Igor L Medintz

Summary

Affiliation: Naval Medical Center
Country: USA

Publications

  1. pmc Potential clinical applications of quantum dots
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC, USA
    Int J Nanomedicine 3:151-67. 2008
  2. ncbi request reprint General strategy for biosensor design and construction employing multifunctional surface-tethered components
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, D C 20375, USA
    Anal Chem 76:5620-9. 2004
  3. ncbi request reprint Luminescent quantum dots in immunoassays
    Ellen R Goldman
    U S Naval Research Laboratory, Center for Bio Molecular Science and Engineering, Code 6900, Washington, DC 20375, USA
    Anal Bioanal Chem 384:560-3. 2006
  4. ncbi request reprint Polyethylene glycol-based bidentate ligands to enhance quantum dot and gold nanoparticle stability in biological media
    Bing C Mei
    Division of Optical Sciences, US Naval Research Laboratory, Washington, District of Columbia 20375, USA
    Nat Protoc 4:412-23. 2009
  5. ncbi request reprint Self-assembled TNT biosensor based on modular multifunctional surface-tethered components
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington DC 20375, USA
    Anal Chem 77:365-72. 2005
  6. ncbi request reprint Quantum dot bioconjugates for imaging, labelling and sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Nat Mater 4:435-46. 2005
  7. ncbi request reprint Quantum-dot/dopamine bioconjugates function as redox coupled assemblies for in vitro and intracellular pH sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, District of Columbia 20375, USA
    Nat Mater 9:676-84. 2010
  8. pmc Interactions between redox complexes and semiconductor quantum dots coupled via a peptide bridge
    Igor L Medintz
    Center for Bio Molecular Science and Engineering and Division of Optical Sciences, U S Naval Research Laboratory, Washington, DC 20375, USA
    J Am Chem Soc 130:16745-56. 2008
  9. doi request reprint Multifunctional compact zwitterionic ligands for preparing robust biocompatible semiconductor quantum dots and gold nanoparticles
    Kimihiro Susumu
    Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 133:9480-96. 2011
  10. doi request reprint Single-molecule colocalization studies shed light on the idea of fully emitting versus dark single quantum dots
    Thomas Pons
    US Naval Research Laboratory, Division of Optical Sciences, Washington, DC 20375, USA
    Small 7:2101-8. 2011

Collaborators

Detail Information

Publications77

  1. pmc Potential clinical applications of quantum dots
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC, USA
    Int J Nanomedicine 3:151-67. 2008
    ..We also explore toxicity issues surrounding these materials and speculate about the future uses of quantum dots in a clinical setting...
  2. ncbi request reprint General strategy for biosensor design and construction employing multifunctional surface-tethered components
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, D C 20375, USA
    Anal Chem 76:5620-9. 2004
    ..A complex set of interactions apparently exists on the sensing surface that may contribute to sensor behavior and range. This approach may represent a general way to assemble a wide range of useful biosensors...
  3. ncbi request reprint Luminescent quantum dots in immunoassays
    Ellen R Goldman
    U S Naval Research Laboratory, Center for Bio Molecular Science and Engineering, Code 6900, Washington, DC 20375, USA
    Anal Bioanal Chem 384:560-3. 2006
  4. ncbi request reprint Polyethylene glycol-based bidentate ligands to enhance quantum dot and gold nanoparticle stability in biological media
    Bing C Mei
    Division of Optical Sciences, US Naval Research Laboratory, Washington, District of Columbia 20375, USA
    Nat Protoc 4:412-23. 2009
    ..These ligands provide a straightforward means of preparing QDs and AuNPs that exhibit greater resistance to environmental changes, facilitating their effective use in bioassays and live cell imaging...
  5. ncbi request reprint Self-assembled TNT biosensor based on modular multifunctional surface-tethered components
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington DC 20375, USA
    Anal Chem 77:365-72. 2005
    ..The modular design of the sensor demonstrates that it can be easily adapted to detect a variety of different analytes...
  6. ncbi request reprint Quantum dot bioconjugates for imaging, labelling and sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Nat Mater 4:435-46. 2005
    ..The potential of QDs in biology has just begun to be realized and new avenues will arise as our ability to manipulate these materials improves...
