N J Cowan

Summary

Affiliation: New York University
Country: USA

Publications

  1. ncbi A chaperone with a hydrophilic surface
    N J Cowan
    Department of Biochemistry, New York University Medical Center, 550 First Avenue, New York, New York 10016, USA
    Nat Struct Biol 6:990-1. 1999
  2. ncbi Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin
    I E Vainberg
    Department of Biochemistry, New York University Medical Center, New York 10016, USA
    Cell 93:863-73. 1998
  3. pmc Tubulin subunits exist in an activated conformational state generated and maintained by protein cofactors
    G Tian
    Department of Biochemistry, New York University Medical Center 10016, USA
    J Cell Biol 138:821-32. 1997
  4. ncbi Pathway leading to correctly folded beta-tubulin
    G Tian
    Department of Biochemistry, New York University Medical Center, New York 10016, USA
    Cell 86:287-96. 1996
  5. ncbi A cytoplasmic chaperonin that catalyzes beta-actin folding
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    Cell 69:1043-50. 1992
  6. pmc Two cofactors and cytoplasmic chaperonin are required for the folding of alpha- and beta-tubulin
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    Mol Cell Biol 13:2478-85. 1993
  7. pmc ADP ribosylation factor-like protein 2 (Arl2) regulates the interaction of tubulin-folding cofactor D with native tubulin
    A Bhamidipati
    Department of Biochemistry, New York University Medical Center, New York, New York 10016, USA
    J Cell Biol 149:1087-96. 2000
  8. pmc A novel 205-kilodalton testis-specific serine/threonine protein kinase associated with microtubules of the spermatid manchette
    P D Walden
    Department of Biochemistry, New York University Medical Center, New York 10016
    Mol Cell Biol 13:7625-35. 1993
  9. pmc Chaperonin-mediated folding of actin and tubulin
    S A Lewis
    Department of Biochemistry, New York University Medical Center, New York 10016, USA
    J Cell Biol 132:1-4. 1996
  10. pmc A novel cochaperonin that modulates the ATPase activity of cytoplasmic chaperonin
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    J Cell Biol 125:989-96. 1994

Collaborators

  • H L Klein
  • H Rommelaere
  • Y Gao
  • P D Walden
  • R Melki
  • Y Huang
  • J O Thomas
  • William Welch
  • S A Lewis
  • G Tian
  • I E Vainberg
  • C Ampe
  • A Bhamidipati
  • W J Hansen
  • J Vandekerckhove
  • B Feierbach
  • T Stearns
  • J L Hall
  • M G Lee
  • D Wang
  • A Villasante
  • R D Burgoyne
  • M Noble
  • D W Cleveland
  • W J Rutter
  • M E Gilmartin
  • M W Kirschner
  • P Dobner
  • M A Cambray-Deakin
  • L Dudley
  • P Sherline
  • C Loomis
  • R J MacDonald
  • P Dolph
  • C D Wilde
  • M A Lopata
  • P R Dobner
  • D H Wang
  • S Sarkar

