Joshua Zimmerberg

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc "Entropic traps" in the kinetics of phase separation in multicomponent membranes stabilize nanodomains
    V A J Frolov
    Frumkin Institute of Electrochemistry, Russian Academy of Sciences, Moscow, Russia
    Biophys J 91:189-205. 2006
  2. pmc Cytoplasmic free Ca2+ is essential for multiple steps in malaria parasite egress from infected erythrocytes
    Svetlana Glushakova
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, 20892, USA
    Malar J 12:41. 2013
  3. ncbi request reprint Regulated secretion: SNARE density, vesicle fusion and calcium dependence
    Jens R Coorssen
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 1855, USA
    J Cell Sci 116:2087-97. 2003
  4. pmc An adhesion-based method for plasma membrane isolation: evaluating cholesterol extraction from cells and their membranes
    Ludmila Bezrukov
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Anal Biochem 394:171-6. 2009
  5. pmc Insulin regulates fusion of GLUT4 vesicles independent of Exo70-mediated tethering
    Vladimir A Lizunov
    Laboratory of Cellular and Molecular Biophysics, Program on Physical Biology, Eunice Kennedy Schriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    J Biol Chem 284:7914-9. 2009
  6. pmc Insulin stimulates fusion, but not tethering, of GLUT4 vesicles in skeletal muscle of HA-GLUT4-GFP transgenic mice
    Vladimir A Lizunov
    Program in Physical Biology, National Institute of Child Health and Human Development National Institutes of Health, Bethesda, MD, USA
    Am J Physiol Endocrinol Metab 302:E950-60. 2012
  7. pmc Insulin controls the spatial distribution of GLUT4 on the cell surface through regulation of its postfusion dispersal
    Karin G Stenkula
    Experimental Diabetes, Metabolism, and Nutrition Section, Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Metab 12:250-9. 2010
  8. pmc Cholesterol promotes hemifusion and pore widening in membrane fusion induced by influenza hemagglutinin
    Subrata Biswas
    Laboratory of Cellular and Molecular Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Gen Physiol 131:503-13. 2008
  9. pmc Insulin regulates Glut4 confinement in plasma membrane clusters in adipose cells
    Vladimir A Lizunov
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 8:e57559. 2013
  10. pmc Initial size and dynamics of viral fusion pores are a function of the fusion protein mediating membrane fusion
    Ilya Plonsky
    Laboratory of Cellular and Molecular Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 1855, U S A
    Biol Cell 100:377-86. 2008

Collaborators

Detail Information

Publications51

  1. pmc "Entropic traps" in the kinetics of phase separation in multicomponent membranes stabilize nanodomains
    V A J Frolov
    Frumkin Institute of Electrochemistry, Russian Academy of Sciences, Moscow, Russia
    Biophys J 91:189-205. 2006
    ..The theoretical critical value of line tension needed to rapidly form large rafts is in accord with the experimental estimate from the curvatures of budding domains in giant unilamellar vesicles...
  2. pmc Cytoplasmic free Ca2+ is essential for multiple steps in malaria parasite egress from infected erythrocytes
    Svetlana Glushakova
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, 20892, USA
    Malar J 12:41. 2013
    ..This study analysed the involvement of cytoplasmic free Ca2+ in infected erythrocytes during the multistep egress programme of malaria parasites...
  3. ncbi request reprint Regulated secretion: SNARE density, vesicle fusion and calcium dependence
    Jens R Coorssen
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 1855, USA
    J Cell Sci 116:2087-97. 2003
    ....
  4. pmc An adhesion-based method for plasma membrane isolation: evaluating cholesterol extraction from cells and their membranes
    Ludmila Bezrukov
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Anal Biochem 394:171-6. 2009
    ..Determination of the cholesterol/phospholipid ratio from intact cells does not reflect methyl-beta-cyclodextrin plasma membrane extraction properties...
