Hieronim Jakubowski

Summary

Affiliation: New Jersey
Country: USA

Publications

  1. ncbi request reprint Protective mechanisms against homocysteine toxicity: the role of bleomycin hydrolase
    Jaroslaw Zimny
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, International Center for Public Health, Newark, New Jersey 07101, USA
    J Biol Chem 281:22485-92. 2006
  2. doi request reprint Quality control in tRNA charging
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ, USA
    Wiley Interdiscip Rev RNA 3:295-310. 2012
  3. ncbi request reprint Chemical biology of homocysteine thiolactone and related metabolites
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, New Jersey, USA
    Adv Clin Chem 55:81-103. 2011
  4. ncbi request reprint Anti-N-homocysteinylated protein autoantibodies and cardiovascular disease
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101 1709, USA
    Clin Chem Lab Med 43:1011-4. 2005
  5. ncbi request reprint Pathophysiological consequences of homocysteine excess
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    J Nutr 136:1741S-1749S. 2006
  6. ncbi request reprint Mechanism of the condensation of homocysteine thiolactone with aldehydes
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey, Medical School, Newark, NJ 07101, USA
    Chemistry 12:8039-43. 2006
  7. ncbi request reprint The molecular basis of homocysteine thiolactone-mediated vascular disease
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101 1709, USA
    Clin Chem Lab Med 45:1704-16. 2007
  8. ncbi request reprint Facile syntheses of [35S]homocysteine-thiolactone, [35S]homocystine, [35S]homocysteine, and [S-nitroso-35S]homocysteine
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    Anal Biochem 370:124-6. 2007
  9. doi request reprint New method for the determination of protein N-linked homocysteine
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    Anal Biochem 380:257-61. 2008
  10. doi request reprint Mutations in cystathionine beta-synthase or methylenetetrahydrofolate reductase gene increase N-homocysteinylated protein levels in humans
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, 225 Warren St, Newark, NJ 07101 1709, USA
    FASEB J 22:4071-6. 2008

Collaborators

Detail Information

Publications60

  1. ncbi request reprint Protective mechanisms against homocysteine toxicity: the role of bleomycin hydrolase
    Jaroslaw Zimny
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, International Center for Public Health, Newark, New Jersey 07101, USA
    J Biol Chem 281:22485-92. 2006
    ..Our data suggest that BLH protects cells against Hcy toxicity by hydrolyzing intracellular Hcy-thiolactone...
  2. doi request reprint Quality control in tRNA charging
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ, USA
    Wiley Interdiscip Rev RNA 3:295-310. 2012
    ..Elevated levels of mistranslation and the synthesis of statistical proteins can be beneficial for pathogens by increasing phenotypic variation essential for the evasion of host defenses...
  3. ncbi request reprint Chemical biology of homocysteine thiolactone and related metabolites
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, New Jersey, USA
    Adv Clin Chem 55:81-103. 2011
    ..This chapter reviews fundamental biological chemistry of Hcy-thiolactone, N-Hcy-protein, and N epsilon-Hcy-Lys and discusses their clinical significance...
  4. ncbi request reprint Anti-N-homocysteinylated protein autoantibodies and cardiovascular disease
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101 1709, USA
    Clin Chem Lab Med 43:1011-4. 2005
    ....
  5. ncbi request reprint Pathophysiological consequences of homocysteine excess
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    J Nutr 136:1741S-1749S. 2006
    ..Potential utility of Hcy-thiolactone, N-Hcy-protein, or anti-N-Hcy-protein autoantibodies as markers of Hcy excess is discussed...
  6. ncbi request reprint Mechanism of the condensation of homocysteine thiolactone with aldehydes
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey, Medical School, Newark, NJ 07101, USA
    Chemistry 12:8039-43. 2006
    ..The facile formation of tetrahydrothiazines may contribute to HTL elimination from the human body...
  7. ncbi request reprint The molecular basis of homocysteine thiolactone-mediated vascular disease
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101 1709, USA
    Clin Chem Lab Med 45:1704-16. 2007
    ..This article reviews recent evidence supporting the hypothesis that Hcy-thiolactone contributes to pathophysiological effects of Hcy on the vascular system...
