MICHAEL LORAN DUSTIN

Summary

Affiliation: New York University
Country: USA

Publications

  1. ncbi request reprint Opposing effects of PKCtheta and WASp on symmetry breaking and relocation of the immunological synapse
    Tasha N Sims
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Cell 129:773-85. 2007
  2. pmc Signaling at neuro/immune synapses
    Michael L Dustin
    The Helen L and Martin S Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Clin Invest 122:1149-55. 2012
  3. pmc The cellular context of T cell signaling
    Michael L Dustin
    Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Immunity 30:482-92. 2009
  4. doi request reprint Insights into function of the immunological synapse from studies with supported planar bilayers
    Michael L Dustin
    Helen L and Martin S Kimmel Center for Biology and Medicine in the Skirball Institute for Biomolecular Medicine and Department of Pathology, NYU School of Medicine, New York, NY 10016, USA
    Curr Top Microbiol Immunol 340:1-24. 2010
  5. doi request reprint Visualizing immune system complexity
    Michael L Dustin
    Division of Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, New York University, New York, NY 10016, USA
    Sci Signal 2:mr4. 2009
  6. pmc Modular design of immunological synapses and kinapses
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute for Biomolecular Medicine, The Helen L and Martin S Kimmel Center for Biology and Medicine, New York University School of Medicine, New York 10016, USA
    Cold Spring Harb Perspect Biol 1:a002873. 2009
  7. pmc Supported bilayers at the vanguard of immune cell activation studies
    Michael L Dustin
    Helen L and Martin S Kimmel Center for Biology and Medicine in the Skirball Institute for Biomolecular Medicine and Department of Pathology, NYU School of Medicine, New York, 10016, USA
    J Struct Biol 168:152-60. 2009
  8. pmc Cytotoxic immunological synapses
    Michael L Dustin
    Helen, Martin Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Immunol Rev 235:24-34. 2010
  9. doi request reprint New insights into the T cell synapse from single molecule techniques
    Michael L Dustin
    Helene and Martin Kimmel Center for Biology and Medicine of the Skirball Institute of Biomolecular Medicine, Department of Pathology, New York University School of Medicine, 540 First Avenue, New York, New York 10012, USA
    Nat Rev Immunol 11:672-84. 2011
  10. ncbi request reprint Visualizing dendritic cell networks in vivo
    Randall L Lindquist
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA
    Nat Immunol 5:1243-50. 2004

Research Grants

  1. REQUIREMENT FOR SENSITIVE T CELL RESPONSE TO ANTIGEN
    Michael Dustin; Fiscal Year: 2005
  2. PHYSIOLOGICAL CHEMISTRY OF INTEGRIN FUNCTION
    Michael Dustin; Fiscal Year: 2007
  3. Environmental Control of the Immunological Synapse
    Michael Dustin; Fiscal Year: 2007
  4. PHYSIOLOGICAL CHEMISTRY OF INTEGRIN FUNCTION
    Michael Dustin; Fiscal Year: 2002
  5. Environmental Control of the Immunological Synapse
    MICHAEL LORAN DUSTIN; Fiscal Year: 2010

Collaborators

Detail Information

Publications64

  1. ncbi request reprint Opposing effects of PKCtheta and WASp on symmetry breaking and relocation of the immunological synapse
    Tasha N Sims
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Cell 129:773-85. 2007
    ..WASp(-/-) T cells displayed normal IS formation but were unable to reform IS after migration unless PKCtheta was inhibited. Thus, opposing effects of PKCtheta and WASp control IS stability through pSMAC symmetry breaking and reformation...
  2. pmc Signaling at neuro/immune synapses
    Michael L Dustin
    The Helen L and Martin S Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Clin Invest 122:1149-55. 2012
    ..A better understanding of the shared mechanisms between immunological and neural synapses could aid in the development of new therapeutic modalities for immunological, neurological, and neuroimmunological disorders alike...
  3. pmc The cellular context of T cell signaling
    Michael L Dustin
    Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Immunity 30:482-92. 2009
    ..This review discusses how these concepts emerged from early studies on adhesion, signaling, and cell biology of T cells...
