Masayori Inouye

Summary

Affiliation: New Jersey
Country: USA

Publications

  1. ncbi request reprint Signaling by transmembrane proteins shifts gears
    Masayori Inouye
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Cell 126:829-31. 2006
  2. doi request reprint mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systems
    Yoshihiro Yamaguchi
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Prog Mol Biol Transl Sci 85:467-500. 2009
  3. pmc The expression profile of microRNAs in mouse embryos
    Junichi Mineno
    Center for Cell and Gene Therapy, Takara Bio Inc, Seta 3 4 1, Otsu, Shiga 520 2193, Japan
    Nucleic Acids Res 34:1765-71. 2006
  4. pmc Dissection of 16S rRNA methyltransferase (KsgA) function in Escherichia coli
    Koichi Inoue
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Bacteriol 189:8510-8. 2007
  5. ncbi request reprint Complex formation between a putative 66-residue thumb domain of bacterial reverse transcriptase RT-Ec86 and the primer recognition RNA
    Masayori Inouye
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 279:50735-42. 2004
  6. ncbi request reprint The discovery of mRNA interferases: implication in bacterial physiology and application to biotechnology
    Masayori Inouye
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Cell Physiol 209:670-6. 2006
  7. ncbi request reprint Functional and structural characterization of EnvZ, an osmosensing histidine kinase of E. coli
    Takeshi Yoshida
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ, USA
    Methods Enzymol 423:184-202. 2007
  8. ncbi request reprint Efficient condensed-phase production of perdeuterated soluble and membrane proteins
    William M Schneider
    Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    J Struct Funct Genomics 11:143-54. 2010
  9. ncbi request reprint Characterization of ChpBK, an mRNA interferase from Escherichia coli
    Yonglong Zhang
    Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    J Biol Chem 280:26080-8. 2005
  10. pmc Cysteine-scanning analysis of the dimerization domain of EnvZ, an osmosensing histidine kinase
    Ling Qin
    Department of Biochemistry, UMDNJ Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Bacteriol 185:3429-35. 2003

Research Grants

  1. Cold Shock Vectors for Single Protein Production
    Masayori Inouye; Fiscal Year: 2004
  2. STRESS RESPONSE AND ADAPTATION IN ESCHERICHIA COLI
    Masayori Inouye; Fiscal Year: 2002
  3. STRESS RESPONSE AND ADAPTATION IN ESCHERICHIA COLI
    Masayori Inouye; Fiscal Year: 2000
  4. Propeptide-Mediated Protein Folding
    Masayori Inouye; Fiscal Year: 2006
  5. REVERSE TRANSCRIPTASES IN THE PROKARYOTES
    Masayori Inouye; Fiscal Year: 2004
  6. Propeptide-Mediated Protein Folding
    Masayori Inouye; Fiscal Year: 2004
  7. Propeptide-Mediated Protein Folding
    Masayori Inouye; Fiscal Year: 2005
  8. Propeptide-Mediated Protein Folding
    Masayori Inouye; Fiscal Year: 2005
  9. Signal Transduction by Histidine Kinases and their Response Regulators
    Masayori Inouye; Fiscal Year: 2007
  10. Signal Transduction by Histidine Kinases and their Response Regulators
    Masayori Inouye; Fiscal Year: 2007

Collaborators

Detail Information

Publications88

  1. ncbi request reprint Signaling by transmembrane proteins shifts gears
    Masayori Inouye
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Cell 126:829-31. 2006
    ..In this issue of Cell, Hulko et al. (2006) report the NMR structure of a HAMP domain and present data suggesting that it transduces signals through a simple rotation of its four-helix parallel coiled coil...
  2. doi request reprint mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systems
    Yoshihiro Yamaguchi
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Prog Mol Biol Transl Sci 85:467-500. 2009
    ..We propose that mRNA interferases play roles not only in cell growth regulation and programmed cell death, but also in regulation of specific gene expression (either positively or negatively) in bacteria...
  3. pmc The expression profile of microRNAs in mouse embryos
    Junichi Mineno
    Center for Cell and Gene Therapy, Takara Bio Inc, Seta 3 4 1, Otsu, Shiga 520 2193, Japan
    Nucleic Acids Res 34:1765-71. 2006
    ..Our results indicate existence of a significant number of new miRNAs expressed at specific stages of mammalian embryonic development and which were not detected by earlier methods...
  4. pmc Dissection of 16S rRNA methyltransferase (KsgA) function in Escherichia coli
    Koichi Inoue
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Bacteriol 189:8510-8. 2007
    ..We discuss a possible mechanism of the KsgA-induced acid-sensitive phenotype...
  5. ncbi request reprint Complex formation between a putative 66-residue thumb domain of bacterial reverse transcriptase RT-Ec86 and the primer recognition RNA
    Masayori Inouye
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 279:50735-42. 2004
    ..These results support the notion that the highly diverse C-terminal regions of bacterial RTs play an important role in recognizing their own specific primer-template RNA structure for the cDNA priming reaction...
