Research Topics
Species | C G HayesSummaryAffiliation: Naval Medical Center Country: USA Publications
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Detail Information
Publications
West Nile virus: Uganda, 1937, to New York City, 1999C G Hayes
Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, Maryland 20910 7500, USA
Ann N Y Acad Sci 951:25-37. 2001..This review will attempt to compare observations on the biology of West Nile virus made over about 60 years prior to the recent epidemics to observations made in association with these urban epidemics...- Characterization of antibody responses to combinations of a dengue-2 DNA and dengue-2 recombinant subunit vaccineM Simmons
Virology Program, Naval Medical Research Center, Silver Spring, Maryland 20910-7500, USA
Am J Trop Med Hyg 65:420-6. 2001..Our observations indicate that the combination RD prime-boost regimen warrants further investigation as a vaccine strategy to prevent dengue infection... 
Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virusJ D Callahan
Viral and Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland 20910-7500, USA
J Clin Microbiol 39:4119-24. 2001..Our results demonstrate that the dengue virus TaqMan RT-PCR assays may be utilized as rapid, sensitive, and specific screening and serotyping tools for epidemiological studies of dengue virus infections...- Hepatitis E virus DNA vaccine elicits immunologic memory in miceJ He
Department of Virus Diseases, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Silver Spring, MD 20910, USA
J Biomed Sci 8:223-6. 2001..We speculate that this type of response could protect a human from overt disease... - A dengue virus serotype-1 DNA vaccine induces virus neutralizing antibodies and provides protection from viral challenge in Aotus monkeysT J Kochel
Virology Program, Naval Medical Research Center, 8901 Wisconsin Avenue, 20889 5607, Bethesda, MD, USA
Vaccine 18:3166-73. 2000..These results demonstrate that DNA immunization is a promising approach for the development of dengue vaccines and that A. nancymae monkeys are suitable for dengue vaccine trials... - Evaluation of a dipstick enzyme-linked immunosorbent assay for detection of antibodies to dengue virusS J Wu
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889 5607, USA
Clin Diagn Lab Immunol 4:452-7. 1997..The dipstick ELISA was also shown to be useful for detecting seroconversions to DEN IgM or IgG in paired serum samples from 20 patients with virus isolation-confirmed acute DEN infections... - Short report: Antibody responses of mice immunized with a tetravalent dengue recombinant protein subunit vaccineM Simmons
Virology Program, Naval Medical Research Center, Silver Spring, Maryland 20910 7500, USA
Am J Trop Med Hyg 65:159-61. 2001.... - Detection of dengue viral RNA using a nucleic acid sequence-based amplification assayS J Wu
Viral Diseases Department, Naval Medical Research Center, Silver Spring, Maryland 20910 7500, USA
J Clin Microbiol 39:2794-8. 2001..The NASBA assay had a specificity of 100% based on the negative test results for the 21 normal human serum or plasma samples. These results indicate that the NASBA assay is a promising assay for the early diagnosis of dengue infections... - Synergistic neutralizing antibody response to a dengue virus type 2 DNA vaccine by incorporation of lysosome-associated membrane protein sequences and use of plasmid expressing GM-CSFK Raviprakash
Virology Program, Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
Virology 290:74-82. 2001..The highest antibody responses were noted when the modified construct was coinjected with plasmid expressing the GM-CSF gene. These results could form the basis for an effective tetravalent dengue virus DNA vaccine... - Dengue virus type 1 DNA vaccine induces protective immune responses in rhesus macaquesK Raviprakash
Virology Program, Infectious Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Rm 3N71, Silver Spring, MD 20910 7500, USA
J Gen Virol 81:1659-67. 2000..Vaccine-induced antibody had an avidity index similar to that of antibody induced by virus infection; however, no clear correlation was apparent between antibody avidity and virus neutralization titres... - Construction of a full length infectious clone for dengue-1 virus Western Pacific,74 strainB Puri
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, MD 20889 5607, USA
Virus Genes 20:57-63. 2000..Biological properties of the transcript-derived virus, such as the pattern of dengue-specific protein synthesis and growth rate in LLCMK2 or C6/36 cells, resembled those of the parent DEN1 WP virus... - Immunogenicity of dengue virus type 1 DNA vaccines expressing truncated and full length envelope proteinK Raviprakash
Virology Program, Infectious Diseases Department, Naval Medical Research Institute, 8901 Wisconsin Avenue, Bethesda, MD, USA
Vaccine 18:2426-34. 2000..Data suggested that the plasmid expressing prM and full length E produced virus like particles in transfected cells, and is probably a better immunogen compared to that expressing 80% E... - Comparison of two rapid diagnostic assays for detection of immunoglobulin M antibodies to dengue virusS J Wu
Viral and Rickettsial Diseases Department, Naval Medical Research Center, Bethesda, Maryland 20889 5607, USA
Clin Diagn Lab Immunol 7:106-10. 2000..3 and 97.1%, respectively. Both commercial assays provide sensitive and specific detection of anti-dengue virus IgM antibody and could prove useful in settings where the microplate ELISA is impractical... - Complete nucleotide sequence analysis of a Western Pacific dengue-1 virus strainB Puri
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889 5607, USA
Virus Genes 17:85-8. 1998..When compared to DEN-1 Singapore S275/90, the nucleotide and amino acid sequence homology are 94% and 97.8%, respectively... - Molecular analysis of dengue virus attenuation after serial passage in primary dog kidney cellsB Puri
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889 5607, USA
J Gen Virol 78:2287-91. 1997..Important nucleotide base changes responsible for attenuation accumulated between 45AZ5 PDK-O and 27... - Molecular characterization of a hepatitis E virus isolate from NamibiaJ He
Department of Virus Diseases, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Silver Spring, MD 20910, USA
J Biomed Sci 7:334-8. 2000..These data link the HEV causing the 1983 Namibia outbreak to more recent HEV transmission in northern and sub-Saharan Africa, suggesting this subgenotype of HEV is firmly established throughout the continent... - Haiti: absence of dengue hemorrhagic fever despite hyperendemic dengue virus transmissionS B Halstead
Medical Science and Technology Division, Office of Naval Research, Arlington, Virginia 22217-5660, USA
Am J Trop Med Hyg 65:180-3. 2001..These observations, which are reminiscent of those in Africa, provide further evidence of a dengue resistance gene in black populations... - Conversion of dengue virus replicative form RNA (RF) to replicative intermediate (RI) by nonstructural proteins NS-5 and NS-3K Raviprakash
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889, USA
Am J Trop Med Hyg 58:90-5. 1998..The recombinant proteins, however, required one or more cellular protein(s) for their activity. Examination of NS-3 protein sequence revealed heretofore unnoticed sequence similarities with other polymerases...