  4. doi request reprint Insights into function of the immunological synapse from studies with supported planar bilayers
    Michael L Dustin
    Helen L and Martin S Kimmel Center for Biology and Medicine in the Skirball Institute for Biomolecular Medicine and Department of Pathology, NYU School of Medicine, New York, NY 10016, USA
    Curr Top Microbiol Immunol 340:1-24. 2010
    ..A major goal for the field is determining quantitative rules involved in signaling complex formation by innate and adaptive receptor systems...
  5. doi request reprint Visualizing immune system complexity
    Michael L Dustin
    Division of Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, New York University, New York, NY 10016, USA
    Sci Signal 2:mr4. 2009
    ....
  6. pmc Modular design of immunological synapses and kinapses
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute for Biomolecular Medicine, The Helen L and Martin S Kimmel Center for Biology and Medicine, New York University School of Medicine, New York 10016, USA
    Cold Spring Harb Perspect Biol 1:a002873. 2009
    ..The T lymphocyte actively assembles the immunological synapse pattern following a modular design with roots in actin-myosin-based motility...
  7. pmc Supported bilayers at the vanguard of immune cell activation studies
    Michael L Dustin
    Helen L and Martin S Kimmel Center for Biology and Medicine in the Skirball Institute for Biomolecular Medicine and Department of Pathology, NYU School of Medicine, New York, 10016, USA
    J Struct Biol 168:152-60. 2009
    ..A major goal for the field is determining quantitative rules involved in signaling complex formation...
  8. pmc Cytotoxic immunological synapses
    Michael L Dustin
    Helen, Martin Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Immunol Rev 235:24-34. 2010
    ..This review provides an overview of work on cytotoxic immunological synapses and suggests pathways forward in applying this information to the development of therapeutic agents...
  9. doi request reprint New insights into the T cell synapse from single molecule techniques
    Michael L Dustin
    Helene and Martin Kimmel Center for Biology and Medicine of the Skirball Institute of Biomolecular Medicine, Department of Pathology, New York University School of Medicine, 540 First Avenue, New York, New York 10012, USA
    Nat Rev Immunol 11:672-84. 2011
    ..This is an exciting time in T cell biology, and further innovation in imaging and genomics is likely to lead to a greater understanding of how T cells are activated...
  10. ncbi request reprint Visualizing dendritic cell networks in vivo
    Randall L Lindquist
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA
    Nat Immunol 5:1243-50. 2004
    ..Mature DCs were more motile than steady-state DCs and were rapidly dispersed and integrated into the sessile network, facilitating their interaction with migrating T cells...
  11. pmc Stable T cell-dendritic cell interactions precede the development of both tolerance and immunity in vivo
    Guy Shakhar
    Program in Molecular Pathogenesis and Department of Pathology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
    Nat Immunol 6:707-14. 2005
    ..Thus, early antigen-dependent T cell arrest on DCs is a shared feature of tolerance and priming associated with activation and proliferation...
  12. ncbi request reprint T-cell activation: a multidimensional signaling network
    Su Yi Tseng
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Curr Opin Cell Biol 14:575-80. 2002
    ..This stable junction interrupts T cell migration, and provides a platform for temporally regulated co-stimulatory receptor signaling spanning a period of days...
  13. pmc CD80 cytoplasmic domain controls localization of CD28, CTLA-4, and protein kinase Ctheta in the immunological synapse
    Su Yi Tseng
    New York University School of Medicine, Skirball Institute, New York, NY 10016, USA
    J Immunol 175:7829-36. 2005
    ..Thus, the cytoplasmic tail of CD80 regulates its spatial localization at the IS and that of its receptors and T cell signaling molecules such as protein kinase Ctheta, and thereby facilitates full T cell activation...
  14. ncbi request reprint Regulation of T cell migration through formation of immunological synapses: the stop signal hypothesis
    Michael L Dustin
    Program in Molecular Pathogenesis and Department of Pathology, Skirball Institute of Molecular Medicine, New York University School of Medicine, NY 10016, USA
    Adv Exp Med Biol 512:191-201. 2002
  15. ncbi request reprint The immunological synapse: integrins take the stage
    Tasha N Sims
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Immunol Rev 186:100-17. 2002
    ..Each major signal transduction pathway has branches leading to the nucleus and others that feed back on cytoskeletal and membrane regulation at the IS...