  6. ncbi request reprint The discovery of mRNA interferases: implication in bacterial physiology and application to biotechnology
    Masayori Inouye
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Cell Physiol 209:670-6. 2006
    ..The discovery of a novel way to interfere with mRNA function by mRNA interferases opens a wide variety of avenues in basic as well as applied and clinical sciences...
  7. ncbi request reprint Functional and structural characterization of EnvZ, an osmosensing histidine kinase of E. coli
    Takeshi Yoshida
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ, USA
    Methods Enzymol 423:184-202. 2007
    ..In this chapter, we describe functional and structural studies of EnvZc, which can be applied to characterize other histidine kinases...
  8. ncbi request reprint Efficient condensed-phase production of perdeuterated soluble and membrane proteins
    William M Schneider
    Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    J Struct Funct Genomics 11:143-54. 2010
    ..The 10-40 fold reduction in costs of fermentation media provided by using a condensed culture system opens the door to many new applications for perdeuterated proteins in spectroscopic and crystallographic studies...
  9. ncbi request reprint Characterization of ChpBK, an mRNA interferase from Escherichia coli
    Yonglong Zhang
    Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    J Biol Chem 280:26080-8. 2005
    ..The ChpBK cleavage of a synthetic RNA substrate generated a 2',3'-cyclic phosphate group at the 3'-end of the 5'-end product and a 5'-OH group at the 5'-end of the 3'-end product in a manner identical to that of MazF...
  10. pmc Cysteine-scanning analysis of the dimerization domain of EnvZ, an osmosensing histidine kinase
    Ling Qin
    Department of Biochemistry, UMDNJ Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Bacteriol 185:3429-35. 2003
    ..The results indicate that some mutations are specific either for the phosphatase activity or for the kinase activity...
  11. ncbi request reprint The design and development of Tar-EnvZ chimeric receptors
    Takeshi Yoshida
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ, USA
    Methods Enzymol 423:166-83. 2007
    ..This chapter describes methods for designing chimeric proteins between a histidine kinase of interest and the Tar receptor and applications of the chimeric protein...
  12. pmc Replacement of all arginine residues with canavanine in MazF-bs mRNA interferase changes its specificity
    Yojiro Ishida
    Department of Biochemistry, Robert Wood Johnson Medical School and Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854, USA
    J Biol Chem 288:7564-71. 2013
    ..Mass spectrometry analysis confirmed that all Arg residues were replaced with Can. The present system offers a novel approach to create new functional proteins by replacing a specific amino acid in a protein with its analogues...
  13. ncbi request reprint Suppression of defective ribosome assembly in a rbfA deletion mutant by overexpression of Era, an essential GTPase in Escherichia coli
    Koichi Inoue
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Microbiol 48:1005-16. 2003
    ..These results indicate that Era is involved in 16S rRNA maturation and ribosome assembly...
  14. ncbi request reprint Assay of transcription antitermination by proteins of the CspA family
    Sangita Phadtare
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane Piscataway, New Jersey 08854 5635, USA
    Methods Enzymol 371:460-71. 2003
  15. pmc A novel membrane-bound toxin for cell division, CptA (YgfX), inhibits polymerization of cytoskeleton proteins, FtsZ and MreB, in Escherichia coli
    Hisako Masuda
    Department of Biochemistry, Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School, Piscataway, NJ, USA
    FEMS Microbiol Lett 328:174-81. 2012
    ..We propose to rename the toxin and the antitoxin as CptA and CptB (for Cytoskeleton Polymerization inhibiting Toxin), respectively...
  16. pmc Noncollagenous region of the streptococcal collagen-like protein is a trimerization domain that supports refolding of adjacent homologous and heterologous collagenous domains
    Zhuoxin Yu
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Protein Sci 19:775-85. 2010
    ..This information about their modular nature can be used in the production of novel types of bacterial collagen for biomaterial applications...
  17. ncbi request reprint MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli
    Yonglong Zhang
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Cell 12:913-23. 2003
    ..The role of such endoribonucleases may have broad implication in cell physiology under various growth conditions...
  18. pmc MqsR, a crucial regulator for quorum sensing and biofilm formation, is a GCU-specific mRNA interferase in Escherichia coli
    Yoshihiro Yamaguchi
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 284:28746-53. 2009
    ..MqsR forms a stable 2:1 complex with YgiT, and the complex likely functions as a repressor for the mqsR-ygiT operon by specifically binding to two different palindromic sequences present in the 5'-untranslated region of this operon...
  19. ncbi request reprint The HAMP linker in histidine kinase dimeric receptors is critical for symmetric transmembrane signal transduction
    Yan Zhu
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 279:48152-8. 2004
    ..This is the first demonstration that two HAMP linkers in a sensor dimer are functionally coupled for normal signal transduction; however, this functional coupling can be reduced when the HAMP linkers lose their symmetric nature...
