Jurate Bitinaite

Summary

Affiliation: New England Biolabs
Country: USA

Publications

  1. ncbi Self-generated DNA termini relax the specificity of SgrAI restriction endonuclease
    Jurate Bitinaite
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Proc Natl Acad Sci U S A 99:1164-9. 2002
  2. ncbi USER friendly DNA engineering and cloning method by uracil excision
    Jurate Bitinaite
    New England Biolabs, Inc, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:1992-2002. 2007
  3. ncbi Biochemical characterization of recombinant β-glucosyltransferase and analysis of global 5-hydroxymethylcytosine in unique genomes
    Jolyon Terragni
    New England Biolabs, Inc, Ipswich, Massachusetts 01938, United States
    Biochemistry 51:1009-19. 2012
  4. ncbi A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
    Richard J Roberts
    New England Biolabs, Beverly, MA 01915, USA
    Nucleic Acids Res 31:1805-12. 2003
  5. ncbi Kinetic analysis of the coordinated interaction of SgrAI restriction endonuclease with different DNA targets
    Kamini Hingorani-Varma
    New England Biolabs Inc, Beverly, Massachusetts 01915, USA
    J Biol Chem 278:40392-9. 2003
  6. ncbi DNA cloning and engineering by uracil excision
    Jurate Bitinaite
    New England Biolabs, Ipswich, Massachusetts, USA
    Curr Protoc Mol Biol . 2009
  7. ncbi Gene synthesis by assembly of deoxyuridine-containing oligonucleotides
    Romualdas Vaisvila
    New England Biolabs, Inc, Ipswich, MA, USA
    Methods Mol Biol 978:165-71. 2013
  8. ncbi Tissue-specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genomes
    Shannon Morey Kinney
    New England Biolabs, Inc, Ipswich, Massachusetts 01938, USA
    J Biol Chem 286:24685-93. 2011
  9. ncbi DNA distortion and specificity in a sequence-specific endonuclease
    Andrea C Babic
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721, USA
    J Mol Biol 383:186-204. 2008
  10. ncbi Alteration of sequence specificity of the type II restriction endonuclease HincII through an indirect readout mechanism
    Hemant K Joshi
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    J Biol Chem 281:23852-69. 2006

Collaborators

Detail Information

Publications11

  1. ncbi Self-generated DNA termini relax the specificity of SgrAI restriction endonuclease
    Jurate Bitinaite
    New England Biolabs, Inc, 32 Tozer Road, Beverly, MA 01915, USA
    Proc Natl Acad Sci U S A 99:1164-9. 2002
    ..Based on our work and previous reports, a pathway of DNA binding and cleavage by the SgrAI restriction endonuclease is proposed...
  2. ncbi USER friendly DNA engineering and cloning method by uracil excision
    Jurate Bitinaite
    New England Biolabs, Inc, Ipswich, MA 01938, USA
    Nucleic Acids Res 35:1992-2002. 2007
    ..By varying the design of the PCR primers, the protocol is easily adapted to perform one or more simultaneous DNA manipulations such as directional cloning, site-specific mutagenesis, sequence insertion or deletion and sequence assembly...
  3. ncbi Biochemical characterization of recombinant β-glucosyltransferase and analysis of global 5-hydroxymethylcytosine in unique genomes
    Jolyon Terragni
    New England Biolabs, Inc, Ipswich, Massachusetts 01938, United States
    Biochemistry 51:1009-19. 2012
    ..A comparison between cancer and healthy tissue genomes suggested a lower percentage of 5-hmC in cancer, which may reflect the global hypomethylation of 5-mC observed during oncogenesis...
  4. ncbi A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
    Richard J Roberts
    New England Biolabs, Beverly, MA 01915, USA
    Nucleic Acids Res 31:1805-12. 2003
    ..It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes...
  5. ncbi Kinetic analysis of the coordinated interaction of SgrAI restriction endonuclease with different DNA targets
    Kamini Hingorani-Varma
    New England Biolabs Inc, Beverly, Massachusetts 01915, USA
    J Biol Chem 278:40392-9. 2003
    ..The free subunits of SgrAI have the flexibility to bind different target sites and, consequently, assemble into various catalytically active complexes, which differ in their catalytic efficiencies...
  6. ncbi DNA cloning and engineering by uracil excision
    Jurate Bitinaite
    New England Biolabs, Ipswich, Massachusetts, USA
    Curr Protoc Mol Biol . 2009
    ..The use of a commercially available cloning vector and the preparation of custom vectors are also presented...
  7. ncbi Gene synthesis by assembly of deoxyuridine-containing oligonucleotides
    Romualdas Vaisvila
    New England Biolabs, Inc, Ipswich, MA, USA
    Methods Mol Biol 978:165-71. 2013
    ..The method consists of (1) synthesis of building blocks up to 500 bp; (2) assembly of genes up to 3 kb; (3) error correction reassembly; and (4) assembly of operons up to 15 kb if needed...
  8. ncbi Tissue-specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genomes
    Shannon Morey Kinney
    New England Biolabs, Inc, Ipswich, Massachusetts 01938, USA
    J Biol Chem 286:24685-93. 2011
    ..Finally, using a quantitative PCR approach, we established that a portion of VANGL1 and EGFR gene body methylation in human tissue DNA samples is indeed hydroxymethylation...
  9. ncbi DNA distortion and specificity in a sequence-specific endonuclease
    Andrea C Babic
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721, USA
    J Mol Biol 383:186-204. 2008
    ..The various complexes are fit into a model describing the different conformations of the DNA-bound enzyme and show how DNA conformational energetics determine DNA-cleavage rates by the Q138F HincII enzyme...
  10. ncbi Alteration of sequence specificity of the type II restriction endonuclease HincII through an indirect readout mechanism
    Hemant K Joshi
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    J Biol Chem 281:23852-69. 2006
    ..Analysis of these structures and the effect of Ca2+ binding on the protein-DNA interface illuminates the origin of the altered specificity by the mutation Q138F in the HincII enzyme...
  11. ncbi The structure of SgrAI bound to DNA; recognition of an 8 base pair target
    Pete W Dunten
    Stanford Synchrotron Radiation Laboratory, Stanford University, Menlo Park, CA 94025, USA
    Nucleic Acids Res 36:5405-16. 2008
    ..Recognition of the outer GC base pairs occurs through a single contact to the G from an arginine side chain located in a region unique to SgrAI...