  16. ncbi request reprint Peptide-MHC potency governs dynamic interactions between T cells and dendritic cells in lymph nodes
    Dimitris Skokos
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA
    Nat Immunol 8:835-44. 2007
    ..The pMHC complexes of lower potency instead induced T cell anergy by a biochemically distinct process that did not affect T cell dynamics...
  17. pmc Phospholipase D1 regulates lymphocyte adhesion via upregulation of Rap1 at the plasma membrane
    Adam Mor
    The Cancer Institute, NYU School of Medicine, New York, New York 10016, USA
    Mol Cell Biol 29:3297-306. 2009
    ..Our data support a model whereby PLD1 regulates Rap1 activity by controlling exocytosis of a stored, vesicular pool of Rap1 that can be activated by C3G upon delivery to the plasma membrane...
  18. pmc Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse
    Oren Milstein
    Programs in Molecular Pathogenesis and Structural Biology, Helen and Martin Kimmel Center for Biology and Medicine of the Skirball Institute and New York University School of Medicine, New York, New York 10016, USA
    J Biol Chem 283:34414-22. 2008
    ..We propose that this reorganization of the immunological synapse sequesters the T cell antigen receptor in a location where it cannot interact with its ligand and dramatically reduces T cell sensitivity...
  19. pmc T cell-dendritic cell immunological synapses contain TCR-dependent CD28-CD80 clusters that recruit protein kinase C theta
    Su Yi Tseng
    Program in Molecular Pathogenesis, Helen L and Martin S Kimmel Center for Biology and Medicine of the Skirball Institute of Biomolecular Medicine and Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    J Immunol 181:4852-63. 2008
    ..Thus, the T cell-DC interface contains dynamic costimulatory foci that share characteristics of microclusters and cSMACs...
  20. pmc Micropatterning of costimulatory ligands enhances CD4+ T cell function
    Keyue Shen
    Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
    Proc Natl Acad Sci U S A 105:7791-6. 2008
    ..With these patterns, we show that the peripheral presentation of CD28 has a larger impact on IL-2 secretion than CD3 colocalization/segregation...
  21. ncbi request reprint The lymphocyte function-associated antigen-1 receptor costimulates plasma membrane Ras via phospholipase D2
    Adam Mor
    Department of Medicine, NYU School of Medicine, New York, NY 10016, USA
    Nat Cell Biol 9:713-9. 2007
    ..PLD2 and phosphatidic acid phosphatase (PAP) were required for Ras activation on the plasma membrane. Thus, LFA-1 acts through PLD2 to reshape the pattern of Ras activation downstream of the TCR...
  22. pmc Regulatory T cells inhibit stable contacts between CD4+ T cells and dendritic cells in vivo
    Carlos E Tadokoro
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, and Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    J Exp Med 203:505-11. 2006
    ..Thus, T reg cells can exert an early effect on immune responses by attenuating the establishment of stable contacts during priming of naive T cells by DCs...
  23. pmc Actin and agonist MHC-peptide complex-dependent T cell receptor microclusters as scaffolds for signaling
    Gabriele Campi
    Department of Pathology and the Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, New York, NY 10016, USA
    J Exp Med 202:1031-6. 2005
    ..We propose that Src kinase-independent formation of TCR microclusters in response to agonist MHC-peptide provides an actin-dependent scaffold for signal amplification...
  24. doi request reprint Synaptic asymmetry to go
    Michael L Dustin
    Helen L and Martin S Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Cell 132:733-4. 2008
    ..In this issue of Cell, Yeh et al. (2008) now reveal a direct link between T cell polarity and the production of proinflammatory cytokines in mice lacking the class I MHC-restricted T cell-associated molecule (Crtam)...
  25. pmc Functional anatomy of T cell activation and synapse formation
    David R Fooksman
    Department of Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, NYU School of Medicine, New York, 10016, USA
    Annu Rev Immunol 28:79-105. 2010
    ..In this review, we examine the molecular components, geometry, and timing underlying kinapses and synapses. We integrate recent molecular and physiological data to provide a synthesis and suggest ways forward...
