Research Topics
Species | D L DoolanSummaryAffiliation: Naval Medical Center Country: USA Publications
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Detail Information
Publications
The complexity of protective immunity against liver-stage malariaD L Doolan
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
J Immunol 165:1453-62. 2000....
HLA-DR-promiscuous T cell epitopes from Plasmodium falciparum pre-erythrocytic-stage antigens restricted by multiple HLA class II allelesD L Doolan
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
J Immunol 165:1123-37. 2000..falciparum validates our predictive strategy in a biologically relevant system and supports the potential for developing a broadly efficacious epitope-based vaccine against malaria focused on a limited number of peptide specificities...
Strategy for development of a pre-erythrocytic Plasmodium falciparum DNA vaccine for human useS L Hoffman
Malaria Program, Naval Medical Research Institute, Bethesda, MD 20889 5607, USA
Vaccine 15:842-5. 1997..We anticipate that optimization of multi-gene P. falciparum DNA vaccines designed to protect against malaria by inducing CD8+ T cells that target infected hepatocytes will require extensive clinical trials during the coming years...
Toward clinical trials of DNA vaccines against malariaS L Hoffman
Malaria Program, Naval Medical Research Institute, Bethesda, Maryland 20852, USA
Immunol Cell Biol 75:376-81. 1997..Herein, we review the rationale and experimental foundation for the anticipated P. falciparum DNA vaccine trials...
Identification of minimal CD8+ and CD4+ T cell epitopes in the Plasmodium yoelii hepatocyte erythrocyte protein 17kDaC Dobano
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
Mol Immunol 44:3037-48. 2007..The identification of these epitopes will allow the evaluation of delivery systems for malaria vaccine candidates as well as the delineation of protective immune mechanisms...
CD4(+) T-cell- and gamma interferon-dependent protection against murine malaria by immunization with linear synthetic peptides from a Plasmodium yoelii 17-kilodalton hepatocyte erythrocyte proteinY Charoenvit
Malaria Program, Naval Medical Research Center, Bethesda, Maryland 20814 5055, USA
Infect Immun 67:5604-14. 1999....
Determining liver stage parasite burden by real time quantitative PCR as a method for evaluating pre-erythrocytic malaria vaccine efficacyA A Witney
Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Room 3A4O, Silver Spring, MD 20910-7500, USA
Mol Biochem Parasitol 118:233-45. 2001..Our data establish this quantitative real-time PCR assay to be a fast and reproducible way of accurately assessing liver stage parasite burden and vaccine efficacy in rodent malaria models...
IL-12 and NK cells are required for antigen-specific adaptive immunity against malaria initiated by CD8+ T cells in the Plasmodium yoelii modelD L Doolan
Malaria Program, Naval Medical Research Center, Bethesda, MD 20889, USA
J Immunol 163:884-92. 1999....
Induction of CD4(+) T cell-dependent CD8(+) type 1 responses in humans by a malaria DNA vaccineR Wang
Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
Proc Natl Acad Sci U S A 98:10817-22. 2001..Demonstration in humans of elicitation of the class I restricted IFN-gamma responses we believe necessary for protection against the liver stage of malaria parasites brings us closer to an effective malaria vaccine...
Interleukin-12- and gamma interferon-dependent protection against malaria conferred by CpG oligodeoxynucleotide in miceR A Gramzinski
Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910-7500, USA
Infect Immun 69:1643-9. 2001..This is the first report that stimulation of the innate immune system by CpG immunostimulatory motifs can confer sterile protection against malaria...
Immune response to pre-erythrocytic stages of malaria parasitesD L Doolan
Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910 7500, USA
Curr Mol Med 6:169-85. 2006..Herein, we review our current understanding of the molecular and cellular aspects of pre-erythrocytic stage immunity...
Mutating the anchor residues associated with MHC binding inhibits and deviates CD8+ T cell mediated protective immunity against malariaC Dobano
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
Mol Immunol 44:2235-48. 2007....
DNA-based vaccines against malaria: status and promise of the Multi-Stage Malaria DNA Vaccine OperationD L Doolan
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
Int J Parasitol 31:753-62. 2001....
Expression of the chemokine MIG is a sensitive and predictive marker for antigen-specific, genetically restricted IFN-gamma production and IFN-gamma-secreting cellsG T Brice
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910-7500, USA
J Immunol Methods 257:55-69. 2001..Our data validate this novel method for the detection of low as well as high levels of antigen-specific and genetically restricted IFN-gamma activity...
Utilization of genomic sequence information to develop malaria vaccinesD L Doolan
Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
J Exp Biol 206:3789-802. 2003..Here, we present our strategy to exploit the genomic sequence of P. falciparum for malaria vaccine development...
