James B Delehanty

Summary

Affiliation: Naval Medical Center
Country: USA

Publications

  1. pmc Self-assembled quantum dot-peptide bioconjugates for selective intracellular delivery
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, D C 20375, USA
    Bioconjug Chem 17:920-7. 2006
  2. doi request reprint Cytotoxicity of quantum dots used for in vitro cellular labeling: role of QD surface ligand, delivery modality, cell type, and direct comparison to organic fluorophores
    Christopher E Bradburne
    Center for Bio Molecular Science and Engineering, Code 6900, and Optical Sciences Division, Code 5600, U S Naval Research Laboratory, Washington, DC 20375, United States
    Bioconjug Chem 24:1570-83. 2013
  3. doi request reprint Spatiotemporal multicolor labeling of individual cells using peptide-functionalized quantum dots and mixed delivery techniques
    James B Delehanty
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
    J Am Chem Soc 133:10482-9. 2011
  4. doi request reprint Evaluation of diverse peptidyl motifs for cellular delivery of semiconductor quantum dots
    Kelly Boeneman Gemmill
    Center for Bio Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Anal Bioanal Chem 405:6145-54. 2013
  5. doi request reprint Multifunctional compact zwitterionic ligands for preparing robust biocompatible semiconductor quantum dots and gold nanoparticles
    Kimihiro Susumu
    Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 133:9480-96. 2011
  6. ncbi request reprint Quantum-dot/dopamine bioconjugates function as redox coupled assemblies for in vitro and intracellular pH sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, District of Columbia 20375, USA
    Nat Mater 9:676-84. 2010
  7. ncbi request reprint Delivering quantum dot-peptide bioconjugates to the cellular cytosol: escaping from the endolysosomal system
    James B Delehanty
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
    Integr Biol (Camb) 2:265-77. 2010
  8. doi request reprint Cellular uptake and fate of PEGylated gold nanoparticles is dependent on both cell-penetration peptides and particle size
    Eunkeu Oh
    Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, DC 20375, USA
    ACS Nano 5:6434-48. 2011
  9. doi request reprint Site-specific cellular delivery of quantum dots with chemoselectively-assembled modular peptides
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, USA
    Chem Commun (Camb) 49:7878-80. 2013
  10. doi request reprint Quantum dots and fluorescent protein FRET-based biosensors
    Kelly Boeneman
    Center for Biomolecular Science and Engineering, Washington, DC 20375, USA
    Adv Exp Med Biol 733:63-74. 2012