  26. ncbi request reprint Supported planar bilayers for study of the immunological synapse
    Michael L Dustin
    Skirball Institute of Biomolecular Medicine, New York, New York, USA
    Curr Protoc Immunol . 2007
    ..This provides a more physiological presentation of cell-surface molecules and supports visualization of protein rearrangement on the bilayer by live cells...
  27. pmc Development and migration of plasma cells in the mouse lymph node
    David R Fooksman
    Program in Molecular Pathogenesis and Department of Pathology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Immunity 33:118-27. 2010
    ..Taken together, we suggest pre-PCs undergo a persistent random walk to find the medullary cords, where localized chemokines help retain these cells until they undergo differentiation and arrest in situ...
  28. pmc In vivo imaging approaches in animal models of rheumatoid arthritis
    Michael L Dustin
    Skirball Institute of Biomolecular Medicine and Department of Pathology, New York University School of Medicine, New York, USA
    Arthritis Res Ther 5:165-71. 2003
    ..These approaches will probably shed light on the specific local mechanisms involved in chronic inflammation and provide real time monitoring approaches to follow cellular and molecular events related to disease development...
  29. pmc Two-photon laser scanning microscopy imaging of intact spinal cord and cerebral cortex reveals requirement for CXCR6 and neuroinflammation in immune cell infiltration of cortical injury sites
    Jiyun V Kim
    Helen L and Martin S Kimmel Center for Biology and Medicine of the Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    J Immunol Methods 352:89-100. 2010
    ..Interestingly, infiltration of the cerebral cortex by GFP(+) cells in these mice required three conditions: EAE induction, cortical injury and expression of CXCR6 on immune cells...
  30. doi request reprint Hunter to gatherer and back: immunological synapses and kinapses as variations on the theme of amoeboid locomotion
    Michael L Dustin
    Helen L and Martin S Kimmel Center for Biology and Medicine of the Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
    Annu Rev Cell Dev Biol 24:577-96. 2008
    ..Crtam is involved in postsynaptic polarity in early kinapses prior to cell division. It is unlikely that the immune system is unique in using symmetrization to stop migration without inactivating motile machinery...
  31. pmc The class II phosphatidylinositol 3 kinase C2beta is required for the activation of the K+ channel KCa3.1 and CD4 T-cells
    Shekhar Srivastava
    Division of Nephrology, Department of Pharmacology, The Helen L and Martin S Kimmel Center for Biology and Medicine at the Skirball Institute for Biomolecular Medicine, New York University Langone Medical Center, New York, NY 10016
    Mol Biol Cell 20:3783-91. 2009
    ..This is the first demonstration that a class II PI3K plays a critical role in T-cell activation...
  32. doi request reprint Visualization of cell-cell interaction contacts-synapses and kinapses
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine and Department of Pathology, New York University School of Medicine, 540 1st Ave, New York, NY 10016, USA
    Adv Exp Med Biol 640:164-82. 2008
    ..Optimal effector functions may also depend upon cyclical use of synapses and kinapses. Visualization of these structures in vitro and in vivo presents many distinct challenges that will be discussed in this chapter...
  33. doi request reprint T-cell activation through immunological synapses and kinapses
    Michael L Dustin
    Helen and Martin Kimmel Center for Biology and Medicine of the Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Immunol Rev 221:77-89. 2008
    ..Synapses and kinapses are inter-convertible by symmetrization/symmetry breaking processes, and both modes appear to be involved in normal T-cell priming. Imbalance of synapse/kinapse states may lead to immunopathology...
  34. pmc Tug of war at the exit door
    Michael L Dustin
    The Helen L and Martin S Kimmel Center for Biology and Medicine at the Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Immunity 28:15-7. 2008
    ..The lipid sphingosine-1-phosphate has been identified as a key exit signal for lymph nodes. In this issue of Immunity, Pham et al. (2008) show that its action can only be understood in the context of retention signals transduced by CCR7...
