Z Darzynkiewicz

Summary

Affiliation: New York Medical College
Country: USA

Publications

  1. pmc Genome integrity, stem cells and hyaluronan
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Aging (Albany NY) 4:78-88. 2012
  2. pmc DNA damage signaling assessed in individual cells in relation to the cell cycle phase and induction of apoptosis
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Crit Rev Clin Lab Sci 49:199-217. 2012
  3. pmc ATM activation accompanies histone H2AX phosphorylation in A549 cells upon exposure to tobacco smoke
    Toshiki Tanaka
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    BMC Cell Biol 8:26. 2007
  4. ncbi request reprint Chinese herbal mixture PC SPES in treatment of prostate cancer (review)
    Z Darzynkiewicz
    Brander Cancer Research Institute at NYMC, Hawthorne, NY 10532, USA
    Int J Oncol 17:729-36. 2000
  5. ncbi request reprint One more reason to get arrested (in G1)
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, 19 Bradhurst Ave, Hawthorne, New York 10532, USA
    Cell Cycle 1:318-9. 2002
  6. pmc Cytometry of DNA replication and RNA synthesis: Historical perspective and recent advances based on "click chemistry"
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 79:328-37. 2011
  7. pmc Laser scanning cytometry for automation of the micronucleus assay
    Zbigniew Darzynkiewicz
    Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Mutagenesis 26:153-61. 2011
  8. ncbi request reprint Use of flow and laser scanning cytometry to study mechanisms regulating cell cycle and controlling cell death
    Z Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Clin Lab Med 21:857-73. 2001
  9. pmc Analysis of cellular DNA content by flow and laser scanning cytometry
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute at NYMC, Department of Pathology, BSB 438, Valhalla, New York 10595, USA
    Adv Exp Med Biol 676:137-47. 2010
  10. ncbi request reprint All that glitters is not gold: all that FLICA binds is not caspase. A caution in data interpretation--and new opportunities
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 71:536-7. 2007

Research Grants

  1. PROBES FOR CYTOMETRY
    Zbigniew Darzynkiewicz; Fiscal Year: 2009
  2. PROBES FOR FLOW CYTOMETRY
    Zbigniew Darzynkiewicz; Fiscal Year: 1999
  3. PROBES FOR FLOW CYTOMETRY
    Zbigniew Darzynkiewicz; Fiscal Year: 1993
  4. PROBES FOR CYTOMETRY
    Zbigniew Darzynkiewicz; Fiscal Year: 2010

Collaborators

Detail Information

Publications128 found, 100 shown here

  1. pmc Genome integrity, stem cells and hyaluronan
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Aging (Albany NY) 4:78-88. 2012
    ..While HA has multitude of functions its anti-oxidant capabilities are often overlooked but may be of significance in preservation of integrity of stem and germ cells genome...
  2. pmc DNA damage signaling assessed in individual cells in relation to the cell cycle phase and induction of apoptosis
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Crit Rev Clin Lab Sci 49:199-217. 2012
    ..Critically assessed is interpretation of the data as to whether the observed DDS events report induction of a particular type of DNA lesion...
  3. pmc ATM activation accompanies histone H2AX phosphorylation in A549 cells upon exposure to tobacco smoke
    Toshiki Tanaka
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    BMC Cell Biol 8:26. 2007
    ..We have recently reported that brief exposure of human pulmonary adenocarcinoma A549 cells or normal human bronchial epithelial cells (NHBE) to cigarette smoke (CS) induced phosphorylation of H2AX...
  4. ncbi request reprint Chinese herbal mixture PC SPES in treatment of prostate cancer (review)
    Z Darzynkiewicz
    Brander Cancer Research Institute at NYMC, Hawthorne, NY 10532, USA
    Int J Oncol 17:729-36. 2000
    ....
  5. ncbi request reprint One more reason to get arrested (in G1)
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, 19 Bradhurst Ave, Hawthorne, New York 10532, USA
    Cell Cycle 1:318-9. 2002
  6. pmc Cytometry of DNA replication and RNA synthesis: Historical perspective and recent advances based on "click chemistry"
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 79:328-37. 2011
    ..Several examples are presented that illustrate incorporation of EdU or EU in cells subjected to DNA damage detected as histone H2AX phosphorylation that have been analyzed by flow or laser scanning cytometry...
  7. pmc Laser scanning cytometry for automation of the micronucleus assay
    Zbigniew Darzynkiewicz
    Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Mutagenesis 26:153-61. 2011
    ..e. exfoliated cells, dermal cell models, etc.) hold great promise for robust and exciting developments in MN assay automation as a high-content high-throughput analysis procedure...
  8. ncbi request reprint Use of flow and laser scanning cytometry to study mechanisms regulating cell cycle and controlling cell death
    Z Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Clin Lab Med 21:857-73. 2001
    ..One of the dominating motors for this progress will be further development of cytometry instrumentation and the applicability of this instrumentation, methods, and probes...