Collaborators

Detail Information

Publications36

  1. pmc Self-assembled quantum dot-peptide bioconjugates for selective intracellular delivery
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, D C 20375, USA
    Bioconjug Chem 17:920-7. 2006
    ..The efficacy of using peptides for selective intracellular delivery is highlighted by performing a multicolor QD labeling, where we found that the presence or absence of peptide on the QD surface controls cellular uptake...
  2. doi request reprint Cytotoxicity of quantum dots used for in vitro cellular labeling: role of QD surface ligand, delivery modality, cell type, and direct comparison to organic fluorophores
    Christopher E Bradburne
    Center for Bio Molecular Science and Engineering, Code 6900, and Optical Sciences Division, Code 5600, U S Naval Research Laboratory, Washington, DC 20375, United States
    Bioconjug Chem 24:1570-83. 2013
    ..Cumulatively, our results demonstrate that the proper tuning of QD dose, surface ligand, and delivery modality can provide robust in vitro cell labeling reagents that exhibit minimal impact on cellular viability. ..
  3. doi request reprint Spatiotemporal multicolor labeling of individual cells using peptide-functionalized quantum dots and mixed delivery techniques
    James B Delehanty
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
    J Am Chem Soc 133:10482-9. 2011
    ..Using this overall approach, QDs were targeted to both early and late endosomes, the cellular cytosol, and the plasma membrane in live cells, ultimately allowing for simultaneous five-color fluorescent imaging...
  4. doi request reprint Evaluation of diverse peptidyl motifs for cellular delivery of semiconductor quantum dots
    Kelly Boeneman Gemmill
    Center for Bio Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Anal Bioanal Chem 405:6145-54. 2013
    ..Potential applications of these peptides in the context of cellular delivery of nanoparticles and a variety of other (bio)molecules are discussed...
  5. doi request reprint Multifunctional compact zwitterionic ligands for preparing robust biocompatible semiconductor quantum dots and gold nanoparticles
    Kimihiro Susumu
    Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 133:9480-96. 2011
    ..The strong potential of these ligands to expand NP capabilities in many biological applications is highlighted...
  6. ncbi request reprint Quantum-dot/dopamine bioconjugates function as redox coupled assemblies for in vitro and intracellular pH sensing
    Igor L Medintz
    Center for Bio Molecular Science and Engineering Code 6900, US Naval Research Laboratory, Washington, District of Columbia 20375, USA
    Nat Mater 9:676-84. 2010
    ..A detailed mechanism describing the QD quenching processes that is consistent with dopamine's inherent redox chemistry is presented...
  7. ncbi request reprint Delivering quantum dot-peptide bioconjugates to the cellular cytosol: escaping from the endolysosomal system
    James B Delehanty
    Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, DC 20375, USA
    Integr Biol (Camb) 2:265-77. 2010
    ..Importantly, this QD-peptide bioconjugate elicited minimal cytotoxicity in the cell lines tested...
  8. doi request reprint Cellular uptake and fate of PEGylated gold nanoparticles is dependent on both cell-penetration peptides and particle size
    Eunkeu Oh
    Optical Sciences Division, Code 5611, U S Naval Research Laboratory, Washington, DC 20375, USA
    ACS Nano 5:6434-48. 2011
    ..The 16 nm and larger AuNPs did not enter the cells and were located at the cellular periphery. A preliminary assessment of cytotoxicity demonstrated minimal effects on cellular viability following peptide-mediated uptake...
  9. doi request reprint Site-specific cellular delivery of quantum dots with chemoselectively-assembled modular peptides
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, USA
    Chem Commun (Camb) 49:7878-80. 2013
    ..Peptides were ratiometrically assembled onto quantum dots to facilitate their specific delivery to either the plasma membrane, endosomes, the cytosol or the mitochondria of target cells. ..
  10. doi request reprint Quantum dots and fluorescent protein FRET-based biosensors
    Kelly Boeneman
    Center for Biomolecular Science and Engineering, Washington, DC 20375, USA
    Adv Exp Med Biol 733:63-74. 2012
    ..We further show that QDs and fluorescent proteins can be conjugated together intracellularly with strong potential for live-cell imaging and biosensing applications...
  11. ncbi request reprint Optimizing protein coordination to quantum dots with designer peptidyl linkers
    Kelly Boeneman Gemmill
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    Bioconjug Chem 24:269-81. 2013
    ..Implications for these findings on the development of QD-based biosensors are discussed...
  12. doi request reprint Semiconductor quantum dots in bioanalysis: crossing the valley of death
    W Russ Algar
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, 4555 Overlook Avenue, South West, Washington, DC 20375, USA