  5. pmc Insulin regulates fusion of GLUT4 vesicles independent of Exo70-mediated tethering
    Vladimir A Lizunov
    Laboratory of Cellular and Molecular Biophysics, Program on Physical Biology, Eunice Kennedy Schriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
    J Biol Chem 284:7914-9. 2009
    ..Taken together, our data suggest that fusion of GLUT4 vesicles is the rate-limiting step regulated by insulin downstream of Exo70-mediated tethering...
  6. pmc Insulin stimulates fusion, but not tethering, of GLUT4 vesicles in skeletal muscle of HA-GLUT4-GFP transgenic mice
    Vladimir A Lizunov
    Program in Physical Biology, National Institute of Child Health and Human Development National Institutes of Health, Bethesda, MD, USA
    Am J Physiol Endocrinol Metab 302:E950-60. 2012
    ..Most likely, pretethered GLUT4 structures are responsible for the initial phase of insulin-stimulated exocytosis...
  7. pmc Insulin controls the spatial distribution of GLUT4 on the cell surface through regulation of its postfusion dispersal
    Karin G Stenkula
    Experimental Diabetes, Metabolism, and Nutrition Section, Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Metab 12:250-9. 2010
    ..The data fit a new kinetic model that features multifunctional clusters as intermediates of exocytosis and endocytosis...
  8. pmc Cholesterol promotes hemifusion and pore widening in membrane fusion induced by influenza hemagglutinin
    Subrata Biswas
    Laboratory of Cellular and Molecular Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Gen Physiol 131:503-13. 2008
    ..The results of this study support the hypothesis that host cell cholesterol acts at two stages in membrane fusion: (1) early, prior to fusion pore opening, and (2) late, during fusion pore expansion...
  9. pmc Insulin regulates Glut4 confinement in plasma membrane clusters in adipose cells
    Vladimir A Lizunov
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 8:e57559. 2013
    ..All together these three effects of insulin shift most of the PM GLUT4 from clustered to dispersed states. GLUT4 confinement in clusters represents a novel kinetic mechanism for insulin regulation of glucose homeostasis...
  10. pmc Initial size and dynamics of viral fusion pores are a function of the fusion protein mediating membrane fusion
    Ilya Plonsky
    Laboratory of Cellular and Molecular Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 1855, U S A
    Biol Cell 100:377-86. 2008
    ....
  11. doi request reprint Progressive ordering with decreasing temperature of the phospholipids of influenza virus
    Ivan V Polozov
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, 10D14, 10 Center Drive MSC 1855, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Chem Biol 4:248-55. 2008
    ..Viral fusion appears to be uncorrelated to ordered lipid content. Lipid ordering may contribute to viral stability at lower temperatures, which has recently been found to be critical for airborne transmission...
  12. pmc Irreversible effect of cysteine protease inhibitors on the release of malaria parasites from infected erythrocytes
    Svetlana Glushakova
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Microbiol 11:95-105. 2009
    ....
  13. ncbi request reprint Membrane fusion of secretory vesicles of the sea urchin egg in the absence of NSF
    Tim Whalley
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 117:2345-56. 2004
    ..In contrast, isolated CVs, though all capable of Ca(2+)-triggered homotypic fusion, contain less than one hexamer of NSF per CV. Thus NSF is not a required component of the CV fusion machinery...
  14. pmc Vesicle formation by self-assembly of membrane-bound matrix proteins into a fluidlike budding domain
    Anna V Shnyrova
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 179:627-33. 2007
    ..Thus, matrix proteins implement domain-driven mechanism of budding, which suffices to control the shape of these proteolipid vesicles...
  15. pmc GTPase cycle of dynamin is coupled to membrane squeeze and release, leading to spontaneous fission
    Pavel V Bashkirov
    Program on Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development NICHD, Bethesda, MD 20892 1855, USA
    Cell 135:1276-86. 2008
    ..We propose that dynamin transmits GTP's energy to periodic assembling of a limited curvature scaffold that brings lipids to an unstable intermediate...