  7. ncbi request reprint Quantum-dot/dopamine bioconjugates function as redox coupled assemblies for in vitro and intracellular pH sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, District of Columbia 20375, USA
    Nat Mater 9:676-84. 2010
    ..A detailed mechanism describing the QD quenching processes that is consistent with dopamine's inherent redox chemistry is presented...
  8. pmc Interactions between redox complexes and semiconductor quantum dots coupled via a peptide bridge
    Igor L Medintz
    Center for Bio Molecular Science and Engineering and Division of Optical Sciences, U S Naval Research Laboratory, Washington, DC 20375, USA
    J Am Chem Soc 130:16745-56. 2008
    ..In addition, partial bleaching of the absorption was measured for the QD-metal complex assemblies. These proximity driven interactions were further used to construct sensing assemblies to detect proteolytic enzyme activity...
  9. doi request reprint Multifunctional compact zwitterionic ligands for preparing robust biocompatible semiconductor quantum dots and gold nanoparticles
    Kimihiro Susumu
    Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 133:9480-96. 2011
    ..The strong potential of these ligands to expand NP capabilities in many biological applications is highlighted...
  10. doi request reprint Single-molecule colocalization studies shed light on the idea of fully emitting versus dark single quantum dots
    Thomas Pons
    US Naval Research Laboratory, Division of Optical Sciences, Washington, DC 20375, USA
    Small 7:2101-8. 2011
    ....
  11. doi request reprint Cytotoxicity of quantum dots used for in vitro cellular labeling: role of QD surface ligand, delivery modality, cell type, and direct comparison to organic fluorophores
    Christopher E Bradburne
    Center for Bio Molecular Science and Engineering, Code 6900, and Optical Sciences Division, Code 5600, U S Naval Research Laboratory, Washington, DC 20375, United States
    Bioconjug Chem 24:1570-83. 2013
    ..Cumulatively, our results demonstrate that the proper tuning of QD dose, surface ligand, and delivery modality can provide robust in vitro cell labeling reagents that exhibit minimal impact on cellular viability. ..
  12. doi request reprint Achieving effective terminal exciton delivery in quantum dot antenna-sensitized multistep DNA photonic wires
    Christopher M Spillmann
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, United States
    ACS Nano 7:7101-18. 2013
    ..5). The results are discussed in the context of improving exciton transfer efficiency along photonic wires and the contributions these architectures can make to understanding multistep FRET processes...
  13. doi request reprint Spatiotemporal multicolor labeling of individual cells using peptide-functionalized quantum dots and mixed delivery techniques
    James B Delehanty
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
    J Am Chem Soc 133:10482-9. 2011
    ..Using this overall approach, QDs were targeted to both early and late endosomes, the cellular cytosol, and the plasma membrane in live cells, ultimately allowing for simultaneous five-color fluorescent imaging...
  14. doi request reprint Combining chemoselective ligation with polyhistidine-driven self-assembly for the modular display of biomolecules on quantum dots
    Duane E Prasuhn
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    ACS Nano 4:267-78. 2010
    ..This modular approach for displaying peptides or DNA on QDs may be extended to other more complex biomolecules such as proteins or utilized with different types of nanoparticle materials...
  15. doi request reprint Evaluation of diverse peptidyl motifs for cellular delivery of semiconductor quantum dots
    Kelly Boeneman Gemmill
    Center for Bio Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Anal Bioanal Chem 405:6145-54. 2013
    ..Potential applications of these peptides in the context of cellular delivery of nanoparticles and a variety of other (bio)molecules are discussed...
  16. doi request reprint Quantum dots and fluorescent protein FRET-based biosensors
    Kelly Boeneman
    Center for Biomolecular Science and Engineering, Washington, DC 20375, USA
    Adv Exp Med Biol 733:63-74. 2012
    ..We further show that QDs and fluorescent proteins can be conjugated together intracellularly with strong potential for live-cell imaging and biosensing applications...
  17. doi request reprint Quantum dot DNA bioconjugates: attachment chemistry strongly influences the resulting composite architecture
    Kelly Boeneman
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington D C 20375, USA
    ACS Nano 4:7253-66. 2010
    ..These results suggest that if a particular QD biocomposite structure is desired, for example, random versus oriented, the type of bioconjugation chemistry utilized will be a key influencing factor...