    Anal Chem 83:8826-37. 2011
    ..We describe the burgeoning role of QDs in many different fields of bioanalyses and highlight the advantages afforded by their unique physical and optical properties...
  13. pmc Selecting improved peptidyl motifs for cytosolic delivery of disparate protein and nanoparticle materials
    Kelly Boeneman
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, United States
    ACS Nano 7:3778-96. 2013
    ....
  14. doi request reprint Intracellular delivery of quantum dot-protein cargos mediated by cell penetrating peptides
    Igor L Medintz
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    Bioconjug Chem 19:1785-95. 2008
    ..Use of QD-peptide/fluorescent protein vectors may make powerful tools for understanding the mechanisms of nanoparticle-mediated drug delivery...
  15. ncbi request reprint Multidentate poly(ethylene glycol) ligands provide colloidal stability to semiconductor and metallic nanocrystals in extreme conditions
    Michael H Stewart
    Optical Sciences Division, Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 132:9804-13. 2010
    ..The improved colloidal stability of nanocrystals afforded by the tetradentate ligands was further demonstrated via the assembly of stable QD-nuclear localization signal peptide bioconjugates that promoted intracellular uptake...
  16. doi request reprint Active cellular sensing with quantum dots: transitioning from research tool to reality; a review
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC 20375, USA
    Anal Chim Acta 750:63-81. 2012
    ..A perspective on where this field is expected to develop in both the near and long-term is also provided...
  17. ncbi request reprint Peptides for specific intracellular delivery and targeting of nanoparticles: implications for developing nanoparticle-mediated drug delivery
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    Ther Deliv 1:411-33. 2010
    ..The paper concludes with a brief forward-looking perspective discussing what can be expected as this field develops in the coming years...
  18. doi request reprint Intracellular bioconjugation of targeted proteins with semiconductor quantum dots
    Kelly Boeneman
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, 4555 Overlook Avenue, S W, Washington, DC 20375, USA
    J Am Chem Soc 132:5975-7. 2010
    ..This approach can facilitate long-term monitoring of their spatio-temporal activity or, alternatively, allow engineering and in situ assembly of designer chimeric QD-fluorescent protein sensors...
  19. doi request reprint Combining chemoselective ligation with polyhistidine-driven self-assembly for the modular display of biomolecules on quantum dots
    Duane E Prasuhn
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    ACS Nano 4:267-78. 2010
    ..This modular approach for displaying peptides or DNA on QDs may be extended to other more complex biomolecules such as proteins or utilized with different types of nanoparticle materials...
  20. doi request reprint Quantum dots: a powerful tool for understanding the intricacies of nanoparticle-mediated drug delivery
    James B Delehanty
    Center for Bio Molecular Science and Engineering, SW Washington, DC 20375, USA
    Expert Opin Drug Deliv 6:1091-112. 2009
    ..Important related issues such as QD biofunctionalization and toxicity are also discussed. The paper concludes with a perspective of how this field can be expected to develop in the future...
  21. ncbi request reprint A comparison of microscope slide substrates for use in transfected cell microarrays
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375 5348, USA
    Biosens Bioelectron 20:773-9. 2004
    ..976). The transfection efficiency of HEK 293T/17 cells varied in accord with the mean spot diameter, demonstrating that the uptake of DNA was a function of the local DNA concentration on each substrate...
  22. ncbi request reprint Transfected cell microarrays for the expression of membrane-displayed single-chain antibodies
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, D C 20375 5348, USA
    Anal Chem 76:7323-8. 2004
    ..More generally, our work details the utility of transfected cell microarrays in mediating the functional characterization of expressed membrane receptor proteins...
  23. ncbi request reprint Selection of cholera toxin specific IgNAR single-domain antibodies from a naïve shark library
    Jinny L Liu
    Center for Bio Molecular Science and Engineering, US Naval Research Laboratory, Washington, DC 20375, USA
    Mol Immunol 44:1775-83. 2007
    ..The successful isolation of target specific sdAbs from one of our non-biased NAR libraries, demonstrate their ability to provide binders against an unacquainted antigen of interest...
  24. pmc Facile generation of heat-stable antiviral and antitoxin single domain antibodies from a semisynthetic llama library
    Ellen R Goldman
    Center for Bio Molecular Science and Engineering, US Naval Research Laboratory, Washington, DC 20375, USA
    Anal Chem 78:8245-55. 2006