  35. pmc The immunological synapse
    Michael L Dustin
    Department of Pathology, New York University School of Medicine, Skirball Institute for Biomolecular Medicine, New York 10016, USA
    Arthritis Res 4:S119-25. 2002
    ..This chapter considers four aspects of the immunological synapse: the role of migration and stop signals, the role of the cytoskeleton, the role of self-antigenic complexes, and the role of second signals...
  36. ncbi request reprint Stop and go traffic to tune T cell responses
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10021, USA
    Immunity 21:305-14. 2004
    ..I present a model in which TCR stop signals compete with chemokine-mediated go signals to adjust the duration of immunological synapse formation and tune the immune response between tolerance and full activation...
  37. ncbi request reprint Membranes as messengers in T cell adhesion signaling
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine and the Department of Pathology, NYU School of Medicine, New York, New York 10016, USA
    Nat Immunol 5:363-72. 2004
    ..Speedy delivery of signals may be crucial, and membrane trafficking from endosomes and the Golgi apparatus seem to be essential in delivering the messages from spatially segregated surface receptors...
  38. pmc Membrane domains and the immunological synapse: keeping T cells resting and ready
    Michael L Dustin
    Department of Pathology and Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA
    J Clin Invest 109:155-60. 2002
  39. ncbi request reprint A dynamic view of the immunological synapse
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, and Department of Pathology, New York University School of Medicine, 540 1st Ave, New York, NY 10016, USA
    Semin Immunol 17:400-10. 2005
    ..IS and HS operate in different stages of T cell priming. Optimal effector functions may also depend upon cyclical use of IS and HS...
  40. ncbi request reprint Immunology. When F-actin becomes too much of a good thing
    Michael L Dustin
    Progam in Molecular Pathogenesis, Skirball Institute, New York University Medical Center, 540 First Avenue, New York, NY 10016, USA
    Science 313:767-8. 2006
  41. ncbi request reprint Coordination of T cell activation and migration through formation of the immunological synapse
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine and the Department of Pathology, New York University School of Medicine, New York, New York 10016, USA
    Ann N Y Acad Sci 987:51-9. 2003
    ..I propose that this molecular pattern and its specific biochemical constituents are necessary to amplify signals from the partially desensitized TCR...
  42. pmc Human immunodeficiency virus type 1 envelope gp120 induces a stop signal and virological synapse formation in noninfected CD4+ T cells
    Gaia Vasiliver-Shamis
    VA Medical Center, 423 E 23rd St, Room 18 124 North, New York, NY 10010, USA
    J Virol 82:9445-57. 2008
    ..The virological synapse was formed transiently, with the initiation of migration within 30 min. Thus, HIV-1 gp120-presenting surfaces induce a transient stop signal and supramolecular segregation in noninfected CD4(+) T cells...
  43. ncbi request reprint Neural and immunological synaptic relations
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, Department of Pathology, New York University School of Medicine, New York, NY 10016 USA
    Science 298:785-9. 2002
    ..Surface molecules that may be incorporated into and around the active zones contribute to modulation of the functional state of the synapse...
  44. pmc Cell adhesion molecules and actin cytoskeleton at immune synapses and kinapses
    Michael L Dustin
    Program in Molecular Pathogenesis, Helen L and Martin S Kimmel Center for Biology and Medicine of the Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, United States
    Curr Opin Cell Biol 19:529-33. 2007
    ..Breaking the symmetry of an immunological synapse generates a moving adhesive junction that can be defined as a kinapse, which facilitates signal integration by immune cells while moving over the surface of antigen-presenting cells...
  45. ncbi request reprint Quantification and modeling of tripartite CD2-, CD58FC chimera (alefacept)-, and CD16-mediated cell adhesion
    Michael L Dustin
    Department of Pathology, New York University School of Medicine and Helen L and Martin S Kimmel Center for Biology and Medicine of Skirball Institute of Biomolecular Medicine, New York, New York 10016, USA
    J Biol Chem 282:34748-57. 2007
    ..These results suggest that additional information is needed to correctly predict Alefacept-mediated bridge formation...
  46. ncbi request reprint Shmoos, rafts, and uropods- the many facets of cell polarity
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Cell 110:13-8. 2002
    ..Across these diverse biological and molecular systems, important general concepts emerged, including new ideas for establishing and maintaining polarity that are likely to be applicable across models and experimental systems...