  18. doi request reprint Self-assembled quantum dot-sensitized multivalent DNA photonic wires
    Kelly Boeneman
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    J Am Chem Soc 132:18177-90. 2010
    ..Integrating such DNA-based photonic structures with QDs can help create a new generation of biophotonic wire assemblies with widespread potential in nanotechnology...
  19. doi request reprint Proteolytic activity at quantum dot-conjugates: kinetic analysis reveals enhanced enzyme activity and localized interfacial "hopping"
    W Russ Algar
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, D C 20375, United States
    Nano Lett 12:3793-802. 2012
    ..A "hopping" mode of proteolysis at the nanoparticle was identified, confirming enhanced activity...
  20. doi request reprint Multimodal characterization of a linear DNA-based nanostructure
    Susan Buckhout-White
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, D C 20375, United States
    ACS Nano 6:1026-43. 2012
    ....
  21. doi request reprint Cellular uptake and fate of PEGylated gold nanoparticles is dependent on both cell-penetration peptides and particle size
    Eunkeu Oh
    Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, DC 20375, USA
    ACS Nano 5:6434-48. 2011
    ..The 16 nm and larger AuNPs did not enter the cells and were located at the cellular periphery. A preliminary assessment of cytotoxicity demonstrated minimal effects on cellular viability following peptide-mediated uptake...
  22. ncbi request reprint A hybrid quantum dot-antibody fragment fluorescence resonance energy transfer-based TNT sensor
    Ellen R Goldman
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 127:6744-51. 2005
    ..Addition of soluble TNT displaces the dye-labeled analogue, eliminating FRET and resulting in a concentration-dependent recovery of QD photoluminescence. Sensor performance and specificity were evaluated...
  23. ncbi request reprint Delivering quantum dot-peptide bioconjugates to the cellular cytosol: escaping from the endolysosomal system
    James B Delehanty
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
    Integr Biol (Camb) 2:265-77. 2010
    ..Importantly, this QD-peptide bioconjugate elicited minimal cytotoxicity in the cell lines tested...
  24. ncbi request reprint Competition between Förster resonance energy transfer and electron transfer in stoichiometrically assembled semiconductor quantum dot-fullerene conjugates
    Michael H Stewart
    Optical Sciences Division, Code 5611, Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, United States
    ACS Nano 7:9489-505. 2013
    ..These results highlight several important factors that must be considered when designing QD-donor/C60-acceptor systems for potential optoelectronic and biosensing applications. ..
  25. ncbi request reprint Optimizing protein coordination to quantum dots with designer peptidyl linkers
    Kelly Boeneman Gemmill
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    Bioconjug Chem 24:269-81. 2013
    ..Implications for these findings on the development of QD-based biosensors are discussed...
  26. doi request reprint Intracellular delivery of quantum dot-protein cargos mediated by cell penetrating peptides
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    Bioconjug Chem 19:1785-95. 2008
    ..Use of QD-peptide/fluorescent protein vectors may make powerful tools for understanding the mechanisms of nanoparticle-mediated drug delivery...
  27. ncbi request reprint On the quenching of semiconductor quantum dot photoluminescence by proximal gold nanoparticles
    Thomas Pons
    U S Naval Research Laboratory, Division of Optical Sciences, Washington, DC 20375, USA
    Nano Lett 7:3157-64. 2007
    ....
  28. doi request reprint Intracellular bioconjugation of targeted proteins with semiconductor quantum dots
    Kelly Boeneman
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, 4555 Overlook Avenue, S W, Washington, DC 20375, USA
    J Am Chem Soc 132:5975-7. 2010
    ..This approach can facilitate long-term monitoring of their spatio-temporal activity or, alternatively, allow engineering and in situ assembly of designer chimeric QD-fluorescent protein sensors...
  29. doi request reprint Synthesizing and modifying peptides for chemoselective ligation and assembly into quantum dot-peptide bioconjugates
    W Russ Algar
    Naval Research Laboratory, Washington, DC, USA
    Methods Mol Biol 1025:47-73. 2013
    ..Overall, the bioconjugate chemistry is robust, selective, and modular, thereby potentiating the controlled conjugation of QDs with a diverse array of biomolecules for various applications. ..