  20. doi request reprint Applications of nucleic acid chaperone activity of CspA and its homologues
    Sangita Phadtare
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Mol Microbiol Biotechnol 17:110-7. 2009
    ..The low substrate specificity of Csps together with their compatibility with various enzymes and their stability and activity over a broad temperature range makes them ideal candidates to be used for a variety of processes...
  21. ncbi request reprint Bacterial toxin YafQ is an endoribonuclease that associates with the ribosome and blocks translation elongation through sequence-specific and frame-dependent mRNA cleavage
    Meredith H Prysak
    Department of Molecular Genetics, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Microbiol 71:1071-87. 2009
    ....
  22. pmc RNase activity of polynucleotide phosphorylase is critical at low temperature in Escherichia coli and is complemented by RNase II
    Naoki Awano
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Bacteriol 190:5924-33. 2008
    ....
  23. ncbi request reprint Interaction of EnvZ, a sensory histidine kinase, with phosphorylated OmpR, the cognate response regulator
    Takeshi Yoshida
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Microbiol 46:1283-94. 2002
    ..These findings support the hypothesis that osmotic signals regulate the level of the cellular concentration of OmpR-P by modulating the ratio of kinase to phosphatase activity of the bifunctional enzymatic activities of EnvZ...
  24. doi request reprint MazF, an mRNA interferase, mediates programmed cell death during multicellular Myxococcus development
    Hirofumi Nariya
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Cell 132:55-66. 2008
    ..Transcription of mrpC and mazF is negatively regulated via MrpC phosphorylation by a Ser/Thr kinase cascade. These findings reveal the regulated deployment of a toxin gene for developmental programmed cell death in bacteria...
  25. pmc Use of amino acids as inducers for high-level protein expression in the single-protein production system
    S Thangminlal Vaiphei
    Department of Biochemistry, Robert Wood Johnson Medical School, and Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854, USA
    Appl Environ Microbiol 76:6063-8. 2010
    ..In both systems, isotopic incorporation reached more than 98% labeling efficiency, significantly reducing the background attributable to the unlabeled target protein...
  26. pmc Genome-wide transcriptional analysis of the cold shock response in wild-type and cold-sensitive, quadruple-csp-deletion strains of Escherichia coli
    Sangita Phadtare
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Ln, Piscataway, NJ 08854, USA
    J Bacteriol 186:7007-14. 2004
    ..Relevance of these findings with respect to the known RNA chaperone function of CspA homologues is discussed...
  27. ncbi request reprint Solution NMR structure of ribosome-binding factor A (RbfA), a cold-shock adaptation protein from Escherichia coli
    Yuanpeng Janet Huang
    Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, NJ 08854, USA
    J Mol Biol 327:521-36. 2003
    ..coli operon. Structural and functional similarities between RbfA, NusA, and other bacterial type II KH domains suggest previously unsuspected evolutionary relationships between these cold-shock associated proteins...
  28. ncbi request reprint Characterization of the interactions within the mazEF addiction module of Escherichia coli
    Junjie Zhang
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 278:32300-6. 2003
    ..The present results demonstrate that MazE is composed of two domains, the N-terminal DNA-binding domain and the C-terminal domain interacting with MazF...
  29. ncbi request reprint CspB and CspL, thermostable cold-shock proteins from Thermotoga maritima
    Sangita Phadtare
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ, USA
    Genes Cells 8:801-10. 2003
    ..Csps help cells adapt to low-temperature growth through their RNA-binding and nucleic acid melting abilities, which lead to anti-termination of transcription...
  30. ncbi request reprint Cold-shock induced high-yield protein production in Escherichia coli
    Guoliang Qing
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Nat Biotechnol 22:877-82. 2004
    ..We have cloned 38 genes from a range of prokaryotic and eukaryotic organisms into both pCold and pET14 (ref. 3) systems, and found that pCold vectors are highly complementary to the widely used pET vectors...
  31. doi request reprint YeeU enhances the bundling of cytoskeletal polymers of MreB and FtsZ, antagonizing the CbtA (YeeV) toxicity in Escherichia coli
    Hisako Masuda
    Department of Biochemistry, Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School, Piscataway, NJ, USA
    Mol Microbiol 84:979-89. 2012
    ..Here we propose to rename YeeU as CbeA for cytoskeleton bundling-enhancing factor A...
  32. doi request reprint Segmental isotope labeling of proteins for NMR structural study using a protein S tag for higher expression and solubility
    Hiroshi Kobayashi
    Department of Biochemistry, Robert Wood Johnson Medical School, Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
    J Biomol NMR 52:303-13. 2012
    ..CS-Rosetta calculations also demonstrate that the core structure of full-length RbfA is similar to that of the RbfAΔ25 construct...
  33. ncbi request reprint Thermotoga maritima MazG protein has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities
    Junjie Zhang
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Biol Chem 278:21408-14. 2003
    ..These results demonstrated that Tm-MazG has dual enzymatic functions, NTPase and pyrophosphatase, and that these two enzymatic activities are coordinated...