  16. pmc Domain-driven morphogenesis of cellular membranes
    Anna V Shnyrova
    Laboratory of Cellular and Molecular Biology, Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 1855, USA
    Curr Biol 19:R772-80. 2009
    ..Here, we analyze the essential role of proteo-lipid membrane domains in conducting and coordinating morphological remodeling in cells...
  17. pmc Chapter 4 - Reconstitution of membrane budding with unilamellar vesicles
    Anna V Shnyrova
    Laboratory of Cellular and Molecular Biology, Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland, USA
    Methods Enzymol 464:55-75. 2009
    ..The assays may be applied for direct reconstitution of the on-membrane action of cellular proteins involved in membrane curvature induction upon binding in vivo...
  18. pmc Membranes of the world unite!
    Leonid V Chernomordik
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 175:201-7. 2006
    ..In this mini-review, we discuss how proteins can mediate each of the three major stages in the fusion pathway: contact, hemifusion, and the opening of an expanding fusion pore...
  19. ncbi request reprint De novo sequencing of peptides using MALDI/TOF-TOF
    Alfred L Yergey
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA
    J Am Soc Mass Spectrom 13:784-91. 2002
    ..These results show both the power of the present approach and the need for using de novo methods more frequently than may be otherwise appreciated...
  20. pmc Thermodynamics of interleaflet cavitation in lipid bilayer membranes
    Shay M Rappaport
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 87:022715. 2013
    ..It is found that the optimized cavity is almost spherical at large bubble volumes, while at small volumes the cavity has a lenslike shape. The optimized shape is used to analyze the minimum work of IMC...
  21. pmc Trapping of diffusing particles by clusters of absorbing disks on a reflecting wall with disk centers on sites of a square lattice
    Alexander M Berezhkovskii
    Mathematical and Statistical Computing Laboratory, Division for Computational Bioscience, Center for Information Technology, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Chem Phys 138:064105. 2013
    ..The comparison made for 18 clusters of various shapes and sizes shows good agreement between the theoretical predictions and numerical results...
  22. pmc Isolation and ultrastructural characterization of squid synaptic vesicles
    Gulcin Pekkurnaz
    National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    Biol Bull 220:89-96. 2011
    ..Negative-stain tomography demonstrates directly that vesicles flatten during the drying step of negative staining, and furthermore shows details of individual vesicles and other proteins at the vesicle surface...
  23. pmc Quantification of malaria parasite release from infected erythrocytes: inhibition by protein-free media
    Svetlana Glushakova
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Malar J 6:61. 2007
    ..Intracellular malaria parasites leave their host erythrocytes to infect neighbouring cells after each cycle of asexual replication. No method is currently available for the direct quantification of parasite release...
  24. ncbi request reprint Neuroscience. Synaptic membranes bend to the will of a neurotoxin
    Joshua Zimmerberg
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 1855, USA
    Science 310:1626-7. 2005
  25. pmc New stages in the program of malaria parasite egress imaged in normal and sickle erythrocytes
    Svetlana Glushakova
    Program in Physical Biology, Eunice Kennedy Schriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Biol 20:1117-21. 2010
    ..Separated parasites leave the erythrocyte by breaching its membrane, weakened by putative digestion of erythrocyte cytoskeleton and membrane poration...
  26. pmc Shear forces during blast, not abrupt changes in pressure alone, generate calcium activity in human brain cells
    Rea Ravin
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 7:e39421. 2012
    ..3 to 0.7 Pa, were present at the cell surface. The probability of cellular injury in response to a shockwave was low and cell survival was unaffected 20 hours after shockwave exposure...
  27. pmc A negative stain for electron microscopic tomography
    Andrea Fera
    Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, Bethesda, MD 20892, USA
    Microsc Microanal 18:331-5. 2012
    ..Negative stain tomography with the appropriate stain yields detailed images of individual molecules in their normal setting on the surface of the influenza A virus...
