D L Doolan

Summary

Affiliation: Naval Medical Center
Country: USA

Publications

  1. ncbi request reprint Protection of humans against malaria by immunization with radiation-attenuated Plasmodium falciparum sporozoites
    Stephen L Hoffman
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20850, USA
    J Infect Dis 185:1155-64. 2002
  2. ncbi request reprint Nucleic acid vaccines against malaria
    Denise L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
    Chem Immunol 80:308-21. 2002
  3. ncbi request reprint Immune response to pre-erythrocytic stages of malaria parasites
    D L Doolan
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910 7500, USA
    Curr Mol Med 6:169-85. 2006
  4. ncbi request reprint The US capitol bioterrorism anthrax exposures: clinical epidemiological and immunological characteristics
    Denise L Doolan
    Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    J Infect Dis 195:174-84. 2007
  5. pmc Transcriptionally active PCR for antigen identification and vaccine development: in vitro genome-wide screening and in vivo immunogenicity
    David P Regis
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, United States
    Mol Biochem Parasitol 158:32-45. 2008
  6. ncbi request reprint Utilization of genomic sequence information to develop malaria vaccines
    D L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    J Exp Biol 206:3789-802. 2003
  7. ncbi request reprint DNA-based vaccines against malaria: status and promise of the Multi-Stage Malaria DNA Vaccine Operation
    D L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Int J Parasitol 31:753-62. 2001
  8. ncbi request reprint Strategy for development of a pre-erythrocytic Plasmodium falciparum DNA vaccine for human use
    S L Hoffman
    Malaria Program, Naval Medical Research Institute, Bethesda, MD 20889 5607, USA
    Vaccine 15:842-5. 1997
  9. ncbi request reprint Toward clinical trials of DNA vaccines against malaria
    S L Hoffman
    Malaria Program, Naval Medical Research Institute, Bethesda, Maryland 20852, USA
    Immunol Cell Biol 75:376-81. 1997
  10. ncbi request reprint The complexity of protective immunity against liver-stage malaria
    D L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
    J Immunol 165:1453-62. 2000

