Genomes and Genes
Maria E Aguero-Rosenfeld
Affiliation: New York Medical College
- Seroprevalence of antibodies that react with Anaplasma phagocytophila, the agent of human granulocytic ehrlichiosis, in different populations in Westchester County, New YorkMaria E Aguero-Rosenfeld
Division of Infectious Diseases, Department of Medicine, New York Medical College and Westchester Medical Center, Valhalla, New York 10595, USA
J Clin Microbiol 40:2612-5. 2002..phagocytophila antibodies when the conventional cutoff titer of 80 is used in the IFA. This information must be considered in interpretation of test results...
- Lyme disease: laboratory issuesMaria E Aguero-Rosenfeld
Department of Pathology, Medicine, and Microbiology, New York Medical College, Valhalla, NY 10595, USA
Infect Dis Clin North Am 22:301-13, vii. 2008..Use and limitations of these methods are discussed. Current guidelines for the use of recommended serologic methods and discussion of newer methods also are provided...
- Diagnosis of lyme borreliosisMaria E Aguero-Rosenfeld
Department of Pathology, Division of Infectious Diseases, New York Medical College, Valhalla, NY, USA
Clin Microbiol Rev 18:484-509. 2005..Evaluation of these assays is currently being done, and there is evidence that certain of these antigens may be broadly cross-reactive with the B. burgdorferi sensu lato species causing LB in Europe...
- Diagnosis of human granulocytic ehrlichiosis: state of the artMaria E Aguero-Rosenfeld
Department of Pathology, New York Medical College, Westchester Medical Center, Valhalla, New York 10595, USA
Vector Borne Zoonotic Dis 2:233-9. 2002..Serology has an important role in the confirmation of the diagnosis when used in paired specimens and when high cutoff titers by indirect fluorescence antibody assay (> or = 640) are used to diagnose a recent infection...
- Laboratory aspects of tick-borne diseases: lyme, human granulocytic ehrlichiosis and babesiosisMarie E Aguero-Rosenfeld
Clinical Laboratories, Room 1J 11a, Westchester Medical Center, Valhalla, NY 10595, USA
Mt Sinai J Med 70:197-206. 2003..Co-infections with these agents exist, but they should be documented by detection of the organisms rather than by serology, since seroprevalence rates are high in endemic areas...
- Differences and similarities between culture-confirmed human granulocytic anaplasmosis and early lyme diseaseGary P Wormser
Department of Medicine, Division of Infectious Diseases, New York Medical College, Valhalla, New York, USA
J Clin Microbiol 51:954-8. 2013..389, P = 0.009) was observed for HGA patients but not for patients with Lyme disease. In conclusion, although there are overlapping features, culture-confirmed HGA is a more severe illness than early Lyme disease...
- Single-tier testing with the C6 peptide ELISA kit compared with two-tier testing for Lyme diseaseGary P Wormser
Division of Infectious Diseases, New York Medical College, Valhalla, NY 10595, USA
Diagn Microbiol Infect Dis 75:9-15. 2013..The C6 ELISA had slightly decreased specificity. Future studies should evaluate the performance of the C6 ELISA compared with 2-tier testing in routine clinical practice...
- Dissociation between inhibition and killing by levofloxacin in human granulocytic anaplasmosisGary P Wormser
Division of Infectious Diseases, Depatment of Medicine, New York Medical College, Westcheser Medical Center, Valhalla, New York, USA
Vector Borne Zoonotic Dis 6:388-94. 2006..Relapse of infection after levofloxacin therapy was reproduced in a severe combined immune-deficient (SCID) mouse infection model. Quinolone therapy should not be considered curative of HGA...
- 2-tiered antibody testing for early and late Lyme disease using only an immunoglobulin G blot with the addition of a VlsE band as the second-tier testJohn A Branda
Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
Clin Infect Dis 50:20-6. 2010..IgG VlsE antibody testing, by itself, improves early sensitivity, but may lower specificity. We studied whether elements of the 2 approaches could be combined to produce a second-tier IgG blot that performs well throughout the infection...
- Lyme disease and human granulocytic anaplasmosis coinfection: impact of case definition on coinfection rates and illness severityHarold W Horowitz
Division of Infectious Diseases, Department of Medicine, New York Medical College, Munger Pavilion, Rm 245, Valhalla, NY 10595, USA
Clin Infect Dis 56:93-9. 2013..Conflicting data exist on the frequency of coinfection and on whether Lyme-HGA coinfected patients have more symptoms than patients with Lyme disease alone...
- Borrelia burgdorferi BmpA, BmpB, and BmpD proteins are expressed in human infection and contribute to P39 immunoblot reactivity in patients with Lyme diseaseAnton V Bryksin
Department of Microbiology and Immunology, New York Medical College, Valhalla, NY 10595, USA
Clin Diagn Lab Immunol 12:935-40. 2005..This suggests that at least three of the four Bmp proteins are expressed by B. burgdorferi in infected patients and that specific antibodies to them are likely to be present in the P39 band in some patients...
- Utility of serodiagnostics designed for use in the United States for detection of Lyme borreliosis acquired in Europe and vice versaGary P Wormser
Division of Infectious Diseases, New York Medical College, Valhalla, NY, 10595, USA
Med Microbiol Immunol 203:65-71. 2014..Two-tier serologic testing with the US test kits may be unsatisfactory for detection of LB acquired in Europe. First-tier testing with an assay such as the C6 ELISA should be considered as a stand-alone diagnostic strategy in such cases...
- Impact of clinical variables on Borrelia burgdorferi-specific antibody seropositivity in acute-phase sera from patients in North America with culture-confirmed early Lyme diseaseGary P Wormser
Department of Medicine, Division of Infectious Diseases, Munger Pavilion Room 245, New York Medical College, Valhalla, NY 10595, USA
Clin Vaccine Immunol 15:1519-22. 2008..Thus, in patients with erythema migrans, certain clinical variables such as the duration and number of skin lesions had a profound impact on seropositivity rates, irrespective of the serologic assay performed...
- Lyme disease: diagnostic issues and controversiesMaria E Aguero-Rosenfeld
Department of Pathology, New York University School of Medicine, New York University Langone Medical Center and Bellevue Hospital Center, New York, NY 10016, USA
Expert Rev Mol Diagn 15:1-4. 2015..Herein, we provide a brief overview of Lyme disease testing, emphasizing current usage and limitations. We also discuss the use of nonvalidated procedures and the prospects for a reduction in such testing practices in the future. ..
- Vertical transmission of Babesia microti, United StatesJulie T Joseph
New York Medical College, Valhalla, New York 10595, USA
Emerg Infect Dis 18:1318-21. 2012..We report a case of babesiosis in an infant for whom vertical transmission was suggested by evidence of Babesia spp. antibodies in the heel-stick blood sample and confirmed by detection of Babesia spp. DNA in placenta tissue...
- Polyphyletic emergence of linezolid-resistant staphylococci in the United StatesAgnes Wong
Department of Microbiology and Immunology, New York Medical College, Valhalla, NY, USA
Antimicrob Agents Chemother 54:742-8. 2010..epidermidis isolates. These findings suggest that linezolid resistance has emerged independently in multiple clones of S. aureus and with a variety of ribosomal mutations in multiple clones of S. epidermidis...