  9. pmc Analysis of cellular DNA content by flow and laser scanning cytometry
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute at NYMC, Department of Pathology, BSB 438, Valhalla, New York 10595, USA
    Adv Exp Med Biol 676:137-47. 2010
    ..Described also are controls used to ensure accuracy of DNA ploidy determination, the principles in ploidy assessment and possible pitfalls in analysis...
  10. ncbi request reprint All that glitters is not gold: all that FLICA binds is not caspase. A caution in data interpretation--and new opportunities
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 71:536-7. 2007
  11. pmc Impaired DNA damage response--an Achilles' heel sensitizing cancer to chemotherapy and radiotherapy
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY, USA
    Eur J Pharmacol 625:143-50. 2009
    ..Antitumor strategies are being designed to treat cancers having particular defects in their DDR, concurrent with protecting normal cells...
  12. pmc Fluoroquinolones lower constitutive H2AX and ATM phosphorylation in TK6 lymphoblastoid cells via modulation of the intracellular redox status
    H Dorota Halicka
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10595, USA
    Pharmacol Rep 61:711-8. 2009
    ..We presume that FQs mediate this effect via a combination of ROS-scavenging and mitochondrial suppression and therefore may protect against the onset or may slow the progression of numerous oxidative pathophysiological conditions...
  13. ncbi request reprint Cytometry of the cell cycle: cycling through history
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10532, USA
    Cytometry A 58:21-32. 2004
  14. pmc There's more than one way to skin a cat: yet another way to assess mitotic index by cytometry
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 73:386-7. 2008
  15. pmc Analysis of apoptosis by cytometry using TUNEL assay
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Basic Sciences Building, Valhalla, NY 10595, USA
    Methods 44:250-4. 2008
    ..Concurrent staining of DNA with propidium or 4',6-diamidino-2-phenylindole (DAPI) and multiparameter analysis of cells by flow- or laser scanning cytometry enables one to correlate induction of apoptosis with the cell cycle phase...
  16. pmc Cell synchronization by inhibitors of DNA replication induces replication stress and DNA damage response: analysis by flow cytometry
    Zbigniew Darzynkiewicz
    Department of Pathology, Brander Cancer Research Institute, New York Medical College, 10595, Valhalla, NY, USA
    Methods Mol Biol 761:85-96. 2011
    ..Concurrent measurement of cellular DNA content and phosphorylated proteins followed by multiparameter cytometric analysis allows one to correlate extent of their phosphorylation with cell cycle phase...
  17. ncbi request reprint Cytometry of cyclin proteins
    Z Darzynkiewicz
    Cancer Research Institute, New York Medical College, Valhalla, USA
    Cytometry 25:1-13. 1996
    ..As sensitive and specific indicators of the cell's proliferative potential, the cyclins, in particular D-type cyclins, are expected to be key prognostic markers in neoplasia...
  18. ncbi request reprint Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis)
    Z Darzynkiewicz
    The Cancer Research Institute, New York Medical College, Valhalla, New York 10523, USA
    Cytometry 27:1-20. 1997
    ..It is expected that flow cytometry will be the dominant methodology for necrobiology...
  19. ncbi request reprint Critical aspects in the analysis of apoptosis and necrosis
    Z Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10595, USA
    Hum Cell 11:3-12. 1998
    ..By virtue of this attribute LSC appears to be the instrument of choice for analysis of apoptosis...
  20. ncbi request reprint Laser-scanning cytometry: A new instrumentation with many applications
    Z Darzynkiewicz
    The Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10595, USA
    Exp Cell Res 249:1-12. 1999
    ..g., for archival preservation or additional analysis. Potential future applications of LSC are discussed...
  21. ncbi request reprint Measurement of apoptosis
    Z Darzynkiewicz
    Cancer Research Institute, New York Medical College, Elmsford 10523, USA
    Adv Biochem Eng Biotechnol 62:33-73. 1998
    ..The most common pitfalls and improper use of the methodology in analysis of cell death and in data interpretation are also discussed...
  22. ncbi request reprint Laser scanning cytometric analysis of cyclin B1 in primary human malignancies
    W Gorczyca
    Department of Pathology, New York Medical College, Valhalla 10523, USA
    Mod Pathol 10:457-62. 1997
    ..The data suggest that the "unscheduled" expression of cyclin B1 might be a common defect in neoplasia...
  23. ncbi request reprint Selective protection of mitogenically stimulated human lymphocytes but not leukemic cells from cytosine arabinoside-induced apoptosis by LY294002, a phosphoinositol-3 kinase inhibitor
    L Du
    Brander Cancer Research Institute, New York Medical College, Hawthorne, NY 10532, USA
    Int J Oncol 19:811-9. 2001
    ..Because this LY-induced suppression was stronger in Jurkat cells (>70%) than in lymphocytes (20%), the proapoptotic effects of PKB/Akt down-regulation appear to be selective towards tumor cells...
