VIVIAN M BELLOFATTO

Summary

Affiliation: New Jersey
Country: USA

Publications

  1. ncbi request reprint Transcription and mRNA stability: parental guidance suggested
    Vivian Bellofatto
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
    Cell 147:1438-9. 2011
  2. ncbi request reprint Pyrimidine transport activities in trypanosomes
    Vivian Bellofatto
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA
    Trends Parasitol 23:187-9; discussion 190. 2007
  3. pmc The non-canonical CTD of RNAP-II is essential for productive RNA synthesis in Trypanosoma brucei
    Anish Das
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey, United States of America
    PLoS ONE 4:e6959. 2009
  4. pmc Trypanosomal TBP functions with the multisubunit transcription factor tSNAP to direct spliced-leader RNA gene expression
    Anish Das
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, Newark, 07103, USA
    Mol Cell Biol 25:7314-22. 2005
  5. pmc RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor
    Anish Das
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, International Center for Public Health, Newark 07103, USA
    Proc Natl Acad Sci U S A 100:80-5. 2003
  6. pmc A divergent transcription factor TFIIB in trypanosomes is required for RNA polymerase II-dependent spliced leader RNA transcription and cell viability
    Jennifer B Palenchar
    Department of Microbiology and Molecular Genetics, UMDNJ NJ Medical School, International Center for Public Health, 225 Warren St, Newark, NJ 07103, USA
    Eukaryot Cell 5:293-300. 2006
  7. ncbi request reprint Genetic regulation of protein synthesis in trypanosomes
    Anish Das
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, New Jersey 07013, USA
    Curr Mol Med 4:577-84. 2004
  8. ncbi request reprint Biochemical characterization of Trypanosoma brucei RNA polymerase II
    Anish Das
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, 225 Warren Street, Newark, NJ 07103, USA
    Mol Biochem Parasitol 150:201-10. 2006
  9. pmc Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: substrate specificity studies on the recombinant and endogenous proteins
    Hiren Banerjee
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey 07103, USA
    RNA 15:1554-64. 2009
  10. doi request reprint Detecting conserved secondary structures in RNA molecules using constrained structural alignment
    Mugdha Khaladkar
    Bioinformatics Program and Department of Computer Science, New Jersey Institute of Technology, Newark, NJ 07102, USA
    Comput Biol Chem 32:264-72. 2008

Research Grants

  1. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 1993
  2. Regulation of mRNA Turnover in Trypanosomes
    Vivian Bellofatto; Fiscal Year: 2007
  3. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2007
  4. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2009
  5. TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2003
  6. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2010

Collaborators

  • Anish Das
  • Jennifer B Palenchar
  • Christopher J Utter
  • B Syed Ibrahim
  • Hiren Banerjee
  • David A Wah
  • Mugdha Khaladkar
  • Gwen Gilinger
  • Stacey A Garcia
  • Joseph Milone
  • Douglas V Laurents
  • Andrzej Guranowski
  • Janusz Stepinski
  • Elzbieta Bojarska
  • Edward Darzynkiewicz
  • Maciej Lukaszewicz
  • Jacek Jemielity
  • Nalini Kanneganti
  • Stephanie Ng
  • Gabrielle E Rieckhof
  • Jason T L Wang
  • Jeffrey Wilusz
  • Vandanaben Patel
  • Wenzhe Liu
  • Peter M Palenchar
  • Hua Luo

