S Chong

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. pmc In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme
    Haruichi Asahara
    New England Biolabs Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 38:e141. 2010
  2. pmc Reconstitution of translation from Thermus thermophilus reveals a minimal set of components sufficient for protein synthesis at high temperatures and functional conservation of modern and ancient translation components
    Ying Zhou
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 40:7932-45. 2012
  3. pmc A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based
    Jason R Hillebrecht
    New England Biolabs, 240 County Road, Ipswich, MA 01938, USA
    BMC Biotechnol 8:58. 2008
  4. pmc Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
    S Chong
    New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 26:5109-15. 1998
  5. ncbi request reprint Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
    S Chong
    New England Biolabs, Beverly, MA 01915, USA
    Gene 192:271-81. 1997
  6. ncbi request reprint Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins
    A Zhang
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Gene 275:241-52. 2001
  7. ncbi request reprint Intein-mediated rapid purification of Cre recombinase
    E J Cantor
    New England Biolabs, Inc, 32 Tozer Road, Beverly, Massachusetts 01915, USA
    Protein Expr Purif 22:135-40. 2001
  8. ncbi request reprint Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation
    S Mathys
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Gene 231:1-13. 1999

Research Grants

Collaborators

Detail Information

Publications8

  1. pmc In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme
    Haruichi Asahara
    New England Biolabs Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 38:e141. 2010
    ....
  2. pmc Reconstitution of translation from Thermus thermophilus reveals a minimal set of components sufficient for protein synthesis at high temperatures and functional conservation of modern and ancient translation components
    Ying Zhou
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 40:7932-45. 2012
    ..thermophilus. Because it contains significantly reduced nucleases and proteases, this reconstituted thermostable cell-free protein synthesis system may enable in vitro engineering of proteins with improved thermostability...
  3. pmc A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based
    Jason R Hillebrecht
    New England Biolabs, 240 County Road, Ipswich, MA 01938, USA
    BMC Biotechnol 8:58. 2008
    ..A detailed comparison of in vitro protein synthesis in different cell-free systems may provide insights to their biological differences and guidelines for their applications...
  4. pmc Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
    S Chong
    New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 26:5109-15. 1998
    ..We have constructed general cloning vectors and demonstrated single-column purification of several proteins. In addition, we discuss several factors that may affect the C-terminal peptide bond cleavage activity...
  5. ncbi request reprint Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
    S Chong
    New England Biolabs, Beverly, MA 01915, USA
    Gene 192:271-81. 1997
    ..No exogenous proteolytic cleavage is needed. Furthermore, using this procedure, the purified free target protein can be specifically labeled at its C-terminus...
  6. ncbi request reprint Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins
    A Zhang
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Gene 275:241-52. 2001
    ....
  7. ncbi request reprint Intein-mediated rapid purification of Cre recombinase
    E J Cantor
    New England Biolabs, Inc, 32 Tozer Road, Beverly, Massachusetts 01915, USA
    Protein Expr Purif 22:135-40. 2001
    ..The activity of the purified Cre was determined in an in vitro assay system and was found to remain stable over a period of more than 6 months...
  8. ncbi request reprint Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation
    S Mathys
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Gene 231:1-13. 1999
    ..The intein-generated proteins were joined together through a native peptide bond. This intein-mediated protein ligation approach opens up novel routes in protein engineering...

Research Grants1

  1. Prevention of Recombinant Protein Aggregation in E.coli
    Shaorong Chong; Fiscal Year: 2005
    ..As a long-term goal, this project aims to provide insights into the mechanism of the aggregation by these disease-related proteins and the means to prevent aggregation. ..