  24. ncbi request reprint Extent of constitutive histone H2AX phosphorylation on Ser-139 varies in cells with different TP53 status
    T Tanaka
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10595, USA
    Cell Prolif 39:313-23. 2006
    ....
  25. ncbi request reprint Detection of caspases activation by fluorochrome-labeled inhibitors: Multiparameter analysis by laser scanning cytometry
    P Smolewski
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10532, USA
    Cytometry 44:73-82. 2001
    ..FLICA can also be used to study intracellular localization of caspases, including their translocation...
  26. ncbi request reprint Morphometry of nucleoli and expression of nucleolin analyzed by laser scanning cytometry in mitogenically stimulated lymphocytes
    W Gorczyca
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cytometry 45:206-13. 2001
    ..The capability of LSC to measure the protein translocation between nucleolus and nucleoplasm can be used to study the function and regulatory mechanisms of other proteins that reside in these compartments...
  27. pmc Telomere shortening is an in vivo marker of myocyte replication and aging
    J Kajstura
    Department of Medicine, New York Medical College, Valhalla, New York 10595, USA
    Am J Pathol 156:813-9. 2000
    ..In conclusion, a significant fraction of myocytes divides repeatedly from birth to senescence, counteracting the continuous death of cells in the aging mammalian rat heart...
  28. ncbi request reprint Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis
    G Juan
    The Cancer Research Institute, New York Medical College, Valhalla, USA
    Cytometry 32:71-7. 1998
    ..It also offers an assay to screen in vivo inhibitors of kinase(s) or phosphatase(s) involved in H3 phosphorylation or dephosphorylation, and it provides a valuable marker to identify mitotic cells by cytometry...
  29. ncbi request reprint Constitutive histone H2AX phosphorylation and ATM activation are strongly amplified during mitogenic stimulation of lymphocytes
    T Tanaka
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Cell Prolif 40:1-13. 2007
    ..The aim of this study was to seek further evidence in support of this postulate, namely to test whether the levels of CAA and CHP correlate with cells metabolic activity...
  30. ncbi request reprint Histone H3 phosphorylation in human monocytes and during HL-60 cell differentiation
    G Juan
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, 10595, USA
    Exp Cell Res 246:212-20. 1999
    ..The high level of H3 phosphorylation in monocytes may serve as a marker of these cells and is being explored as a possible diagnostic and prognostic tool in monocytic leukemias...
  31. ncbi request reprint Effects of the flavonoid baicalin and its metabolite baicalein on androgen receptor expression, cell cycle progression and apoptosis of prostate cancer cell lines
    S Chen
    Brander Cancer Research Institute, New York Medical College, Hawthorne, NY 10532, USA
    Cell Prolif 34:293-304. 2001
    ..Both agents (1) caused an apparent accumulation of cells in G(1) at the ED(50) concentration, (2) induced apoptosis at higher concentrations, and (3) decreased expression of the androgen receptor in LNCaP cells...
  32. ncbi request reprint Bivariate analysis of cellular DNA versus RNA content by laser scanning cytometry using the product of signal subtraction (differential fluorescence) as a separate parameter
    P Smolewski
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10523, USA
    Cytometry 45:73-8. 2001
    ..The method can be extended for multiparameter analysis of cell populations stained with other fluorochromes of the same-wavelength emission but targeted (e.g., immunocytochemically) for different cell constituents...
  33. ncbi request reprint 2-deoxy-D-glucose reduces the level of constitutive activation of ATM and phosphorylation of histone H2AX
    Toshiki Tanaka
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10532, USA
    Cell Cycle 5:878-82. 2006
    ..Therefore, the assay of CHP by multiparameter cytometry provides the means to measure effects of antioxidants and metabolic inhibitors on endogenous oxidative DNA damage in relation to cell cycle phase...
  34. ncbi request reprint Cell cycle arrest and apoptosis of melanoma cells by docosahexaenoic acid: association with decreased pRb phosphorylation
    A P Albino
    The American Health Foundation, Valhalla, New York 10595, USA
    Cancer Res 60:4139-45. 2000
    ..Determining the mechanism by which PUFAs can inhibit melanoma growth will be an important first step in the rational use of PUFAs as antitumor agents...
  35. ncbi request reprint Analysis of apoptosis in solid tumors by laser-scanning cytometry
    W Gorczyca
    Department of Pathology, New York Medical College, Valhalla 10595, USA
    Mod Pathol 11:1052-8. 1998
    ..Our results suggest that different morphologic variants of apoptosis might be common in different tumor types and that cytometric quantification might provide biologically useful information not otherwise easy to obtain...
