Research Topics
| VIVIAN M BELLOFATTOSummaryAffiliation: New Jersey Country: USA Publications
Research Grants
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Detail Information
Publications
Transcription and mRNA stability: parental guidance suggestedVivian Bellofatto
Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
Cell 147:1438-9. 2011..and Trcek et al. provide evidence that these two processes are integrated. They show that transcription factors and DNA promoters can directly influence the relative stability of transcripts that they produce...- Pyrimidine transport activities in trypanosomesVivian Bellofatto
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA
Trends Parasitol 23:187-9; discussion 190. 2007..The work by Gudin et al. characterizes the dynamics of these transporters and points to further areas for future genetic and therapeutic experiments... 
The non-canonical CTD of RNAP-II is essential for productive RNA synthesis in Trypanosoma bruceiAnish Das
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey, United States of America
PLoS ONE 4:e6959. 2009..RNAP-II with PsiCTD truncations resulted in abortive initiation of transcription. These data establish that non-canonical CTDs play an important role in gene expression...- Trypanosomal TBP functions with the multisubunit transcription factor tSNAP to direct spliced-leader RNA gene expressionAnish Das
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark, 07103, USA
Mol Cell Biol 25:7314-22. 2005..These findings provide the first view of the architecture of a transcriptional complex that assembles at an RNA polymerase II-dependent gene promoter in a highly divergent eukaryote... - RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factorAnish Das
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, International Center for Public Health, Newark 07103, USA
Proc Natl Acad Sci U S A 100:80-5. 2003..These findings identify a surprising link in the transcriptional machinery across a large evolutionary distance in the regulation of small nuclear RNA genes in eukaryotes... - A divergent transcription factor TFIIB in trypanosomes is required for RNA polymerase II-dependent spliced leader RNA transcription and cell viabilityJennifer B Palenchar
Department of Microbiology and Molecular Genetics, UMDNJ-NJ Medical School, International Center for Public Health, 225 Warren St, Newark, NJ 07103, USA
Eukaryot Cell 5:293-300. 2006..The information gleaned from TbTFIIB studies furthers our understanding of SL RNA gene transcription and the elusive overall transcriptional processes in trypanosomes... - Genetic regulation of protein synthesis in trypanosomesAnish Das
Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, International Center for Public Health, Newark, New Jersey 07013, USA
Curr Mol Med 4:577-84. 2004..Exquisitely detailed studies of these organisms reveal basic differences in gene expression that separate these single celled eukaryotes from multicellular eukaryotic organisms and have suggested numerous potential drug targets... - Biochemical characterization of Trypanosoma brucei RNA polymerase IIAnish Das
Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, International Center for Public Health, 225 Warren Street, Newark, NJ 07103, USA
Mol Biochem Parasitol 150:201-10. 2006..brucei RNA polymerase II. While both bioinformatics and biochemical analysis have focused on the subunit structure of trypanosome RNA polymerases, this is the first study that reveals a functional RNA polymerase II enzyme... - Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: substrate specificity studies on the recombinant and endogenous proteinsHiren Banerjee
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey 07103, USA
RNA 15:1554-64. 2009..This hypermethylated dinucleotide is unique to trypanosomes and may be an intermediate in the decay of cap 4, i.e., m(7)Gpppm(3)(N6, N6, 2'O)Apm(2'O)Apm(2'O)Cpm(2)(N3, 2'O)U, that occurs in these organisms... 
Detecting conserved secondary structures in RNA molecules using constrained structural alignmentMugdha Khaladkar
Bioinformatics Program and Department of Computer Science, New Jersey Institute of Technology, Newark, NJ 07102, USA
Comput Biol Chem 32:264-72. 2008..Experimental results based on detecting internal ribosome entry sites in the RNA molecules of hepatitis C virus and Trypanosoma brucei demonstrate the effectiveness of the proposed method and its superiority over existing techniques...- Gene transcription in trypanosomesJennifer B Palenchar
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry-New Jersey Medical School, Newark, 07103, USA
Mol Biochem Parasitol 146:135-41. 2006..Pre-mRNA start sites remain elusive. In addition, two important life cycle stage-specific mRNAs are transcribed by RNA polymerase I. This review interprets these unusual transcription traits in the context of parasite biology... - Structure of the C-terminal domain of transcription factor IIB from Trypanosoma bruceiB Syed Ibrahim
Public Health Research Institute and Department of Biochemistry and Molecular Biology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 225 Warren Street, Newark, NJ 07103, USA
Proc Natl Acad Sci U S A 106:13242-7. 2009..These attributes probably mediate trypanosome-specific interactions and have implications for the apparent bidirectional transcription by RNA polymerase II in protein-encoding gene expression in these organisms... - In vivo transcription analysis utilizing chromatin immunoprecipation reveals a role for trypanosome transcription factor PBP-1 in RNA polymerase III-dependent transcriptionGwen Gilinger
Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, International Center for Public Health, Newark, NJ 07103, USA
Mol Biochem Parasitol 134:169-73. 2004 - PolyA-specific ribonuclease (PARN-1) function in stage-specific mRNA turnover in Trypanosoma bruceiChristopher J Utter
Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry New Jersey Medical School, Newark, NJ 07103, USA
Eukaryot Cell 10:1230-40. 2011..Interestingly, a subset of mRNAs was affected, including a family of mRNAs that encode stage-specific coat proteins. These data suggest that PARN-1 functions in stage-specific protein production... - RNA interference as a genetic tool in trypanosomesVivian Bellofatto
Department of Microbiology and Molecular Genetics, UMDNJ NJ Medical School, International Center for Public Health, Newark, NJ, USA
Methods Mol Biol 442:83-94. 2008..The key steps for RNAi-based reduction of gene expression, including parasite cell culture, DNA transfection, RNAi expression, and experimental execution, are discussed with a focus on procyclic forms of Trypanosoma brucei... - RNA polymerase transcription machinery in trypanosomesAnish Das
Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, 225 Warren St, Newark, NJ 07103, USA
Eukaryot Cell 7:429-34. 2008
Research Grants
- ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFERVivian Bellofatto; Fiscal Year: 1993....
- Regulation of mRNA Turnover in TrypanosomesVivian Bellofatto; Fiscal Year: 2007..We have recently developed a cell-free system that is competent for both of these enzymatic activities. This work is a key first step in our proposed experimental plan. ..
- ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFERVivian Bellofatto; Fiscal Year: 2007..brucei chromosome 2. We have recently developed a preinitiation complex formation assay, a Chromatin Immunoprecipation assay and a yeast two-hybrid system that are pertinent tools for our transcriptional dissections. ..
- ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFERVivian Bellofatto; Fiscal Year: 2009..The goal of our biomedical work is to define unique features of trypanosome biology and then to exploit these traits for the design of anti-parasitic chemotherapies. ..
- TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFERVivian Bellofatto; Fiscal Year: 2003..These analyses, which will include gene cloning and protein function studies, will help identify the specific mechanisms that trypanosomes employ to accomplish this fundamental cellular process. ..
- ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFERVivian Bellofatto; Fiscal Year: 2010..The goal of our biomedical work is to define unique features of trypanosome biology and then to exploit these traits for the design of anti-parasitic chemotherapies. ..