  47. ncbi request reprint Impact of the immunological synapse on T cell signaling
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, and Department of Pathology, New York University School of Medicine, NY 10016, USA
    Results Probl Cell Differ 43:175-98. 2006
    ..IS and HS dominate in different stages of T cell priming. Optimal effector functions may also depend upon cyclical use of IS and HS...
  48. pmc Germinal center dynamics revealed by multiphoton microscopy with a photoactivatable fluorescent reporter
    Gabriel D Victora
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA
    Cell 143:592-605. 2010
    ..Thus, T cell help, and not direct competition for antigen, is the limiting factor in GC selection...
  49. pmc Monocyte trafficking to hepatic sites of bacterial infection is chemokine independent and directed by focal intercellular adhesion molecule-1 expression
    Chao Shi
    Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    J Immunol 184:6266-74. 2010
    ..monocytogenes-infected liver does not require chemokine receptor-mediated signals but instead is principally dependent on integrin- and extracellular matrix-mediated monocyte adhesion...
  50. pmc Cytotoxic T lymphocytes form an antigen-independent ring junction
    Kristina Somersalo
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, New York, New York 10016, USA
    J Clin Invest 113:49-57. 2004
    ..This result has specific implications for the mechanism of effective CTL hunting for antigen in tissues. Abnormalities in this process may alter CTL reactivity...
  51. pmc Affinity measured by microcluster
    David R Fooksman
    Martin and Helen Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    J Exp Med 207:907-9. 2010
    ..New work suggests that the early dynamics of BCR mobility and microcluster formation "translate" BCR affinity for antigen into B cell responsiveness...
  52. pmc Intravascular immune surveillance by CXCR6+ NKT cells patrolling liver sinusoids
    Frederic Geissmann
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, USA
    PLoS Biol 3:e113. 2005
    ..Thus, NKT cells patrol liver sinusoids to provide intravascular immune surveillance, and CXCR6 contributes to liver-based immune responses by regulating their abundance...
  53. pmc Human immunodeficiency virus type 1 envelope gp120-induced partial T-cell receptor signaling creates an F-actin-depleted zone in the virological synapse
    Gaia Vasiliver-Shamis
    Program in Molecular Pathogenesis, Marty and Helen Kimmel Center for Biology and Medicine, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Virol 83:11341-55. 2009
    ..We propose a model in which the F-actin-depleted zone formed within the target CD4 T cell enhances the reception of virions by releasing the physical barrier for HIV-1 entry and facilitating postentry events...
  54. pmc T cell receptor-proximal signals are sustained in peripheral microclusters and terminated in the central supramolecular activation cluster
    Rajat Varma
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, Department of Pathology, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA
    Immunity 25:117-27. 2006
    ..Our studies reveal a role for F-actin in TCR signaling beyond microcluster formation...
  55. ncbi request reprint Cutting edge: activation by innate cytokines or microbial antigens can cause arrest of natural killer T cell patrolling of liver sinusoids
    Peter Velazquez
    Molecular Pathogenesis Program, The Helen L and Martin S Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York, NY 10016, USA
    J Immunol 180:2024-8. 2008
    ..Interestingly, NKT cell arrest also results from IL-12 and IL-18 synergistic activation. Thus, innate cytokines and natural microbial TCR agonists trigger sinusoidal NKT cell arrest and an effector response...
  56. ncbi request reprint In vivo imaging of germinal centres reveals a dynamic open structure
    Tanja A Schwickert
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10021, USA
    Nature 446:83-7. 2007
    ..We conclude that the open structure of germinal centres enhances competition and ensures that rare high-affinity B cells can participate in antibody responses...
  57. ncbi request reprint T cell-dendritic cell immunological synapses
    Michael L Dustin
    Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine and Department of Pathology, New York University School of Medicine, NY 10016, USA
    Curr Opin Immunol 18:512-6. 2006
    ..Recent studies on model systems provide insight into the mechanisms and biological consequences of the unique T cell-DC synaptic patterns...