  30. doi request reprint Quantum dots as simultaneous acceptors and donors in time-gated Förster resonance energy transfer relays: characterization and biosensing
    W Russ Algar
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington DC 20375, USA
    J Am Chem Soc 134:1876-91. 2012
    ....
  31. doi request reprint Semiconductor quantum dots in bioanalysis: crossing the valley of death
    W Russ Algar
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, 4555 Overlook Avenue, South West, Washington, DC 20375, USA
    Anal Chem 83:8826-37. 2011
    ..We describe the burgeoning role of QDs in many different fields of bioanalyses and highlight the advantages afforded by their unique physical and optical properties...
  32. doi request reprint Multiplex charge-transfer interactions between quantum dots and peptide-bridged ruthenium complexes
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    Anal Chem 81:4831-9. 2009
    ....
  33. ncbi request reprint A reactive peptidic linker for self-assembling hybrid quantum dot-DNA bioconjugates
    Igor L Medintz
    U S Naval Research Laboratory, Center for Bio Molecular Science and Engineering Code 6900, Division of Optical Sciences Code 5611, Washington, DC 20375, USA
    Nano Lett 7:1741-8. 2007
    ....
  34. ncbi request reprint Multidentate poly(ethylene glycol) ligands provide colloidal stability to semiconductor and metallic nanocrystals in extreme conditions
    Michael H Stewart
    Optical Sciences Division, Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 132:9804-13. 2010
    ..The improved colloidal stability of nanocrystals afforded by the tetradentate ligands was further demonstrated via the assembly of stable QD-nuclear localization signal peptide bioconjugates that promoted intracellular uptake...
  35. ncbi request reprint Solution-phase single quantum dot fluorescence resonance energy transfer
    Thomas Pons
    Optical Sciences Division, Code 5611, Center for Bio Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 128:15324-31. 2006
    ..The binding constant derived from spFRET is consistent with ensemble measurements...
  36. doi request reprint Site-specific cellular delivery of quantum dots with chemoselectively-assembled modular peptides
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, USA
    Chem Commun (Camb) 49:7878-80. 2013
    ..Peptides were ratiometrically assembled onto quantum dots to facilitate their specific delivery to either the plasma membrane, endosomes, the cytosol or the mitochondria of target cells. ..
  37. doi request reprint Assembly of a concentric Förster resonance energy transfer relay on a quantum dot scaffold: characterization and application to multiplexed protease sensing
    W Russ Algar
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    ACS Nano 6:11044-58. 2012
    ..The concentric FRET relay is thus a potentially powerful tool for the characterization of QD bioconjugates and multiplexed sensing of coupled biological activity...
  38. doi request reprint Active cellular sensing with quantum dots: transitioning from research tool to reality; a review
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    Anal Chim Acta 750:63-81. 2012
    ..A perspective on where this field is expected to develop in both the near and long-term is also provided...
  39. doi request reprint Polyvalent display and packing of peptides and proteins on semiconductor quantum dots: predicted versus experimental results
    Duane E Prasuhn
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    Small 6:555-64. 2010
    ..These results can contribute towards an overall understanding of how to engineer designer bioconjugates for both QDs and other nanoparticle materials...
  40. doi request reprint Multivalent conjugation of peptides, proteins, and DNA to semiconductor quantum dots
    Duane E Prasuhn
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC, USA
    Methods Mol Biol 726:95-110. 2011
    ..These approaches can be successfully employed in the construction of a variety of QD-biomolecule constructs utilizing synthetic peptides, recombinant proteins, peptides, and even modified DNA oligomers...
  41. ncbi request reprint Multiplexed toxin analysis using four colors of quantum dot fluororeagents
    Ellen R Goldman
    Center for Bio Molecular Science and Engineering and Division of Optical Sciences, U S Naval Research Laboratory, Washington, D C 20375, USA
    Anal Chem 76:684-8. 2004
    ..Using a simple linear equation-based algorithm, it was possible to deconvolute the signal from mixed toxin samples, which allowed quantitation of all four toxins simultaneously...