  34. doi request reprint The E. coli single protein production system for production and structural analysis of membrane proteins
    Lili Mao
    Center for Advanced Technology and Medicine, UMDNJ Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA
    J Struct Funct Genomics 11:81-4. 2010
    ..Here we address some of the major bottlenecks in the structural studies of membrane proteins and discuss the ability of the new technology, the Single-Protein Production system, to help solve these bottlenecks...
  35. pmc Staphylococcus aureus MazF specifically cleaves a pentad sequence, UACAU, which is unusually abundant in the mRNA for pathogenic adhesive factor SraP
    Ling Zhu
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Bacteriol 191:3248-55. 2009
    ..aureus. This observation suggests a possible regulatory relationship between the MazEF(Sa) TA module and the pathogenicity in S. aureus...
  36. pmc Complementation analysis of the cold-sensitive phenotype of the Escherichia coli csdA deletion strain
    Naoki Awano
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Bacteriol 189:5808-15. 2007
    ..These results suggest that the primary role of CsdA in cold acclimation of cells is in mRNA decay and that its helicase activity is pivotal for promoting degradation of mRNAs stabilized at low temperature...
  37. ncbi request reprint Analysis of the role of the EnvZ linker region in signal transduction using a chimeric Tar/EnvZ receptor protein, Tez1
    Yan Zhu
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 278:22812-9. 2003
    ..In turn helix II propagates the resultant conformational signal into the downstream catalytic domain of EnvZ to regulate its bifunctional enzymatic activities...
  38. ncbi request reprint Autotomic behavior of the propeptide in propeptide-mediated folding of prosubtilisin E
    Liliana Falzon
    Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
    J Mol Biol 366:494-503. 2007
    ..This "autotomic" behavior of the propeptide may be a prevailing principle in propeptide-mediated protein folding...
  39. ncbi request reprint Probing catalytically essential domain orientation in histidine kinase EnvZ by targeted disulfide crosslinking
    Sheng jian Cai
    Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, NJ 08854, USA
    J Mol Biol 328:409-18. 2003
    ....
  40. pmc Interaction of an essential Escherichia coli GTPase, Der, with the 50S ribosome via the KH-like domain
    Jihwan Hwang
    Center for Advanced Biotechnology and Medicine, Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 679 Hoes Lane, Piscataway, NJ 08854, USA
    J Bacteriol 192:2277-83. 2010
    ..We also identified a region of Der that is critical for its specific interaction with 50S subunit...
  41. pmc The inhibitory mechanism of protein synthesis by YoeB, an Escherichia coli toxin
    Yonglong Zhang
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 284:6627-38. 2009
    ..As a result, the 3'-end portion of the mRNA was released from ribosomes, and translation initiation was effectively inhibited. These results demonstrate that YoeB primarily inhibits translation initiation...
  42. ncbi request reprint Three amino acids in Escherichia coli CspE surface-exposed aromatic patch are critical for nucleic acid melting activity leading to transcription antitermination and cold acclimation of cells
    Sangita Phadtare
    Department of Biochemistry Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 277:46706-11. 2002
    ....
  43. ncbi request reprint Formation of the stoichiometric complex of EnvZ, a histidine kinase, with its response regulator, OmpR
    Takeshi Yoshida
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Microbiol 46:1273-82. 2002
    ..On the basis of these results, a model is discussed to explain how cellular OmpR-P concentrations are regulated in response to medium osmolarity...
  44. ncbi request reprint Era and RbfA have overlapping function in ribosome biogenesis in Escherichia coli
    Koichi Inoue
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Mol Microbiol Biotechnol 11:41-52. 2006
    ..We propose that Era and RbfA have an overlapping function that is essential for ribosome biogenesis, and that RbfA becomes dispensable only at high temperatures because Era can complement its function only at higher temperatures...
  45. ncbi request reprint Single protein production in living cells facilitated by an mRNA interferase
    Motoo Suzuki
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
    Mol Cell 18:253-61. 2005
    ..This expression system enables unparalleled signal to noise ratios that should dramatically simplify structural and functional studies of previously intractable but biologically important proteins...
  46. ncbi request reprint Interference of mRNA function by sequence-specific endoribonuclease PemK
    Junjie Zhang
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Biol Chem 279:20678-84. 2004
    ..We propose that PemK and its homologues form a novel endoribonuclease family that interferes with mRNA function by cleaving cellular mRNAs in a sequence-specific manner...
  47. ncbi request reprint Transcription regulation of ompF and ompC by a single transcription factor, OmpR
    Takeshi Yoshida
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 281:17114-23. 2006
    ....
  48. ncbi request reprint Spontaneous subunit exchange and biochemical evidence for trans-autophosphorylation in a dimer of Escherichia coli histidine kinase (EnvZ)
    Sheng jian Cai
    Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, NJ 08854 5635, USA
    J Mol Biol 329:495-503. 2003
    ..Together, the present results provide biochemical evidence that EnvZ autophosphorylation occurs in trans and only within an EnvZ dimer...
