Siu Hong Chan

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. ncbi Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity
    Siu Hong Chan
    New England Biolabs, Inc Ipswich, MA 01938, USA
    Nucleic Acids Res 39:1-18. 2011
  2. ncbi Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA
    Penghua Zhang
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 69:226-34. 2010
  3. ncbi Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
    Priscilla Hiu Mei Too
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 38:1294-303. 2010
  4. ncbi Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI
    Shuang yong Xu
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:4608-18. 2007
  5. ncbi A type IV modification-dependent restriction enzyme SauUSI from Staphylococcus aureus subsp. aureus USA300
    Shuang yong Xu
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 39:5597-610. 2011
  6. ncbi Catalytic domain of restriction endonuclease BmrI as a cleavage module for engineering endonucleases with novel substrate specificities
    Siu Hong Chan
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:6238-48. 2007
  7. ncbi Expression and purification of BmrI restriction endonuclease and its N-terminal cleavage domain variants
    Yongming Bao
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 58:42-52. 2008
  8. ncbi Targeting DNA 5mCpG sites with chimeric endonucleases
    Alexey Fomenkov
    New England Biolabs, Inc, 240 County Road Ipswich, MA 01938 2723, USA
    Anal Biochem 381:135-41. 2008

Collaborators

  • Shuang yong Xu
  • James C Samuelson
  • Yu Zheng
  • Priscilla Hiu Mei Too
  • Penghua Zhang
  • Alexey Fomenkov
  • Yongming Bao
  • David C Schwartz
  • Stefan Bäckström
  • Stephanie Doucette
  • Dan Forrest
  • Timothy M Schramm
  • Tamas Vincze
  • Zhenyu Zhu
  • Konstantinos D Potamousis
  • Beth Ann Cantin
  • Keith Lunnen
  • Lauren Higgins
  • Laurie Mazzola
  • Sophie Laget
  • Priscilla Hiu-Mei Too
  • Vernissia Tam
  • Suzanne Sweeney
  • Romas Vaisvila

Detail Information

Publications8

  1. ncbi Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity
    Siu Hong Chan
    New England Biolabs, Inc Ipswich, MA 01938, USA
    Nucleic Acids Res 39:1-18. 2011
    ..This review aims at providing an overview of the cleavage mechanisms of Types IIS and IIA REases and LAGLIDADG HEases, the engineering of their nicking variants, and the applications of NEases and nicking HEases...
  2. ncbi Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA
    Penghua Zhang
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 69:226-34. 2010
    ..Restriction mapping and run-off DNA sequencing of digested products from the partially purified enzyme indicated that it is an EarI isoschizomer with 6-bp recognition, which we named CatHI (CTCTTC N1/N4)...
  3. ncbi Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
    Priscilla Hiu Mei Too
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 38:1294-303. 2010
    ....
  4. ncbi Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI
    Shuang yong Xu
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:4608-18. 2007
    ..Top-strand specific nicking variants, Nt.BsrDI and Nt.BtsI, were successfully engineered by combining the catalytic-deficient B subunit with wild-type A subunit...
  5. ncbi A type IV modification-dependent restriction enzyme SauUSI from Staphylococcus aureus subsp. aureus USA300
    Shuang yong Xu
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 39:5597-610. 2011
    ..aureus strain SA564, and in restricting phage λ infection when the endonuclease is expressed in E. coli...
  6. ncbi Catalytic domain of restriction endonuclease BmrI as a cleavage module for engineering endonucleases with novel substrate specificities
    Siu Hong Chan
    New England Biolabs, Inc 240 County Road, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:6238-48. 2007
    ..The resulting endonucleases will be useful in vitro and in vivo to create ds breaks at specific sites and generate deletions...
  7. ncbi Expression and purification of BmrI restriction endonuclease and its N-terminal cleavage domain variants
    Yongming Bao
    New England Biolabs, Inc, 240 County Road, Ipswich, MA 01938, USA
    Protein Expr Purif 58:42-52. 2008
    ..The Cys-containing BmrI192 and BmrI200 nuclease variants may be useful for coupling to other DNA binding elements such as synthetic zinc fingers, thio-containing locked nucleic acids (LNA) or peptide nucleic acids (PNA)...
  8. ncbi Targeting DNA 5mCpG sites with chimeric endonucleases
    Alexey Fomenkov
    New England Biolabs, Inc, 240 County Road Ipswich, MA 01938 2723, USA
    Anal Biochem 381:135-41. 2008
    ..Such (5)mCpG-specific endonucleases will be useful to study CpG island modification of the regulatory regions of tumor suppressor genes, and for the construction of cell-specific and tumor-specific modified CpG island databases...