Detail Information

Publications27

  1. ncbi A chaperone with a hydrophilic surface
    N J Cowan
    Department of Biochemistry, New York University Medical Center, 550 First Avenue, New York, New York 10016, USA
    Nat Struct Biol 6:990-1. 1999
    ..The structure of the yeast homolog of cofactor A, Rbl2p, shows it to be a dimer with largely hydrophilic surfaces, reflecting the fact that it interacts with quasi-native, not unfolded, beta-tubulin...
  2. ncbi Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin
    I E Vainberg
    Department of Biochemistry, New York University Medical Center, New York 10016, USA
    Cell 93:863-73. 1998
    ..We show that by directing target proteins to chaperonin, prefoldin promotes folding in an environment in which there are many competing pathways for nonnative proteins...
  3. pmc Tubulin subunits exist in an activated conformational state generated and maintained by protein cofactors
    G Tian
    Department of Biochemistry, New York University Medical Center 10016, USA
    J Cell Biol 138:821-32. 1997
    ....
  4. ncbi Pathway leading to correctly folded beta-tubulin
    G Tian
    Department of Biochemistry, New York University Medical Center, New York 10016, USA
    Cell 86:287-96. 1996
    ..Sequence analysis identifies yeast homologs of cofactors D (cin1) and E (pac2), characterized by mutations that affect microtubule function...
  5. ncbi A cytoplasmic chaperonin that catalyzes beta-actin folding
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    Cell 69:1043-50. 1992
    ..The eukaryotic cytoplasm thus contains a chaperonin that is functionally analagous to its prokaryotic, mitochondrial, and chloroplastic counterparts...
  6. pmc Two cofactors and cytoplasmic chaperonin are required for the folding of alpha- and beta-tubulin
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    Mol Cell Biol 13:2478-85. 1993
    ..We show that actin and tubulins compete efficiently with one another for association with cytoplasmic chaperonin complexes. These data imply that actin and alpha- and beta-tubulin interact with the same site(s) on chaperonin complexes...
  7. pmc ADP ribosylation factor-like protein 2 (Arl2) regulates the interaction of tubulin-folding cofactor D with native tubulin
    A Bhamidipati
    Department of Biochemistry, New York University Medical Center, New York, New York 10016, USA
    J Cell Biol 149:1087-96. 2000
    ..These data establish a role for Arl2 in modulating the interaction of tubulin-folding cofactors with native tubulin in vivo...
  8. pmc A novel 205-kilodalton testis-specific serine/threonine protein kinase associated with microtubules of the spermatid manchette
    P D Walden
    Department of Biochemistry, New York University Medical Center, New York 10016
    Mol Cell Biol 13:7625-35. 1993
    ..The MAST205 protein complex may function to link the signal transduction pathway with the organization of manchette microtubules...
  9. pmc Chaperonin-mediated folding of actin and tubulin
    S A Lewis
    Department of Biochemistry, New York University Medical Center, New York 10016, USA
    J Cell Biol 132:1-4. 1996
  10. pmc A novel cochaperonin that modulates the ATPase activity of cytoplasmic chaperonin
    Y Gao
    Department of Biochemistry, New York University Medical Center, New York 10016
    J Cell Biol 125:989-96. 1994
    ..Moreover, while GroES functions as a heptameric ring, cofactor A behaves as a dimer. Thus, cofactor A is a novel cochaperonin that is structurally unrelated to GroES...
  11. ncbi Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein
    S A Lewis
    Department of Biochemistry, New York University Medical Center, NY 10016
    Science 242:936-9. 1988
    ..The occurrence of these repeats in tau protein suggests that these may be a general feature of microtubule binding proteins...
  12. pmc Prefoldin-nascent chain complexes in the folding of cytoskeletal proteins
    W J Hansen
    Surgical Research Laboratory, San Francisco General Hospital, Departments of Surgery, Medicine, and Physiology, University of California, San Francisco, California 94143, USA
    J Cell Biol 145:265-77. 1999
    ..We conclude that PFD functions by selectively targeting nascent actin and tubulin chains pending their transfer to CCT for final folding and/or assembly...
  13. pmc Eukaryotic cytosolic chaperonin contains t-complex polypeptide 1 and seven related subunits
    H Rommelaere
    Laboratory of Physiological Chemistry, University Ghent, Belgium
    Proc Natl Acad Sci U S A 90:11975-9. 1993
    ..We propose that a highly conserved region in these polypeptides and in other chaperonins of the cpn60 chaperone family participates in ATP binding...
  14. pmc The microtubule binding domain of microtubule-associated protein MAP1B contains a repeated sequence motif unrelated to that of MAP2 and tau
    M Noble
    Department of Biochemistry, New York University Medical Center 10016
    J Cell Biol 109:3367-76. 1989
    ..This region bears no sequence relationship to the microtubule binding domains of kinesin, MAP2, or tau...
  15. pmc Differential distribution of beta-tubulin isotypes in cerebellum
    R D Burgoyne
    Physiological Laboratory, University of Liverpool, UK
    EMBO J 7:2311-9. 1988
    ..However, the strict subcellular segregation of other MAPs in brain may be determined by other factors...
  16. pmc Brain-specific expression of MAP2 detected using a cloned cDNA probe
    S A Lewis
    J Cell Biol 102:2098-105. 1986
    ..Thus, there is significant interspecies divergence of MAP2 sequences. The implications of the above observations are discussed in relationship to the potential biological function of MAP2...
  17. pmc Complete sequence of a cDNA encoding mouse MAP2
    D Wang
    Department of Biochemistry, NYU Medical Center 10016
    Nucleic Acids Res 16:11369-70. 1988
  18. pmc Six mouse alpha-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes
    A Villasante
    Mol Cell Biol 6:2409-19. 1986
    ..Patterns of developmental expression of the various alpha-tubulin isotypes are presented. Our data support the view that individual tubulin isotypes are capable of conferring functional specificity on different kinds of microtubules...
  19. ncbi Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes
    D W Cleveland
    Cell 20:95-105. 1980
  20. pmc Identification of two human beta-tubulin isotypes
    J L Hall
    Mol Cell Biol 3:854-62. 1983
    ..Thus, at least two human beta-tubulin genes, each specifying a distinct isotype, are expressed in HeLa cells, and the 2.6-kilobase mRNA band is a composite of at least two comigrating beta-tubulin mRNAs...
  21. pmc The mammalian beta-tubulin repertoire: hematopoietic expression of a novel, heterologous beta-tubulin isotype
    D Wang
    J Cell Biol 103:1903-10. 1986
    ..We predict that the M beta 1 isotype is functionally specialized for assembly into the mammalian marginal band...
  22. pmc Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions
    N J Cowan
    Mol Cell Biol 3:1738-45. 1983
    ....
  23. pmc Sequence of an expressed human beta-tubulin gene containing ten Alu family members
    M G Lee
    Nucleic Acids Res 12:5823-36. 1984
    ..The ten Alu sequences are substantially divergent, both from each other and from an Alu consensus sequence, and several contain deletions of up to half the entire sequence...
  24. ncbi Three expressed sequences within the human beta-tubulin multigene family each define a distinct isotype
    S A Lewis
    J Mol Biol 182:11-20. 1985
    ..The overall composition of the multigene family and the evolutionary relationships among its various members are discussed...
  25. pmc Five mouse tubulin isotypes and their regulated expression during development
    S A Lewis
    J Cell Biol 101:852-61. 1985
    ..Tissue-specific development changes in the abundance of tubulin isotype-specific mRNAs are discussed in relationship to organogenesis in the mouse...
  26. pmc Structural features and restricted expression of a human alpha-tubulin gene
    J L Hall
    Nucleic Acids Res 13:207-23. 1985
    ..The data presented suggest that the b alpha 1 gene encodes a prominent neuronal and glial alpha-tubulin and that b alpha 1 expression is a function of the differentiated state of these cells...
  27. ncbi Evolutionary history of a multigene family: an expressed human beta-tubulin gene and three processed pseudogenes
    M G Lee
    Cell 33:477-87. 1983
    ..We anticipate that, in multigene families where germ-line expression occurs, a significant portion of sequences will be accounted for by pseudogenes generated via an RNA intermediate...