  49. pmc Production of membrane proteins for NMR studies using the condensed single protein (cSPP) production system
    Lili Mao
    New York Center on Membrane Protein Structure, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA
    J Struct Funct Genomics 10:281-9. 2009
    ....
  50. pmc Significant enhanced expression and solubility of human proteins in Escherichia coli by fusion with protein S from Myxococcus xanthus
    Hiroshi Kobayashi
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Appl Environ Microbiol 75:5356-62. 2009
    ....
  51. doi request reprint The mRNA interferases, MazF-mt3 and MazF-mt7 from Mycobacterium tuberculosis target unique pentad sequences in single-stranded RNA
    Ling Zhu
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Mol Microbiol 69:559-69. 2008
    ..tuberculosis during infection...
  52. doi request reprint The use of the condensed single protein production system for isotope-labeled outer membrane proteins, OmpA and OmpX in E. coli
    S Thangminlal Vaiphei
    Department of Biochemistry, Robert Wood Johnson Medical School and Centre for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Biotechnol 47:205-10. 2011
    ..This method would be quite valuable for the study of pure proteins in their native membrane environment without the need of purification and reconstitution...
  53. ncbi request reprint Characterization of mRNA interferases from Mycobacterium tuberculosis
    Ling Zhu
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 281:18638-43. 2006
    ..These results indicate that M. tuberculosis contains sequence-specific mRNA interferases, which may play a role in the persistent dormancy of this devastating pathogen in human tissues...
  54. ncbi request reprint Insights into the mRNA cleavage mechanism by MazF, an mRNA interferase
    Yonglong Zhang
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 and Takara Bio Inc, Seta 3 4 1, Otsu, Shiga, 520 2193, Japan
    J Biol Chem 280:3143-50. 2005
    ..Therefore, MazF exhibits exquisite site specificity and has utility as an RNA-restriction enzyme for RNA structural studies or as an mRNA interferase to regulate cell growth in prokaryotic and eukaryotic cells...
  55. ncbi request reprint The mechanism of nucleic acid melting by a CspA family protein
    Sangita Phadtare
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Mol Biol 337:147-55. 2004
    ..The results allow us to orient a CspE molecule relative to the melting substrate and to put forward a mechanistic model of nucleic acid melting by Csps...
  56. ncbi request reprint Bacterial bioreactors for high yield production of recombinant protein
    Motoo Suzuki
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 281:37559-65. 2006
    ..Therefore, this expression system using Escherichia coli as a bioreactor is especially well suited to structural genomics, large-scale protein expressions, and the production of cytotoxic proteins...
  57. ncbi request reprint The role of RbfA in 16S rRNA processing and cell growth at low temperature in Escherichia coli
    Bing Xia
    Department of Biochemistry, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854 5635, USA
    J Mol Biol 332:575-84. 2003
    ....
  58. ncbi request reprint The role of the G2 box, a conserved motif in the histidine kinase superfamily, in modulating the function of EnvZ
    Yan Zhu
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Mol Microbiol 45:653-63. 2002
    ..The G2 box may modulate the interaction between these two domains in response to extracellular osmolarity...
  59. doi request reprint Use of E. coli for the production of a single protein
    Lili Mao
    Department of Biochemistry, Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School, Piscataway, NJ, USA
    Methods Mol Biol 899:177-85. 2012
    ..We have also demonstrated that the SPP system is a cost-effective protein production method for NMR structural studies because cell culture can be highly condensed without affecting protein yields...
  60. pmc Growth and translation inhibition through sequence-specific RNA binding by Mycobacterium tuberculosis VapC toxin
    Jared D Sharp
    Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry, New Jersey UMDNJ Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 287:12835-47. 2012
    ..Therefore, the activity of VapC-mt4 is mechanistically distinct from other TA toxins because it appears to primarily inhibit translation through selective, stable binding to RNA...
  61. pmc Escherichia coli RNase R has dual activities, helicase and RNase
    Naoki Awano
    Department of Biochemistry, Robert Wood Johnson Medical School, UMDNJ, CABM, 679 Hoes Lane, Piscataway, New Jersey 08854, USA
    J Bacteriol 192:1344-52. 2010
    ..We further demonstrated that RNase R exhibits helicase activity in vitro independent of its RNase activity. Our results shed light on the unique properties of RNase R and how it is distinct from other exoribonucleases in E. coli...
  62. pmc YeeV is an Escherichia coli toxin that inhibits cell division by targeting the cytoskeleton proteins, FtsZ and MreB
    Qian Tan
    Department of Biochemistry, Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854, USA
    Mol Microbiol 79:109-18. 2011
    ..The YeeV toxin is distinct from other well-studied toxins: it directs the binding of two cytoskeletal proteins and inhibits FtsZ and MreB simultaneously...