  36. ncbi request reprint Enhancement of activation-induced apoptosis of lymphocytes by the cytotoxic ribonuclease onconase (Ranpirnase)
    D H Halicka
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY, USA
    Int J Oncol 21:1245-50. 2002
    ..The propensity of Onc to potentiate the activation-induced apoptosis suggests that this drug may have clinical utility as immunomodulating agent, e.g., to suppress transplant rejection or treat autoimmune diseases...
  37. ncbi request reprint Activation of ATM and histone H2AX phosphorylation induced by mitoxantrone but not by topotecan is prevented by the antioxidant N-acetyl-L-cysteine
    Xuan Huang
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10595, USA
    Cancer Biol Ther 5:959-64. 2006
    ....
  38. ncbi request reprint Sequential phosphorylation of Ser-10 on histone H3 and ser-139 on histone H2AX and ATM activation during premature chromosome condensation: relationship to cell-cycle phase and apoptosis
    Xuan Huang
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10532, USA
    Cytometry A 69:222-9. 2006
    ....
  39. ncbi request reprint Induction of ATM activation, histone H2AX phosphorylation and apoptosis by etoposide: relation to cell cycle phase
    Toshiki Tanaka
    Brander Cancer Research Institute, Department of Pathology, New York Medical College, Valhalla, New York 10595, USA
    Cell Cycle 6:371-6. 2007
    ..mitoxantrone, may contribute to formation of DSBs and to triggering of apoptosis...
  40. ncbi request reprint Synchronization in the cell cycle by inhibitors of DNA replication induces histone H2AX phosphorylation: an indication of DNA damage
    A Kurose
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10595, USA
    Cell Prolif 39:231-40. 2006
    ..The above effects should be taken into account when interpreting data obtained with the use of cells synchronized by inhibitors of DNA replication...
  41. ncbi request reprint "Liquidless" cell staining by dye diffusion from gels and analysis by laser scanning cytometry: potential application at microgravity conditions in space
    P Smolewski
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10532, USA
    Cytometry 44:355-60. 2001
    ..The quality of the staining provided by this methodology is comparable to conventional cell staining in dye solutions...
  42. ncbi request reprint Laser scanning cytometer (LSC) analysis of fraction of labelled mitoses (FLM)
    W Gorczyca
    Department of Pathology, New York Medical College, Valhalla 10595, USA
    Cell Prolif 29:539-47. 1996
    ..The ability to automatically and rapidly discriminate mitotic cells combined with the possibility of their subsequent identification by image analysis makes LSC the instrument of choice for the FLM analysis...
  43. ncbi request reprint ATM activation and histone H2AX phosphorylation as indicators of DNA damage by DNA topoisomerase I inhibitor topotecan and during apoptosis
    T Tanaka
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10532, USA
    Cell Prolif 39:49-60. 2006
    ..The data suggest that frequency of activated ATM and phosphorylated H2AX molecules, per apoptotic cell, is comparable...
  44. ncbi request reprint Segregation of RNA and separate packaging of DNA and RNA in apoptotic bodies during apoptosis
    H D Halicka
    Brander Cancer Research Institute, Hawthorne, New York, 10532, USA
    Exp Cell Res 260:248-56. 2000
    ..Such specialization may facilitate heterophagic degradation of nucleic acids during apoptosis...
  45. pmc Kinetics of the UV-induced DNA damage response in relation to cell cycle phase. Correlation with DNA replication
    Hong Zhao
    Department of Pathology, Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 77:285-93. 2010
    ....
  46. ncbi request reprint Polo box domain of Plk3 functions as a centrosome localization signal, overexpression of which causes mitotic arrest, cytokinesis defects, and apoptosis
    Ning Jiang
    Division of Molecular Carcinogenesis, Department of Medicine, New York Medical College, Basic Science Building, Valhalla, NY 10595, USA
    J Biol Chem 281:10577-82. 2006
    ....
  47. ncbi request reprint Effects of hydroxyurea and aphidicolin on phosphorylation of ataxia telangiectasia mutated on Ser 1981 and histone H2AX on Ser 139 in relation to cell cycle phase and induction of apoptosis
    Akira Kurose
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cytometry A 69:212-21. 2006
    ..The present study was undertaken to reveal whether H2AX is phosphorylated in cells exposed to HU or APH and whether its phosphorylation is mediated by ATM...
  48. pmc Phosphorylation of p53 on Ser15 during cell cycle caused by Topo I and Topo II inhibitors in relation to ATM and Chk2 activation
    Hong Zhao
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, New York 10595, USA
    Cell Cycle 7:3048-55. 2008
    ..The respective kinetics of p53-Ser15 phosphorylation versus ATM and Chk2 activation suggest that in response to DNA damage by TPT or MXT, Chk2 rather than ATM mediates p53 phosphorylation...
  49. pmc Oxidative stress induces cell cycle-dependent Mre11 recruitment, ATM and Chk2 activation and histone H2AX phosphorylation
    Hong Zhao
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, New York 10595, USA
    Cell Cycle 7:1490-5. 2008
    ..g., maximal pixel) offers unique advantage in studies of the response of different cell constituents in relation to cell cycle position...
