Inca Ghosh

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. doi request reprint Site-specific protein labeling by intein-mediated protein ligation
    Inca Ghosh
    New England Biolabs, Ipswich, MA 01938, USA
    Methods Mol Biol 705:87-107. 2011
  2. ncbi request reprint Intein-mediated peptide arrays for epitope mapping and kinase/phosphatase assays
    Ming Qun Xu
    New England Biolabs, Beverly, MA, USA
    Methods Mol Biol 381:313-38. 2007
  3. ncbi request reprint Design, preparation and use of ligated phosphoproteins: a novel approach to study protein phosphatases by dot blot array, ELISA and Western blot assays
    Luo Sun
    New England Biolabs, Inc Ipswich, MA 01938 2723, USA
    Methods 42:220-6. 2007
  4. doi request reprint Fluorescent site-specific labeling of Escherichia coli expressed proteins with Sfp phosphopantetheinyl transferase
    Aihua Zhang
    New England Biolabs, 01938 Ipswich, MA, USA
    Methods Mol Biol 705:295-307. 2011
  5. ncbi request reprint Producing peptide arrays for epitope mapping by intein-mediated protein ligation
    Luo Sun
    New England Biolabs, Beverly, MA 01915, USA
    Biotechniques 37:430-6, 438, 440 passim. 2004
  6. ncbi request reprint An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays
    Inca Ghosh
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    J Immunol Methods 293:85-95. 2004
  7. ncbi request reprint Use of intein-mediated phosphoprotein arrays to study substrate specificity of protein phosphatases
    Samvel Kochinyan
    New England Biolabs, Inc, Ipswich, MA 01938 2723, USA
    Biotechniques 42:63-9. 2007
  8. ncbi request reprint Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein
    Jie Xu
    New England Biolabs, Beverly, MA, USA
    Biotechniques 36:976-8, 980-1. 2004
  9. ncbi request reprint Generation of an affinity column for antibody purification by intein-mediated protein ligation
    Luo Sun
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    J Immunol Methods 282:45-52. 2003

Collaborators

Detail Information

Publications9

  1. doi request reprint Site-specific protein labeling by intein-mediated protein ligation
    Inca Ghosh
    New England Biolabs, Ipswich, MA 01938, USA
    Methods Mol Biol 705:87-107. 2011
    ..This chapter also gives a general review of the critical parameters of intein-mediated cleavage and ligation reactions...
  2. ncbi request reprint Intein-mediated peptide arrays for epitope mapping and kinase/phosphatase assays
    Ming Qun Xu
    New England Biolabs, Beverly, MA, USA
    Methods Mol Biol 381:313-38. 2007
    ..In this report, several experimental procedures including epitope mapping and the study of protein modifications were described...
  3. ncbi request reprint Design, preparation and use of ligated phosphoproteins: a novel approach to study protein phosphatases by dot blot array, ELISA and Western blot assays
    Luo Sun
    New England Biolabs, Inc Ipswich, MA 01938 2723, USA
    Methods 42:220-6. 2007
    ..This report describes detailed experimental procedures for preparation of LPP substrates and their use in PP assays based on immobilization on solid supports...
  4. doi request reprint Fluorescent site-specific labeling of Escherichia coli expressed proteins with Sfp phosphopantetheinyl transferase
    Aihua Zhang
    New England Biolabs, 01938 Ipswich, MA, USA
    Methods Mol Biol 705:295-307. 2011
    ..The fluorescently labeled E14.7 fusion protein was analyzed with a fluorescence imager and subsequently transfected into mammalian cells for imaging with a fluorescence microscope...
  5. ncbi request reprint Producing peptide arrays for epitope mapping by intein-mediated protein ligation
    Luo Sun
    New England Biolabs, Beverly, MA 01915, USA
    Biotechniques 37:430-6, 438, 440 passim. 2004
    ..This novel approach allows for synthetic peptides to be uniformly arrayed onto membranes, compatible with a variety of applications...
  6. ncbi request reprint An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays
    Inca Ghosh
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    J Immunol Methods 293:85-95. 2004
    ..These techniques do not require sophisticated equipment, reagents, or skills thereby providing a simple method for research and development...
  7. ncbi request reprint Use of intein-mediated phosphoprotein arrays to study substrate specificity of protein phosphatases
    Samvel Kochinyan
    New England Biolabs, Inc, Ipswich, MA 01938 2723, USA
    Biotechniques 42:63-9. 2007
    ..The IPL technique overcomes the disadvantages of current methods and is a versatile system for the facile production of protein substrates containing well-defined structural motifs for the study of protein modification enzymes...
  8. ncbi request reprint Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein
    Jie Xu
    New England Biolabs, Beverly, MA, USA
    Biotechniques 36:976-8, 980-1. 2004
    ..We demonstrate the effectiveness of this approach by mutational analysis of peptide substrates derived from human cyclin-dependent kinase, Cdc2, which contains a phosphorylation site of human c-Src protein tyrosine kinase...
  9. ncbi request reprint Generation of an affinity column for antibody purification by intein-mediated protein ligation
    Luo Sun
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    J Immunol Methods 282:45-52. 2003
    ..Peptide epitopes possessing an N-terminal cysteine were ligated to the chitin bound CBD tag. We demonstrate that the resulting peptide columns permit the highly specific and efficient affinity purification of antibodies from animal sera...