  63. pmc Significant bias against the ACA triplet in the tmRNA sequence of Escherichia coli K-12
    Sarah Baik
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Bacteriol 191:6157-66. 2009
    ..We propose that the unusual bias against ACA in tmRNA may have coevolved with the acquisition of MazF...
  64. pmc Expanding the family of collagen proteins: recombinant bacterial collagens of varying composition form triple-helices of similar stability
    Chunying Xu
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Piscataway, 08854, USA
    Biomacromolecules 11:348-56. 2010
    ..These collagen-like proteins provide a resource for the development of biomaterials with new properties...
  65. doi request reprint Solution NMR structure of the NlpC/P60 domain of lipoprotein Spr from Escherichia coli: structural evidence for a novel cysteine peptidase catalytic triad
    James M Aramini
    Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA
    Biochemistry 47:9715-7. 2008
    ....
  66. ncbi request reprint The nucleic acid melting activity of Escherichia coli CspE is critical for transcription antitermination and cold acclimation of cells
    Sangita Phadtare
    Department of Biochemistry, Robert Wood Johnson Medical School, Rutgers, State University of New Jersey, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Biol Chem 277:7239-45. 2002
    ..Thus, the nucleic acid-melting activity of Csp is critical for its prototypical function of supporting low temperature survival of the cell...
  67. ncbi request reprint The tandem GTPase, Der, is essential for the biogenesis of 50S ribosomal subunits in Escherichia coli
    Jihwan Hwang
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Mol Microbiol 61:1660-72. 2006
    ..We propose that Der plays a pivotal role in ribosome biogenesis possibly through interaction with rRNA or rRNA/r-protein complex...
  68. ncbi request reprint Cold shock response and adaptation at near-freezing temperature in microorganisms
    Masayori Inouye
    Robert Wood Johnson Medical School, Department of Biochemistry, 675 Hoes Lane, Piscataway, NJ 08854, USA
    Sci STKE 2004:pe26. 2004
    ..This emphasizes the fact that different groups of microorganisms exhibit distinct types of cold shock responses...
  69. pmc NBK/BIK antagonizes MCL-1 and BCL-XL and activates BAK-mediated apoptosis in response to protein synthesis inhibition
    Tsutomu Shimazu
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Genes Dev 21:929-41. 2007
    ..Although NBK/BIK is dispensable for development, it is the BH3-only protein targeted for inactivation by viruses, suggesting that it plays a role in pathogen/toxin response through apoptosis activation...
  70. ncbi request reprint The Cold Box stem-loop proximal to the 5'-end of the Escherichia coli cspA gene stabilizes its mRNA at low temperature
    Bing Xia
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 277:6005-11. 2002
    ....
  71. ncbi request reprint EnvZ-OmpR interaction and osmoregulation in Escherichia coli
    Sheng jian Cai
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 277:24155-61. 2002
    ..With His10-OmpR, we also determined that the K(d) value for the EnvZc-OmpR complex formation is 1.20 +/- 0.17 microm. On the basis of these results, the molecular mechanism of osmoregulation of ompF and ompC is discussed...
  72. ncbi request reprint Specific growth inhibition by acetate of an Escherichia coli strain expressing Era-dE, a dominant negative Era mutant
    Koichi Inoue
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Mol Microbiol Biotechnol 4:379-88. 2002
    ..We demonstrated that such cells with a high serine concentration became sensitive to acetate, however the reason for this acetate sensitivity is not known at the present...
  73. pmc DNA microarray analysis of the expression profile of Escherichia coli in response to treatment with 4,5-dihydroxy-2-cyclopenten-1-one
    Sangita Phadtare
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Bacteriol 184:6725-9. 2002
    ..Genes belonging to the regulon involved in synthesis of Cys are upregulated. In addition, rpoS and RpoS-regulated genes responding to various stresses and a number of genes responding to oxidative stress are upregulated...
  74. ncbi request reprint The role of tryptophan residues in the autoprocessing of prosubtilisin E
    Michio Sone
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Biochim Biophys Acta 1749:15-22. 2005
    ..These results demonstrate that both Trp106 and Trp113 play a specific role in propeptide processing and enzymatic activity, while Trp241 plays no considerable role on any of these activities...
  75. pmc MazG, a nucleoside triphosphate pyrophosphohydrolase, interacts with Era, an essential GTPase in Escherichia coli
    Junjie Zhang
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Bacteriol 184:5323-9. 2002
    ..Unlike mutT, a gene for another conserved nucleotide triphosphate pyrophosphohydrolase that functions as a mutator gene, the mazG deletion did not result in a mutator phenotype in E. coli...
  76. pmc Staphylococcus aureus YoeB homologues inhibit translation initiation
    Satoshi Yoshizumi
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Bacteriol 191:5868-72. 2009
    ..Here, we show that Staphylococcus aureus contains two YoeB homologues, both of which function as ribosome-dependent mRNA interferases to inhibit translation initiation in a manner identical to that of YoeB-ec from Escherichia coli...