  50. ncbi request reprint Interactions of fluorochrome-labeled caspase inhibitors with apoptotic cells: a caution in data interpretation
    P Pozarowski
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cytometry A 55:50-60. 2003
    ..We have recently observed, however, that their binding is more complex and may involve additional mechanisms. Our goal in this study was to clarify the ongoing utility of these probes...
  51. ncbi request reprint Immunoseparation and immunodetection of nucleic acids labeled with halogenated nucleotides
    S R Haider
    The Cancer Research Institute, New York Medical College, Valhalla 10595, USA
    Exp Cell Res 234:498-506. 1997
    ..Due to the low toxicity of BrU and BrdU, this approach can be used in analysis of gene transcription or DNA replication in vivo...
  52. ncbi request reprint Stathmo-apoptosis: arresting apoptosis by fluorochrome-labeled inhibitor of caspases
    P Smolewski
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10595, USA
    Int J Oncol 19:657-63. 2001
    ..The present approach offers a unique capability to accurately estimate the kinetics of cell transition to apoptosis, revealing the unbiased cumulative apoptotic index over a long time span after induction of apoptosis...
  53. ncbi request reprint Cytometric assessment of DNA damage by exogenous and endogenous oxidants reports aging-related processes
    Hong Zhao
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 71:905-14. 2007
    ..Cytometric analysis of CHP and CAA provides the means to measure the effectiveness of exogenous factors, which either through lowering aerobic metabolism or neutralizing radicals may protect DNA from such damage...
  54. ncbi request reprint Cytometry of ATM activation and histone H2AX phosphorylation to estimate extent of DNA damage induced by exogenous agents
    Toshiki Tanaka
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 71:648-61. 2007
    ....
  55. ncbi request reprint Assessment of ATM phosphorylation on Ser-1981 induced by DNA topoisomerase I and II inhibitors in relation to Ser-139-histone H2AX phosphorylation, cell cycle phase, and apoptosis
    Akira Kurose
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10532, USA
    Cytometry A 68:1-9. 2005
    ....
  56. pmc Biscoclaurine alkaloid cepharanthine protects DNA in TK6 lymphoblastoid cells from constitutive oxidative damage
    Dorota Halicka
    Branch Cancer Reearch Institute and Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Pharmacol Rep 60:93-100. 2008
    ..3 microM CEP for 24 h. The data strongly suggest that the scavenging properties of CEP provide a protection of DNA from the radicals generated endogenously during oxidative metabolism...
  57. pmc Protective effect of hyaluronate on oxidative DNA damage in WI-38 and A549 cells
    Hong Zhao
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Int J Oncol 32:1159-67. 2008
    ..Since no significant degradation of HA upon its exposure in tissue culture medium to H2O2 was detected the scavenging may occur intracellularly...
  58. pmc Induction of DNA damage response by the supravital probes of nucleic acids
    Hong Zhao
    Department of Pathology, Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 75:510-9. 2009
    ..The data indicate that supravital use of Ho 42, DRAQ5, and DCV induces various degrees of DDR, including activation of ATM, Chk2 and p53, which may have significant consequences on regulatory cell cycle pathways and apoptosis...
  59. ncbi request reprint RIalpha influences cellular proliferation in cancer cells by transporting RFC40 into the nucleus
    Rakhee S Gupte
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    Cancer Biol Ther 4:429-37. 2005
    ..These findings provide evidence for a previously unknown mechanism for the nuclear transport of RFC40 and also for a novel mechanism for cellular proliferation...
  60. ncbi request reprint DNA content measurement for DNA ploidy and cell cycle analysis
    Z Darzynkiewicz
    New York Medical College, Valhalla, New York, USA
    Curr Protoc Cytom . 2001
    ..Protocols are given for analysis of fixed cells, tissue samples, live cells (for subsequent sorting and culture), archival paraffin-embedded tissue, and apoptotic cells...
  61. ncbi request reprint Bivariate analysis of DNA content and expression of cyclin proteins
    G Juan
    New York Medical College, Valhalla, New York, USA
    Curr Protoc Cytom . 2001
    ....
  62. ncbi request reprint Analysis of DNA content and BrdU incorporation
    Z Darzynkiewicz
    New York Medical College, Valhalla, New York, USA
    Curr Protoc Cytom . 2001
    ....
  63. ncbi request reprint Determining cell cycle stages by flow cytometry
    Z Darzynkiewicz
    New York Medical College, Valhalla, New York, USA
    Curr Protoc Cell Biol . 2001
    ..When DNA content measurements are coupled with immunostaining for cyclin expression, it is possible to reliably detect even more stages in progression through the cell cycle...