Detail Information

Publications36

  1. ncbi request reprint Protection of humans against malaria by immunization with radiation-attenuated Plasmodium falciparum sporozoites
    Stephen L Hoffman
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20850, USA
    J Infect Dis 185:1155-64. 2002
    ....
  2. ncbi request reprint Nucleic acid vaccines against malaria
    Denise L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
    Chem Immunol 80:308-21. 2002
  3. ncbi request reprint Immune response to pre-erythrocytic stages of malaria parasites
    D L Doolan
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910 7500, USA
    Curr Mol Med 6:169-85. 2006
    ..Herein, we review our current understanding of the molecular and cellular aspects of pre-erythrocytic stage immunity...
  4. ncbi request reprint The US capitol bioterrorism anthrax exposures: clinical epidemiological and immunological characteristics
    Denise L Doolan
    Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    J Infect Dis 195:174-84. 2007
    ..Bioterrorism-related anthrax exposures occurred at the US Capitol in 2001. Exposed individuals received antibiotics and anthrax vaccine adsorbed immunization...
  5. pmc Transcriptionally active PCR for antigen identification and vaccine development: in vitro genome-wide screening and in vivo immunogenicity
    David P Regis
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, United States
    Mol Biochem Parasitol 158:32-45. 2008
    ..These data support the potential of a TAP approach for rapid high throughput functional screening and identification of potential candidate vaccine antigens from genomic sequence data...
  6. ncbi request reprint Utilization of genomic sequence information to develop malaria vaccines
    D L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    J Exp Biol 206:3789-802. 2003
    ..Here, we present our strategy to exploit the genomic sequence of P. falciparum for malaria vaccine development...
  7. ncbi request reprint DNA-based vaccines against malaria: status and promise of the Multi-Stage Malaria DNA Vaccine Operation
    D L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Int J Parasitol 31:753-62. 2001
    ....
  8. ncbi request reprint Strategy for development of a pre-erythrocytic Plasmodium falciparum DNA vaccine for human use
    S L Hoffman
    Malaria Program, Naval Medical Research Institute, Bethesda, MD 20889 5607, USA
    Vaccine 15:842-5. 1997
    ..We anticipate that optimization of multi-gene P. falciparum DNA vaccines designed to protect against malaria by inducing CD8+ T cells that target infected hepatocytes will require extensive clinical trials during the coming years...
  9. ncbi request reprint Toward clinical trials of DNA vaccines against malaria
    S L Hoffman
    Malaria Program, Naval Medical Research Institute, Bethesda, Maryland 20852, USA
    Immunol Cell Biol 75:376-81. 1997
    ..Herein, we review the rationale and experimental foundation for the anticipated P. falciparum DNA vaccine trials...
  10. ncbi request reprint The complexity of protective immunity against liver-stage malaria
    D L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
    J Immunol 165:1453-62. 2000
    ....
  11. pmc CD4(+) T-cell- and gamma interferon-dependent protection against murine malaria by immunization with linear synthetic peptides from a Plasmodium yoelii 17-kilodalton hepatocyte erythrocyte protein
    Y Charoenvit
    Malaria Program, Naval Medical Research Center, Bethesda, Maryland 20814 5055, USA
    Infect Immun 67:5604-14. 1999
    ....
  12. ncbi request reprint Identification of minimal CD8+ and CD4+ T cell epitopes in the Plasmodium yoelii hepatocyte erythrocyte protein 17kDa
    C Dobano
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Mol Immunol 44:3037-48. 2007
    ..The identification of these epitopes will allow the evaluation of delivery systems for malaria vaccine candidates as well as the delineation of protective immune mechanisms...
  13. ncbi request reprint IL-12 and NK cells are required for antigen-specific adaptive immunity against malaria initiated by CD8+ T cells in the Plasmodium yoelii model
    D L Doolan
    Malaria Program, Naval Medical Research Center, Bethesda, MD 20889, USA
    J Immunol 163:884-92. 1999
    ....
  14. ncbi request reprint Determining liver stage parasite burden by real time quantitative PCR as a method for evaluating pre-erythrocytic malaria vaccine efficacy
    A A Witney
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Room 3A4O, Silver Spring, MD 20910-7500, USA
    Mol Biochem Parasitol 118:233-45. 2001
    ..Our data establish this quantitative real-time PCR assay to be a fast and reproducible way of accurately assessing liver stage parasite burden and vaccine efficacy in rodent malaria models...
  15. ncbi request reprint Mutating the anchor residues associated with MHC binding inhibits and deviates CD8+ T cell mediated protective immunity against malaria
    C Dobano
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Mol Immunol 44:2235-48. 2007
    ....
  16. pmc Interleukin-12- and gamma interferon-dependent protection against malaria conferred by CpG oligodeoxynucleotide in mice
    R A Gramzinski
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910-7500, USA
    Infect Immun 69:1643-9. 2001
    ..This is the first report that stimulation of the innate immune system by CpG immunostimulatory motifs can confer sterile protection against malaria...
  17. ncbi request reprint HLA-DR-promiscuous T cell epitopes from Plasmodium falciparum pre-erythrocytic-stage antigens restricted by multiple HLA class II alleles
    D L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
    J Immunol 165:1123-37. 2000
    ..falciparum validates our predictive strategy in a biologically relevant system and supports the potential for developing a broadly efficacious epitope-based vaccine against malaria focused on a limited number of peptide specificities...
  18. pmc Induction of CD4(+) T cell-dependent CD8(+) type 1 responses in humans by a malaria DNA vaccine
    R Wang
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Proc Natl Acad Sci U S A 98:10817-22. 2001
    ..Demonstration in humans of elicitation of the class I restricted IFN-gamma responses we believe necessary for protection against the liver stage of malaria parasites brings us closer to an effective malaria vaccine...