  8. ncbi request reprint Facile syntheses of [35S]homocysteine-thiolactone, [35S]homocystine, [35S]homocysteine, and [S-nitroso-35S]homocysteine
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    Anal Biochem 370:124-6. 2007
  9. doi request reprint New method for the determination of protein N-linked homocysteine
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    Anal Biochem 380:257-61. 2008
    ..0127-0.0828 vs. 0.0027-0.0086 mol/mol). Normal human plasma and milk were found to contain submicromolar concentrations of protein N-linked Hcy, whereas cow milk and whey contained micromolar concentrations of protein N-linked Hcy...
  10. doi request reprint Mutations in cystathionine beta-synthase or methylenetetrahydrofolate reductase gene increase N-homocysteinylated protein levels in humans
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, 225 Warren St, Newark, NJ 07101 1709, USA
    FASEB J 22:4071-6. 2008
    ..We also show that CBS-deficient patients have significantly elevated plasma levels of prothrombotic N-Hcy-fibrinogen. These results provide a possible explanation for increased atherothrombosis observed in CBS-deficient patients...
  11. pmc Genetic or nutritional disorders in homocysteine or folate metabolism increase protein N-homocysteinylation in mice
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, 225 Warren St, Newark, NJ 07101 1709, USA
    FASEB J 23:1721-7. 2009
    ..These findings provide evidence that N-Hcy-protein is an important metabolite associated with Hcy pathophysiology in the mouse...
  12. ncbi request reprint The pathophysiological hypothesis of homocysteine thiolactone-mediated vascular disease
    H Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    J Physiol Pharmacol 59:155-67. 2008
    ..We conclude that genetic or dietary hyperhomocysteinemia significantly elevates proatherothrombotic metabolites Hcy-thiolactone and N-Hcy-proteins in humans and mice...
  13. doi request reprint The role of paraoxonase 1 in the detoxification of homocysteine thiolactone
    Hieronim Jakubowski
    Department of Biochemistry and Biotechnology, University of Life Sciences, Poznan, Poland
    Adv Exp Med Biol 660:113-27. 2010
    ..This article outlines Hcy-thiolactone pathobiology and reviews evidence documenting the role of PON1 in minimizing Hcy-thiolactone and N-Hcy-protein accumulation...
  14. ncbi request reprint Quality control in tRNA charging -- editing of homocysteine
    Hieronim Jakubowski
    UMDNJ New Jersey Medical School, Department of Microbiology and Molecular Genetics, International Center for Public Health, Newark, USA
    Acta Biochim Pol 58:149-63. 2011
    ..Excessive homocysteine thiolactone synthesis in hyperhomocysteinemia caused by genetic or nutritional deficiencies is linked to human vascular and neurological diseases...
  15. ncbi request reprint Molecular basis of homocysteine toxicity in humans
    H Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, 225 Warren Street, Newark, New Jersey 07101, USA
    Cell Mol Life Sci 61:470-87. 2004
    ..The ability of Hcy to interfere with protein biosynthesis, which causes protein damage, induces cell death and elicits immune response, is likely to contribute to the pathology of human disease...
  16. ncbi request reprint Homocysteine-thiolactone and S-nitroso-homocysteine mediate incorporation of homocysteine into protein in humans
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark 07101 1709, USA
    Clin Chem Lab Med 41:1462-6. 2003
    ..Hcy-thiolactone is present in human plasma. Modification with Hcy-thiolactone leads to protein damage. Hcy-thiolactone is detoxified by Hcy-thiolactonase/paraoxonase present in a subset of high-density lipoprotein particles in humans...
  17. ncbi request reprint Aminoacylation of coenzyme A and pantetheine by aminoacyl-tRNA synthetases: possible link between noncoded and coded peptide synthesis
    H Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, 185 South Orange Avenue, Newark, New Jersey 07103, USA
    Biochemistry 37:5147-53. 1998
    ....