  64. ncbi request reprint Chlorophyllin protects cells from the cytostatic and cytotoxic effects of quinacrine mustard but not of nitrogen mustard
    B Ardelt
    Brander Cancer Research Institute at New York Medical College, 19 Bradhurst Avenue, Suite 2400, Hawthorne, NY 10532, USA
    Int J Oncol 18:849-53. 2001
    ..Therefore, excess of chlorophyll in the diet, by sequestering aromatic mutagens (or antitumor drugs with a heterocyclic structure, if taken orally), may inhibit their accessibility to cells, thereby reducing their activity...
  65. ncbi request reprint Constitutive histone H2AX phosphorylation on Ser-139 in cells untreated by genotoxic agents is cell-cycle phase specific and attenuated by scavenging reactive oxygen species
    Xuan Huang
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10595, USA
    Int J Oncol 29:495-501. 2006
    ....
  66. ncbi request reprint Nitrogen oxide-releasing aspirin induces histone H2AX phosphorylation, ATM activation and apoptosis preferentially in S-phase cells: involvement of reactive oxygen species
    Toshiki Tanaka
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, New York, USA
    Cell Cycle 5:1669-74. 2006
    ..Because neither induction of H2AX phosphorylation nor apoptosis were observed at equimolar concentrations of ASA, the NO moiety attached to ASA appeared to mediate these effects...
  67. ncbi request reprint Phosphorylation of histone H2AX on Ser 139 and activation of ATM during oxidative burst in phorbol ester-treated human leukocytes
    Toshiki Tanaka
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, New York 10595, USA
    Cell Cycle 5:2671-5. 2006
    ..The observed H2AX phosphorylation in lymphocytes may reflect their DNA damage by the superoxide ions propagating from the neighboring granulocytes and/or monocytes...
  68. ncbi request reprint DNA damage response as a biomarker in treatment of leukemias
    H Dorota Halicka
    Brander Cancer Research Institute, Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Cell Cycle 8:1720-4. 2009
    ..The present findings, however, demonstrate the feasibility of assessment of DDR during the treatment of leukemias with drugs targeting DNA...
  69. pmc Cytometric assessment of DNA damage in relation to cell cycle phase and apoptosis
    Xuan Huang
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10532, USA
    Cell Prolif 38:223-43. 2005
    ..Detection of DNA damage in tumour cells during radio- or chemotherapy may provide an early marker predictive of response to treatment...
  70. pmc Down-regulation of androgen-receptor and PSA by phytochemicals
    Sophie Chen
    Department of Medicine, New York Medical College, Valhalla, NY 10595, USA
    Int J Oncol 32:405-11. 2008
    ..In each case, these compounds have independent activities which may partly contribute to the biological activity of PC-SPES...
  71. pmc DNA damage response induced by tobacco smoke in normal human bronchial epithelial and A549 pulmonary adenocarcinoma cells assessed by laser scanning cytometry
    Hong Zhao
    New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 75:840-7. 2009
    ..The cytometric assessment of CS-induced DDR provides a means to estimate the genotoxicity of CS and to explore the mechanisms of the response as a function of cell cycle phase and cell type...
  72. ncbi request reprint Differential staining of DNA and RNA in intact cells and isolated cell nuclei with acridine orange
    Z Darzynkiewicz
    Cancer Research Institute, New York Medical College, Valhalla 10595
    Methods Cell Biol 33:285-98. 1990
  73. ncbi request reprint Differential expression of human topoisomerase IIIalpha during the cell cycle progression in HL-60 leukemia cells and human peripheral blood lymphocytes
    C W Lin
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    Exp Cell Res 256:225-36. 2000
    ..Its cellular level appeared to be under control during the cell cycle progression of normal lymphocytes, but was found to be deregulated in HL-60 cells which may be associated with the tumor transformed cell phenotypes...
  74. pmc Constitutive histone H2AX phosphorylation and ATM activation, the reporters of DNA damage by endogenous oxidants
    Toshiki Tanaka
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, New York 10595, USA
    Cell Cycle 5:1940-5. 2006
    ....
  75. ncbi request reprint Paclitaxel induces primary and postmitotic G1 arrest in human arterial smooth muscle cells
    Mikhail V Blagosklonny
    Brander Cancer Research Institute, New York Medical College, Hawthorne, New York, USA
    Cell Cycle 3:1050-6. 2004
    ..These post-mitotic cells were subsequently arrested in G(1) but maintained normal elongated morphology and were viable for at least 21 days. We conclude that in SMC PTX causes post-mitotic cell cycle arrest rather than cell death...
  76. ncbi request reprint Histone H2AX phosphorylation after cell irradiation with UV-B: relationship to cell cycle phase and induction of apoptosis
    H Dorota Halicka
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cell Cycle 4:339-45. 2005
    ..Induction of gammaH2AX in G(1), G(2) and M is likely a response to the primary DSBs generated during UV exposure and/or DNA repair. It is unclear why the latter process was more pronounced in HeLa than in HL-60 cells...