  77. ncbi request reprint Mechanism of stabilization of a bacterial collagen triple helix in the absence of hydroxyproline
    Angela Mohs
    Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA
    J Biol Chem 282:29757-65. 2007
    ..The high enthalpic contribution to the stability of the Scl2 collagenous domain supports the presence of a hydration network in the absence of Hyp...
  78. ncbi request reprint Enhancement of the solubility of proteins overexpressed in Escherichia coli by heat shock
    Jingqiu Chen
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Mol Microbiol Biotechnol 4:519-24. 2002
    ..coli. Taken together, these results suggest that the "heat-shock protocol" is a generalizable and useful method for increasing the solubility of many proteins overexpressed in E. coli...
  79. pmc RelA functionally suppresses the growth defect caused by a mutation in the G domain of the essential Der protein
    Jihwan Hwang
    Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854, USA
    J Bacteriol 190:3236-43. 2008
    ..This is the first demonstration of suppression of impaired function of Der by a functional enzyme. A possible mechanism of the suppression of DerN321D by RelA overproduction is discussed...
  80. ncbi request reprint Structural and functional studies of the HAMP domain of EnvZ, an osmosensing transmembrane histidine kinase in Escherichia coli
    Ryuta Kishii
    Discovery Research Laboratories, Kyorin Pharmaceutical Co, Ltd, Shimotsuga, Tochigi 329 0114, Japan
    J Biol Chem 282:26401-8. 2007
    ..Berndt, F., Gruber, M., Linder, J. U., Truffault, V., Schultz, A., Martin, J., Schultz, J. E., Lupas, A. N., and Coles, M. (2006) Cell 126, 929-940)...
  81. ncbi request reprint Characterization of dual substrate binding sites in the homodimeric structure of Escherichia coli mRNA interferase MazF
    Guang Yao Li
    Division of Signaling Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ont, Canada M5G 2M9
    J Mol Biol 357:139-50. 2006
    ....
  82. ncbi request reprint Structural mechanism of transcriptional autorepression of the Escherichia coli RelB/RelE antitoxin/toxin module
    Guang Yao Li
    Division of Signaling Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
    J Mol Biol 380:107-19. 2008
    ....
  83. ncbi request reprint Human organic anion transporter hOAT1 forms homooligomers
    Mei Hong
    Department of Pharmaceutics, Rutgers, The State University of New Jersey, 08854, USA
    J Biol Chem 280:32285-90. 2005
    ..Taken together, this is the first study demonstrating that hOAT1 exists in the plasma membrane as a homooligomer, possibly trimer, and higher order of oligomer...
  84. pmc Structural characterization of Escherichia coli sensor histidine kinase EnvZ: the periplasmic C-terminal core domain is critical for homodimerization
    Ahmad Khorchid
    Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9
    Biochem J 385:255-64. 2005
    ....
  85. ncbi request reprint DNA immobilization and SAW response in ZnO nanotips grown on LiNbO3 substrates
    Zheng Zhang
    Department of Electrical and Computer Engineering, Rutgers University, Piscataway, NJ 08854 8058, USA
    IEEE Trans Ultrason Ferroelectr Freq Control 53:786-92. 2006
    ..This device structure possesses the advantages of both traditional SAW sensors and ZnO nanostructures...
  86. ncbi request reprint Enhancement of translation initiation by A/T-rich sequences downstream of the initiation codon in Escherichia coli
    Guoliang Qing
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ, USA
    J Mol Microbiol Biotechnol 6:133-44. 2003
    ..Collectively, the present data demonstrate that A/T richness of the region following the initiation codon plays a significant role in E. coli gene expression...
  87. ncbi request reprint Finding one of a kind: advances in single-protein production
    Liliana Falzon
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Curr Opin Biotechnol 17:347-52. 2006
    ..This technology not only provides a new high expression system for proteins, but also offers a novel avenue for protein structural studies...
  88. ncbi request reprint Single protein production (SPP) system in Escherichia coli
    Motoo Suzuki
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Nat Protoc 2:1802-10. 2007
    ..The procedure can be completed in 7 d after cloning of an ACA-less target gene into the expression system...

Research Grants32

  1. Cold Shock Vectors for Single Protein Production
    Masayori Inouye; Fiscal Year: 2004
    ..The proposed vector-host system will be highly useful not only for structural biology but also for medicinal research involving human proteins, which can not be obtained by conventional expression systems. ..
  2. STRESS RESPONSE AND ADAPTATION IN ESCHERICHIA COLI
    Masayori Inouye; Fiscal Year: 2002
    ..The proposed research will provide important insight into the fundamental principles of stress response and adaptation in prokaryotes. ..
  3. STRESS RESPONSE AND ADAPTATION IN ESCHERICHIA COLI
    Masayori Inouye; Fiscal Year: 2000
    ..The proposed research will provide important insight into the fundamental principles of stress response and adaptation in prokaryotes. ..