  58. ncbi request reprint Small GTPases and LFA-1 reciprocally modulate adhesion and signaling
    Adam Mor
    Department of Medicine, NYU School of Medicine, New York, NY 10016, USA
    Immunol Rev 218:114-25. 2007
    ..We have recently shown that Ras is also downstream of LFA-1 engagement: LFA-1 signaling through phospholipase D (PLD) to RasGRP1 was required for Ras activation on the plasma membrane following stimulation of TCR...
  59. ncbi request reprint Innate response to focal necrotic injury inside the blood-brain barrier
    Jiyun V Kim
    Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
    J Immunol 177:5269-77. 2006
    ..The astrocytes network established a cytoplasmic Ca2+ gradient that preceded the microglial response. This is consistent with astrocyte-microglial collaboration to mount this innate response that excludes blood leukocytes...
  60. ncbi request reprint Lateral membrane waves constitute a universal dynamic pattern of motile cells
    Hans Günther Döbereiner
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Phys Rev Lett 97:038102. 2006
    ..These wave patterns indicate both spatial and temporal long-range periodic correlations of the actomyosin gel...
  61. pmc Feedback control of regulatory T cell homeostasis by dendritic cells in vivo
    Guillaume Darrasse-Jèze
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA
    J Exp Med 206:1853-62. 2009
    ..The increase in T reg cells induced by DC expansion is sufficient to prevent type 1 autoimmune diabetes and IBD, which suggests that interference with this feedback loop will create new opportunities for immune-based therapies...
  62. doi request reprint Multiscale analysis of T cell activation: correlating in vitro and in vivo analysis of the immunological synapse
    Michael L Dustin
    Department of Pathology, Program of Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, NYU School of Medicine, 540 First Avenue, New York, NY 10016, USA
    Curr Top Microbiol Immunol 334:47-70. 2009
    ..In vivo decisions by T cells on stopping or migration are based on antigen stop signals and environmental go signals that can sometimes prevent arrest of T cells altogether, and thus can change the outcome of antigen encounters...
  63. ncbi request reprint ATP mediates rapid microglial response to local brain injury in vivo
    Dimitrios Davalos
    Molecular Neurobiology Program, Department of Physiology and Neuroscience, New York University School of Medicine, 540 First Avenue, New York, New York 10016, USA
    Nat Neurosci 8:752-8. 2005
    ..Thus, extracellular ATP regulates microglial branch dynamics in the intact brain, and its release from the damaged tissue and surrounding astrocytes mediates a rapid microglial response towards injury...
  64. pmc CCR7 signalling as an essential regulator of CNS infiltration in T-cell leukaemia
    Silvia Buonamici
    Department of Pathology and New York University Cancer Institute, New York 10016, USA
    Nature 459:1000-4. 2009
    ..Targeted inhibition of CNS involvement in T-ALL could potentially decrease the intensity of CNS-targeted therapy, thus reducing its associated short- and long-term complications...

Research Grants22

  1. REQUIREMENT FOR SENSITIVE T CELL RESPONSE TO ANTIGEN
    Michael Dustin; Fiscal Year: 2005
    ....
  2. PHYSIOLOGICAL CHEMISTRY OF INTEGRIN FUNCTION
    Michael Dustin; Fiscal Year: 2007
    ..These experiments should provide new insight into regulation of integrin function and identify potential therapeutic targets for regulation of leukocyte integrins. ..
  3. Environmental Control of the Immunological Synapse
    Michael Dustin; Fiscal Year: 2007
    ..In Aim 3 we will investigate the basis of dominant and subordinate chemokine behavior and will seek to modify the hierarchy in vivo to test the impact of these hierarchies on the primary immune response. ..
  4. PHYSIOLOGICAL CHEMISTRY OF INTEGRIN FUNCTION
    Michael Dustin; Fiscal Year: 2002
    ..In Aim 3 we will determine the most effective strategy to probe integrin cytoskeletal interactions through expression of exogenous cytoplasmic domain. ..
  5. Environmental Control of the Immunological Synapse
    MICHAEL LORAN DUSTIN; Fiscal Year: 2010
    ..We propose experiments to determine the roles of chemokines, dendritic cell frequency and regulatory T cell protein kinase C-8 in tolerance induction. ..