Thomas C Evans

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. ncbi request reprint Protein splicing elements and plants: from transgene containment to protein purification
    Thomas C Evans
    New England Biolabs, Inc, Beverly, Massachusetts 01915, USA
    Annu Rev Plant Biol 56:375-92. 2005
  2. pmc A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase
    Jerome Mauris
    New England Biolabs Inc, Ipswich, Massachusetts 01938, USA
    J Biol Chem 285:11087-92. 2010
  3. pmc Adenosine triphosphate stimulates Aquifex aeolicus MutL endonuclease activity
    Jerome Mauris
    New England Biolabs, Inc, Ipswich, Massachusetts, USA
    PLoS ONE 4:e7175. 2009
  4. pmc DNA damage in preserved specimens and tissue samples: a molecular assessment
    Juergen Zimmermann
    New England Biolabs Inc, 240 County Rd, Ipswich, MA, 01938, USA
    Front Zool 5:18. 2008
  5. ncbi request reprint Mutational analysis of protein splicing, cleavage, and self-association reactions mediated by the naturally split Ssp DnaE intein
    Nicole Magnasco Nichols
    New England Biolabs, Inc, Beverly, Massachusetts 01915 5599, USA
    Biochemistry 43:10265-76. 2004
  6. ncbi request reprint Zinc ion effects on individual Ssp DnaE intein splicing steps: regulating pathway progression
    Nicole Magnasco Nichols
    New England Biolabs, Inc, Beverly, Massachusetts 01915 5599, USA
    Biochemistry 42:5301-11. 2003
  7. pmc Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes
    Hang Gyeong Chin
    New England Biolabs, 32 Tozer Road, Beverly, MA 01915 5054, USA
    Proc Natl Acad Sci U S A 100:4510-5. 2003
  8. doi request reprint Efficient DNA ligation in DNA-RNA hybrid helices by Chlorella virus DNA ligase
    Gregory J S Lohman
    DNA Enzymes Division, New England Biolabs, Inc, Ipswich, MA 01938 2723, USA, RNA Biology Division, New England Biolabs, Inc, Ipswich, MA 01938 2723, USA and Applications Development, New England Biolabs, Inc, Ipswich, MA 01938 2723, USA
    Nucleic Acids Res 42:1831-44. 2014
  9. pmc Kinetic characterization of single strand break ligation in duplex DNA by T4 DNA ligase
    Gregory J S Lohman
    New England Biolabs Inc, Ipswich, Massachusetts 01938 2723, USA
    J Biol Chem 286:44187-96. 2011
  10. doi request reprint Site-specific protein labeling by intein-mediated protein ligation
    Inca Ghosh
    New England Biolabs, Ipswich, MA 01938, USA
    Methods Mol Biol 705:87-107. 2011

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Protein splicing elements and plants: from transgene containment to protein purification
    Thomas C Evans
    New England Biolabs, Inc, Beverly, Massachusetts 01915, USA
    Annu Rev Plant Biol 56:375-92. 2005
    ..Finally, relevant techniques that may be used in plants in the future are discussed...
  2. pmc A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase
    Jerome Mauris
    New England Biolabs Inc, Ipswich, Massachusetts 01938, USA
    J Biol Chem 285:11087-92. 2010
    ....
  3. pmc Adenosine triphosphate stimulates Aquifex aeolicus MutL endonuclease activity
    Jerome Mauris
    New England Biolabs, Inc, Ipswich, Massachusetts, USA
    PLoS ONE 4:e7175. 2009
    ..Mutational analysis has identified the DQHA(X)(2)E(X)(4)E motif present in the C-terminus of PMS2 homologues as important for endonuclease activity...
  4. pmc DNA damage in preserved specimens and tissue samples: a molecular assessment
    Juergen Zimmermann
    New England Biolabs Inc, 240 County Rd, Ipswich, MA, 01938, USA
    Front Zool 5:18. 2008
    ..We characterize the fragmentation state of the DNA and aim to identify abundant nucleoside lesions. Finally, simple models are introduced to describe the DNA fragmentation based on nicks and double-strand breaks...
  5. ncbi request reprint Mutational analysis of protein splicing, cleavage, and self-association reactions mediated by the naturally split Ssp DnaE intein
    Nicole Magnasco Nichols
    New England Biolabs, Inc, Beverly, Massachusetts 01915 5599, USA
    Biochemistry 43:10265-76. 2004
    ....
  6. ncbi request reprint Zinc ion effects on individual Ssp DnaE intein splicing steps: regulating pathway progression
    Nicole Magnasco Nichols
    New England Biolabs, Inc, Beverly, Massachusetts 01915 5599, USA
    Biochemistry 42:5301-11. 2003
    ..By inhibiting both the splicing pathway and spontaneous hydrolysis with Zn(2+), reactants can be diverted from the trans splicing to the trans cleavage pathway where DTT and EDTA can regulate N- and C-terminal cleavage, respectively...