  42. ncbi request reprint Enhancing the stability and biological functionalities of quantum dots via compact multifunctional ligands
    Kimihiro Susumu
    Division of Optical Sciences and Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 129:13987-96. 2007
    ..The new functional surface ligands described here provide not only stable and highly water-soluble QDs but also simple and easy access to various biological entities...
  43. ncbi request reprint Quantum dot-based multiplexed fluorescence resonance energy transfer
    Aaron R Clapp
    U S Naval Research Laboratory, Optical Sciences Division, Code 5611, Washington, DC 20375, USA
    J Am Chem Soc 127:18212-21. 2005
    ..Steady-state fluorescence results were corroborated by time-resolved measurements where selective shortening of QD lifetime was measured only for populations that were selectively engaged in nonradiative energy transfer...
  44. doi request reprint Single domain antibody-quantum dot conjugates for ricin detection by both fluoroimmunoassay and surface plasmon resonance
    George P Anderson
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
    Anal Chim Acta 786:132-8. 2013
    ....
  45. pmc Selecting improved peptidyl motifs for cytosolic delivery of disparate protein and nanoparticle materials
    Kelly Boeneman
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, United States
    ACS Nano 7:3778-96. 2013
    ....
  46. doi request reprint Complex Förster energy transfer interactions between semiconductor quantum dots and a redox-active osmium assembly
    Michael H Stewart
    Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, DC 20375, USA
    ACS Nano 6:5330-47. 2012
    ....
  47. ncbi request reprint Synthesis of compact multidentate ligands to prepare stable hydrophilic quantum dot fluorophores
    H Tetsuo Uyeda
    US Naval Research Laboratory, Division of Optical Sciences, Center for Bio Molecular Science and Engineering, Washington, DC 20375, USA
    J Am Chem Soc 127:3870-8. 2005
    ..These ligands provide a straightforward means of preparing QDs that exhibit greater resistance to environmental changes, making them more amenable for use in live cell imaging and other biotechnological applications...
  48. doi request reprint Quantum dot peptide biosensors for monitoring caspase 3 proteolysis and calcium ions
    Duane E Prasuhn
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, 4555 Overlook Ave, S W, Washington, D C 20375, USA
    ACS Nano 4:5487-97. 2010
    ..These results highlight the potential for combining peptides with QDs using different chemistries to create sensors for monitoring chemical compounds and biological processes...
  49. ncbi request reprint Can luminescent quantum dots be efficient energy acceptors with organic dye donors?
    Aaron R Clapp
    US Naval Research Laboratory, Optical Sciences Division, Code 5611, Washington, DC 20375, USA
    J Am Chem Soc 127:1242-50. 2005
    ..This is due to the long exciton lifetime of the acceptor compared to that of the dye, combined with substantial QD direct excitation...
  50. doi request reprint Multiplexed tracking of protease activity using a single color of quantum dot vector and a time-gated Förster resonance energy transfer relay
    W Russ Algar
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, United States
    Anal Chem 84:10136-46. 2012
    ..Prospective applications in biochemical research, applied diagnostics, and drug discovery are discussed...
  51. ncbi request reprint Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors
    Aaron R Clapp
    Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 126:301-10. 2004
    ....
  52. ncbi request reprint Hydrodynamic dimensions, electrophoretic mobility, and stability of hydrophilic quantum dots
    Thomas Pons
    Optical Sciences Division and Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA
    J Phys Chem B 110:20308-16. 2006
    ..These properties are critical for the design of QD-based biosensing assays as well as QD bioconjugate diffusion in live cells...
  53. ncbi request reprint Maltose-binding protein: a versatile platform for prototyping biosensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, Laboratory for the Structure of Matter, Code 6812, US Naval Research Laboratory, WA 20375 5320, USA
    Curr Opin Biotechnol 17:17-27. 2006
    ....
  54. ncbi request reprint Peptides for specific intracellular delivery and targeting of nanoparticles: implications for developing nanoparticle-mediated drug delivery
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Ther Deliv 1:411-33. 2010
    ..The paper concludes with a brief forward-looking perspective discussing what can be expected as this field develops in the coming years...