  18. ncbi request reprint Metabolism of homocysteine thiolactone in human cell cultures. Possible mechanism for pathological consequences of elevated homocysteine levels
    H Jakubowski
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, Newark, New Jersey 07103, USA
    J Biol Chem 272:1935-42. 1997
    ..The metabolic conversion of homocysteine to homocysteine thiolactone and the reactivity of the thiolactone toward proteins may explain pathological consequences of elevated levels of homocysteine such as observed in vascular disease...
  19. ncbi request reprint Translational accuracy of aminoacyl-tRNA synthetases: implications for atherosclerosis
    H Jakubowski
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA
    J Nutr 131:2983S-7S. 2001
    ....
  20. ncbi request reprint Homocysteine is a protein amino acid in humans. Implications for homocysteine-linked disease
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    J Biol Chem 277:30425-8. 2002
    ..3 to 23% of total homocysteine. Thus, Hcy-N-protein is a significant component of homocysteine metabolism in humans, possibly contributing to adverse effects of homocysteine on human cells...
  21. ncbi request reprint Protein homocysteinylation: possible mechanism underlying pathological consequences of elevated homocysteine levels
    H Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
    FASEB J 13:2277-83. 1999
    ..Jakubowski, H. Protein homocysteinylation: possible mechanism underlying pathological consequences of elevated homocysteine levels...
  22. ncbi request reprint Genetic determinants of homocysteine thiolactonase activity in humans: implications for atherosclerosis
    H Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA
    FEBS Lett 491:35-9. 2001
    ..High thiolactonase activity afforded better protection against protein homocysteinylation than low thiolactonase activity. These results suggest that variations in HTase may play a role in Hcy-associated cardiovascular disease...
  23. ncbi request reprint The determination of homocysteine-thiolactone in biological samples
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark 07101, USA
    Anal Biochem 308:112-9. 2002
    ..The data support the conclusion that homocysteine-thiolactone is a ubiquitous metabolite whose levels are directly related to homocysteine levels...
  24. ncbi request reprint Aminoacyl thioester chemistry of class II aminoacyl-tRNA synthetases
    H Jakubowski
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, 185 South Orange Avenue, Newark, New Jersey 07103, USA
    Biochemistry 36:11077-85. 1997
    ..That the thiol-binding subsite exists also in AspRS and SerRS, which do not need editing function, suggests that these class II enzymes possess vestigial editing functions...
  25. ncbi request reprint Homocysteine thiolactone and protein homocysteinylation in human endothelial cells: implications for atherosclerosis
    H Jakubowski
    Department of Microbiology, UMDNJ New Jersey Medical School, Newark, NJ, USA
    Circ Res 87:45-51. 2000
    ..These data support a hypothesis that metabolic conversion of homocysteine to thiolactone and protein homocysteinylation by thiolactone may play a role in homocysteine-induced vascular damage...
  26. ncbi request reprint Translational incorporation of S-nitrosohomocysteine into protein
    H Jakubowski
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, New Jersey 07103, USA
    J Biol Chem 275:21813-6. 2000
    ..S-Nitrosylation-mediated translational incorporation of Hcy into protein may occur under natural conditions in cells and contribute to Hcy-induced pathogenesis in atherosclerosis...
  27. ncbi request reprint Calcium-dependent human serum homocysteine thiolactone hydrolase. A protective mechanism against protein N-homocysteinylation
    H Jakubowski
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, New Jersey 07103, USA
    J Biol Chem 275:3957-62. 2000
    ..By detoxifying homocysteine thiolactone, the thiolactonase/paraoxonase would protect proteins against homocysteinylation, a potential contributing factor to atherosclerosis...
  28. ncbi request reprint Misacylation of tRNALys with noncognate amino acids by lysyl-tRNA synthetase
    H Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, Newark 07103, USA
    Biochemistry 38:8088-93. 1999
    ..A novel feature of this editing reaction is that ornithine lactam is formed by the facile cyclization of ornithyl adenylate...