  7. pmc Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes
    Hang Gyeong Chin
    New England Biolabs, 32 Tozer Road, Beverly, MA 01915 5054, USA
    Proc Natl Acad Sci U S A 100:4510-5. 2003
    ..Furthermore, these transgenic plants displayed improved resistance to the herbicide N-(phosphonomethyl)glycine (glyphosate) when compared with wild-type N. tabacum...
  8. doi request reprint Efficient DNA ligation in DNA-RNA hybrid helices by Chlorella virus DNA ligase
    Gregory J S Lohman
    DNA Enzymes Division, New England Biolabs, Inc, Ipswich, MA 01938 2723, USA, RNA Biology Division, New England Biolabs, Inc, Ipswich, MA 01938 2723, USA and Applications Development, New England Biolabs, Inc, Ipswich, MA 01938 2723, USA
    Nucleic Acids Res 42:1831-44. 2014
    ..Furthermore, this ligase was used in a ligation-based detection assay system to show increased sensitivity over T4 DNA ligase in the specific detection of a target mRNA...
  9. pmc Kinetic characterization of single strand break ligation in duplex DNA by T4 DNA ligase
    Gregory J S Lohman
    New England Biolabs Inc, Ipswich, Massachusetts 01938 2723, USA
    J Biol Chem 286:44187-96. 2011
    ..Pre-steady state reactions displayed a clear burst phase, consistent with this conclusion...
  10. doi request reprint Site-specific protein labeling by intein-mediated protein ligation
    Inca Ghosh
    New England Biolabs, Ipswich, MA 01938, USA
    Methods Mol Biol 705:87-107. 2011
    ..This chapter also gives a general review of the critical parameters of intein-mediated cleavage and ligation reactions...
  11. ncbi request reprint Sequence specificity and role of proximal amino acids of the histone H3 tail on catalysis of murine G9A lysine 9 histone H3 methyltransferase
    Hang Gyeong Chin
    New England Biolabs, 240 County Road, Ipswich, Massachusetts 01938 2723, USA
    Biochemistry 44:12998-3006. 2005
    ..Thus, Ser-10/Thr-11 phosphorylation impairs Lys-9 methylation. These data suggest that the sequence context of the modified residue affects G9a activity and the modification in the proximal amino acids influences methylation...
  12. ncbi request reprint Recent advances in protein splicing: manipulating proteins in vitro and in vivo
    Ming Qun Xu
    New England Biolabs, Inc, 32 Tozer Road, Beverly, Massachusetts 01915, USA
    Curr Opin Biotechnol 16:440-6. 2005
    ..Inteins have been utilized in the production of protein and peptide arrays, as molecular switches and in the reconstitution of functional proteins by split-gene techniques...
  13. pmc Diagnosis of brugian filariasis by loop-mediated isothermal amplification
    Catherine B Poole
    New England Biolabs, Ipswich, Massachusetts, United States of America
    PLoS Negl Trop Dis 6:e1948. 2012
    ..The results indicate that the Brugia Hha I repeat LAMP assay is rapid, sensitive and Brugia-specific with the potential to be developed further as a field tool for diagnosis and mapping of brugian filariasis...
  14. doi request reprint Simultaneous multiple target detection in real-time loop-mediated isothermal amplification
    Nathan A Tanner
    New England Biolabs, Ipswich, MA, USA
    Biotechniques 53:81-9. 2012
    ..The coupling of this new multiplex technique with next generation isothermal DNA polymerases should increase the utility of the LAMP method for molecular diagnostics...
  15. ncbi request reprint Purification of recombinant proteins from E. coli by engineered inteins
    Ming Qun Xu
    New England Biolabs, Inc, Beverly, MA, USA
    Methods Mol Biol 205:43-68. 2003
  16. ncbi request reprint An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays
    Inca Ghosh
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    J Immunol Methods 293:85-95. 2004
    ..These techniques do not require sophisticated equipment, reagents, or skills thereby providing a simple method for research and development...
  17. doi request reprint Loop-mediated isothermal amplification for detection of nucleic acids
    Nathan A Tanner
    New England Biolabs, Ipswich, Massachusetts
    Curr Protoc Mol Biol 105:Unit 15.14.. 2014
    ..Basic LAMP protocols are provided herein for detection of specific DNA and RNA targets, along with a method to perform multiplex LAMP reactions, permitting even greater flexibility from this powerful technique...
  18. ncbi request reprint Crystal structures of an intein from the split dnaE gene of Synechocystis sp. PCC6803 reveal the catalytic model without the penultimate histidine and the mechanism of zinc ion inhibition of protein splicing
    Ping Sun
    Laboratory of Structural Biology, Tsinghua University, Beijing 100084, People s Republic of China
    J Mol Biol 353:1093-105. 2005
    ....