  4. Propeptide-Mediated Protein Folding
    Masayori Inouye; Fiscal Year: 2006
    ..Another emphasis is to investigate how the nascent folding intermediate can be activated with small peptide substrates. ..
  5. REVERSE TRANSCRIPTASES IN THE PROKARYOTES
    Masayori Inouye; Fiscal Year: 2004
    ....
  6. Propeptide-Mediated Protein Folding
    Masayori Inouye; Fiscal Year: 2004
    ..Another emphasis is to investigate how the nascent folding intermediate can be activated with small peptide substrates. ..
  7. Propeptide-Mediated Protein Folding
    Masayori Inouye; Fiscal Year: 2005
    ..Another emphasis is to investigate how the nascent folding intermediate can be activated with small peptide substrates. ..
  8. Propeptide-Mediated Protein Folding
    Masayori Inouye; Fiscal Year: 2005
    ..Another emphasis is to investigate how the nascent folding intermediate can be activated with small peptide substrates. ..
  9. Signal Transduction by Histidine Kinases and their Response Regulators
    Masayori Inouye; Fiscal Year: 2007
    ..We will also analyze the reciprocal regulatory mechanism for the ompF and ompC outer membrane porin genes at the level of transcription by OmpR-P and at the level of translation by two antisense RNAs, micF and micC RNAs by RT-PCR. ..
  10. Signal Transduction by Histidine Kinases and their Response Regulators
    Masayori Inouye; Fiscal Year: 2007
    ..abstract_text> ..
  11. Deciphering of the Toxin-Antitoxin Systems in E. coli
    Masayori Inouye; Fiscal Year: 2009
    ....
  12. The Method for Determination of Membrane Protein Structures without Purification
    Masayori Inouye; Fiscal Year: 2009
    ..This will, no doubt, revolutionize the structural biology of membrane proteins and will make a major contribution to our understanding of how membrane proteins function in living cells. ..
  13. The Method for Determination of Membrane Protein Structures without Purification
    Masayori Inouye; Fiscal Year: 2010
    ..This will, no doubt, revolutionize the structural biology of membrane proteins and will make a major contribution to our understanding of how membrane proteins function in living cells. ..
  14. STRESS RESPONSE AND ADAPTATION IN ESCHERICHIA COLI
    Masayori Inouye; Fiscal Year: 2003
    ..The proposed research will provide important insight into the fundamental principles of stress response and adaptation in prokaryotes. ..
  15. REVERSE TRANSCRIPTASES IN THE PROKARYOTES
    Masayori Inouye; Fiscal Year: 2001
    ....
  16. REVERSE TRANSCRIPTASES IN THE PROKARYOTES
    Masayori Inouye; Fiscal Year: 2002
    ....
  17. PROPEPTIDE MEDIATED PROTEIN FOLDING
    Masayori Inouye; Fiscal Year: 1999
    ..We will characterize these conformers in terms of enzymatic specificity, thermostability, and secondary and tertiary structures. ..
  18. REVERSE TRANSCRIPTASES IN THE PROKARYOTES
    Masayori Inouye; Fiscal Year: 2003
    ....
  19. Deciphering of the Toxin-Antitoxin Systems in E. coli
    Masayori Inouye; Fiscal Year: 2010
    ....
  20. Signal Transduction by Histidine Kinases and their Response Regulators
    Masayori Inouye; Fiscal Year: 2009
    ..abstract_text> ..
  21. PROPEPTIDE MEDIATED PROTEIN FOLDING
    Masayori Inouye; Fiscal Year: 2000
    ..We will characterize these conformers in terms of enzymatic specificity, thermostability, and secondary and tertiary structures. ..
  22. STRESS RESPONSE AND ADAPTATION IN ESCHERICHIA COLI
    Masayori Inouye; Fiscal Year: 2001
    ..The proposed research will provide important insight into the fundamental principles of stress response and adaptation in prokaryotes. ..
  23. PROPEPTIDE MEDIATED PROTEIN FOLDING
    Masayori Inouye; Fiscal Year: 2001
    ..We will characterize these conformers in terms of enzymatic specificity, thermostability, and secondary and tertiary structures. ..
  24. Signal Transduction by Histidine Kinases and their Response Regulators
    Masayori Inouye; Fiscal Year: 2006
    ..We will also analyze the reciprocal regulatory mechanism for the ompF and ompC outer membrane porin genes at the level of transcription by OmpR-P and at the level of translation by two antisense RNAs, micF and micC RNAs by RT-PCR. ..
  25. Propeptide-Mediated Protein Folding
    Masayori Inouye; Fiscal Year: 2003
    ..Another emphasis is to investigate how the nascent folding intermediate can be activated with small peptide substrates. ..
  26. Deciphering of the Toxin-Antitoxin Systems in E. coli
    Masayori Inouye; Fiscal Year: 2010
    ....