  29. ncbi request reprint Metabolism of homocysteine-thiolactone in plants
    Hieronim Jakubowski
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey UMDNJ New Jersey Medical School, International Center for Public Health, Newark, New Jersey 07101, USA
    J Biol Chem 278:6765-70. 2003
    ..Thus, Hcy-thiolactone and Hcy bound to protein by an amide (or peptide) linkage (Hcy-N-protein) are significant components of plant Hcy metabolism...
  30. ncbi request reprint Protein N-homocysteinylation: implications for atherosclerosis
    H Jakubowski
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, Newark 07103, USA
    Biomed Pharmacother 55:443-7. 2001
    ..Variations in Hcy thiolactonase may play an important role in Hcy-associated human cardiovascular disease...
  31. doi request reprint Immunohistochemical detection of N-homocysteinylated proteins in humans and mice
    Joanna Perła-Kaján
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland
    Biomed Pharmacother 62:473-9. 2008
    ..In conclusion, polyclonal rabbit anti-N-Hcy-protein antibody can detect and monitor N-homocysteinylated proteins in human and mouse tissues with good sensitivity and specificity...
  32. ncbi request reprint Mutations in methylenetetrahydrofolate reductase or cystathionine beta-synthase gene, or a high-methionine diet, increase homocysteine thiolactone levels in humans and mice
    Grazyna Chwatko
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, 225 Warren St, Newark, NJ 07101 1709, USA
    FASEB J 21:1707-13. 2007
    ..7-fold and 25-fold increases in plasma and urinary Hcy-thiolactone levels, respectively. Thus, we conclude that hyperhomocysteinemia leads to significant increases in the atherogenic metabolite Hcy-thiolactone in humans and mice...
  33. pmc Metabolism and neurotoxicity of homocysteine thiolactone in mice: evidence for a protective role of paraoxonase 1
    Kamila Borowczyk
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    J Alzheimers Dis 30:225-31. 2012
    ....
  34. doi request reprint Homocysteine editing and growth inhibition in Escherichia coli
    Marta Sikora
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    Microbiology 155:1858-65. 2009
    ..We also show that the levels of protein N-linked Hcy modestly increase in E. coli cells in Hcy-supplemented cultures. The results suggest that Hcy editing restrains bacterial growth...
  35. doi request reprint Metabolism and neurotoxicity of homocysteine thiolactone in mice: protective role of bleomycin hydrolase
    Kamila Borowczyk
    Department of Microbiology and Molecular Genetics, International Center for Public Health, UMDNJ New Jersey Medical School, 225 Warren Street, Newark, NJ 07101, USA
    Amino Acids 43:1339-48. 2012
    ....
  36. pmc Design and properties of efficient tRNA:EF-Tu FRET system for studies of ribosomal translation
    Maxim Chudaev
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, Newark, NJ 07101, USA
    Protein Eng Des Sel 26:347-57. 2013
    ..The results obtained demonstrate that the EF-Tu:tRNA FRET system described can be used for investigations of ribosomal translation in many types of experiments...
  37. ncbi request reprint Paraoxonase 1 and homocysteine metabolism
    Joanna Perła-Kaján
    Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland
    Amino Acids 43:1405-17. 2012
    ..The links between PON1 and Hcy in relation to pathological states such as coronary artery disease, stroke, diabetic mellitus, kidney failure and Alzheimer's disease that emerge from recent studies are the topics of this review...
  38. doi request reprint Direct monitoring of albumin lysine-525 N-homocysteinylation in human serum by liquid chromatography/mass spectrometry
    Marta Sikora
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61 704 Poznan, Poland
    Anal Biochem 405:132-4. 2010
    ..Using this method, we found that the extent of lysine-525 N-homocysteinylation was significantly increased in patients with cystathionine beta-synthase deficiency...
  39. doi request reprint Paraoxonase 1 protects against protein N-homocysteinylation in humans
    Joanna Perla-Kajan
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, 225 Warren St Newark, NJ 07101 1709, USA
    FASEB J 24:931-6. 2010
    ..Perła-Kaján, J., Jakubowski, H. Paraoxonase 1 protects against protein N-homocysteinylation in humans...