  77. ncbi request reprint Effects of resveratrol on the G(0)-G(1) transition and cell cycle progression of mitogenically stimulated human lymphocytes
    Tze Hsieh
    Brander Cancer Research Institute, New York Medical College, Hawthorne, NY 10532, USA
    Biochem Biophys Res Commun 297:1311-7. 2002
    ....
  78. ncbi request reprint Assessment of histone H2AX phosphorylation induced by DNA topoisomerase I and II inhibitors topotecan and mitoxantrone and by the DNA cross-linking agent cisplatin
    Xuan Huang
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10532, USA
    Cytometry A 58:99-110. 2004
    ..The purpose of this study was to further characterize the drug-induced (DI) IF of gammaH2AX, and in particular to distinguish it from AA gammaH2AX IF triggered by DNA breaks that occur in the course of AA DNA fragmentation...
  79. pmc Ciprofloxacin-induced G2 arrest and apoptosis in TK6 lymphoblastoid cells is not dependent on DNA double-strand break formation
    Daniel J Smart
    Department of Pathology, New York Medical College, Valhalla, New York 10595, USA
    Cancer Biol Ther 7:113-9. 2008
    ..The subsequent induction of apoptosis appears to be facilitated by functional p53. CPFX may therefore have a potential use as a chemotherapeutic agent in the treatment of lymphoblast-derived cancer...
  80. ncbi request reprint Arsenic trioxide arrests cells early in mitosis leading to apoptosis
    H Dorota Halicka
    Brander Cancer Research Institute, New York Medical College, Hawthorne, New York 10532, USA
    Cell Cycle 1:201-9. 2002
    ..Our data suggest that the antileukemic activities of arsenic may be a result of mitotic arrest which culminates in apoptosis...
  81. ncbi request reprint DNA damage induced by DNA topoisomerase I- and topoisomerase II-inhibitors detected by histone H2AX phosphorylation in relation to the cell cycle phase and apoptosis
    Xuan Huang
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cell Cycle 2:614-9. 2003
    ..The data also indicate that regardless whether treated with inhibitors of topo1 or topo2, at comparable levels of dsDNA breaks, the cells replicating DNA have a higher proclivity to undergo apoptosis compared to G1 or G2/M cells...
  82. doi request reprint Kinetics of histone H2AX phosphorylation and Chk2 activation in A549 cells treated with topotecan and mitoxantrone in relation to the cell cycle phase
    Hong Zhao
    Brander Cancer Research Institute, Department of Pathology, New York Medical College, Valhalla, New York 10595, USA
    Cytometry A 73:480-9. 2008
    ....
  83. ncbi request reprint Cytometric detection of chromatin relaxation, an early reporter of DNA damage response
    H Dorota Halicka
    Brander Cancer Research Institute and Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
    Cell Cycle 8:2233-7. 2009
    ..The method offers a rapid and simple means of detecting genotoxic insult on cells...
  84. ncbi request reprint BubR1 is involved in regulation of DNA damage responses
    Y Fang
    Division of Molecular Carcinogenesis, New York Medical College, Valhalla, NY 10595, USA
    Oncogene 25:3598-605. 2006
    ....
  85. ncbi request reprint Micronuclei assay by laser scanning cytometry
    P Smolewski
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cytometry 45:19-26. 2001
    ..These analytical capabilities of LSC may be helpful not only to score MN but also to study mechanisms by which clastogenic agents induce MN...
  86. ncbi request reprint Overview of nucleic acid analysis
    Z Darzynkiewicz
    New York Medical College, Valhalla, New York, USA
    Curr Protoc Cytom . 2001
    ....
  87. ncbi request reprint Induction of H2AX phosphorylation in pulmonary cells by tobacco smoke: a new assay for carcinogens
    A P Albino
    Vector Research Ltd, New York, New York 10019, USA
    Cell Cycle 3:1062-8. 2004
    ..The present assay to detect and measure DSBs induced by tobacco products complements other mutagenicity assays and may be applied to test potential carcinogens in other products...
  88. ncbi request reprint Analysis of DNA content and DNA strand breaks for detection of apoptotic cells
    Z Darzynkiewicz
    New York Medical College, Valhalla, New York, USA
    Curr Protoc Cytom . 2001
    ..Given the multiplicity of commercially available reagents it is possible to label DNA strand breaks with a dye of any fluorescence color and excitation wavelength...
  89. ncbi request reprint Licochalcone-A, a novel flavonoid isolated from licorice root (Glycyrrhiza glabra), causes G2 and late-G1 arrests in androgen-independent PC-3 prostate cancer cells
    Yue Fu
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Biochem Biophys Res Commun 322:263-70. 2004
    ..These findings provide mechanistic explanation for LA activity and suggest that it may be considered as a chemopreventive agent and its anticancer properties should be further explored...