  28. ncbi request reprint Membrane transformation during malaria parasite release from human red blood cells
    Svetlana Glushakova
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Biol 15:1645-50. 2005
    ..These results, together with the modulation of release by osmotic stress, suggest a pathway of parasite release that features a biochemically altered erythrocyte membrane that folds after pressure-driven rupture of membranes...
  29. ncbi request reprint Cellular biophysics: bacterial endospore, membranes and random fluctuation
    Vladimir Lizunov
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892 1855, USA
    Curr Biol 16:R1025-8. 2006
    ....
  30. doi request reprint Flexible scaffolding made of rigid BARs
    Vadim A Frolov
    Program on Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 1855, USA
    Cell 132:727-9. 2008
    ..In this issue, Frost et al. (2008) reveal how collective twisting of rigid F-BAR domains on a soft membrane surface may lead to different membrane curvatures...
  31. ncbi request reprint Membrane biophysics
    Joshua Zimmerberg
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 1855, USA
    Curr Biol 16:R272-6. 2006
  32. pmc Paradoxical lipid dependence of pores formed by the Escherichia coli alpha-hemolysin in planar phospholipid bilayer membranes
    Laura Bakas
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 91:3748-55. 2006
    ..HlyA also appeared to thicken bilayers to which it was added. We discuss these results in terms of models for proteolipidic pores...
  33. pmc Impaired tethering and fusion of GLUT4 vesicles in insulin-resistant human adipose cells
    Vladimir A Lizunov
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA
    Diabetes 62:3114-9. 2013
    ..This altered insulin responsiveness is consistent with the hypothesis that adipose cellular dysfunction is a primary contributor to systemic metabolic dysfunction. ..
  34. pmc GREG cells, a dysferlin-deficient myogenic mouse cell line
    Glen W Humphrey
    Program in Physical Biology, Eunice Kennedy Schriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Exp Cell Res 318:127-35. 2012
    ..We discuss the possibility that the observed heterogeneity in membrane resealing represents genetic compensation for dysferlin deficiency...
  35. pmc Insulin stimulates the halting, tethering, and fusion of mobile GLUT4 vesicles in rat adipose cells
    Vladimir A Lizunov
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 169:481-9. 2005
    ..It is likely that the primary mechanism of insulin action in GLUT4 translocation is to stimulate tethering and fusion of trafficking vesicles to specific fusion sites in the PM...
  36. pmc Communication: Clusters of absorbing disks on a reflecting wall: competition for diffusing particles
    Alexander M Berezhkovskii
    Mathematical and Statistical Computing Laboratory, Division for Computational Bioscience, Center for Information Technology, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Chem Phys 136:211102. 2012
    ..By replacing the cluster with an effective uniformly absorbing spot, we derive a simple formula for the rate constant that characterizes the trapping. The formula shows how the rate constant depends on the size and shape of the cluster...
  37. pmc Line tension and interaction energies of membrane rafts calculated from lipid splay and tilt
    Peter I Kuzmin
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 88:1120-33. 2005
    ..This barrier can kinetically stabilize the rafts against merger. Our physical theory thus quantifies conditions that allow rafts to form, and further, defines the parameters that control raft merger...
  38. ncbi request reprint A new method for culturing Plasmodium falciparum shows replication at the highest erythrocyte densities
    Tao Li
    NASA NIH Center for Three Dimensional Tissue Culture, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Infect Dis 187:159-62. 2003
    ..falciparum can replicate at packed erythrocyte densities suggests that this system may be useful for study of the pathogenesis of fatal cerebral malaria, of which one feature is densely packed blood cells in brain microvasculature...
  39. doi request reprint ER biogenesis: self-assembly of tubular topology by protein hairpins
    Anna Shnyrova
    Program on Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 1855, USA
    Curr Biol 18:R474-6. 2008
    ..Two of these proteins are sufficient to form tubular membrane networks from pure phospholipid vesicles, thus revealing a new paradigm of ER morphogenesis...