  19. ncbi request reprint Expression of the chemokine MIG is a sensitive and predictive marker for antigen-specific, genetically restricted IFN-gamma production and IFN-gamma-secreting cells
    G T Brice
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910-7500, USA
    J Immunol Methods 257:55-69. 2001
    ..Our data validate this novel method for the detection of low as well as high levels of antigen-specific and genetically restricted IFN-gamma activity...
  20. ncbi request reprint Targeting antigen to MHC Class I and Class II antigen presentation pathways for malaria DNA vaccines
    Carlota Dobano
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, United States
    Immunol Lett 111:92-102. 2007
    ..Our data highlight the antigen dependence of immune enhancement strategies that target antigen to the MHC Class I and II pathways for vaccine development...
  21. ncbi request reprint Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation
    Carlota Dobano
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, United States
    Vaccine 25:6635-45. 2007
    ..Moreover, IFN-gamma responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents...
  22. pmc Induction of multi-antigen multi-stage immune responses against Plasmodium falciparum in rhesus monkeys, in the absence of antigen interference, with heterologous DNA prime/poxvirus boost immunization
    George Jiang
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Malar J 6:135. 2007
    ....
  23. ncbi request reprint Viral vectors for malaria vaccine development
    Shengqiang Li
    PATH Malaria Vaccine Initiative, Bethesda, MD, USA
    Vaccine 25:2567-74. 2007
    ..Understanding the vector's ability to induce an immune response and the expression of malaria antigens in mammalian cells will be critical in designing the next generation of viral-vectored malaria vaccines...
  24. ncbi request reprint Extended immunization intervals enhance the immunogenicity and protective efficacy of plasmid DNA vaccines
    Gary T Brice
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Microbes Infect 9:1439-46. 2007
    ..Our studies establish that the dosing interval significantly impacts the immunogenicity and protective efficacy of plasmid DNA vaccines...
  25. ncbi request reprint Vaxfectin enhances immunogenicity and protective efficacy of P. yoelii circumsporozoite DNA vaccines
    Martha Sedegah
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910 7500, USA
    Vaccine 24:1921-7. 2006
    ..Clinical trials of pDNA vaccines have often used low pDNA doses based on a per kilogram weight basis. Formulation of pDNA vaccines in Vaxfectin may improve their potency in human clinical trials...
  26. pmc Persistence of protective immunity to malaria induced by DNA priming and poxvirus boosting: characterization of effector and memory CD8(+)-T-cell populations
    Martha Sedegah
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910 7500, USA
    Infect Immun 70:3493-9. 2002
    ..These results demonstrate that a heterologous priming and boosting vaccination can provide lasting protection against malaria in this model system...
  27. ncbi request reprint DNA vaccination
    Richard C Hedstrom
    Regulatory Affairs Section, DynPort Vaccine Company, Frederick, MD, USA
    Methods Mol Med 72:347-59. 2002
  28. ncbi request reprint Optimal induction of antigen-specific CD8+ T cell responses requires bystander cell participation
    Gary T Brice
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    J Leukoc Biol 72:1164-71. 2002
    ..Our data further establish a novel role for NK cells in the activation of antigen-specific immune responses...
  29. pmc Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic data
    Denise L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910 7500, USA
    Proc Natl Acad Sci U S A 100:9952-7. 2003
    ..The data suggest that immune responses to Plasmodium are dispersed on a relatively large number of parasite antigens. These studies have implications for our understanding of immunodominance and breadth of responses to complex pathogens...
  30. ncbi request reprint Induction in humans of CD8+ and CD4+ T cell and antibody responses by sequential immunization with malaria DNA and recombinant protein
    Ruobing Wang
    Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
    J Immunol 172:5561-9. 2004
    ..The induction in humans of the three primary Ag-specific adaptive immune responses establishes a strategy for developing immunization regimens against diseases in desperate need of vaccines...
  31. pmc A small peptide (CEL-1000) derived from the beta-chain of the human major histocompatibility complex class II molecule induces complete protection against malaria in an antigen-independent manner
    Yupin Charoenvit
    Cel Sci Corporation, 8229 Boone Blvd, Suite 802, Vienna, VA 22182, USA
    Antimicrob Agents Chemother 48:2455-63. 2004
    ....
  32. ncbi request reprint Assessing antigen-specific CD8+ CTL responses in humans
    Denise L Doolan
    Malaria Program, Naval Medical Research Center, Silver Spring, MD, USA
    Methods Mol Med 72:445-56. 2002
  33. ncbi request reprint Malaria's journey through the lymph node
    Michael F Good
    Nat Med 13:1023-4. 2007
  34. ncbi request reprint Identification of humoral immune responses in protein microarrays using DNA microarray data analysis techniques
    Suman Sundaresh
    School of Information and Computer Sciences, University of California, Irvine, CA, USA
    Bioinformatics 22:1760-6. 2006
    ..These signals are a measure of antibody-antigen binding activity and provide a basis for understanding humoral immune responses to various infectious agents and supporting vaccine and diagnostic development...
  35. doi request reprint Status of malaria vaccine R&D in 2007. Malaria Vaccines for the World 2007, September 17-19th, 2007, London, UK
    Denise L Doolan
    The Queensland Institute of Medical Research, The Bancroft Centre, 300 Herston Road, PO Royal Brisbane Hospital, Brisbane QLD 4029, Australia
    Expert Rev Vaccines 6:903-5. 2007
  36. ncbi request reprint The path of discovery
    Denise L Doolan
    Queensland Institute of Medical Research, Brisbane, Queensland, Australia
    Hum Vaccin 4:324-6. 2008