S Chong

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. ncbi A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based
    Jason R Hillebrecht
    New England Biolabs, 240 County Road, Ipswich, MA 01938, USA
    BMC Biotechnol 8:58. 2008
  2. ncbi Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
    S Chong
    New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 26:5109-15. 1998
  3. ncbi Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
    S Chong
    New England Biolabs, Beverly, MA 01915, USA
    Gene 192:271-81. 1997
  4. ncbi Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins
    A Zhang
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Gene 275:241-52. 2001
  5. ncbi Intein-mediated rapid purification of Cre recombinase
    E J Cantor
    New England Biolabs, Inc, 32 Tozer Road, Beverly, Massachusetts 01915, USA
    Protein Expr Purif 22:135-40. 2001
  6. ncbi Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation
    S Mathys
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Gene 231:1-13. 1999

Research Grants

  1. Prevention of Recombinant Protein Aggregation in E.coli
    Shaorong Chong; Fiscal Year: 2005

Collaborators

Detail Information

Publications6

  1. ncbi A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based
    Jason R Hillebrecht
    New England Biolabs, 240 County Road, Ipswich, MA 01938, USA
    BMC Biotechnol 8:58. 2008
    ..A detailed comparison of in vitro protein synthesis in different cell-free systems may provide insights to their biological differences and guidelines for their applications...
  2. ncbi Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
    S Chong
    New England Biolabs Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 26:5109-15. 1998
    ..We have constructed general cloning vectors and demonstrated single-column purification of several proteins. In addition, we discuss several factors that may affect the C-terminal peptide bond cleavage activity...
  3. ncbi Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
    S Chong
    New England Biolabs, Beverly, MA 01915, USA
    Gene 192:271-81. 1997
    ..No exogenous proteolytic cleavage is needed. Furthermore, using this procedure, the purified free target protein can be specifically labeled at its C-terminus...
  4. ncbi Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins
    A Zhang
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Gene 275:241-52. 2001
    ....
  5. ncbi Intein-mediated rapid purification of Cre recombinase
    E J Cantor
    New England Biolabs, Inc, 32 Tozer Road, Beverly, Massachusetts 01915, USA
    Protein Expr Purif 22:135-40. 2001
    ..The activity of the purified Cre was determined in an in vitro assay system and was found to remain stable over a period of more than 6 months...
  6. ncbi Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation
    S Mathys
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Gene 231:1-13. 1999
    ..The intein-generated proteins were joined together through a native peptide bond. This intein-mediated protein ligation approach opens up novel routes in protein engineering...

Research Grants1

  1. Prevention of Recombinant Protein Aggregation in E.coli
    Shaorong Chong; Fiscal Year: 2005
    ..As a long-term goal, this project aims to provide insights into the mechanism of the aggregation by these disease-related proteins and the means to prevent aggregation. ..