    ..The ability to rapidly select such rugged antibodies will enhance the reliability of immunoassays by extending shelf life and the capacity to function in hostile environments...
  25. ncbi request reprint Binding and neutralization of lipopolysaccharides by plant proanthocyanidins
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC 20375, USA
    J Nat Prod 70:1718-24. 2007
    ..Our results identify PACs as a new class of LPS-binding compound and suggest that they have potential utility in applications that necessitate either the purification and removal of LPS or the in vivo neutralization of LPS...
  26. doi request reprint Immobilized proanthocyanidins for the capture of bacterial lipopolysaccharides
    Brandy J Johnson
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, D C 20375 5348, USA
    Anal Chem 80:2113-7. 2008
    ..Molar comparison of polymyxin B to proanthocyanidins indicated that the Sepharose immobilized PACs have a binding affinity for LPS similar to that of polymyxin B...
  27. doi request reprint Delivering quantum dots into cells: strategies, progress and remaining issues
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Code 6900, U S Naval Research Laboratory, Washington, DC, USA
    Anal Bioanal Chem 393:1091-105. 2009
    ..Finally, we conclude by providing a perspective of how this field can be expected to develop in the future...
  28. doi request reprint Transfected cell microarrays for the expression of membrane-displayed single-chain antibodies
    Baochuan Lin
    United States Naval Research Laboratory, Center for Bio Molecular Science and Engineering, Washington, DC, USA
    Methods Mol Biol 706:119-37. 2011
    ..This approach provides for the high-throughput functional analysis of many different proteins in parallel and can be considerably more informative and cost-effective relative to more traditional protein expression techniques...
  29. ncbi request reprint Enhancing the stability and biological functionalities of quantum dots via compact multifunctional ligands
    Kimihiro Susumu
    Division of Optical Sciences and Center for Bio Molecular Science and Engineering, U S Naval Research Laboratory, Washington, D C 20375, USA
    J Am Chem Soc 129:13987-96. 2007
    ..The new functional surface ligands described here provide not only stable and highly water-soluble QDs but also simple and easy access to various biological entities...
  30. ncbi request reprint Printing functional protein microarrays using piezoelectric capillaries
    James B Delehanty
    Center for Biomolecular Sciences and Engineering, Naval Research Laboratory, Washington, DC, USA
    Methods Mol Biol 264:135-43. 2004
    ..Further, this method results in the deposition of capture antibodies that adopt a more uniform spot morphology...
  31. doi request reprint Elaborate nanoparticle-based traps for catching cytosolic players in the act
    James B Delehanty
    Center for Bio Molecular Science and Engineering, US Naval Research Laboratory, Code 6900, Washington DC 20375 USA
    Chembiochem 13:30-3. 2012
    ..The system uses the rapid, innate self-assembly of ferritin, expressing bait and prey molecules, into ordered nanoclusters whose morphology is tracked by using fluorescence microscopy...
  32. ncbi request reprint Antiviral properties of cobalt(III)-complexes
    James B Delehanty
    Center for Bio Molecular Science and Engineering, 4555 Overlook Avenue SW, Washington, DC 20375, USA
    Bioorg Med Chem 16:830-7. 2008
    ..We discuss our findings within the context of the ability to further functionalize Co(III)hexammine to render it a highly specific antiviral therapeutic reagent...
  33. ncbi request reprint Identification of important residues in metal-chelate recognition by monoclonal antibodies
    James B Delehanty
    Department of Ophthalmology, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112, USA
    Biochemistry 42:14173-83. 2003
    ....
  34. ncbi request reprint Method for printing functional protein microarrays
    James B Delehanty
    Naval Research Laboratory, Washington, DC, USA
    Biotechniques 34:380-5. 2003
    ..The implications of these results on the future development of protein biochips are discussed...
  35. ncbi request reprint A microarray immunoassay for simultaneous detection of proteins and bacteria
    James B Delehanty
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375 5348, USA
    Anal Chem 74:5681-7. 2002
    ..2 x 10(4) cfu/mL, respectively. The assays presented here are very fast, as compared to previously published methods for measuring antibody-antigen interactions using microarrays (minutes versus hours)...
  36. ncbi request reprint Monoclonal antibodies that recognize minimal differences in the three-dimensional structures of metal-chelate complexes
    R Mark Jones
    Department of Ophthalmology, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Bioconjug Chem 13:408-15. 2002
    ..Antibody affinity correlated well with the extent of total structural difference for these metal-EDTA complexes...