  40. ncbi request reprint How proteins produce cellular membrane curvature
    Joshua Zimmerberg
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 1855, USA
    Nat Rev Mol Cell Biol 7:9-19. 2006
    ..We describe membrane elastic properties in order to formulate the structural and energetic requirements of proteins and lipids that would enable them to work together to generate the membrane shapes seen during intracellular trafficking...
  41. pmc Nanoscale 3D cellular imaging by axial scanning transmission electron tomography
    Martin F Hohmann-Marriott
    National Institute of Biomedical Imaging and Bioengineering, Bethesda, Maryland, USA
    Nat Methods 6:729-31. 2009
    ..We demonstrated the method by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum...
  42. pmc Immune suppression of human lymphoid tissues and cells in rotating suspension culture and onboard the International Space Station
    Wendy Fitzgerald
    NASA NIH Center for Three Dimensional Tissue Culture, Laboratory of Cellular and Molecular Biophysics, Program in Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    In Vitro Cell Dev Biol Anim 45:622-32. 2009
    ..This phenomenon may reflect immune dysfunction observed in astronauts during space flights. If so, the ex vivo system described above can be used to understand cellular and molecular mechanisms of this dysfunction...
  43. pmc Quantitative electron microscopy and fluorescence spectroscopy of the membrane distribution of influenza hemagglutinin
    Samuel T Hess
    Laboratory for Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 169:965-76. 2005
    ..This work rules out the tested hypothesis for HA over the accessible length scales, yet shows clearly how the spatial distribution of HA depends on lipid composition...
  44. ncbi request reprint The physical chemistry of biological membranes
    Joshua Zimmerberg
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Chem Biol 2:564-7. 2006
    ..With increasing sophistication of investigative tools, there is now a growing appreciation for lipid diversity and for the role of individual lipids and specific lipid-protein interactions in membrane structure and function...
  45. ncbi request reprint Membrane curvature: how BAR domains bend bilayers
    Joshua Zimmerberg
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Biol 14:R250-2. 2004
    ..BAR resembles a banana that binds membranes electrostatically through its positively charged, concave surface...
  46. ncbi request reprint Endocytosis: curvature to the ENTH degree
    Ralph Nossal
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Biol 12:R770-2. 2002
    ..Recent work has shown that the protein epsin 1 induces highly curved lipidic structures when added with clathrin to appropriate lipid mixtures. This property may be a critical factor in the 'curvature stress cycle' of membrane trafficking...
  47. pmc Cytotoxicity mediated by the Fas ligand (FasL)-activated apoptotic pathway in stem cells
    Julia Mazar
    Program on Physical Biology, NICHD, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 284:22022-8. 2009
    ....
  48. pmc Dynamics of putative raft-associated proteins at the cell surface
    Anne K Kenworthy
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 18T, Rm 101, 18 Library Dr, Bethesda, MD 20895, USA
    J Cell Biol 165:735-46. 2004
    ..Thus, raft association is not the dominant factor in determining long-range protein mobility at the cell surface...
  49. ncbi request reprint Quantitative femto- to attomole immunodetection of regulated secretory vesicle proteins critical to exocytosis
    Jens R Coorssen
    Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 1855, USA
    Anal Biochem 307:54-62. 2002
    ..The results indicate that sea urchin egg secretory vesicles and synaptic vesicles have markedly similar SNARE densities...
  50. ncbi request reprint Elongated membrane zones boost interactions of diffusing proteins
    Joshua Zimmerberg
    Program in Physical Biology, Eunice Kennedy Schriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 146:501-3. 2011
    ..2011) now show that actomyosin and tubulin interact to establish long, thin diffusion corridors, thereby increasing the effective concentration of select membrane proteins to promote their interactions and modulate signaling...
  51. pmc Long, saturated chains: tasty domains for kinases of insulin resistance
    Vladimir Lizunov
    Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 21:604-6. 2011
    ..Holzer et al. (2011) recently propose in Cell that expansion of intracellular membrane microdomains induced by saturated FA recruit and activate c-Src for JNK activation...