Subhasis Biswas

Summary

Affiliation: New Jersey
Country: USA

Publications

  1. pmc Thermodynamic analysis of DNA binding by a Bacillus single stranded DNA binding protein
    Esther E Biswas-Fiss
    Department of Molecular Biology, School of Osteopathic Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, NJ 08084, USA
    BMC Biochem 13:10. 2012
  2. ncbi request reprint Quantitative analysis of binding of single-stranded DNA by Escherichia coli DnaB helicase and the DnaB x DnaC complex
    Subhasis B Biswas
    Department of Molecular Biology, School of Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 45:11505-13. 2006
  3. ncbi request reprint Control of ATP-dependent binding of Saccharomyces cerevisiae origin recognition complex to autonomously replicating DNA sequences
    Subhasis B Biswas
    Department of Molecular Biology, Graduate School of Biomedical Sciences, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08043, USA
    Cell Cycle 4:494-500. 2005
  4. ncbi request reprint Purification and characterization of DNA polymerase alpha-associated replication protein A-dependent yeast DNA helicase A
    S B Biswas
    Department of Molecular Biology, Science Center, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 36:13270-6. 1997
  5. pmc Modulation of enzymatic activities of Escherichia coli DnaB helicase by single-stranded DNA-binding proteins
    Esther E Biswas
    Department of Molecular Biology and School of Osteopathic Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, 2 Medical Center Drive, Stratford, NJ 08084, USA
    Nucleic Acids Res 30:2809-16. 2002
  6. ncbi request reprint Conformational dynamics of DnaB helicase upon DNA and nucleotide binding: analysis by intrinsic tryptophan fluorescence quenching
    Stephen Flowers
    Department of Molecular Biology, School of Osteopathic Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 42:1910-21. 2003
  7. ncbi request reprint Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis
    Atanaska V Mitkova
    Department of Molecular Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    J Biol Chem 278:52253-61. 2003
  8. ncbi request reprint Affinity and sequence specificity of DNA binding and site selection for primer synthesis by Escherichia coli primase
    Sujata Khopde
    Department of Molecular Biology, School of Osteopathic Medicine, and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford 08084, USA
    Biochemistry 41:14820-30. 2002
  9. pmc An essential DnaB helicase of Bacillus anthracis: identification, characterization, and mechanism of action
    Esther E Biswas
    Department of Molecular Biology, UMDNJ SOM, 2 Medical Center Dr, Stratford, NJ 08084, USA
    J Bacteriol 191:249-60. 2009
  10. ncbi request reprint Cell cycle specific plasmid DNA replication in the nuclear extract of Saccharomyces cerevisiae: modulation by replication protein A and proliferating cell nuclear antigen
    Atanaska V Mitkova
    Department of Molecular Biology, School of Osteopathic Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 41:5255-65. 2002