  40. ncbi request reprint Cross-talk between Cys34 and lysine residues in human serum albumin revealed by N-homocysteinylation
    Rafal Glowacki
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, International Center for Public Health, Newark, New Jersey 07103, USA
    J Biol Chem 279:10864-71. 2004
    ....
  41. pmc Properties of Escherichia coli EF-Tu mutants designed for fluorescence resonance energy transfer from tRNA molecules
    Joanna Perla-Kajan
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, Newark, 07101, USA
    Protein Eng Des Sel 23:129-36. 2010
    ..The results indicate that at least three EF-Tu mutants, K324C, G325C and E348C, are suitable for further studies. Remarkably, GST fusions that were not cleaved were also active in the various assays, despite the N-terminal fusion...
  42. ncbi request reprint Autoantibodies against N-homocysteinylated proteins in humans: implications for atherosclerosis
    Anetta Undas
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    Stroke 35:1299-304. 2004
    ..This study was undertaken to provide evidence for anti-Nepsilon-Hcy-Lys-protein antibody and to examine associations between the antibody level, Hcy, and stroke in humans...
  43. doi request reprint Analysis of site-specific N-homocysteinylation of human serum albumin in vitro and in vivo using MALDI-ToF and LC-MS/MS mass spectrometry
    Lukasz Marczak
    Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12 14, Poznan, Poland
    J Proteomics 74:967-74. 2011
    ....
  44. ncbi request reprint The determination of homocysteine-thiolactone in human plasma
    Grazyna Chwatko
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07103, USA
    Anal Biochem 337:271-7. 2005
    ..82+/-6.13 nM. In 29 of the 60 human plasma samples analyzed, homocysteine-thiolactone levels were below the detection limit. Homocysteine-thiolactone represented from 0 to 0.28%, on average 0.023+/-0.05%, of plasma total homocysteine...
  45. ncbi request reprint Purification of antibodies against N-homocysteinylated proteins by affinity chromatography on Nomega-homocysteinyl-aminohexyl-Agarose
    Joanna Perła
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 807:257-61. 2004
    ..6 and eluted with a pH 2.3 buffer. Enzyme-linked immunosorbent assays demonstrate that the antibody recognizes specifically N-homocysteinylated variants of hemoglobin, albumin, transferrin, and antitrypsin...
  46. doi request reprint Plasma total homocysteine is a determinant of carotid intima-media thickness and circulating endothelial progenitor cells in patients with newly diagnosed hypertension
    Paweł Bogdański
    Department of Internal Medicine, Metabolic Disorders and Hypertension, Poznan University of Medical Sciences, Poznan, Poland
    Clin Chem Lab Med 50:1107-13. 2012
    ..Our objective was to examine a hypothesis that Hcy is a predictor of total antioxidant status (TAS) and endothelial progenitor cells (EPCs), important in the repair of injured endothelium, in hypertensive patients...
  47. pmc Cation exchange HPLC analysis of desmosines in elastin hydrolysates
    Joanna Perła-Kaján
    Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, 60 637 Poznan, Poland
    Anal Bioanal Chem 401:2473-9. 2011
    ..We also found that desmosines levels were lower in lung elastin isolated from hyperhomocysteinemic Pcft(-/-) mice deficient in intestinal folate transport compared with wild-type Pcft(+/+) animals...
  48. ncbi request reprint Urinary excretion of homocysteine-thiolactone in humans
    Grazyna Chwatko
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark 07103, USA
    Clin Chem 51:408-15. 2005
    ..We examined the hypothesis that the human body eliminates Hcy-thiolactone by urinary excretion...
  49. doi request reprint Identification of N-homocysteinylation sites in plasma proteins
    Marta Sikora
    Polish Academy of Sciences, Institute of Bioorganic Chemistry, Poznan, Poland
    Amino Acids 46:235-44. 2014
    ..N-Hcy-Lys562, N-Hcy-Lys344, and N-Hcy-Lys385 were identified in human fibrinogen from patients with cystathionine β-synthase deficiency. The protocol can be completed in 4 days...