  55. ncbi request reprint Application of a homogenous assay for the detection of 2,4,6-trinitrotoluene to environmental water samples
    Ellen R Goldman
    U S Naval Research Laboratory, Center for Bio Molecular Science and Engineering, Washington, DC 20375, USA
    ScientificWorldJournal 5:445-51. 2005
    ..17 M) significantly improved the range over which the assay functioned in several river water samples...
  56. ncbi request reprint Fluoroimmunoassays using antibody-conjugated quantum dots
    Ellen R Goldman
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, USA
    Methods Mol Biol 303:19-34. 2005
    ....
  57. ncbi request reprint Surface-immobilized self-assembled protein-based quantum dot nanoassemblies
    Kim E Sapsford
    Center for Bio Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, D C 20375, USA
    Langmuir 20:7720-8. 2004
    ..The utility of this self-assembly strategy is further demonstrated by assembling a QD-protein structure that allows the QDs to engage in FRET with a dye located on the surface-covering protein...
  58. ncbi request reprint Proteolytic activity monitored by fluorescence resonance energy transfer through quantum-dot-peptide conjugates
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Nat Mater 5:581-9. 2006
    ..We also screened a number of inhibitory compounds against the QD-thrombin conjugate. This technology is not limited to sensing proteases, but may be amenable to monitoring other enzymatic modifications...
  59. doi request reprint The controlled display of biomolecules on nanoparticles: a challenge suited to bioorthogonal chemistry
    W Russ Algar
    Center for Bio Molecular Science and Engineering, Optical Sciences Division, U S Naval Research Laboratory, 4555 Overlook Avenue S W, Washington, DC 20375, United States
    Bioconjug Chem 22:825-58. 2011
    ..Potential chemistries that have not yet been applied to NPs are also discussed, and an outlook on future developments in this field is given...
  60. ncbi request reprint Reversible modulation of quantum dot photoluminescence using a protein- bound photochromic fluorescence resonance energy transfer acceptor
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, Division of Optical Sciences, Code 5611, U S Naval Research Laboratory, Washington, DC 20375, USA
    J Am Chem Soc 126:30-1. 2004
    ..This result suggests a possible use of BIPS-labeled proteins in QD-based nanostructures as part of a threshold switch or other biosensing device...
  61. ncbi request reprint Self-assembled nanoscale biosensors based on quantum dot FRET donors
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6910, US Naval Research Laboratory, Washington, DC 20375, USA
    Nat Mater 2:630-8. 2003
    ..Quantum dot-biomolecule assemblies constructed using these methods may facilitate development of new hybrid sensing materials...
  62. doi request reprint Quantum dots: a powerful tool for understanding the intricacies of nanoparticle-mediated drug delivery
    James B Delehanty
    Center for Bio Molecular Science and Engineering, SW Washington, DC 20375, USA
    Expert Opin Drug Deliv 6:1091-112. 2009
    ..Important related issues such as QD biofunctionalization and toxicity are also discussed. The paper concludes with a perspective of how this field can be expected to develop in the future...
  63. ncbi request reprint Recent progress in developing FRET-based intracellular sensors for the detection of small molecule nutrients and ligands
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900 U S Naval Research Laboratory, 4555 Overlook Ave, SW Washington, DC 20375, USA
    Trends Biotechnol 24:539-42. 2006
    ..The insight gained from designing these sensors, along with the preliminary results gathered from their first application, serve to illustrate the impact that they can have on improving our fundamental understanding of biology...
  64. pmc Sensing caspase 3 activity with quantum dot-fluorescent protein assemblies
    Kelly Boeneman
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
    J Am Chem Soc 131:3828-9. 2009
    ....
  65. ncbi request reprint TNT detection using multiplexed liquid array displacement immunoassays
    George P Anderson
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA
    Anal Chem 78:2279-85. 2006
    ..Seawater required dilution with two parts buffer to avoid loss of microspheres, while the acetone extracts were diluted 100-fold or more to minimize solvent affects...
  66. pmc Self-assembled quantum dot-peptide bioconjugates for selective intracellular delivery
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, D C 20375, USA
    Bioconjug Chem 17:920-7. 2006
    ..The efficacy of using peptides for selective intracellular delivery is highlighted by performing a multicolor QD labeling, where we found that the presence or absence of peptide on the QD surface controls cellular uptake...
