16. 2005..Our findings implicate LBH as a candidate gene for CHD associated with partial trisomy 2p syndrome and suggest an important role of Lbh in transcriptional control during normal cardiogenesis... - Induction of medulloblastomas in mice by sonic hedgehog, independent of Gli1Howard L Weiner
Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
Cancer Res 62:6385-9. 2002..We have developed an efficient mouse model of medulloblastoma and shown that Gli1 is not required for tumorigenesis when Shh signaling is activated upstream in the pathway... - In vivo analysis of quiescent adult neural stem cells responding to Sonic hedgehogSohyun Ahn
Howard Hughes Medical Institute, Developmental Genetics Program, Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA
Nature 437:894-7. 2005..Furthermore, we show that the neural stem cell niches in the subventricular zone and dentate gyrus are established sequentially and not until late embryonic stages... - Genetic fate mapping using site-specific recombinasesEmilie Legue
Memorial Sloan Kettering Cancer Center, New York, USA
Methods Enzymol 477:153-81. 2010.... - Morphology, molecular codes, and circuitry produce the three-dimensional complexity of the cerebellumRoy V Sillitoe
Developmental Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
Annu Rev Cell Dev Biol 23:549-77. 2007..Finally, we discuss how abnormal cerebellar organization may result in neurological disorders like autism... - In vivo MRI of neural cell migration dynamics in the mouse brainBrian J Nieman
Kimmel Center for Biology and Medicine at the Skirball Institute of Biomolecular Medicine, New York University School of Medicine, NY, NY 10016, USA
Neuroimage 50:456-64. 2010..The use of MRI in future studies tracking endogenous NB cells will permit a more complete evaluation of their role during homeostasis at various developmental stages and during disease progression... - Beta-catenin activation is necessary and sufficient to specify the dorsal dermal fate in the mouseRadhika Atit
Department of Genetics, Case Western Reserve University, Cleveland, OH 44106, USA
Dev Biol 296:164-76. 2006..Our results indicate that the mouse central dermomyotome gives rise to dermis, muscle, and brown fat, and that Wnt signalling normally instructs cells to select the dorsal dermal fate... - Specific regions within the embryonic midbrain and cerebellum require different levels of FGF signaling during developmentM Albert Basson
Department of Anatomy and Program in Developmental Biology, University of California San Francisco, CA 94158, USA
Development 135:889-98. 2008..We suggest a molecular explanation for this phenomenon by providing evidence that FGF signaling functions to inhibit the BMP signaling that promotes roof plate development... - Sonic hedgehog signaling regulates Gli2 transcriptional activity by suppressing its processing and degradationYong Pan
Weill Medical College of Cornell University, 1300 York Avenue, Room W404, New York, NY 10021, USA
Mol Cell Biol 26:3365-77. 2006..Both processing and degradation of Gli2 are suppressed by Shh signaling in vivo. Our findings provide the first demonstration of a molecular mechanism by which the Gli2 transcriptional activity is regulated by Shh signaling... - The exon 8-containing prosaposin gene splice variant is dispensable for mouse development, lysosomal function, and secretionTsadok Cohen
Department of Cell Research and Immunology, Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
Mol Cell Biol 25:2431-40. 2005....
Research Grants
- ROLES OF MOUSE GLI GENES IN HEDGEHOG SIGNALINGAlexandra Joyner; Fiscal Year: 2007....
- ROLES OF MOUSE GLI GENES IN HEDGEHOG SIGNALINGAlexandra Joyner; Fiscal Year: 2006..4. To determine whether some cells receiving Shh signaling move away from the source of Shh and thus receive a shorter exposure to Shh than other cells using a Cre/loxP fate mapping approach with Gli1- CreEr mice. ..
- Fgf8 Function in Midbrain/r1 Borders and PatterningAlexandra Joyner; Fiscal Year: 2006..Does Fgf8 induces different midbrain and hindbrain structures along the anterior/posterior axis directly through setting up a gradient of Fgf proteins? 5. Is Fgf8 or Wnt1 signaling required to maintain compartment borders? ..