  50. doi request reprint Reduced homocysteine-thiolactonase activity in Alzheimer's disease
    Joanna Suszynska
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07101 1709, USA
    J Alzheimers Dis 19:1177-83. 2010
    ..Levels of brain total Hcy and N-linked protein Hcy did not differ between AD cases and controls. These results suggest that diminished functional BLH activity could contribute to the pathology of AD...
  51. ncbi request reprint Folic acid administration and antibodies against homocysteinylated proteins in subjects with hyperhomocysteinemia
    Anetta Undas
    Institute of Cardiology, Jagiellonian University of Medicine, Cracow, Poland
    Thromb Haemost 96:342-7. 2006
    ..These observations might explain at least in part the failure of vitamin therapy to reduce the risk of cardiovascular events as recently reported...
  52. ncbi request reprint The effects of age and hyperhomocysteinemia on the redox forms of plasma thiols
    Danila Di Giuseppe
    Department of Neuroscience, Pharmacology Unit, University of Sienna, Sienna, Italy
    J Lab Clin Med 144:235-45. 2004
    ....
  53. ncbi request reprint Antibodies to N-homocysteinylated albumin as a marker for early-onset coronary artery disease in men
    Anetta Undas
    Department of Medicine, Jagiellonian University School of Medicine, 8 Skawinska Street, 31 066 Cracow, Poland
    Thromb Haemost 93:346-50. 2005
    ..An autoimmune response to Nepsilon-Hcy-albumin may represent a novel mechanism involved in the early development of CAD...
  54. pmc Synergistic, random sequential binding of substrates in cobalamin-independent methionine synthase
    Rebecca E Taurog
    Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 45:5083-91. 2006
    ..Methyl transfer has been determined to be slow compared to ternary complex formation and dissociation. Simulations indicate that nearly all of the enzyme is present as the ternary complex under physiological conditions...
  55. ncbi request reprint Modification by homocysteine thiolactone affects redox status of cytochrome C
    Joanna Perła-Kaján
    Institute of Bioorganic Chemistry Polish Academy of Sciences, 61 704 Poznan, Poland
    Biochemistry 46:6225-31. 2007
    ..However, no major secondary structure perturbations were observed as shown by circular dichroism spectroscopy. Our data illustrate how N-homocysteinylation can interfere with the function of heme-containing proteins...
  56. ncbi request reprint Letter by Undas and Jakubowski regarding article, "Relationship between homocysteine and mortality in chronic kidney disease"
    Anetta Undas
    Circulation 114:e547; author reply e548. 2006
  57. ncbi request reprint Prevention of brain disease from severe 5,10-methylenetetrahydrofolate reductase deficiency
    Kevin A Strauss
    Clinic for Special Children, Strasburg, PA 17579, USA
    Mol Genet Metab 91:165-75. 2007
    ..To effectively support the metabolic requirements of rapid brain growth, a large dose of betaine should be started early in life...
  58. ncbi request reprint Plasma homocysteine affects fibrin clot permeability and resistance to lysis in human subjects
    Anetta Undas
    Institute of Cardiology, Jagiellonian University School of Medicine, Krakow, Poland
    Arterioscler Thromb Vasc Biol 26:1397-404. 2006
    ..Homocysteine (Hcy) is a risk factor for thrombosis. We investigated a hypothesis that the clot permeability and its resistance to fibrinolysis is associated with plasma total Hcy (tHcy) in human subjects...
  59. ncbi request reprint [The role of homocysteine thiolactone in some of human diseases]
    Marta Sikora
    Instytut Chemii Bioorganicznej PAN, Poznan
    Postepy Biochem 52:417-23. 2006
    ..We also describe the effect of vitamin therapy (folic acid, vitamins: B6, B12) used for lowering the homocysteine level in humans as well...
  60. ncbi request reprint Differential regulation of homocysteine transport in vascular endothelial and smooth muscle cells
    Xiaohua Jiang
    Temple University School of Medicine, Department of Pharmacology, 3420 North Broad Street, Philadelphia, PA 19140, USA
    Arterioscler Thromb Vasc Biol 27:1976-83. 2007
    ..This study characterized and directly compared Hcy transport in cultured human aortic ECs (HAECs) and smooth muscle cells (HASMCs)...