Siu Hong Chan

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. pmc Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity
    Siu Hong Chan
    New England Biolabs, Inc Ipswich, MA 01938, USA
    Nucleic Acids Res 39:1-18. 2011
  2. pmc Catalytic domain of restriction endonuclease BmrI as a cleavage module for engineering endonucleases with novel substrate specificities
    Siu Hong Chan
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:6238-48. 2007
  3. pmc Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
    Priscilla Hiu Mei Too
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 38:1294-303. 2010
  4. ncbi request reprint Cloning of Nt.CviQII nicking endonuclease and its cognate methyltransferase: M.CviQII methylates AG sequences
    Siu Hong Chan
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 49:138-50. 2006
  5. pmc Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA
    Penghua Zhang
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 69:226-34. 2010
  6. pmc Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI
    Shuang yong Xu
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:4608-18. 2007
  7. pmc Cloning of CviPII nicking and modification system from chlorella virus NYs-1 and application of Nt.CviPII in random DNA amplification
    Siu Hong Chan
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 32:6187-99. 2004
  8. pmc Increasing cleavage specificity and activity of restriction endonuclease KpnI
    Kommireddy Vasu
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India, Department of Chemistry, New York University, NY, USA, Department of Physics, Free University Berlin, Arnimallee 14, 14195 Berlin, Germany, New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA and Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560 064, India
    Nucleic Acids Res 41:9812-24. 2013
  9. pmc Expression and purification of BmrI restriction endonuclease and its N-terminal cleavage domain variants
    Yongming Bao
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 58:42-52. 2008
  10. pmc A type IV modification-dependent restriction enzyme SauUSI from Staphylococcus aureus subsp. aureus USA300
    Shuang yong Xu
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 39:5597-610. 2011

Collaborators

  • Shuang yong Xu
  • James C Samuelson
  • Yu Zheng
  • David D Dunigan
  • Petra Imhof
  • Priscilla Hiu Mei Too
  • Penghua Zhang
  • Kommireddy Vasu
  • Zhenyu Zhu
  • Alexey Fomenkov
  • Yongming Bao
  • Valakunja Nagaraja
  • Easa Nagamalleswari
  • Mai Zahran
  • Stephanie Doucette
  • Dan Forrest
  • Timothy M Schramm
  • David C Schwartz
  • Stefan Bäckström
  • Tamas Vincze
  • Konstantinos D Potamousis
  • Romas Vaisvila
  • Suzanne Sweeney
  • Beth Ann Cantin
  • Keith Lunnen
  • Lauren Higgins
  • Laurie Mazzola
  • Sophie Laget
  • Vernissia Tam

Detail Information

Publications11

  1. pmc Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity
    Siu Hong Chan
    New England Biolabs, Inc Ipswich, MA 01938, USA
    Nucleic Acids Res 39:1-18. 2011
    ..This review aims at providing an overview of the cleavage mechanisms of Types IIS and IIA REases and LAGLIDADG HEases, the engineering of their nicking variants, and the applications of NEases and nicking HEases...
  2. pmc Catalytic domain of restriction endonuclease BmrI as a cleavage module for engineering endonucleases with novel substrate specificities
    Siu Hong Chan
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:6238-48. 2007
    ..The resulting endonucleases will be useful in vitro and in vivo to create ds breaks at specific sites and generate deletions...
  3. pmc Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
    Priscilla Hiu Mei Too
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 38:1294-303. 2010
    ....
  4. ncbi request reprint Cloning of Nt.CviQII nicking endonuclease and its cognate methyltransferase: M.CviQII methylates AG sequences
    Siu Hong Chan
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 49:138-50. 2006
    ..To our knowledge, M.CviQII is the first adenine methyltransferase that recognizes a dinucleotide. Therefore, M.CviQII may be a useful reagent for blocking endonuclease cleavage when restriction sites overlap with AG sequences...
  5. pmc Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA
    Penghua Zhang
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 69:226-34. 2010
    ..Restriction mapping and run-off DNA sequencing of digested products from the partially purified enzyme indicated that it is an EarI isoschizomer with 6-bp recognition, which we named CatHI (CTCTTC N1/N4)...
  6. pmc Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI
    Shuang yong Xu
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:4608-18. 2007
    ..Top-strand specific nicking variants, Nt.BsrDI and Nt.BtsI, were successfully engineered by combining the catalytic-deficient B subunit with wild-type A subunit...
  7. pmc Cloning of CviPII nicking and modification system from chlorella virus NYs-1 and application of Nt.CviPII in random DNA amplification
    Siu Hong Chan
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Nucleic Acids Res 32:6187-99. 2004
    ..Nt.CviPII was used in combination with Bst DNA polymerase I large fragment to rapidly amplify anonymous DNA from genomic DNA or from a single bacterial colony...
  8. pmc Increasing cleavage specificity and activity of restriction endonuclease KpnI
    Kommireddy Vasu
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India, Department of Chemistry, New York University, NY, USA, Department of Physics, Free University Berlin, Arnimallee 14, 14195 Berlin, Germany, New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA and Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560 064, India
    Nucleic Acids Res 41:9812-24. 2013
    ..Our results show that active site plasticity in coordinating different metal ions is related to KpnI promiscuous activity, and tinkering the metal ion coordination is a plausible way to reduce promiscuous activity of metalloenzymes. ..
  9. pmc Expression and purification of BmrI restriction endonuclease and its N-terminal cleavage domain variants
    Yongming Bao
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 58:42-52. 2008
    ..The Cys-containing BmrI192 and BmrI200 nuclease variants may be useful for coupling to other DNA binding elements such as synthetic zinc fingers, thio-containing locked nucleic acids (LNA) or peptide nucleic acids (PNA)...
  10. pmc A type IV modification-dependent restriction enzyme SauUSI from Staphylococcus aureus subsp. aureus USA300
    Shuang yong Xu
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 39:5597-610. 2011
    ..aureus strain SA564, and in restricting phage λ infection when the endonuclease is expressed in E. coli...
  11. doi request reprint Targeting DNA 5mCpG sites with chimeric endonucleases
    Alexey Fomenkov
    New England Biolabs, Inc, 240 County Road Ipswich, MA 01938 2723, USA
    Anal Biochem 381:135-41. 2008
    ..Such (5)mCpG-specific endonucleases will be useful to study CpG island modification of the regulatory regions of tumor suppressor genes, and for the construction of cell-specific and tumor-specific modified CpG island databases...