  67. doi request reprint Delivering quantum dots into cells: strategies, progress and remaining issues
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC, USA
    Anal Bioanal Chem 393:1091-105. 2009
    ..Finally, we conclude by providing a perspective of how this field can be expected to develop in the future...
  68. ncbi request reprint A fluorescence resonance energy transfer sensor based on maltose binding protein
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington DC 20375, USA
    Bioconjug Chem 14:909-18. 2003
    ..In the use of these FRET pairs, MBP dissociation values for maltose were estimated (0.14-2.90 microM). Maltose limits of detection were in the 50-100 nm range...
  69. ncbi request reprint Förster resonance energy transfer investigations using quantum-dot fluorophores
    Aaron R Clapp
    US Naval Research Laboratory, Optical Sciences Division, Code 5611, Washington, DC 20375, USA
    Chemphyschem 7:47-57. 2006
    ..We also review the recent developments made in using QD bioreceptor conjugates to design FRET-based assays...
  70. doi request reprint Quantum dot-based resonance energy transfer and its growing application in biology
    Igor L Medintz
    US Naval Research Laboratory, Center for Bio Molecular Science and Engineering, Code 6910, and Division of Optical Sciences, Code 5611, 4555 Overlook Ave, S W Washington DC, 20375, USA
    Phys Chem Chem Phys 11:17-45. 2009
    ..We conclude by providing an assessment of where QD-based FRET investigations may be evolving in the near future...
  71. ncbi request reprint Materials for fluorescence resonance energy transfer analysis: beyond traditional donor-acceptor combinations
    Kim E Sapsford
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Code 6910, 4555 Overlook Avenue SW, Washington, DC 20375, USA
    Angew Chem Int Ed Engl 45:4562-89. 2006
    ..We focus in particular on the benefits and limitations of these materials and their combinations, as well as the available methods of bioconjugation...
  72. ncbi request reprint Designer variable repeat length polypeptides as scaffolds for surface immobilization of quantum dots
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    J Phys Chem B 110:10683-90. 2006
    ..The versatility of this capture strategy is highlighted by the creation of a variety of one- and two-dimensional polypeptide-QD structures as well as a self-assembled surface-immobilized FRET-based nutrient sensor...
  73. ncbi request reprint Decoration of discretely immobilized cowpea mosaic virus with luminescent quantum dots
    Igor L Medintz
    Center for Bio Molecular Science and Engineering Code 6900, Laboratory for the Structure of Matter Code 6812, Washington, DC 20375, USA
    Langmuir 21:5501-10. 2005
    ..Characterization of the assembled CPMV and QD structures is presented, and the potential uses for protein-coated QDs functionalized onto this symmetrical virion nanoscaffold are discussed...
  74. ncbi request reprint Transcript and proteomic analyses of wild-type and gpa2 mutant Saccharomyces cerevisiae strains suggest a role for glycolytic carbon source sensing in pseudohyphal differentiation
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Mol Biosyst 3:623-34. 2007
    ....
  75. doi request reprint Elaborate nanoparticle-based traps for catching cytosolic players in the act
    James B Delehanty
    Center for Bio Molecular Science and Engineering, US Naval Research Laboratory, Code 6900, Washington DC 20375 USA
    Chembiochem 13:30-3. 2012
    ..The system uses the rapid, innate self-assembly of ferritin, expressing bait and prey molecules, into ordered nanoclusters whose morphology is tracked by using fluorescence microscopy...
  76. ncbi request reprint Novel energy transfer fluorescence labeling cassette
    Igor L Medintz
    Biotechniques 32:270, 272. 2002
  77. ncbi request reprint Microfabricated 384-lane capillary array electrophoresis bioanalyzer for ultrahigh-throughput genetic analysis
    Charles A Emrich
    Department of Chemistry, University of California, Berkeley 94720, USA
    Anal Chem 74:5076-83. 2002
    ..This lab-on-a-chip device thoroughly exploits the power of microfabrication to produce high-density capillary electrophoresis arrays and to use them for high-throughput bioanalysis...