  90. ncbi request reprint In vitro model of "wound healing" analyzed by laser scanning cytometry: accelerated healing of epithelial cell monolayers in the presence of hyaluronate
    Asifa S Haider
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY, USA
    Cytometry A 53:1-8. 2003
    ..The progress of healing is generally estimated by microscopy or time-lapse cinematography by assessing cell proliferation and/or migration that leads to the wound closure...
  91. ncbi request reprint Cytometry of cell cycle regulatory proteins
    Zbigniew Darzynkiewicz
    Cancer Research Institute, New York Medical College, Valhalla, N Y 10593, USA
    Prog Cell Cycle Res 5:533-42. 2003
    ..Particular attention is given to the use of multiparameter cytometry in analysis of the mechanism by which antitumor drugs affect cell cycle progression and induce cell cycle phase-specific apoptosis...
  92. pmc Cytometric assessment of histone H2AX phosphorylation: a reporter of DNA damage
    Xuan Huang
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY, USA
    Methods Mol Biol 314:73-80. 2006
    ..Intensity of cellular green (FITC) and red (PI) fluorescence is measured by flow cytometry and bivariate analysis of the data is used to correlate the presence of DSBs with the cell cycle phase...
  93. ncbi request reprint Analysis of DNA denaturation
    Z Darzynkiewicz
    New York Medical College, Elmsford, New York, USA
    Curr Protoc Cytom . 2001
    ..This unit provides full details on the correct use of acridine orange together with an excellent discussion of the pitfalls and problems...
  94. ncbi request reprint Flow cytometry in analysis of cell cycle and apoptosis
    Z Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY, USA
    Semin Hematol 38:179-93. 2001
    ..Semin Hematol 38:179-193...
  95. ncbi request reprint Combined effects of onconase and IFN-beta on proliferation, macromolecular syntheses and expression of STAT-1 in JCA-1 cancer cells
    S Y Tsai
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA
    Int J Oncol 20:891-6. 2002
    ....
  96. ncbi request reprint Cell cycle effects and caspase-dependent and independent death of HL-60 and Jurkat cells treated with the inhibitor of NF-kappaB parthenolide
    Piotr Pozarowski
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cell Cycle 2:377-83. 2003
    ..A caution, therefore, should be exercised in interpreting data of the experiments in which PRT is used with the intention to specifically prevent activation of NF-kappaB...
  97. ncbi request reprint Dual functionality of cyclooxygenase-2 as a regulator of tumor necrosis factor-mediated G1 shortening and nitric oxide-mediated inhibition of vascular smooth muscle cell proliferation
    Asifa Haider
    New York Medical College, Department of Pharmacology, Valhalla, NY 10595, USA
    Circulation 108:1015-21. 2003
    ..The present study demonstrates, however, that depending on prevailing conditions, COX-2-derived prostanoids may also inhibit VSMC proliferation...
  98. pmc Detection of DNA strand breaks by flow and laser scanning cytometry in studies of apoptosis and cell proliferation (DNA replication)
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, NY, USA
    Methods Mol Biol 314:81-93. 2006
    ....
  99. pmc Enforced adhesion of hematopoietic cells to culture dish induces endomitosis and polyploidy
    Xuan Huang
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cell Cycle 4:801-5. 2005
    ..The phenomenon offers novel experimental model for induction of polyploidy and provides alternative to cytocholasin B to prevent cytokinesis in the mutagenicity cytokinesis-blocked micronucleus (CBMN) assay...
  100. ncbi request reprint Effects of herbal preparation Equiguard on hormone-responsive and hormone-refractory prostate carcinoma cells: mechanistic studies
    Tze Chen Hsieh
    Department of Biochemistry and Molecular Biology, Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10595, USA
    Int J Oncol 20:681-9. 2002
    ....
  101. ncbi request reprint Simple, semiautomatic assay of cytostatic and cytotoxic effects of antitumor drugs by laser scanning cytometry: effects of the bis-intercalator WP631 on growth and cell cycle of T-24 cells
    Piotr Pozarowski
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cytometry A 57:113-9. 2004
    ..g., based on reduction of 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide, although rapid and convenient, do not provide information about individual cells or cell cycle effects and may be biased by growth imbalance...

Research Grants21

  1. PROBES FOR CYTOMETRY
    Zbigniew Darzynkiewicz; Fiscal Year: 2009
    ..abstract_text> ..
  2. PROBES FOR FLOW CYTOMETRY
    Zbigniew Darzynkiewicz; Fiscal Year: 1999
    ..it is expected that such a combined analysis, especially involving estimates of the G1 and G2 cyclins, will be of high prognostic value, and will be used during treatment, to monitor tumor response. ..
  3. PROBES FOR FLOW CYTOMETRY
    Zbigniew Darzynkiewicz; Fiscal Year: 1993
    ..This in turn will help assess the cellular features predicting susceptibility to particular drugs and aid in new drug design...
  4. PROBES FOR CYTOMETRY
    Zbigniew Darzynkiewicz; Fiscal Year: 2010
    ....