Detail Information

Publications16

  1. ncbi request reprint Transcription and mRNA stability: parental guidance suggested
    Vivian Bellofatto
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
    Cell 147:1438-9. 2011
    ..and Trcek et al. provide evidence that these two processes are integrated. They show that transcription factors and DNA promoters can directly influence the relative stability of transcripts that they produce...
  2. ncbi request reprint Pyrimidine transport activities in trypanosomes
    Vivian Bellofatto
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA
    Trends Parasitol 23:187-9; discussion 190. 2007
    ..The work by Gudin et al. characterizes the dynamics of these transporters and points to further areas for future genetic and therapeutic experiments...
  3. pmc The non-canonical CTD of RNAP-II is essential for productive RNA synthesis in Trypanosoma brucei
    Anish Das
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey, United States of America
    PLoS ONE 4:e6959. 2009
    ..RNAP-II with PsiCTD truncations resulted in abortive initiation of transcription. These data establish that non-canonical CTDs play an important role in gene expression...
  4. pmc Trypanosomal TBP functions with the multisubunit transcription factor tSNAP to direct spliced-leader RNA gene expression
    Anish Das
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, Newark, 07103, USA
    Mol Cell Biol 25:7314-22. 2005
    ..These findings provide the first view of the architecture of a transcriptional complex that assembles at an RNA polymerase II-dependent gene promoter in a highly divergent eukaryote...
  5. pmc RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor
    Anish Das
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, International Center for Public Health, Newark 07103, USA
    Proc Natl Acad Sci U S A 100:80-5. 2003
    ..These findings identify a surprising link in the transcriptional machinery across a large evolutionary distance in the regulation of small nuclear RNA genes in eukaryotes...
  6. pmc A divergent transcription factor TFIIB in trypanosomes is required for RNA polymerase II-dependent spliced leader RNA transcription and cell viability
    Jennifer B Palenchar
    Department of Microbiology and Molecular Genetics, UMDNJ NJ Medical School, International Center for Public Health, 225 Warren St, Newark, NJ 07103, USA
    Eukaryot Cell 5:293-300. 2006
    ..The information gleaned from TbTFIIB studies furthers our understanding of SL RNA gene transcription and the elusive overall transcriptional processes in trypanosomes...
  7. ncbi request reprint Genetic regulation of protein synthesis in trypanosomes
    Anish Das
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, New Jersey 07013, USA
    Curr Mol Med 4:577-84. 2004
    ..Exquisitely detailed studies of these organisms reveal basic differences in gene expression that separate these single celled eukaryotes from multicellular eukaryotic organisms and have suggested numerous potential drug targets...
  8. ncbi request reprint Biochemical characterization of Trypanosoma brucei RNA polymerase II
    Anish Das
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, 225 Warren Street, Newark, NJ 07103, USA
    Mol Biochem Parasitol 150:201-10. 2006
    ..brucei RNA polymerase II. While both bioinformatics and biochemical analysis have focused on the subunit structure of trypanosome RNA polymerases, this is the first study that reveals a functional RNA polymerase II enzyme...
  9. pmc Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: substrate specificity studies on the recombinant and endogenous proteins
    Hiren Banerjee
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey 07103, USA
    RNA 15:1554-64. 2009
    ..This hypermethylated dinucleotide is unique to trypanosomes and may be an intermediate in the decay of cap 4, i.e., m(7)Gpppm(3)(N6, N6, 2'O)Apm(2'O)Apm(2'O)Cpm(2)(N3, 2'O)U, that occurs in these organisms...
  10. doi request reprint Detecting conserved secondary structures in RNA molecules using constrained structural alignment
    Mugdha Khaladkar
    Bioinformatics Program and Department of Computer Science, New Jersey Institute of Technology, Newark, NJ 07102, USA
    Comput Biol Chem 32:264-72. 2008
    ..Experimental results based on detecting internal ribosome entry sites in the RNA molecules of hepatitis C virus and Trypanosoma brucei demonstrate the effectiveness of the proposed method and its superiority over existing techniques...
  11. ncbi request reprint Gene transcription in trypanosomes
    Jennifer B Palenchar
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry New Jersey Medical School, Newark, 07103, USA
    Mol Biochem Parasitol 146:135-41. 2006
    ..Pre-mRNA start sites remain elusive. In addition, two important life cycle stage-specific mRNAs are transcribed by RNA polymerase I. This review interprets these unusual transcription traits in the context of parasite biology...
  12. pmc Structure of the C-terminal domain of transcription factor IIB from Trypanosoma brucei
    B Syed Ibrahim
    Public Health Research Institute and Department of Biochemistry and Molecular Biology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 225 Warren Street, Newark, NJ 07103, USA
    Proc Natl Acad Sci U S A 106:13242-7. 2009
    ..These attributes probably mediate trypanosome-specific interactions and have implications for the apparent bidirectional transcription by RNA polymerase II in protein-encoding gene expression in these organisms...
  13. ncbi request reprint In vivo transcription analysis utilizing chromatin immunoprecipation reveals a role for trypanosome transcription factor PBP-1 in RNA polymerase III-dependent transcription
    Gwen Gilinger
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, International Center for Public Health, Newark, NJ 07103, USA
    Mol Biochem Parasitol 134:169-73. 2004
  14. pmc PolyA-specific ribonuclease (PARN-1) function in stage-specific mRNA turnover in Trypanosoma brucei
    Christopher J Utter
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry New Jersey Medical School, Newark, NJ 07103, USA
    Eukaryot Cell 10:1230-40. 2011
    ..Interestingly, a subset of mRNAs was affected, including a family of mRNAs that encode stage-specific coat proteins. These data suggest that PARN-1 functions in stage-specific protein production...
  15. doi request reprint RNA interference as a genetic tool in trypanosomes
    Vivian Bellofatto
    Department of Microbiology and Molecular Genetics, UMDNJ NJ Medical School, International Center for Public Health, Newark, NJ, USA
    Methods Mol Biol 442:83-94. 2008
    ..The key steps for RNAi-based reduction of gene expression, including parasite cell culture, DNA transfection, RNAi expression, and experimental execution, are discussed with a focus on procyclic forms of Trypanosoma brucei...
  16. pmc RNA polymerase transcription machinery in trypanosomes
    Anish Das
    Department of Microbiology and Molecular Genetics, UMDNJ New Jersey Medical School, 225 Warren St, Newark, NJ 07103, USA
    Eukaryot Cell 7:429-34. 2008

Research Grants23

  1. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 1993
    ....
  2. Regulation of mRNA Turnover in Trypanosomes
    Vivian Bellofatto; Fiscal Year: 2007
    ..We have recently developed a cell-free system that is competent for both of these enzymatic activities. This work is a key first step in our proposed experimental plan. ..
  3. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2007
    ..brucei chromosome 2. We have recently developed a preinitiation complex formation assay, a Chromatin Immunoprecipation assay and a yeast two-hybrid system that are pertinent tools for our transcriptional dissections. ..
  4. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2009
    ..The goal of our biomedical work is to define unique features of trypanosome biology and then to exploit these traits for the design of anti-parasitic chemotherapies. ..
  5. TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2003
    ..These analyses, which will include gene cloning and protein function studies, will help identify the specific mechanisms that trypanosomes employ to accomplish this fundamental cellular process. ..
  6. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2010
    ..The goal of our biomedical work is to define unique features of trypanosome biology and then to exploit these traits for the design of anti-parasitic chemotherapies. ..