Research Grants

Collaborators

Detail Information

Publications16

  1. pmc Thermodynamic analysis of DNA binding by a Bacillus single stranded DNA binding protein
    Esther E Biswas-Fiss
    Department of Molecular Biology, School of Osteopathic Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, NJ 08084, USA
    BMC Biochem 13:10. 2012
    ..Single-stranded DNA binding proteins (SSB) are essential for DNA replication, repair, and recombination in all organisms. SSB works in concert with a variety of DNA metabolizing enzymes such as DNA polymerase...
  2. ncbi request reprint Quantitative analysis of binding of single-stranded DNA by Escherichia coli DnaB helicase and the DnaB x DnaC complex
    Subhasis B Biswas
    Department of Molecular Biology, School of Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 45:11505-13. 2006
    ..In summary, our results suggest that in the presence of ATP in vivo, the DnaB6 x DnaC6 complex should be more efficient in binding DNA as well as in loading DnaB onto the ssDNA than DnaB helicase itself...
  3. ncbi request reprint Control of ATP-dependent binding of Saccharomyces cerevisiae origin recognition complex to autonomously replicating DNA sequences
    Subhasis B Biswas
    Department of Molecular Biology, Graduate School of Biomedical Sciences, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08043, USA
    Cell Cycle 4:494-500. 2005
    ..Thus, the consensus ARS sequence in element A appeared to play a pivotal role in the ATP-dependent binding of ORC to ARS1 and likely in other ARSs or origins of DNA replication...
  4. ncbi request reprint Purification and characterization of DNA polymerase alpha-associated replication protein A-dependent yeast DNA helicase A
    S B Biswas
    Department of Molecular Biology, Science Center, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 36:13270-6. 1997
    ....
  5. pmc Modulation of enzymatic activities of Escherichia coli DnaB helicase by single-stranded DNA-binding proteins
    Esther E Biswas
    Department of Molecular Biology and School of Osteopathic Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, 2 Medical Center Drive, Stratford, NJ 08084, USA
    Nucleic Acids Res 30:2809-16. 2002
    ..Thus, E.coli SSB appears to coordinate and couple the ATPase activity to the DNA helicase activity by suppressing unproductive ATP hydrolysis by DnaB helicase...
  6. ncbi request reprint Conformational dynamics of DnaB helicase upon DNA and nucleotide binding: analysis by intrinsic tryptophan fluorescence quenching
    Stephen Flowers
    Department of Molecular Biology, School of Osteopathic Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 42:1910-21. 2003
    ..This is the first reported elucidation of the putative role of domain alpha, which is essential for DNA helicase action. We have correlated these results with partial structural models of alpha, beta, and gamma domains..
  7. ncbi request reprint Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis
    Atanaska V Mitkova
    Department of Molecular Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    J Biol Chem 278:52253-61. 2003
    ..Therefore, interactions of the DnaB-primase complex with other replication factors might be critical for determining the physiological length of the RNA primers in vivo and the overall kinetics of primer synthesis...
  8. ncbi request reprint Affinity and sequence specificity of DNA binding and site selection for primer synthesis by Escherichia coli primase
    Sujata Khopde
    Department of Molecular Biology, School of Osteopathic Medicine, and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford 08084, USA
    Biochemistry 41:14820-30. 2002
    ..This sequence preference of primase may likely be responsible for the initiation of primer synthesis in the CTG triplet sites in the E. coli lagging strand as well as in the origin of replication of bacteriophage G4...
  9. pmc An essential DnaB helicase of Bacillus anthracis: identification, characterization, and mechanism of action
    Esther E Biswas
    Department of Molecular Biology, UMDNJ SOM, 2 Medical Center Dr, Stratford, NJ 08084, USA
    J Bacteriol 191:249-60. 2009
    ..Therefore, DnaB(BA) has an attenuated ATPase activity and a highly active DNA helicase activity. Based on the ratio of DNA helicase and ATPase activities, DnaB(BA) is highly efficient in DNA unwinding and its coupling to ATP consumption...
  10. ncbi request reprint Cell cycle specific plasmid DNA replication in the nuclear extract of Saccharomyces cerevisiae: modulation by replication protein A and proliferating cell nuclear antigen
    Atanaska V Mitkova
    Department of Molecular Biology, School of Osteopathic Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 41:5255-65. 2002
    ..Results presented here demonstrate that both RPA and PCNA are cell cycle-independent in their ability to stimulate in vitro plasmid DNA replication, whereas replication factors in the polymerase fractions are strictly S-phase dependent...
  11. ncbi request reprint Modulation of DNA synthesis in Saccharomyces cerevisiae nuclear extract by DNA polymerases and the origin recognition complex
    Atanaska V Mitkova
    Department of Molecular Biology, GSBS and SOM, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    J Biol Chem 280:6285-92. 2005
    ..In addition, we also report here a novel finding that purified recombinant six subunit ORC significantly stimulated the DNA synthesis on a supercoiled plasmid DNA template containing an autonomously replicating sequence, ARS1...
  12. ncbi request reprint The Mcm467 complex of Saccharomyces cerevisiae is preferentially activated by autonomously replicating DNA sequences
    Esther E Biswas-Fiss
    Department of Molecular Biology, School of Osteopathic Medicine, Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 44:2916-25. 2005
    ..Our results also indicate that the yeast replication protein A stimulated the ATPase activity of the Mcm467 complex...
  13. pmc Subunit interactions in the assembly of Saccharomyces cerevisiae DNA polymerase alpha
    Subhasis B Biswas
    Department of Molecular Biology, School of Medicine and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, NJ 08084, USA
    Nucleic Acids Res 31:2056-65. 2003
    ..A strong interaction between p167 and p62 would probably require the presence of either p79 or p48 in the complex...
  14. pmc Mechanisms of DNA binding and regulation of Bacillus anthracis DNA primase
    Subhasis B Biswas
    Department of Molecular Biology, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    Biochemistry 48:7373-82. 2009
    ..anthracis and E. coli; however, between DnaB proteins derived from these two organisms, only the homologous DNA helicase (DnaB(BA)) acted as a positive effector of primer synthesis...
  15. pmc Quantitative analysis of nucleotide modulation of DNA binding by DnaC protein of Escherichia coli
    Subhasis B Biswas
    Department of Molecular Biology, School of Medicine, Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Stratford, NJ 08084, USA
    Biochem J 379:553-62. 2004
    ..Based on the thermodynamic data provided in this study, we have proposed a mechanism of DnaB loading on to ssDNA by DnaC protein...
  16. ncbi request reprint Biochemical defects in retina-specific human ATP binding cassette transporter nucleotide binding domain 1 mutants associated with macular degeneration
    Tatiana Suarez
    Department of Laboratory Sciences, Program in Biotechnology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 277:21759-67. 2002
    ..We have correlated the dissociation constant (K(D)) and the rates of nucleotide hydrolysis (V(max)) of NBD1 and its mutants with the available genetic data for these mutations...

Research Grants7

  1. MECHANISM OF DNA-PROTEIN INTERACTION IN DNA REPLICATION
    Subhasis Biswas; Fiscal Year: 2002
    ..Biochemical studies of purified helicase A will characterize the nucleotidase and helicase activities of this protein. ..